Browsing by Author "Kaya, Seval"

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Comparison of the Gonadoprotective Functions of Nigella Sativa Oil, Metformin and Vitamin E on Streptozotocin-Induced Diabetic Testis
(Edizioni Minerva Medica, 2025) Kaya, Seval; Seker, Ugur; Ozmen, Mehmet F.; Ayaz, Hayat; Erdogan, Gamze; Nergiz, Yusuf; Department of Basic Medical Sciences / Temel Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
BACKGROUND: Changes in blood glucose levels in DM cause many complications. We aimed to make a detailed comparison study on the protective effects of Nigella sativa oil, metformin, and vitamin E on the histopathologic effects of diabetes on the male reproductive system. METHODS: In the experiment, 30 rats were divided equally into 5 groups: Control, DM, DM+NS, DM+M and DM+E. To induce diabetes in the experimental groups, a one-time injection of 45 mg/kg Streptozotocin was administered. Rats with blood glucose levels above 250 mg/dl were included in the experiment. The experiment period lasted 8 weeks. Weekly body weight of the rats, reproductive organ weights at the end of the experiment, spermatologic data, immunohistochemical staining, TUNEL assay staining and morphometric evaluation were performed. RESULTS: According to Johnsen Biopsy Score and Seminiferous Tubule diameter data, the DM+NS group had similar results to the control group, indicating that Nigella sativa oil preserved the seminiferous tubule diameter structure better than vitamin E and metformin. There were head, neck and tail defects in the diabetes group. Based on the results of the applications carried out to evaluate apoptosis and proliferation in testicular tissue, it can be concluded that the treated diabetic groups, especially the Nigella sativa oil, improved the structure of spermatogenic tubules and also reduced the number of apoptotic cells, as shown by the results of the TUNEL test (P<0.05). CONCLUSIONS: We think that Nigella sativa oil is more effective on the mechanism of diabetes than other active substances and regulates metabolic changes and may be an effective therapeutic agent for diabetes treatment.
Neuroprotective Potential of a Novel Soluble Guanylate Cyclase Stimulator the Riociguat Alone or in a Combination Manner With Resveratrol in Experimental Stroke Model in Rats
(Soc Chilena Anatomia, 2024) Aslanoglu, Baris; Kaya, Seval; Gunara, Sezer Onur; Atlas, Burak; Seker, Ugur; Guzel, Baris Can; Turan, Yahya; Department of Basic Medical Sciences / Temel Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
In this study we aimed to examine the effect of novel vasodilatory drug Riociguat co-administration along resveratrol to recover neurodegeneration in experimental stroke injury. For that purpose, thirty-five adult female rats were divided into groups five (Control, MCAO, MCAO + R, MCAO + BAY, MCAO + C) of seven animals in each. Animals in Control group did not expose to any application during the experiment and sacrificed at the end of the study. Rats in the rest groups exposed to middle cerebral artery occlusionO) (MCAinduced ischemic stroke. MCAO + R group received 30 mg/kg resveratrol, and MCAO + BAY group received 10 mg/kg Riociguat. The MCAO + C group received both drugs simultaneously. The drugs were administered just before the reperfusion, and the additional e doses weradministered 24h, and 48h hours of reperfusion. All animals in this study were sacrificed at the 72nd hour of experiment. Total brainsreceived were for analysis. Results of this experiment indicated that MCAO led to severe injury in cerebral structure. Bax, IL-6 and IL-1 ss levelstissue were upregulated, but anti-apoptotic Bcl-2 immunoexpression was suppressed (p<0.05). In resveratrol and Riociguat treated animals, the neurodegenerations and apoptosis and inflammation associated protein expressions were improved compared to MCAO group, mosbut the success was obtained in combined treatment exposed animals in MCAO + C group. This study indicated that the novel solubleate guany cyclase stimulator Riociguat is not only a potent neuroprotective drug in MCAO induced stroke, but also synergistic administratio of Riociguat along with resveratrol have potential to increase the neuroprotective effect of resveratrol in experimental cerebral strokeosed rats.