Aktepe, Necmettin
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Aktepe, N.
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Doçent
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necmettinaktepe@gmail.com
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Department of Nursing / Hemşirelik Bölümü
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1
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8
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0
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7
AFFORDABLE AND CLEAN ENERGY
0
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17
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13
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2
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4
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3
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8
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9
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1
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16
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5
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12
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10
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0
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14
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2
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11
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2
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15
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0
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6
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2
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11
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115
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6
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21
Citations
243
Scholarly Output
24
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20
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3/0
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2
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0
WoS Citation Count
114
Scopus Citation Count
115
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6
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6
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0
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6
WoS Citations per Publication
4.75
Scopus Citations per Publication
4.79
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15
Supervised Theses
2
| Journal | Count |
|---|---|
| International Journal of Agriculture, Environment and Food Sciences | 4 |
| Medicine Science | 2 |
| Progress in Nutrition | 2 |
| Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi | 2 |
| Chemical Papers | 1 |
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Now showing 1 - 10 of 24
Article Synthesis, Characterization and Evaluation of Antimicrobial Activities of Silver Nanoparticles Obtained From Rumex Acetosella L. (sorrel) Plant(2022) Aktepe, Necmettin; Bütüner, Hafize; Baran, Ayşe; Keskin, Cumali; Baran, Mehmet FıratRumex acetosella L. (sorrel) is a plant belonging to the Polygonaceous family and is a species that grows naturally across Turkey. In this study, the characterization of silver nanoparticles (AgNPs) obtained from the Rumex acetocella L. (RA) plant using the green synthesis method was performed and their antimicrobial activities were investigated. AgNPs were successfully synthesized in the first stage of the study using plant extract taken from plant samples collected from the natural growing environment. Characterization of synthesized AgNPs was performed using appropriate analytical methods (UV-vis, FT-IR, XRD, SEM-EDX, TEM, Zeta Potential and Zeta Sizer). According to the analysis results, it was determined that AgNPs had a maximum absorbance at 476 nm wavelength, a pentagonal, hexagonal, and spherical appearance, a size of 29.16 nm, and a zeta potential of -9.88 mV. The antimicrobial activities of AgNPs were tested using the microdilution technique, in which Minimum Inhibition Concentration (MIC) values were determined on gram-positive Staphylococcus aureus, Bacillus subtilis and gram-negative Pseudomonas aeruginosa, Escherichia coli bacteria and Candida albicans fungus. It showed a very strong antimicrobial effect on C. albicans, S. aureus and P. aeruginosa. Consequently, AgNPs had stronger antimicrobial activity at low concentrations and when compared to commercial antibiotics.Master Thesis Rumex acetosella l. (kuzukulağı) bitkisinden gümüş nanopartiküllerin sentezi, karakterizasyonu ve antimikrobiyal aktivitelerinin değerlendirilmesi(Mardin Artuklu Üniversitesi, 2022) Bütüner, Hafize; Aktepe, NecmettinRumex acetosella L. (kuzukulağı), Polygonaceae familyasına mensup bir bitki olup ülkemiz genelinde doğal olarak yetişen bir türdür. Bu tez çalışmasında Rumx acetosella L. bitkisinden yeşil sentez yöntemi ile elde edilen gümüş nanopartiküllerin (AgNP) karakterizasyonu ve antimikrobiyal aktiviteleri incelenmiştir. Araştırmanın ilk aşamasında doğal yetiştiği ortamından toplanan bitki örneklerinden elde edilen bitki özütü ile AgNP'ler başarılı bir şekilde sentezlenmiştir. Elde edilen Ra-AgNP'lerin karakterizasyonu uygun analiz yöntemleri kullanılarak (UV-vis, FT-IR, XRD, SEM-EDX, TEM, Zeta Potansiyeli ve Zeta Sizer) tespit edilmiştir. Analiz sonuçlarına göre AgNP'lerin 476 nm dalga boyunda maksimum absorbans gösterdiği; beşgen, altıgen ve küresel görünüm; 29.16 nm boyut; -¬9.88 mV zeta potansiyeline sahip oldukları belirlenmiştir. AgNP'lerin antimikrobiyal aktiviteleri gram pozitif Staphylococcus aureus, Bacillus subtilis ve gram negatif Pseudomonas aeruginosa, Escherichia coli bakterileri ile Candida albicans mantarı üzerinde MİK (Minimum İnhibisyon Konsantrasyonu) değerlerinin belirlendiği mikrodilüsyon yöntemi ile tespit edilmiştir. Sonuç olarak Ra-AgNP'lerin hem düşük konsantrasyonlarda hem de ticari antibiyotiklere kıyasla daha güçlü antimikrobiyal aktivite gösterdiği belirlenmiştir.Article Citation - WoS: 6Citation - Scopus: 4Biosynthesis of AgNPs by extract from waste leaves of Citrullus lanatus sp. (watermelon); characterization, antibacterial and antifungal effects(Progress in Nutrition, 2021) Aktepe, Necmettin; Baran, AyşeSilver nanoparticles (AgNPs) are valuable materials with a large number of sectors used. Green synthesis is very important for biomedical applications as they show biocompatible properties. In this study, AgNPs were easily synthesized using the environmentally friendly green synthesis approach using agricultural waste parts of Citrullus lanatus sp. plant grown in Diyarbakır region. Characterization of synthesized AgNPs was made. Fourier Transform Infrared Spectroscopy (FTIR) analysis was used to evaluate the phytochemicals responsible for effective reduction in the formation of AgNPs. UV-visible spectrophotometer (UV-Vis.) Spectra were also used to determine the presence of AgNPs. X-Ray Diffraction Diffractometer (XRD), Scanning Electron Microscope (SEM), Transmission Electron Microscope, Zeta potential analyzes were performed to define the crystal structures, dimensions and surface charges of AgNPs, respectively. In these data, it was determined that AgNPs showed maximum absorbance at 460 nm, spherical appearance, 21.27 crystal nano size and -30.05 mV zeta potential. Antimicrobial effects of AgNPs on gram positive Staphylococcus aureus (S. aureus) and Bacillus subtilis (B. subtilis) bacteria, gram negative Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) bacteria, as well as on fungus C. albicans pathogenic microorganisms It was analyzed by specifying the Minimum Inhibition Concentration (MIC) by microdilution.Master Thesis Cyclotrichium origanifolium L. bitkisinden gümüş nanopartiküllerin sentezi, karakterizasyonu ve antimikrobiyal aktivitelerinin değerlendirilmesi(Mardin Artuklu Üniversitesi, 2021) Erbay, Nazım; Aktepe, NecmettinCyclotrichium origanifolium, Lamiaceae familyasaına ait bir bitki olup, ülkemizde Batı ve Güney Anadolu bölgelerinde yetişen bir türdür. Bu tez çalışmasında Cyclotrichium origanifolium bitkisinden biyolojik yolla elde edilen gümüş nanopartiküllerin (AgNP) antimikrobiyal aktiviteleri incelendi. Araştırmanın ilk bölümünde doğal ortamından toplanan bitki örneklerinden elde edilen özütten AgNP'ler başarılı bir şekilde üretildi. Elde edilen AgNP'lerin karakterizasyon işlemleri uygun spektral analiz yöntemleri; Uv-Vis, FT-IR, SEM-EDX, XRD ile belirlendi. Analiz sonuçlarına göre AgNP'lerin küresel şekilli ve ortalama 17,60 nm çapında olduğu tespit edildi. AgNP'lerin antimikrobiyal etkisi MİK yöntemi ile belirlendi. Test mikroorganizmaları olarak gram pozitif; S. aureus, B. subtilis ve gram negatif; E. coli, P. aeruginosa bakterileri ile C. albicans fungal patojen türleri kullanıldı. Mikroorganizma gelişimlerinin baskılanması, çalışmamızda kullanılan standart antibiyotikler ile yeşil sentez yöntemle üretilen AgNP'lerin etkinlikleri karşılaştırılarak incelendi. Sonuç olarak Cyclotrichium origanifolium bitkisinden üretilen AgNP'lerin antimikrobiyal aktivitesi, standart antibiyotiklere göre daha güçlü etki gösterdiği gözlendi.Article Investigation of the Effect of Cuscuta Spp. Extract on Cell Viability(2025) Öziç, Cem; Baran, Ayşe; Aktepe, Necmettin; Güneş, Zübeyir; Baran, Mehmet FiratCancer is becoming an increasingly serious health problem worldwide. While various treatment approaches exist for diseases like colorectal cancer, they have not yet been found to be fully effective. Therefore, the development of new anti-cancer drugs is crucial. Cuscuta is a plant used in functional foods and for traditional medicinal purposes in various cultures, including Ayurveda and its medicinal traditions, which are widespread throughout the world. In preclinical studies, Cuscuta spp. have demonstrated anticytotoxic and cytoprotective properties and potential anticancer effects, particularly in various cell lines and animal models. Fibroblasts are scientifically proven to be the primary normal cell type of connective tissue, typically mesenchymal in origin. This is represented by the laboratory L929 mouse fibroblast cell line. Colorectal cancer is the third most common cancer worldwide and the third most common cause of cancer-related death. Our study aimed to determine the cytotoxic effects of Cuscuta spp extract, known to have medicinal effects in various diseases in public health, on the L929 mouse fibroblast cell line and the HT-29 human colorectal adenocarcinoma cell line. For cytotoxic analysis, the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] enzymatic test was preferred. In vitro testing using Cuscuta spp extract revealed decreased viability in L929 fibroblast cell line and HT-29 human colorectal adenocarcinoma cells exposed to Cuscuta spp plant extract for 24 and 48 hours. The plant extract showed more cytotoxic effects on fibroblast cells, a normal cell line. In conclusion, this study demonstrates that Cuscuta spp. extract exhibits high cytotoxicity on normal cells and has limited effects on cancer cells. Further studies are needed for its pharmacological use.Article Citation - WoS: 1Removal of Heavy Metals in Water by Biosorption Method Using Three Different Bacillus sp-derived Biosorbents(Kahramanmaraş Sütçü İmam Üniversitesi, 2022) Baran, Mehmet Fırat; Düz, Zahir; Baran, Ayşe; Keskin, Cumali; Aktepe, NecmettinWater is an important part of the ecosystem for life. With industrialization, pollution in water resources has reached a worrying level. Water pollution due to heavy metals and their increasing concentrations have caused researchers to increase their interest in the subject due to the damage they cause to water ecosystems. It requires serious cost and time to eliminate the pollution caused by heavy metals in water. In recent, years, the use of biosorption method using bacteria to remove heavy metals in water has become widespread. The main reason why this method is preferred is that gram-positive bacteria have a thick peptidoglycan layer on the cell wall and increases the adsorption capacity. In this study. in drinking, waste, river water and artificially prepared samples, batch method of heavy metal biosorption and biosorption competition in multiple prepared heavy metal solutions were investigated. For these processes. Bacillus licheniformth sp. Bacillus subtilis sp. and Bacillus subtilis (ATCC 6051) strains were used as a biosorbent. Biosorbtion of Cd (II), Cu (H), Pb (II), Fe(II), Ni (II) and Zn (II) metals from waters with these biosorbents at different pHs at. 25 degrees C with 0.25 mg LI It was investigated. Surface morphological structures of biosorbents were evaluated using SEM images and element compositions were evaluated using EDAX profile. Element content was determined using ICP-OES. It was found that heavy metal ions were removed up to 98% with maximum biosorption at pH 6.0.Article Synthesis, Characterization, and Evaluation of the Antimicrobial Activities of Silver Nanoparticles From Cyclotrichium Origanifolium L.(2022) Baran, M. Fırat; Keskin, Cumali; Erbay, Nazım; Aktepe, Necmettin; Baran, AyşeCyclotricium origanifolium is a plant belonging to the Lamiaceae family and is a species that grows in the Western and Southern Anatolian regions of Turkey. In our study, the antimicrobial activities of silver nanoparticles (AgNP) were investigated through Cyclotricium origanifolium plant extract. Characterization processes of the obtained AgNPs, suitable spectral analysis methods; Uv-Vis was determined by FT-IR, SEM-EDX, XRD. According to the results of the analysis, it was determined that the AgNPs were spherical in shape and had an average diameter of 17.60 nm. The antimicrobial effect of AgNPs was determined by the minimum inhibition concentration (MIC) method. Gram positive as test microorganisms; Staphylococcus aureus, Bacillus subtilis and gram negative; Escherichia coli, Pseudomonas aeruginosa bacteria, and Candida albicans fungal pathogen species were used. The suppression of microorganism growth was investigated by comparing the efficacy of standard antibiotics used in our study with AgNPs produced by the green synthesis method. It has been observed that the obtained AgNPs have a very strong effect on gram-positive B. subtilis and gram-negative E. coli bacteria, and are more effective against C. albicans than the normal antifungal drug. It was determined that the antimicrobial activity of AgNPs produced from C. origanifolium L. plants showed a stronger effect than standard antibiotics.Article The protective effects of different parts of hypericum perforatum extracts on human mononuclear leukocytes in hydrogen peroxide-induced DNA damage and their phenolic contents(Medicine Science, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Atalar, Mehmet Nuri; Baran, Mehmet FıratOxidative stress is the state of the formation of some pathophysiological condition with the excessive increase of the normal amount of free radicals in the organism. In this study, the in vivo genotoxic and antigenotoxic effects of methanol and water extract and phenolic content of Hypericum perforatum flower, fruit, and seed methanol extracts were analyzed. HPLC was used to evaluate the quantities of 3,4-Dihydroxybenzoic acid, syringic acid, hydroxycinnamic acid, O-coumaric acid, caffeic acid, and catechin in the methanol extracts. The alkaline comet test was used to assess the DNA damage and protective effects of H. perforatum flower fruit, seed methanol, and water extract on human mononuclear leukocytes. The amounts of catechin and caffeic acid in seed methanol extract were found as quite high when compared to other extracts. The highest protective effects were seen at 10 and 50μg/ml concentrations of seed methanol extract. The optimum doses of fruit, flower, and seed extracts obtained from H. perforatum neutralized the genotoxic effect. This effect is stronger in seed methanol extract than other extracts. We suggest that more research is needed to evaluate the effects of H. perforatum phytochemicals in vitro and in vivo.Article Citation - WoS: 8Citation - Scopus: 8Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium(Wiley Online Library, 2022) Aktepe, Necmettin; Baran, Ayşe; Atalar, Mehmet Nuri; Baran, Mehmet Fırat; Keskin, Cumali; Taşkin, Abdullah; Yavuz, Ömer; Demirtaş, İbrahim; Oğuz, Ercan; Jahan, IsratCyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography–electrospray ionization– tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.Article Green synthesis and antimicrobial effects of silver nanoparticles by pumpkin cucurbita maxima fruit fiber(Medicine Science, 2022) Aktepe, Necmettin; Baran, AyşeIn the present study, for the first time, green synthesis of silver nanoparticles was carried out with the extract obtained from the fibrous structure in the pumpkin Cucurbita maxima (CM) fruit. The presence of silver nanoparticles formed by the reduction of Ag+ ions was determined with the maximum absorbance values of 442.89 nm in wavelength scans made by UV-Vis spectroscopy analysis. The functional groups of the phytochemicals involved in the reduction were examined by Fourier-transform infrared (FT-IR) spectroscopy. For the morphology of the synthesized AgNPs, they were found to be uniform in spherical appearance using atomic power microscopy (AFM), transmission electron microscopy (TEM), and field emission scanning electron microscopy (FE-SEM) images. The surface charges of AgNPs were determined to be -16.4 mV by Zeta potential analysis. Their crystal structures and nano-sizes were evaluated by X-ray diffraction (XRD) data to be in cubic pattern, and their size was 21.20 nm using the Debye-Scherrer equation. AgNPs (CM-AgNPs) synthesized via CM provided growth suppression at concentrations of 1.00-0.12, 2.00-1.00, and 0.50 µg/mL on pathogen gram-positive, gram-negative strains and the fungus Candida albicans, respectively. These concentrations were effective against antibiotics and silver solution at a very low concentration.
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