Aktepe, Necmettin

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Profile URL
https://hdl.handle.net/20.500.12514/6984
Name Variants
Aktepe, N.
Job Title
Doçent
Email Address
necmettinaktepe@gmail.com
Main Affiliation
Department of Nursing / Hemşirelik Bölümü
Status
Website
ORCID ID
0000-0003-2192-9049
Scopus Author ID
56556803800
Turkish CoHE Profile ID
101564
Google Scholar ID
x74F1TIAAAAJ
WoS Researcher ID
ABA-4810-2020

Sustainable Development Goals

NO POVERTY1
NO POVERTY
0
Research Products
ZERO HUNGER2
ZERO HUNGER
0
Research Products
GOOD HEALTH AND WELL-BEING3
GOOD HEALTH AND WELL-BEING
8
Research Products
QUALITY EDUCATION4
QUALITY EDUCATION
0
Research Products
GENDER EQUALITY5
GENDER EQUALITY
0
Research Products
CLEAN WATER AND SANITATION6
CLEAN WATER AND SANITATION
2
Research Products
AFFORDABLE AND CLEAN ENERGY7
AFFORDABLE AND CLEAN ENERGY
0
Research Products
DECENT WORK AND ECONOMIC GROWTH8
DECENT WORK AND ECONOMIC GROWTH
0
Research Products
INDUSTRY, INNOVATION AND INFRASTRUCTURE9
INDUSTRY, INNOVATION AND INFRASTRUCTURE
1
Research Products
REDUCED INEQUALITIES10
REDUCED INEQUALITIES
0
Research Products
SUSTAINABLE CITIES AND COMMUNITIES11
SUSTAINABLE CITIES AND COMMUNITIES
2
Research Products
RESPONSIBLE CONSUMPTION AND PRODUCTION12
RESPONSIBLE CONSUMPTION AND PRODUCTION
0
Research Products
CLIMATE ACTION13
CLIMATE ACTION
0
Research Products
LIFE BELOW WATER14
LIFE BELOW WATER
2
Research Products
LIFE ON LAND15
LIFE ON LAND
0
Research Products
PEACE, JUSTICE AND STRONG INSTITUTIONS16
PEACE, JUSTICE AND STRONG INSTITUTIONS
0
Research Products
PARTNERSHIPS FOR THE GOALS17
PARTNERSHIPS FOR THE GOALS
0
Research Products
Documents

11

Citations

115

h-index

6

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Documents

21

Citations

243

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Scholarly Output

25

Articles

20

Views / Downloads

85/182

Supervised MSc Theses

2

Supervised PhD Theses

0

WoS Citation Count

114

Scopus Citation Count

115

Patents

0

Projects

6

WoS Citations per Publication

4.56

Scopus Citations per Publication

4.60

Open Access Source

15

Supervised Theses

2

JournalCount
International Journal of Agriculture, Environment and Food Sciences4
Medicine Science2
Progress in Nutrition2
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi2
Chemical Biology and Drug Design1
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Now showing 1 - 10 of 25
  • Thumbnail Image
    Book Part
    Effects of Essential Oil-Based Nanoparticles on the Plant Defense System
    (Elsevier, 2026) Yıldıztekin, Mahmut; Baran, Mehmet Fırat; Güneş, Zübeyir; Aktepe, Necmettin; Baran, Ayşe
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    Article
    Investigation of the Effect of Cuscuta Spp. Extract on Cell Viability
    (2025) Öziç, Cem; Baran, Ayşe; Aktepe, Necmettin; Güneş, Zübeyir; Baran, Mehmet Firat
    Cancer is becoming an increasingly serious health problem worldwide. While various treatment approaches exist for diseases like colorectal cancer, they have not yet been found to be fully effective. Therefore, the development of new anti-cancer drugs is crucial. Cuscuta is a plant used in functional foods and for traditional medicinal purposes in various cultures, including Ayurveda and its medicinal traditions, which are widespread throughout the world. In preclinical studies, Cuscuta spp. have demonstrated anticytotoxic and cytoprotective properties and potential anticancer effects, particularly in various cell lines and animal models. Fibroblasts are scientifically proven to be the primary normal cell type of connective tissue, typically mesenchymal in origin. This is represented by the laboratory L929 mouse fibroblast cell line. Colorectal cancer is the third most common cancer worldwide and the third most common cause of cancer-related death. Our study aimed to determine the cytotoxic effects of Cuscuta spp extract, known to have medicinal effects in various diseases in public health, on the L929 mouse fibroblast cell line and the HT-29 human colorectal adenocarcinoma cell line. For cytotoxic analysis, the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] enzymatic test was preferred. In vitro testing using Cuscuta spp extract revealed decreased viability in L929 fibroblast cell line and HT-29 human colorectal adenocarcinoma cells exposed to Cuscuta spp plant extract for 24 and 48 hours. The plant extract showed more cytotoxic effects on fibroblast cells, a normal cell line. In conclusion, this study demonstrates that Cuscuta spp. extract exhibits high cytotoxicity on normal cells and has limited effects on cancer cells. Further studies are needed for its pharmacological use.
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    Article
    Citation - WoS: 3
    Citation - Scopus: 6
    Anticancer, Antioxidant and Antimicrobial Activity of Elaeagnus Angustifolia L. Leaf Extract
    (Dove Medical Press Ltd, 2025) Do Gan, Serap; Mese, Ahmet; Baran, Mehmet Firat; Baran, Ayse; Aktepe, Necmettin; Ahmadian, Elham; Aktas, Husnu; Doğan, Serap
    Objective: This study was conducted to determine and compare the antioxidant, cytotoxic, and antimicrobial effects of spindle leaves of Elaeagnus angustifolia L. (E. angustifolia) (oleaster) leaves. Methods: Total phenolic content was measured using the Folin-Ciocalteu method, phenolic compound analysis by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) and antimicrobial effect by the minimum inhibition concentration (MIC) method. The free radical scavenging activity was determined by the (2,2-Diphenyl-1-picrylhydrazyl) DPPH method, the free radical scavenging activity was determined by the ABTS method, and cytotoxicity assays were performed by the MTT method in human retinal epithelium cells (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer cells (DU-145) cell lines. Results: High amounts of gallic acid, protocatechuic acid, and o-coumaric acid were identified as phenolic compounds. E. angustifolia was found to have a good antioxidant capacity and high free radical scavenging capacity. In this study, for the first time, E. angustifolia leaf extract was used to investigate cytotoxic effects on human retinal epithelium (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer (DU-145) cells and antimicrobial effects on Listeria monocytogenes American Type Culture Collection (ATTC) 7644, Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 11774, and Candida albicans ATCC 10231 microorganisms. The highest cytotoxic effect was observed in the DU-145 cell line, and the highest antimicrobial effect was observed in Listeria monocytogenes ATTC 7644 and Candida albicans ATCC 10231. The leaf extract of the plant contains some important phenolic compounds and has high free radical scavenging capacity, a good anticancer effect, and effective antimicrobial activity on yeast species such as C. albicans. Conclusion: Our study will contribute greatly to the search for anticancer and antimicrobial agents, especially from a pharmacological perspective, by examining biological activity using three different methods.
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    Article
    The protective effects of different parts of hypericum perforatum extracts on human mononuclear leukocytes in hydrogen peroxide-induced DNA damage and their phenolic contents
    (Medicine Science, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Atalar, Mehmet Nuri; Baran, Mehmet Fırat
    Oxidative stress is the state of the formation of some pathophysiological condition with the excessive increase of the normal amount of free radicals in the organism. In this study, the in vivo genotoxic and antigenotoxic effects of methanol and water extract and phenolic content of Hypericum perforatum flower, fruit, and seed methanol extracts were analyzed. HPLC was used to evaluate the quantities of 3,4-Dihydroxybenzoic acid, syringic acid, hydroxycinnamic acid, O-coumaric acid, caffeic acid, and catechin in the methanol extracts. The alkaline comet test was used to assess the DNA damage and protective effects of H. perforatum flower fruit, seed methanol, and water extract on human mononuclear leukocytes. The amounts of catechin and caffeic acid in seed methanol extract were found as quite high when compared to other extracts. The highest protective effects were seen at 10 and 50μg/ml concentrations of seed methanol extract. The optimum doses of fruit, flower, and seed extracts obtained from H. perforatum neutralized the genotoxic effect. This effect is stronger in seed methanol extract than other extracts. We suggest that more research is needed to evaluate the effects of H. perforatum phytochemicals in vitro and in vivo.
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    Article
    Citation - WoS: 8
    Citation - Scopus: 8
    Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
    (Wiley Online Library, 2022) Aktepe, Necmettin; Baran, Ayşe; Atalar, Mehmet Nuri; Baran, Mehmet Fırat; Keskin, Cumali; Taşkin, Abdullah; Yavuz, Ömer; Demirtaş, İbrahim; Oğuz, Ercan; Jahan, Israt
    Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography–electrospray ionization– tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.
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    Article
    Synthesis, Characterization, and Evaluation of the Antimicrobial Activities of Silver Nanoparticles From Cyclotrichium Origanifolium L.
    (2022) Baran, M. Fırat; Keskin, Cumali; Erbay, Nazım; Aktepe, Necmettin; Baran, Ayşe
    Cyclotricium origanifolium is a plant belonging to the Lamiaceae family and is a species that grows in the Western and Southern Anatolian regions of Turkey. In our study, the antimicrobial activities of silver nanoparticles (AgNP) were investigated through Cyclotricium origanifolium plant extract. Characterization processes of the obtained AgNPs, suitable spectral analysis methods; Uv-Vis was determined by FT-IR, SEM-EDX, XRD. According to the results of the analysis, it was determined that the AgNPs were spherical in shape and had an average diameter of 17.60 nm. The antimicrobial effect of AgNPs was determined by the minimum inhibition concentration (MIC) method. Gram positive as test microorganisms; Staphylococcus aureus, Bacillus subtilis and gram negative; Escherichia coli, Pseudomonas aeruginosa bacteria, and Candida albicans fungal pathogen species were used. The suppression of microorganism growth was investigated by comparing the efficacy of standard antibiotics used in our study with AgNPs produced by the green synthesis method. It has been observed that the obtained AgNPs have a very strong effect on gram-positive B. subtilis and gram-negative E. coli bacteria, and are more effective against C. albicans than the normal antifungal drug. It was determined that the antimicrobial activity of AgNPs produced from C. origanifolium L. plants showed a stronger effect than standard antibiotics.
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    Master Thesis
    Rumex acetosella l. (kuzukulağı) bitkisinden gümüş nanopartiküllerin sentezi, karakterizasyonu ve antimikrobiyal aktivitelerinin değerlendirilmesi
    (Mardin Artuklu Üniversitesi, 2022) Bütüner, Hafize; Aktepe, Necmettin
    Rumex acetosella L. (kuzukulağı), Polygonaceae familyasına mensup bir bitki olup ülkemiz genelinde doğal olarak yetişen bir türdür. Bu tez çalışmasında Rumx acetosella L. bitkisinden yeşil sentez yöntemi ile elde edilen gümüş nanopartiküllerin (AgNP) karakterizasyonu ve antimikrobiyal aktiviteleri incelenmiştir. Araştırmanın ilk aşamasında doğal yetiştiği ortamından toplanan bitki örneklerinden elde edilen bitki özütü ile AgNP'ler başarılı bir şekilde sentezlenmiştir. Elde edilen Ra-AgNP'lerin karakterizasyonu uygun analiz yöntemleri kullanılarak (UV-vis, FT-IR, XRD, SEM-EDX, TEM, Zeta Potansiyeli ve Zeta Sizer) tespit edilmiştir. Analiz sonuçlarına göre AgNP'lerin 476 nm dalga boyunda maksimum absorbans gösterdiği; beşgen, altıgen ve küresel görünüm; 29.16 nm boyut; -¬9.88 mV zeta potansiyeline sahip oldukları belirlenmiştir. AgNP'lerin antimikrobiyal aktiviteleri gram pozitif Staphylococcus aureus, Bacillus subtilis ve gram negatif Pseudomonas aeruginosa, Escherichia coli bakterileri ile Candida albicans mantarı üzerinde MİK (Minimum İnhibisyon Konsantrasyonu) değerlerinin belirlendiği mikrodilüsyon yöntemi ile tespit edilmiştir. Sonuç olarak Ra-AgNP'lerin hem düşük konsantrasyonlarda hem de ticari antibiyotiklere kıyasla daha güçlü antimikrobiyal aktivite gösterdiği belirlenmiştir.
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    Master Thesis
    Cyclotrichium origanifolium L. bitkisinden gümüş nanopartiküllerin sentezi, karakterizasyonu ve antimikrobiyal aktivitelerinin değerlendirilmesi
    (Mardin Artuklu Üniversitesi, 2021) Erbay, Nazım; Bütüner, Hafize; Aktepe, Necmettin
    Cyclotrichium origanifolium, Lamiaceae familyasaına ait bir bitki olup, ülkemizde Batı ve Güney Anadolu bölgelerinde yetişen bir türdür. Bu tez çalışmasında Cyclotrichium origanifolium bitkisinden biyolojik yolla elde edilen gümüş nanopartiküllerin (AgNP) antimikrobiyal aktiviteleri incelendi. Araştırmanın ilk bölümünde doğal ortamından toplanan bitki örneklerinden elde edilen özütten AgNP'ler başarılı bir şekilde üretildi. Elde edilen AgNP'lerin karakterizasyon işlemleri uygun spektral analiz yöntemleri; Uv-Vis, FT-IR, SEM-EDX, XRD ile belirlendi. Analiz sonuçlarına göre AgNP'lerin küresel şekilli ve ortalama 17,60 nm çapında olduğu tespit edildi. AgNP'lerin antimikrobiyal etkisi MİK yöntemi ile belirlendi. Test mikroorganizmaları olarak gram pozitif; S. aureus, B. subtilis ve gram negatif; E. coli, P. aeruginosa bakterileri ile C. albicans fungal patojen türleri kullanıldı. Mikroorganizma gelişimlerinin baskılanması, çalışmamızda kullanılan standart antibiyotikler ile yeşil sentez yöntemle üretilen AgNP'lerin etkinlikleri karşılaştırılarak incelendi. Sonuç olarak Cyclotrichium origanifolium bitkisinden üretilen AgNP'lerin antimikrobiyal aktivitesi, standart antibiyotiklere göre daha güçlü etki gösterdiği gözlendi.
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    Article
    Green synthesis and antimicrobial effects of silver nanoparticles by pumpkin cucurbita maxima fruit fiber
    (Medicine Science, 2022) Aktepe, Necmettin; Baran, Ayşe
    In the present study, for the first time, green synthesis of silver nanoparticles was carried out with the extract obtained from the fibrous structure in the pumpkin Cucurbita maxima (CM) fruit. The presence of silver nanoparticles formed by the reduction of Ag+ ions was determined with the maximum absorbance values of 442.89 nm in wavelength scans made by UV-Vis spectroscopy analysis. The functional groups of the phytochemicals involved in the reduction were examined by Fourier-transform infrared (FT-IR) spectroscopy. For the morphology of the synthesized AgNPs, they were found to be uniform in spherical appearance using atomic power microscopy (AFM), transmission electron microscopy (TEM), and field emission scanning electron microscopy (FE-SEM) images. The surface charges of AgNPs were determined to be -16.4 mV by Zeta potential analysis. Their crystal structures and nano-sizes were evaluated by X-ray diffraction (XRD) data to be in cubic pattern, and their size was 21.20 nm using the Debye-Scherrer equation. AgNPs (CM-AgNPs) synthesized via CM provided growth suppression at concentrations of 1.00-0.12, 2.00-1.00, and 0.50 µg/mL on pathogen gram-positive, gram-negative strains and the fungus Candida albicans, respectively. These concentrations were effective against antibiotics and silver solution at a very low concentration.
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    Article
    Citation - WoS: 1
    Removal of Heavy Metals in Water by Biosorption Method Using Three Different Bacillus sp-derived Biosorbents
    (Kahramanmaraş Sütçü İmam Üniversitesi, 2022) Baran, Mehmet Fırat; Düz, Zahir; Baran, Ayşe; Keskin, Cumali; Aktepe, Necmettin; Düz, M.zahir
    Water is an important part of the ecosystem for life. With industrialization, pollution in water resources has reached a worrying level. Water pollution due to heavy metals and their increasing concentrations have caused researchers to increase their interest in the subject due to the damage they cause to water ecosystems. It requires serious cost and time to eliminate the pollution caused by heavy metals in water. In recent, years, the use of biosorption method using bacteria to remove heavy metals in water has become widespread. The main reason why this method is preferred is that gram-positive bacteria have a thick peptidoglycan layer on the cell wall and increases the adsorption capacity. In this study. in drinking, waste, river water and artificially prepared samples, batch method of heavy metal biosorption and biosorption competition in multiple prepared heavy metal solutions were investigated. For these processes. Bacillus licheniformth sp. Bacillus subtilis sp. and Bacillus subtilis (ATCC 6051) strains were used as a biosorbent. Biosorbtion of Cd (II), Cu (H), Pb (II), Fe(II), Ni (II) and Zn (II) metals from waters with these biosorbents at different pHs at. 25 degrees C with 0.25 mg LI It was investigated. Surface morphological structures of biosorbents were evaluated using SEM images and element compositions were evaluated using EDAX profile. Element content was determined using ICP-OES. It was found that heavy metal ions were removed up to 98% with maximum biosorption at pH 6.0.