Baran, Mehmet Fırat

Baran, Mehmet Fırat

Name Variants

Baran, M. F.
Baran, Mehmet Firat

Job Title

Doç. Dr.

Main Affiliation

Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü

Status

Former Staff

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Scholarly Output

52

Articles

50

Citation Count

0

Supervised Theses

1 Scholarly Output Search Results

Now showing 1 - 10 of 34

Biosynthesis, characterization, and investigation of antimicrobial and cytotoxic activities of silver nanoparticles using Solanum tuberosum peel aqueous extract
(Elsevier, 2023) Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Eftekhari, Aziz; Aytuğ Ava, Canan; İrtegün Kandemir, Sevgi; Cebe, Deniz Barış; Dağ, Beşir; Beilerli, Aferin; Khalilov, Rovshan
Metallic nanoparticle biosynthesis is thought to offer opportunities for a wide range of biological uses. The green process of turning biological waste into utilizable products gaining attention due to its economical and eco-friendly approach in recent years. This study reported the ability of Solanum tuberosum (ST) peel extract to the green synthesis of non-toxic, stable, small-sized silver nanoparticles without any toxic reducing agent utilizing the phytochemical components present in its structure. UV-visible spectroscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, flourier scanning electron microscopy, atomic force microscopy, transmission electron microscopy, and energy dispersive analysis X-ray confirmed the biosynthesis and char-acterization of silver nanoparticles. Also, dynamic light scattering and thermogravimetric ana-lyses showed stable synthesized nanoparticles. The antibacterial activity of the biosynthesized silver nanoparticles was evaluated against four different bacterial strains, Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus) Bacillus subtilis (B. subtilis), and a yeast, Candida albicans (C. albicans) using the minimum inhibitory concen-tration technique. The cytotoxic activities were determined against Human dermal fibroblast (HDF), glioblastoma (U118), colorectal adenocarcinoma (CaCo-2), and human ovarian (Skov-3) cell lines cancer cells using MTT test. The nanoparticle capping agents that could be involved in the reduction of silver ions to Ag NPs and their stabilization was identified using FTIR. Nano -particles were spherical in shape and had a size ranging from 3.91 to 27.07 nm, showed crys-talline nature, good stability (-31.3 mV), and the presence of capping agents. ST-Ag NPs significantly decreased the growth of bacterial strains after treatment. The in vitro analysis showed that the ST-Ag NPs demonstrated dose-dependent cytotoxicity against cell lines. Based on the data, it is feasible to infer that biogenic Ag NPs were capped with functional groups and demonstrated considerable potential as antibacterial and anticancer agents for biomedical and industrial applications.
The protective effects of different parts of hypericum perforatum extracts on human mononuclear leukocytes in hydrogen peroxide-induced DNA damage and their phenolic contents
(Medicine Science, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Baran, Mehmet Fırat
Oxidative stress is the state of the formation of some pathophysiological condition with the excessive increase of the normal amount of free radicals in the organism. In this study, the in vivo genotoxic and antigenotoxic effects of methanol and water extract and phenolic content of Hypericum perforatum flower, fruit, and seed methanol extracts were analyzed. HPLC was used to evaluate the quantities of 3,4-Dihydroxybenzoic acid, syringic acid, hydroxycinnamic acid, O-coumaric acid, caffeic acid, and catechin in the methanol extracts. The alkaline comet test was used to assess the DNA damage and protective effects of H. perforatum flower fruit, seed methanol, and water extract on human mononuclear leukocytes. The amounts of catechin and caffeic acid in seed methanol extract were found as quite high when compared to other extracts. The highest protective effects were seen at 10 and 50μg/ml concentrations of seed methanol extract. The optimum doses of fruit, flower, and seed extracts obtained from H. perforatum neutralized the genotoxic effect. This effect is stronger in seed methanol extract than other extracts. We suggest that more research is needed to evaluate the effects of H. perforatum phytochemicals in vitro and in vivo.
Fatty acid compositions of total lipid, phospholipid and triacylglycerol fractions of the wild edible mushroom pleurotus ostreatus and russula delica with cytotoxic activities on prostate carcinoma cell lines
(2019) Acay, Hilal; Baran, Mehmet Fırat
The aim of the study to investigate fatty acids (FAs) of Pleurotus ostreatus and Russula delica in Total Lipid (TL), Triacylglycerol (TG) and Phospholipid (PL) fractions. The major FAs of TL, TG, PL in both species were palmitic acid (PA), oleic acid (OLA), and linoleic acid (LA). In both species, total PUFA amounts were found to be higher than total monounsaturated fatty acids (MUFA) and total saturated fatty acids (SFA) in TL, TG and PL fractions. Also, insufficiently studied cytotoxic activity (using prostate carcinoma (PC-3) cell lines) of these mushrooms were investigated by using various solvent systems. Ethyl acetate extract of Pleurotus ostreatus and Russula delica showed significant inhibitory value at the concentrations of 520-530 μg/ml (99.45-92.82%) against PC-3 cell lines with the half-maximal inhibitory concentration (IC50); 274.53-297.77 μg/mL respectively. The present study is a guide for biochemical and nutritional values of both species and can be useful for further investigation on pharmacological applications.
Ecofriendly Synthesis of Silver Nanoparticles Using Ananas comosus Fruit Peels: Anticancer and Antimicrobial Activities
(Hindawi, 2021) Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Huseynova, Irada; Khalilov, Rovshan; Eftekhari, Aziz; Irtegun-Kandemir, Sevgi; Kavak, Deniz Evrim
Metallic nanoparticles are valuable materials and have a range of uses. Nanoparticles synthesized from plant wastes by environment-friendly methods have attracted the attention of researchers in recent years. Also, the advantages of biological resources and synthesis methods are attracting attention. In this study, silver nanoparticles were synthesized from Ananas comosus fruit peels using ecofriendly method steps. The characterization of the particles obtained was determined by using a UV-visible spectrophotometer (UV-Vis.), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction diffractometer (XRD), Fourier scanning electron microscope (FESEM), and transmission electron microscopy (TEM). The nanoparticles showed maximum absorbance at 463 nm, measuring 11.61 in crystal nanosize, and presented spherical in appearance. An antimicrobial activity test was determined with the minimum inhibition concentration (MIC) method. The nanoparticles showed promising inhibitory activity on the Gram-positive and Gram-negative pathogen microorganisms (Escherichia coli ATCC25922, Staphylococcus aureus ATCC29213, Bacillus subtilis ATCC11774, Pseudomonas aeruginosa ATCC27833 bacteria, and Candida albicans yeast) at low concentrations. The cytotoxic and growth inhibitory effects of silver nanoparticles on different cancer cell lines were examined via the MTT assay. © 2021 Ayşe Baran et al.
Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications
(Taylor & Francis Online, 2021) Keskin, Cumali; Aktepe, Necmettin; Baran, Ayşe; Baran, Mehmet Fırat; Aktepe, Necmettin; Yavuz, Ömer; İrtegun Kandemir, Sevgi
In this study, silver nanoparticles (AgNPs) were synthesized economically and simply using an environmentally friendly method with the extract obtained from agricultural waste olive leaves. AgNPs synthesized according to the analysis data were determined to have maximum absorbance at 433.5 nm wavelength, spherical appearance, 7.2 nm crystal nano size and -19.9 mV zeta potential. It was determined by the microdilution method with Minimum Inhibition Concentration (MIC) that AgNPs exert a suppressive effect on the growth of pathogen gram-negative, positive bacteria and yeast at very low concentrations. The cytotoxic effects of the particles were investigated on healthy cell lines (HDF) and cancerous cell lines (U118, CaCo-2, Skov-3). AgNPs showed up to 70% suppression in cancer cell lines.
Green synthesis of silver nanoparticles mediated Diospyros kaki L. (Persimmon): determination of chemical composition and evaluation of their antimicrobials and anticancer activities
(Frontiers in Chemistry, 2023) Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Baran, Mehmet Fırat; Eftekhari, Aziz; Gareev, Ilgiz
The eco-friendly synthesis of metallic nanoparticles (MNPs) using biological materials is an encouraging and innovativeness approach to nanotechnology. Among other synthesizing methods, biological methods are chosen because of their high efficiency and purity in many aspects. In this work, using the aqueous extract obtained from the green leaves of the D. kaki L. (DK); silver nanoparticles were synthesized in a short time and simply with an eco-friendly approach. The properties of the synthesized silver nanoparticles (AgNPs) were characterized using various techniques and measurements. In the characterization data of AgNPs, Maximum absorbance at 453.34 nm wavelengths, the average size distribution of 27.12 nm, the surface charge of -22.4 mV, and spherical appearance were observed. LC-ESI-MS/MS analysis was used to assess the compound composition of D. kaki leaf extract. The chemical profiling of the crude extract of D. kaki leaves revealed the presence of a variety of phytochemicals, predominantly phenolics, resulting in the identification of five major high-feature compounds: two major phenolic acids (Chlorogenic acid and Cynarin), and tree flavonol glucosides (hyperoside, quercetin-3-glucoside, and quercetin-3- D-xyloside). The components with the highest concentrations were cynarin, chlorogenic acid, quercetin-3- D-xyloside, hyperoside, and quercetin-3-glucoside, respectively. Antimicrobial results were determined by a MIC assay. The biosynthesized AgNPs exhibited strong antibacterial activity against the human and food pathogen Gram (+ and -) bacteria and good antifungal activity against pathogenic yeast. It was determined that 0.03-0.050 μg/mL concentrations ranges of DK-AgNPs were growth suppressive concentrations on all pathogen microorganisms. The MTT technique was used to study the cytotoxic effects of produced AgNPs on cancer cell lines (Glioblastoma (U118), Human Colorectal Adenocarcinoma (Caco-2), Human Ovarian Sarcoma (Skov-3) cancer cell lines, and Human Dermal Fibroblast (HDF) healthy cell line). It has been observed that they have a suppressive effect on the proliferation of cancerous cell lines. After 48 h of treatment with Ag-NPs, the DK-AgNPs were found to be extremely cytotoxic to the CaCo-2 cell line, inhibiting cell viability by up to 59.49% at a concentration of 50 g mL-1. It was found that the viability was inversely related to the DK-AgNP concentration. The biosynthesized AgNPs had dose-dependent anticancer efficacy. Because of the high concentration of bioactive chemicals in Diospyros kaki, it may be employed as a biological resource in medicinal applications. DK-AgNPs were shown to be an effective antibacterial agent as well as a prospective anticancer agent. The results provide a potential approach for the biogenic production of DK-AgNPs utilizing D. kaki aqueous leaf extract.
Hypericum Triquetrifolium Turra Bitkisinden Gümüş Nanopartiküllerin Sentezi, Karekterizasyonu ve Antimikrobial Etkinliğinin İncelenmesi
(2019) Umaz, Adil; Keskin, Cumali; Baran, Mehmet Fırat; Koç, Adil; Keskin, Cumali
Bu çalışmada, biyolojik yöntemle Hypericum triquetrifolium Turra bitkisi kullanılarak gümüşnanopartiküller (AgNP) sentezlendi. Sentezlenen gümüş nanopartiküller (AgNP) Fourier Transformİnfrared Spektroskopisi (FT-IR), Ultraviyole/Görünür Işık Absorpsiyon Spektrofotometresi (UV-vis),X-Işını Kırınımı (XRD), Taramalı Elektron Mikroskobu (SEM), Enerji Dağılımlı X-ışını (EDAX) veTermogravimetrik-Difarensiyel Termal Analiz (TGA-DTA) gibi spektroskopik yöntemler kullanılarakaydınlatıldı. Sentezlenen gümüş nanopartiküllerin mikroorganizmalar üzerindeki antimikrobiyalaktiviteleri incelenmiştir.
Kinetic and isotherm investigation into the removal of heavy metals using a fungal-extract-based bio-nanosorbent
(Environmental Technology and Innovation, 2020) Yıldırım, Ayfer; Acay, Hilal; Baran, Mehmet Fırat
Adsorption is very economical and environmentally friendly method that is commonly accepted as a promising technique for the removal of heavy metals. In this study a fungal-extract-based (FE-CB) bio-nanosorbent was prepared and used as an efficient biosorbent for the removal of heavy metals, namely Cu(II) and Ni(II), from aqueous solutions. FE-CB was characterized by scanning electron microscope, Brunauer–Emmett–Teller surface area and porosity analyzer, Fourier transform infrared, x-ray diffraction, differential scanning calorimeter, thermalgravimetric analysis and zeta potential. The Brunauer–Emmett–Teller surface area, pore volume and average pore diameter of FE-CB were 7.43 m2/g, 0.060 cm3/g, and 2.82 nm, respectively. The adsorbtion properties of FE-CB onto both Cu(II) and Ni(II) were investigated in terms of biosorbent dosage, temperature, initial concentration of Cu(II) and Ni(II) ions, pH and contact time in the batch experiments. The dependence of the biosorption mechanism on pH was revealed and the optimum pH was determined as 6 for Ni(II) and 5 for Cu(II). The Langmuir and Freundlich isothermal models and the kinetic Pseudo-first-order and Pseudo-second-order kinetic models were used to describe the adsorption performance of FE-CB. The activation energy was calculated by pseudo-second-order rate constants. In addition, thermodynamic parameters, standard Gibbs free energy, standard enthalpy and standard entropy were analyzed using the (Van't Hoff equation). The biosorption process was found to be spontaneous, favorable and endothermic.
Evaluation and characterization of Pleurotus eryngii extract-loaded chitosan nanoparticles as antimicrobial agents against some human pathogens
(Taylor & Francis Online, 2020) Acay, Hilal; Yıldırım, Ayfer; Erdem Güzel, Elif; Baran, Mehmet Fırat; Baran, Mehmet Fırat
With the increase of antibiotic resistance, which is present at a worrying rate, research on the use of newly developed nanoparticles as an antimicrobial agent with green biotechnology has intensified. The study aimed to investigate the antimicrobial effects of chitosan nanoparticles (CSNP) synthesized using Pleurotus eryngii extract (PE). Characterization of P. eryngii-loaded chitosan nanoparticles (PE-CSNPs) was performed with Fourier transform infrared spectrophotometer, X-ray diffraction, Field-emission scanning electron microscopy, Brunauer-Emmett-Teller, Differential scanning calorimetry, and zeta potential techniques. The FE-SEM images showed that the surface morphology of nanoparticles is similar to CS, but has more porosity network and smaller dimensions structure. The average particle size of spherical PE-CSNPs was obtained as 330.1 nm. The specific surface area and average pore diameter of the synthesized nanoparticles were found as 3.99 m2g-1 and 2.25 nm, respectively. X-ray diffraction determines the presence of an amorphous peak at 2θ = 21.2° results from CS and PE. PE-CSNPs synthesized using P. eryngii extract showed strong antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Candida albicans as 0.0156, 0.0625, 0.0625 and 0.0312 mg ml-1, respectively. Thus, it was determined that chitosan nanoparticles formed by the green synthesis of P. eryngii extract showed strong anti-microbial properties.
Farklı Biyosorbentler Kullanarak Su Ortamında Ağır Metallerin Biyosorbsiyon Metodu ile Giderilmesi
(2022) Keskin, Cumali; Aktepe, Necmettin; Baran, Mehmet Fırat; Baran, Ayşe; Baran, Ayşe
Su canlılar için ekosistemin önemli parçasıdır. Endüstrileşme ile birlikte su kaynaklarındaki kirlenme endişe edilir boyutlara ulaşmıştır. Ağır metallere bağlı su kirliliği ve artan konsantrasyonları su ekosistemlerine verdiği zarar nedeniyle araştırmacıların konuya olan ilgisinin artmasına sebep olmuştur. Ağır metallerin sularda oluşturduğu kirliliği gidermek ciddi maliyet ve zaman gerektirmektedir. Son yıllarda suda bulunan ağır metallerin uzaklaştırılması için bakteriler kullanılarak biyosorbsiyon yönteminin kullanılması yaygınlaşmıştır. Bu yöntemin tercih edilmesinin temel sebebi gram pozitif bakterilerin hücre duvarında kalın bir peptidoglikan tabakasına sahip olması ile adsorbsiyon kapasitesini artırmasıdır. Bu çalışmada içme, atık, nehir suları ve suni olarak hazırlanan numunelerde çalkalamalı metod kullanarak ağır metal biyosorbsiyonu ile birlikte çoklu hazırlanmış ağır metal çözeltilerinde biyosorbsiyon rekabeti incelenmiştir. Bu işlemler için Dicle nehri bölgesine ait topraklardan izole edilen Bacillus licheniformis sp. Bacillus subtilis sp. ve Bacillus subtilis (ATCC 6051) suşları ile sulu çözeltiden Cd (II), Cu (II), Pb (II), Fe (II), Ni (II) ve Zn (II) metallerinin biyosorbsiyonu değerlendirildi. B. subtilis suşları ve B.licheniformis sp. organizmalarının yüzey morfoljik yapıları SEM görüntüleri, element kompozisyonları EDAX verileri ile incelendi. ICP-OES kullanılarak element içeriği tespit edildi. Sulu çözeltideki Cd (II), Cu (II), Pb (II), Fe (II), Ni (II) ve Zn (II) metal iyonları farklı pH’larda 25 °C de 0,25 mg L-1 biomass ile biyosorbsiyon gerçekleştirildi. pH 6.0 da maksimum biyosorbsiyon ile metal iyonlarının % 98 varan oranda giderildiği belirlendi.