Aktepe, Necmettin

Aktepe, Necmettin

Name Variants

Aktepe, N.

Job Title

Doçent

Email Address

necmettinaktepe@gmail.com

Main Affiliation

Department of Nursing / Hemşirelik Bölümü

Scholarly Output

21

Articles

18

Citation Count

77

Supervised Theses

2 Scholarly Output Search Results

Now showing 1 - 10 of 18

The protective effects of different parts of hypericum perforatum extracts on human mononuclear leukocytes in hydrogen peroxide-induced DNA damage and their phenolic contents
(Medicine Science, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Baran, Mehmet Fırat
Oxidative stress is the state of the formation of some pathophysiological condition with the excessive increase of the normal amount of free radicals in the organism. In this study, the in vivo genotoxic and antigenotoxic effects of methanol and water extract and phenolic content of Hypericum perforatum flower, fruit, and seed methanol extracts were analyzed. HPLC was used to evaluate the quantities of 3,4-Dihydroxybenzoic acid, syringic acid, hydroxycinnamic acid, O-coumaric acid, caffeic acid, and catechin in the methanol extracts. The alkaline comet test was used to assess the DNA damage and protective effects of H. perforatum flower fruit, seed methanol, and water extract on human mononuclear leukocytes. The amounts of catechin and caffeic acid in seed methanol extract were found as quite high when compared to other extracts. The highest protective effects were seen at 10 and 50μg/ml concentrations of seed methanol extract. The optimum doses of fruit, flower, and seed extracts obtained from H. perforatum neutralized the genotoxic effect. This effect is stronger in seed methanol extract than other extracts. We suggest that more research is needed to evaluate the effects of H. perforatum phytochemicals in vitro and in vivo.
Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications
(Taylor & Francis Online, 2021) Keskin, Cumali; Aktepe, Necmettin; Baran, Ayşe; Baran, Mehmet Fırat; Aktepe, Necmettin; Yavuz, Ömer; İrtegun Kandemir, Sevgi
In this study, silver nanoparticles (AgNPs) were synthesized economically and simply using an environmentally friendly method with the extract obtained from agricultural waste olive leaves. AgNPs synthesized according to the analysis data were determined to have maximum absorbance at 433.5 nm wavelength, spherical appearance, 7.2 nm crystal nano size and -19.9 mV zeta potential. It was determined by the microdilution method with Minimum Inhibition Concentration (MIC) that AgNPs exert a suppressive effect on the growth of pathogen gram-negative, positive bacteria and yeast at very low concentrations. The cytotoxic effects of the particles were investigated on healthy cell lines (HDF) and cancerous cell lines (U118, CaCo-2, Skov-3). AgNPs showed up to 70% suppression in cancer cell lines.
Determination of chemical composition and antioxidant, cytotoxic, antimicrobial, and enzyme inhibition activities of Rumex acetosella L. plant extract
(Springer int Publ Ag, 2024) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Atalar, Mehmet Nuri; Keskin, Cumali; Khalilov, Rovshan
Purpose The phenolic composition, antioxidant, antimicrobial activity, enzyme inhibition activity, and cytotoxic activity potentials of the plant Rumex acetosella L. (R. acetosella) were examined in this study. Materials and Methods: The chemical composition of R. acetosella methanol extract was identified by the LC-MS/MS method. The antioxidant activity was tested using beta-carotene/linoleic acid, DPPH free radical scavenging, ABTS cation radical scavenging, CUPRAC reducing power, and metal chelating activity methods. The cytotoxic activity was determined by the MTT assay using human ovarian adenocarcinoma (Skov-3), glioblastoma (U87), human dermal fibroblasts (HDF), and human colorectal adenocarcinoma (CaCo-2) cell lines. The antimicrobial activity of methanolic extracts was tested on gram-negative (Escherichia coli and Pseudomonas aeuriginosa) and gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) using the in vitro minimum inhibition concentration method (MIC). Enzyme inhibition activity of R. acetosella methanol extract was measured spectrophotometrically against acetylcholinesterase (AChE) and glutathione S-transferase (GST) enzymes. Results: The findings showed that the major components of the methanol extract content were luteolin-7-O-glucoside (1.599 m/L), polydatin (91,024 m/L), and shikimic acid (0.773 m/L). It was determined that the extract and standard antioxidant (a-tocopherol) results in DPPH center dot, and ABTS center dot + tests performed to determine the antioxidant activity were close to each other, and this value was more effective than the standard antioxidant (alpha-tocopherol) in the CUPRAC test. These results suggested that the plant's antioxidant potential was higher when compared with reference antioxidant compounds. It was determined that the methanol extract of R. acetosella had a weaker effect on the growth of the tested microorganisms than the antibiotics used as standard. The activity of the GST and AChE enzymes was found to be severely inhibited by the methanol extract of R. acetosella. Conclusion: Based on these findings, R. acetosella L. is a medicinal and commercially beneficial plant that warrants further investigation.
Induction of apoptosis in human hormone-refractory prostate cancer cell lines by using resveratrol in combination with AT-101
(Wiley, 2021) Aktepe, Necmettin; Yukselten, Yunus
The aim of this study was to determine the appropriate doses of AT-101 and resveratrol combination in the in vitro hormone-refractory prostate cancer (PC) cell lines, in order to evaluate the cytotoxic and genotoxic effects of this combination on the proliferation of cancer cells, namely PC-3, DU-145 and LNCAP. Cytotoxicity in PC cell lines was analysed by using the XTT Cell Proliferation Assay. DNA damage was performed with the cell death assay. Apoptotic protein levels were performed by Roche Human Apoptosis Array. IC50 values were determined by XTT analysis. The strongest combined doses (100 µM resveratrol + 5µM AT-101) were found to have the strongest synergistic apoptotic and cytotoxic effects on DU-145 cells at 72 hr. While the combined use of resveratrol and AT-101 increased the expression of markers in apoptotic cell pathways on cells, a decrease in the expression of anti-apoptotic markers was detected (p ˂ 0.05). Combined applications of these compounds showed an important synergism in the hormone-refractory PC cell lines, and it was determined that after the post-translational modification, they were significantly effective on the apoptotic pathway. These results have revealed that the combination of resveratrol and AT-101 holds great expectation as a new chemotherapeutic application in the treatment of human prostate cancer.
Fast and Low-Cost Biosynthesis of AgNPs with Almond Leaves: Medical Applications with Biocompatible Structures
(Progress in Nutrition, 2021) Aktepe, Necmettin; Baran, Ayşe
In this study, silver nanoparticles (AgNPs) were obtained in a low cost, easy and simple way by using the leaf extract of Prunus dulcis L. (almond tree) growing in the Mardin region. Characterization of AgNPs obtained by biosynthesis: Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffractometer (XRD), UV-visible spectrophotometer (UV-Vis.), Scanning Electron Microscope (SEM), Transmission Electron Microscope(TEM), Zeta potential and Zeta size analysis was done through data. It was determined that AgNPs have maximum absorbance at 443 nm wavelength, they exhibit 14.67 crystal nano size, -19.9 mV zeta potential in spherical appearance. The Minimum Inhibition Concentration (MIC) of the obtained AgNPs was determined by using microdilution method on the growth of pathogen strains.
Gümüş Nano Materyallerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktiviteleri
(2021) Aktepe, Necmettin
Gümüş nano materyallerin kullanım alanları oldukça yaygındır. Farklı sentezleme yöntemleri ile elde edilebilirler. Bu çalışmada, gümüş nano materyaller (AgNP’ler) biyosentez yöntemi kullanılarak ekonomik ve basit bir şekilde elde edildi. Sentez sonucunda elde edilen AgNP’ler UV-visiblespektrofotometre (UV-Vis.), Taramalı Elektron Mikroskobu (SEM), X- Işınımı Kırınımı Difraktrometresi (XRD), Fourier dönüşümü kızılötesi spektroskopisi (FTIR), Zeta potansiyeli cihazları kullanılarak karakterize edildi. AgNP’lerin 426.66 nm dalga boyunda maksimum absorbansa sahip oldukları, küresel morfolojik yapı sergiledikleri, 17.68 nm kristal nano boyut ve yüzey yüklerinin -20.9 mV zeta potansiyeli dağılımı gösterdiği değerlendirildi. Patojen mikroorganizmalar üzerinde Minumum İnhibisyon Konsantrasyonları (MİK) 0.03-0.5 mg/L olarak mikrodilusyon yöntemi kullanılarak tespit edildi.
Farklı Biyosorbentler Kullanarak Su Ortamında Ağır Metallerin Biyosorbsiyon Metodu ile Giderilmesi
(2022) Keskin, Cumali; Aktepe, Necmettin; Baran, Mehmet Fırat; Baran, Ayşe; Baran, Ayşe
Su canlılar için ekosistemin önemli parçasıdır. Endüstrileşme ile birlikte su kaynaklarındaki kirlenme endişe edilir boyutlara ulaşmıştır. Ağır metallere bağlı su kirliliği ve artan konsantrasyonları su ekosistemlerine verdiği zarar nedeniyle araştırmacıların konuya olan ilgisinin artmasına sebep olmuştur. Ağır metallerin sularda oluşturduğu kirliliği gidermek ciddi maliyet ve zaman gerektirmektedir. Son yıllarda suda bulunan ağır metallerin uzaklaştırılması için bakteriler kullanılarak biyosorbsiyon yönteminin kullanılması yaygınlaşmıştır. Bu yöntemin tercih edilmesinin temel sebebi gram pozitif bakterilerin hücre duvarında kalın bir peptidoglikan tabakasına sahip olması ile adsorbsiyon kapasitesini artırmasıdır. Bu çalışmada içme, atık, nehir suları ve suni olarak hazırlanan numunelerde çalkalamalı metod kullanarak ağır metal biyosorbsiyonu ile birlikte çoklu hazırlanmış ağır metal çözeltilerinde biyosorbsiyon rekabeti incelenmiştir. Bu işlemler için Dicle nehri bölgesine ait topraklardan izole edilen Bacillus licheniformis sp. Bacillus subtilis sp. ve Bacillus subtilis (ATCC 6051) suşları ile sulu çözeltiden Cd (II), Cu (II), Pb (II), Fe (II), Ni (II) ve Zn (II) metallerinin biyosorbsiyonu değerlendirildi. B. subtilis suşları ve B.licheniformis sp. organizmalarının yüzey morfoljik yapıları SEM görüntüleri, element kompozisyonları EDAX verileri ile incelendi. ICP-OES kullanılarak element içeriği tespit edildi. Sulu çözeltideki Cd (II), Cu (II), Pb (II), Fe (II), Ni (II) ve Zn (II) metal iyonları farklı pH’larda 25 °C de 0,25 mg L-1 biomass ile biyosorbsiyon gerçekleştirildi. pH 6.0 da maksimum biyosorbsiyon ile metal iyonlarının % 98 varan oranda giderildiği belirlendi.
Morin Attenuates Arsenic-Induced Toxicity in 3t3 Embryonic Fibroblast Cells by Suppressing Oxidative Stress, Inflammation, and Apoptosis: in Vitro and Silico Evaluations
(Oxford Univ Press, 2024) Unsal, Velid; Aktepe, Necmettin; Aktepe, Necmettin; Oner, Erkan
This study aims to investigate the curative effects of Morin, a flavonoid, against arsenic toxicity in 3T3 embryonic fibroblast cells and its effect on the molecular mechanisms of cells. The cytotoxicity and viability of the cells were measured by MTT and LDH tests. Arsenic (0.74 mu M) was used to trigger toxicity and Morin (50 mu M) was used for treatment. The levels of oxidative stress biomarkers and the activities of antioxidant enzymes were measured by spectrophotometric method, and inflammatory markers were measured by ELISA method. While mRNA expression levels of Bax, Bcl-2 levels, and Caspase-3 activity were measured by qRT-PCR technique, TUNEL staining was performed to detect DNA breaks and DAPI staining to visualize nuclear changes. Protein structures were retrieved from the protein data bank. OpenBabel and Autodock programs were used for the molecular docking study. Morin rescued the 3T3 embryonic fibroblast cells exposed to arsenic. However, Arsenic decreased the activities of antioxidant enzymes in cells and significantly increased oxidative stress, inflammation, and apoptosis. Morin treatment reduced oxidative damage and TNF-alpha and IL-1 beta levels. Arsenic-induced Caspase-3 mRNA expression level and Bax protein mRNA expression level were significantly increased, while Bcl-2 mRNA expression level was significantly decreased. While Caspase-3 mRNA expression level and Bax protein mRNA expression level decreased with morin treatment, Bcl-2 mRNA expression level increased significantly. Molecular docking study results showed good binding affinity of morin in SOD, GSH-Px, Bax, Bcl-2, Caspase-3, TNF-alpha, and IL-1 beta structures. Morin showed antioxidant, anti-inflammatory, and anti-apoptotic effects against Arsenic-induced cellular toxicity. Graphical Abstract
Biosynthesis of AgNPs by extract from waste leaves of Citrullus lanatus sp. (watermelon); characterization, antibacterial and antifungal effects
(Progress in Nutrition, 2021) Aktepe, Necmettin; Baran, Ayşe
Silver nanoparticles (AgNPs) are valuable materials with a large number of sectors used. Green synthesis is very important for biomedical applications as they show biocompatible properties. In this study, AgNPs were easily synthesized using the environmentally friendly green synthesis approach using agricultural waste parts of Citrullus lanatus sp. plant grown in Diyarbakır region. Characterization of synthesized AgNPs was made. Fourier Transform Infrared Spectroscopy (FTIR) analysis was used to evaluate the phytochemicals responsible for effective reduction in the formation of AgNPs. UV-visible spectrophotometer (UV-Vis.) Spectra were also used to determine the presence of AgNPs. X-Ray Diffraction Diffractometer (XRD), Scanning Electron Microscope (SEM), Transmission Electron Microscope, Zeta potential analyzes were performed to define the crystal structures, dimensions and surface charges of AgNPs, respectively. In these data, it was determined that AgNPs showed maximum absorbance at 460 nm, spherical appearance, 21.27 crystal nano size and -30.05 mV zeta potential. Antimicrobial effects of AgNPs on gram positive Staphylococcus aureus (S. aureus) and Bacillus subtilis (B. subtilis) bacteria, gram negative Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) bacteria, as well as on fungus C. albicans pathogenic microorganisms It was analyzed by specifying the Minimum Inhibition Concentration (MIC) by microdilution.
Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
(Wiley Online Library, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Taşkin, Abdullah; Yavuz, Ömer; Demirtaş, İbrahim; Oğuz, Ercan; Jahan, Israt
Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography–electrospray ionization– tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.