Derviş, Sibel

Derviş, Sibel

Name Variants

Derviş, S.
Dervis, Sibel

Job Title

Prof. Dr.

Main Affiliation

Department of Organic Agriculture / Organik Tarım Bölümü

Status

Current Staff

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Scholarly Output

88

Articles

55

Citation Count

0

Supervised Theses

0 Scholarly Output Search Results

Now showing 1 - 10 of 62

Postharvest fungal pathogens of apple in Nigde Province, Turkey
(2022) Derviş, Sibel; Özden, Mustafa; Derviş, Sibel
Apples are an economically important horticultural product worldwide. They can be stored under low temperature for long periods of time (up to 10 months). However, fungal decay is a major problem in long-term storage and causes serious postharvest fruit loses around the world, including Turkey. This decay is caused by several fungi with different infection mechanisms favored by different conditions. Therefore, it is crucial to know which fungal pathogens are the primary cause of any decay in order to develop innovative control strategies. For this reason, the aim of this study was to sample decaying apples from cold storage facilities in Niğde Province, Turkey (an important apple-producing province) to isolate and identify the pathogens. A total of 395 visually infected apples were sampled from 18 cold storage facilities in the 2020/21 production season. Culture morphology of the isolates was examined, isolates were morphologically identified at the genus level. The results showed that the identified dominant genera considered of major contributors to fruit spoilage belonged to genus Penicillium (76.5%), followed by Alternaria (19.0%). Other fungal pathogens, including species of Botrytis, Cadophora, Cladosporium and Fusarium had low abundance. Pathogenicity testing and molecular characterization of the isolates is in progress.
Fungi isolated from cankered tissues of declining apricot trees in Malatya and Elazığ provinces of Turkey
(2017) Derviş, Sibel; Çiftçi, Osman; Derviş, Sibel
Surveys were carried out in apricot (Prunus armeniaca L.) production areas of Malatya and Elazığ provinces from April to November in 2015 and 2016. Fungal and oomycetous diseases causing dieback and decline symptoms were investigated and locations where the diseases were prevalent were determined according to the districts in these provinces. Nine and 40 orchards were visited in Elazığ and Malatya during the course of the surveys. A total of 665 out of 5750 apricot trees were checked and the disease incidence was found to be 44% in the surveyed orchards. Out of isolates obtained from root and crown tissues of symptomatic trees, isolates obtained from cankered tissues were characterized according to their morphological characteristics. Genomic DNA was extracted from representative isolates. The internal transcribed spacer (ITS) region of rDNA was amplified using the ITS6/ITS4 primer pair and sequenced and submitted to GenBank. NCBI BLAST results showed 98 to 100% similarity with the ITS sequences of many Clonostachys rosea f. rosea (Link : Fr.) Schroers et. al. 1999 (Ascomycetes, Hypocreales), Sarocladium kiliense (Grütz) Summerb. 2011 (Ascomycetes, Incertae sedis) (Syn: Acremonium kiliense), Phoma sp. (Ascomycetes, Pleosporales), Entoleuca spp. (Ascomycetes, Xylariales) strains deposited in NCBI GenBank. The sequences were submitted to GenBank and given accession numbers were MF536537 and MF536538 for C. rosea, MF536539 for S. kiliense, MF536540 and MF536541 for Phoma spp., and MF536542, MF536543, MF536544 and MF536545 for Entoleuca spp. isolates. Moreover, Verticillium dahliae and Macrophomina phaseolina were also isolated from inner tissues of necrotic branches and morphologically identified. However, pathogenicity of these isolates needs further investigations. If some isolates were not pathogenic, their endophytic or hperparasitic characteristics against pathogenic ones should be tested in order to fully exploit their potential for use as biological control agents.
Phytopythium litorale: A novel killer pathogen of plane (Platanus orientalis) causing canker stain and root and collar rot
(Plant Disease, 2020) Derviş, Sibel; Türkölmez, Şahimerdan; Çiftçi, Osman; Özer, Göksel; Serçe, Çiğdem Ulubaş; Dikilitaş, Murat
Decline symptoms associated with lethal stem and branch canker stain along with root and collar rots were observed on 5- to 7-year-old roadside oriental plane trees (Platanus orientalis) in Diyarbakır, Turkey. Above-ground symptoms included leaf necrosis, leaf curling, extensive bluish or blackish staining of shoots, branches, stem bark, and wood surfaces, as well as stem cankers and exfoliation of branch bark scales. A general decline of the trees was distinctly visible from a distance. A Phytophthora/ Pythium-like oomycete species with globose to ovoid, often papillate and internally proliferating sporangia was consistently isolated from the fine and coarse roots and stained branch parts and shoots. The pathogen was identified as Phytopythium litorale based on several morphological features. Partial DNA sequences of three loci, including nuclear rDNA internal transcribed spacer (ITS) and the large ribosomal subunit (LSU), and mitochondrial cytochrome c oxidase subunit II (coxII) confirmed the morphological identification. All P. litorale isolates were homothallic, developing gametangia, ornamented oogonia with elongate to lobate antheridia. Pathogenicity of P. litorale was tested by inoculation on excised shoots and by root inoculation on seedlings. P. litorale produced large lesions and blights on shoots in just 5 days and killed 100% of the seedlings in a month. This paper presents the first confirmed report of P. litorale as an important pathogen on a plant species causing branch and stem cankers, and root and collar rot, in and on P. orientalis, resulting in a rapid decline of trees and suggesting a threat to plane.
Lasiodiplodia theobromae and Neoscytalidium dimidiatum associated with grafted walnut (Juglans regia L.) decline in Turkey
(SpringerLink, 2023) Derviş, Sibel; Osman Çiftçi, Göksel Özer, Şahimerdan Türkölmez, Sibel Derviş
Surveys of canker diseases in 26 walnut orchards were conducted in the southeastern Turkish provinces of Batman, Diyarbakır, and Şanlıurfa in 2020. Decline symptoms on trees were most severe in the trunks of grafted trees grown on unproductive local rootstocks with Chandler and Franquette scions throughout the surveyed areas, and samples were taken from those trees. Lasiodiplodia theobromae was identifed from the cankers on the trees of the seven orchards, while Neoscytalidium dimidiatum was identifed from the other 19 orchards based on morphological characteristics. DNA sequencing analysis of 10 representative isolates using the ITS1-5.8S-ITS2 (ITS), the large subunit (LSU), and the translation elongation factor 1-alpha (EF-1α) genes confrmed the identifcation of the causal fungi. Pathogenicity tests on 2 year-old Chandler seedlings revealed that L. theobromae and N. dimidiatum produced lesion length averages of 7–8 cm and 15–25 cm after 3 weeks of stem-inoculation, respectively. Koch’s postulates were confrmed by successfully reisolating the fungi only from plants inoculated. This is the frst study to document L. theobromae as the causal agent of walnut tree decline and cankers in Turkey.
Farklı Buğday Genotiplerinden İzole Edilen Endofitik Bakterilerin Fusarium Culmorum'a Karşı Antagonistik Aktivitelerinin Belirlenmesi
(2024) Derviş, Sibel; Aktaş, Hüsnü; Özer, Göksel; Aktaş, Hüsnü; Keske, Mehmet Ata
Bu araştırmada, farklı buğday genotiplerinden elde edilen endofitik bakterilerin, fizyolojik ve biyokimyasal özelliklerinin ile enzim aktivitelerinin yanısıra buğdayda kök ve kökboğazı çürüklüğüne neden olan Fusarium culmorum’a karşı etkinliğinin değerlendirilmesi amaçlanmıştır. İzolatların F. culmorum’a karşı ikili kültür testlerinden elde edilen sonuçlara göre inhibisyon oranı %80.56- %13.90 arasında değişiklik göstermiştir. In vitro koşullarda Fusarium culmorum’a karşı inhibisyon oranı %80.59 oranıyla Bacillus subtilis (MM11), %69.41 oranıyla Stenotrophomonas maltophilia (EY5) ve %61.10 oranıyla Enterobacter sp. (MY3) en etkili izolatlar olmuştur. Amilaz, selülaz, fosfataz, ACC deaminaz ve siderofor üretiminde Pseudomonas putida (EM9) ve Pseudomonas orientalis (MM21) izolatları tüm testte pozitif sonuç vermiştir. Umut vadeden altı izolat 16S rRNA geninin sekans analizi kullanılarak tanımlanmıştır. Bakteri suşlarının F. culmorum’a karşı etkinliğini değerlendirmek için iklim odası koşullarında (in vivo) saksı denemeleri kurulmuştur. Sonuçlar, Enterobacter sp., S. maltophilia ve B. subtilis (MY3+EY5+MM11) kombinasyonunun hastalık şiddeti, bitki boyu, yaş ağırlık, kuru ağırlık, kök yaş ağırlığı ve kök kuru ağırlığı açısından en uygun sonuçları verdiğini göstermiştir. Stenotrophomonas rhizophila, P. putida ve P. orientalis (EY1+EM9+MM21) kombinasyonu da oldukça etkili olmuştur. Etkili bakteri suşlarının kullanılmasının, çevresel etkilerini en aza indirirken, kimyasal gübre ve pestisitlere olan bağımlılığı ve bunlarla ilişkili maliyetleri azaltması beklenmektedir. Ek doğrulama prosedürlerinin ardından bu suşların, ticari uygulamalar için potansiyelinin olduğu düşünülmektedir. Bulgular, F. culmorum’a karşı çeşitli endofitik bakterilerin kullanıldığı biyolojik kontrol çalışmalarına katkıda bulunacaktır.
Fungal Pathogens Associated with Crown and Root Rot in Wheat-Growing Areas of Northern Kyrgyzstan
(Journal of Fungi, 2023) Derviş, Sibel; Derviş, Sibel
Abstract: Fungal species associated with crown and root rot diseases in wheat have been extensively studied in many parts of the world. However, no reports on the relative importance and distribution of pathogens associated with wheat crown and root rot in Kyrgyzstan have been published. Hence, fungal species associated with wheat crown/root rot were surveyed in three main wheat production regions in northern Kyrgyzstan. Fungal species were isolated on 1/5 strength potato-dextrose agar amended with streptomycin (0.1 g/L) and chloramphenicol (0.05 g/L). A total of 598 fungal isolates from symptomatic tissues were identified using morphological features of the cultures and conidia, as well as sequence analysis of the nuclear ribosomal internal transcribed spacer (ITS) region, the translation elongation factor 1α (TEF1), and the RNA polymerase II beta subunit (RPB2) genes. The percentage of fields from which each fungus was isolated and their relative percentage isolation levels were determined. Bipolaris sorokiniana, the causal agent of common root rot, was the most prevalent pathogenic species isolated, being isolated from 86.67% of the fields surveyed at a frequency of isolation of 40.64%. Fusarium spp. accounted for 53.01% of all isolates and consisted of 12 different species. The most common Fusarium species identified was Fusarium acuminatum, which was isolated from 70% of the sites surveyed with an isolation frequency of 21.57%, followed by Fusarium culmorum, Fusarium nygamai, Fusarium oxysporum, and Fusarium equiseti, all of which had a field incidence of more than 23%. Inoculation tests with 44 isolates representing 17 species on the susceptible Triticum aestivum cv. Seri 82 revealed that Fusarium pseudograminearum and F. culmorum isolates were equally the most virulent pathogens. The widespread distribution of moderately virulent B. sorokiniana appears to be a serious threat to wheat culture, limiting yield and quality. With the exception of F. culmorum, the remaining Fusarium species did not pose a significant threat to wheat production in the surveyed areas because common species, such as F. acuminatum, F. nygamai, F. oxysporum, and F. equiseti, were non-pathogenic but infrequent species, such as Fusarium redolens, Fusarium algeriense, and F. pseudograminearum, were highly or moderately virulent. Curvularia inaequalis, which was found in three different fields, was mildly virulent. The remaining Fusarium species, Fusarium solani, Fusarium proliferatum, Fusarium burgessii, and Fusarium tricinctum, as well as Microdochium bolleyi, Microdochium nivale, and Macrophomina phaseolina, were non-pathogenic and considered to be secondary colonizers. The implications of these findings are discussed.
Canker and leaf scorch on olive (Olea europaea L.) caused by Neoscytalidium dimidiatum in Turkey
(ScienceDirect, 2022) Derviş, Sibel; Özer, Göksel; Türkölmez, Şahimerdan; Derviş, Sibel
In a recent survey of olive groves in the Southeast Anatolia Region of Turkey, a new and serious decline of olive trees, beginning with foliar scorching and then dieback of twigs, branches, and even whole trees, was observed for the first time. In more advanced stages of the disease, necrosis and cankers were observed on the bark of the trunk, branches, and twigs. Isolations from symptomatic tissues from multiple cultivars in diverse locations yielded Neoscytalidium dimidiatum, which were identified using ITS, tef1, and tub2 loci of genomic DNA, in combination with morphological data. In vitro studies showed that conidial germination, hyphal growth, and pycnidia formation of the pathogen were positively correlated with elevated temperatures. Wild type olive “Delice”, “Edincik Su”, and “Memecik” seemed like the most resistant cultivars on which disease severity values were the lowest among 14 screened olive cultivars in two experimental orchards under natural conditions. Pathogenicity tests showed that “Gemlik” was the most susceptible cultivar showing the largest cankers and extensive scorch lesions. Isolates caused canker but not leaf scorch on O. europaea cultivars “Arbequina” “Halhalı”, “Manzanilla”, “Nizip Yağlık”, and “Saurani”. Neoscytalidium isolates are likely to have a negative impact on the health of diverse olive groves, which are primarily confined to Mediterranean-type climatic regions. These findings suggest an increased risk of infection in environments with increasing temperatures, as is common in the Southeast Anatolia Region of Turkey. The information gathered in this study will be used to examine the disease's epidemiology and establish disease control initiatives. This is also the first report of N. dimidiatum infecting O. europaea in the world.
A novel blight and root rot of chickpea: A new host for Neoscytalidium dimidiatum
(ScienceDirect, 2023) Derviş, Sibel; Bozoğlu, Tuba; Özer, Göksel; Derviş, Sibel
In the southeastern province of Mardin in Turkey, a severe and novel fungal infection affected all chickpea plant parts, resulting in blight symptoms on stem, petiole, branch, and leaf, defoliation, seed and root rot, and death. Neoscytalidium dimidiatum was identified as the agent responsible for this new blight and root rot using sequence analysis of the ITS, tef1, and tub2 loci as well as morphological data. The pathogen was found in all surveyed districts and fields, with varying incidences of blight and root rot, blight being nearly twice (40%) as common as root rot (21%), and root-rotted and blighted plants co-occurring in the majority of instances. All 92 N. dimidiatum isolates from various tissues induced necrotic lesions on the inoculated plants, indicating they were pathogenic for chickpea. Conidia exhibited germination across a temperature range, with increasing temperatures positively influencing germination rates, and mycelial growth was significantly influenced by temperature, with the optimal growth temperature observed to be 35 °C. The response of 25 Turkish chickpea cultivars and three other genotypes when subjected to inoculation with Ciar 12 and Ciar 78 isolates, representative of phylogenetic clusters, was evaluated based on the severity of blight and root rot. The majority of cultivars and genotypes displayed high susceptibility and suffered mortality when exposed to either soil or spray inoculation with each isolate. Among the assessed cultivars and genotypes, Çağatay had the lowest severity of root rot. This study is the first to report a natural infection of chickpea plants by N. dimidiatum. Under global warming, this may be detrimental to chickpea cultivation and habitat in southeastern Turkey, which is the origin of chickpeas.
Neoscytalidium dimidiatum: A newly identified postharvest pathogen of pears and its implications for pome fruits
(Wiley, 2024) Derviş, Sibel; Zholdoshbekova, Sezim; Guney, Inci Guler; Ozer, Goksel
T & uuml;rkiye is a prominent contributor to pear and diverse pome fruit production. Pear fruit with unusual brown to black spots and rot symptoms observed in public marketplaces in Mardin province have raised concerns regarding postharvest fruit health. The consistent isolation of a fungus from these fruits revealed morphological features indicative of Neoscytalidium dimidiatum. Phylogenetic confirmation of its identity ensued through BLASTn searches targeting, the internal transcribed spacer (ITS) of ribosomal DNA, the partial translation elongation factor 1-alpha gene (tef1), and the partial beta-tubulin gene (tub2). Pathogenicity evaluations were conducted on common pome fruits, namely pears, apples, and quinces, unveiling the susceptibility of all examined fruits to postharvest infection by this emergent pathogen. Furthermore, an investigation was carried out to discern the pathogen's response to varying temperature ranges on pear fruits, revealing that the most pronounced lesions occurred at 30 degrees C, followed by 25 degrees C, 35 degrees C, and 20 degrees C. Conversely, no lesion development was observed at 10 degrees C, 15 degrees C, or 40 degrees C. To the best of our knowledge, this study represents the first report of N. dimidiatum as the etiological agent responsible for postharvest rot in pear fruit. The implications of these findings highlight the potential threat posed by this pathogen to pome fruits postharvest, especially in regions where cold storage facilities are not widely utilized, warranting increased vigilance and preventive measures.
First report of fruit rot of eggplant caused by Pythium viniferum in Turkey
(SpringerLink, 2021) Derviş, Sibel; Özer, Göksel; Çiftçi, Osman; Derviş, Sibel
In August 2019, symptoms including dark brown and irregular sunken lesions or blights on the fruit pedicel and calyx of eggplants (Solanum melongena L.) occurred with a 3% incidence in two felds in Şanlıurfa province of Turkey.