Aktepe, Necmettin

Aktepe, Necmettin

Name Variants

Aktepe, N.

Job Title

Doçent

Email Address

necmettinaktepe@gmail.com

Main Affiliation

Department of Nursing / Hemşirelik Bölümü

Sustainable Development Goals Report Points

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Scholarly Output

21

Articles

18

Citation Count

77

Supervised Theses

2 Scholarly Output Search Results

Now showing 1 - 10 of 21

Synthesis, Characterization and Evaluation of Antimicrobial Activities of Silver Nanoparticles Obtained From Rumex Acetosella L. (sorrel) Plant
(2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Baran, Mehmet Fırat; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
Rumex acetosella L. (sorrel) is a plant belonging to the Polygonaceous family and is a species that grows naturally across Turkey. In this study, the characterization of silver nanoparticles (AgNPs) obtained from the Rumex acetocella L. (RA) plant using the green synthesis method was performed and their antimicrobial activities were investigated. AgNPs were successfully synthesized in the first stage of the study using plant extract taken from plant samples collected from the natural growing environment. Characterization of synthesized AgNPs was performed using appropriate analytical methods (UV-vis, FT-IR, XRD, SEM-EDX, TEM, Zeta Potential and Zeta Sizer). According to the analysis results, it was determined that AgNPs had a maximum absorbance at 476 nm wavelength, a pentagonal, hexagonal, and spherical appearance, a size of 29.16 nm, and a zeta potential of -9.88 mV. The antimicrobial activities of AgNPs were tested using the microdilution technique, in which Minimum Inhibition Concentration (MIC) values were determined on gram-positive Staphylococcus aureus, Bacillus subtilis and gram-negative Pseudomonas aeruginosa, Escherichia coli bacteria and Candida albicans fungus. It showed a very strong antimicrobial effect on C. albicans, S. aureus and P. aeruginosa. Consequently, AgNPs had stronger antimicrobial activity at low concentrations and when compared to commercial antibiotics.
Synthesis, Characterization, and Evaluation of the Antimicrobial Activities of Silver Nanoparticles From Cyclotrichium Origanifolium L.
(2022) Keskin, Cumali; Aktepe, Necmettin; Baran, Ayşe; Aktepe, Necmettin; Baran, Ayşe; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
Cyclotricium origanifolium is a plant belonging to the Lamiaceae family and is a species that grows in the Western and Southern Anatolian regions of Turkey. In our study, the antimicrobial activities of silver nanoparticles (AgNP) were investigated through Cyclotricium origanifolium plant extract. Characterization processes of the obtained AgNPs, suitable spectral analysis methods; Uv-Vis was determined by FT-IR, SEM-EDX, XRD. According to the results of the analysis, it was determined that the AgNPs were spherical in shape and had an average diameter of 17.60 nm. The antimicrobial effect of AgNPs was determined by the minimum inhibition concentration (MIC) method. Gram positive as test microorganisms; Staphylococcus aureus, Bacillus subtilis and gram negative; Escherichia coli, Pseudomonas aeruginosa bacteria, and Candida albicans fungal pathogen species were used. The suppression of microorganism growth was investigated by comparing the efficacy of standard antibiotics used in our study with AgNPs produced by the green synthesis method. It has been observed that the obtained AgNPs have a very strong effect on gram-positive B. subtilis and gram-negative E. coli bacteria, and are more effective against C. albicans than the normal antifungal drug. It was determined that the antimicrobial activity of AgNPs produced from C. origanifolium L. plants showed a stronger effect than standard antibiotics.
Citation - WoS: 0
Removal of Heavy Metals in Water by Biosorption Method Using Three Different Bacillus sp-derived Biosorbents
(Kahramanmaraş Sütçü İmam Üniversitesi, 2022) Keskin, Cumali; Aktepe, Necmettin; Baran, Mehmet Fırat; Baran, Ayşe; Aktepe, Necmettin; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
Water is an important part of the ecosystem for life. With industrialization, pollution in water resources has reached a worrying level. Water pollution due to heavy metals and their increasing concentrations have caused researchers to increase their interest in the subject due to the damage they cause to water ecosystems. It requires serious cost and time to eliminate the pollution caused by heavy metals in water. In recent, years, the use of biosorption method using bacteria to remove heavy metals in water has become widespread. The main reason why this method is preferred is that gram-positive bacteria have a thick peptidoglycan layer on the cell wall and increases the adsorption capacity. In this study. in drinking, waste, river water and artificially prepared samples, batch method of heavy metal biosorption and biosorption competition in multiple prepared heavy metal solutions were investigated. For these processes. Bacillus licheniformth sp. Bacillus subtilis sp. and Bacillus subtilis (ATCC 6051) strains were used as a biosorbent. Biosorbtion of Cd (II), Cu (H), Pb (II), Fe(II), Ni (II) and Zn (II) metals from waters with these biosorbents at different pHs at. 25 degrees C with 0.25 mg LI It was investigated. Surface morphological structures of biosorbents were evaluated using SEM images and element compositions were evaluated using EDAX profile. Element content was determined using ICP-OES. It was found that heavy metal ions were removed up to 98% with maximum biosorption at pH 6.0.
The protective effects of different parts of hypericum perforatum extracts on human mononuclear leukocytes in hydrogen peroxide-induced DNA damage and their phenolic contents
(Medicine Science, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Baran, Mehmet Fırat; Department of Nursing / Hemşirelik Bölümü; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Oxidative stress is the state of the formation of some pathophysiological condition with the excessive increase of the normal amount of free radicals in the organism. In this study, the in vivo genotoxic and antigenotoxic effects of methanol and water extract and phenolic content of Hypericum perforatum flower, fruit, and seed methanol extracts were analyzed. HPLC was used to evaluate the quantities of 3,4-Dihydroxybenzoic acid, syringic acid, hydroxycinnamic acid, O-coumaric acid, caffeic acid, and catechin in the methanol extracts. The alkaline comet test was used to assess the DNA damage and protective effects of H. perforatum flower fruit, seed methanol, and water extract on human mononuclear leukocytes. The amounts of catechin and caffeic acid in seed methanol extract were found as quite high when compared to other extracts. The highest protective effects were seen at 10 and 50μg/ml concentrations of seed methanol extract. The optimum doses of fruit, flower, and seed extracts obtained from H. perforatum neutralized the genotoxic effect. This effect is stronger in seed methanol extract than other extracts. We suggest that more research is needed to evaluate the effects of H. perforatum phytochemicals in vitro and in vivo.
Citation - WoS: 17
Citation - Scopus: 14
In-Vitro Antioxidant, Cytotoxic, Cholinesterase Inhibitory Activities and Anti-Genotoxic Effects of Hypericum Retusum Aucher Flowers, Fruits and Seeds Methanol Extracts in Human Mononuclear Leukocytes
(Iranian Journal of Pharmaceutical Research, 2017) Keskin, Cumali; Aktepe, Necmettin; Yükselten, Y.; Asuman,; Boğa, M.; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
The present study investigates the antioxidant, anticancer, anticholinesterase, anti-genotoxic activities and phenolic contents of flower, fruit and seed methanol extracts of Hypericum retusum AUCHER. The amounts of protocatechuic acid, catechin, caffeic acid and syringic acid in methanol extracts were determined by HPLC. Total phenolic content of H. retusum seed extract was found more than fruit and flower extracts. The DPPH free radical scavenging activity of flower and seed methanol extracts showed close activity versus BHT as control. Among three extracts of H. retusum only flower methanol extract was exhibited considerable cytotoxic activities against to HeLa and NRK-52E cell lines. Moreover, seed methanol extract showed both acetyl and butyrl-cholinesterase inhibitory activity. The highest anti-genotoxic effects were seen 25 and 50 μg/mL concentrations. In this study, the extracts showed a strong antioxidant and anti-genotoxic effect. The seed extract was more efficient- than extracts of fruit and flowers. Our results suggest that the antioxidant and anti-genotoxic effects of extracts depend on their phenolic contents. Further studies should evaluate the in-vitro and in-vivo the benefits of H. retusum seed methanol extracts. © 2017 by School of Pharmacy.
Cyclotrichium origanifolium L. bitkisinden gümüş nanopartiküllerin sentezi, karakterizasyonu ve antimikrobiyal aktivitelerinin değerlendirilmesi
(Mardin Artuklu Üniversitesi, 2021) Aktepe, Necmettin; Aktepe, Necmettin; Department of Nursing / Hemşirelik Bölümü
Cyclotrichium origanifolium, Lamiaceae familyasaına ait bir bitki olup, ülkemizde Batı ve Güney Anadolu bölgelerinde yetişen bir türdür. Bu tez çalışmasında Cyclotrichium origanifolium bitkisinden biyolojik yolla elde edilen gümüş nanopartiküllerin (AgNP) antimikrobiyal aktiviteleri incelendi. Araştırmanın ilk bölümünde doğal ortamından toplanan bitki örneklerinden elde edilen özütten AgNP'ler başarılı bir şekilde üretildi. Elde edilen AgNP'lerin karakterizasyon işlemleri uygun spektral analiz yöntemleri; Uv-Vis, FT-IR, SEM-EDX, XRD ile belirlendi. Analiz sonuçlarına göre AgNP'lerin küresel şekilli ve ortalama 17,60 nm çapında olduğu tespit edildi. AgNP'lerin antimikrobiyal etkisi MİK yöntemi ile belirlendi. Test mikroorganizmaları olarak gram pozitif; S. aureus, B. subtilis ve gram negatif; E. coli, P. aeruginosa bakterileri ile C. albicans fungal patojen türleri kullanıldı. Mikroorganizma gelişimlerinin baskılanması, çalışmamızda kullanılan standart antibiyotikler ile yeşil sentez yöntemle üretilen AgNP'lerin etkinlikleri karşılaştırılarak incelendi. Sonuç olarak Cyclotrichium origanifolium bitkisinden üretilen AgNP'lerin antimikrobiyal aktivitesi, standart antibiyotiklere göre daha güçlü etki gösterdiği gözlendi.
Citation - WoS: 6
Citation - Scopus: 5
Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
(Wiley Online Library, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Taşkin, Abdullah; Yavuz, Ömer; Demirtaş, İbrahim; Oğuz, Ercan; Jahan, Israt; Department of Nursing / Hemşirelik Bölümü; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography–electrospray ionization– tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.
Green synthesis and antimicrobial effects of silver nanoparticles by pumpkin cucurbita maxima fruit fiber
(Medicine Science, 2022) Aktepe, Necmettin; Baran, Ayşe; Department of Nursing / Hemşirelik Bölümü
In the present study, for the first time, green synthesis of silver nanoparticles was carried out with the extract obtained from the fibrous structure in the pumpkin Cucurbita maxima (CM) fruit. The presence of silver nanoparticles formed by the reduction of Ag+ ions was determined with the maximum absorbance values of 442.89 nm in wavelength scans made by UV-Vis spectroscopy analysis. The functional groups of the phytochemicals involved in the reduction were examined by Fourier-transform infrared (FT-IR) spectroscopy. For the morphology of the synthesized AgNPs, they were found to be uniform in spherical appearance using atomic power microscopy (AFM), transmission electron microscopy (TEM), and field emission scanning electron microscopy (FE-SEM) images. The surface charges of AgNPs were determined to be -16.4 mV by Zeta potential analysis. Their crystal structures and nano-sizes were evaluated by X-ray diffraction (XRD) data to be in cubic pattern, and their size was 21.20 nm using the Debye-Scherrer equation. AgNPs (CM-AgNPs) synthesized via CM provided growth suppression at concentrations of 1.00-0.12, 2.00-1.00, and 0.50 µg/mL on pathogen gram-positive, gram-negative strains and the fungus Candida albicans, respectively. These concentrations were effective against antibiotics and silver solution at a very low concentration.
Citation - WoS: 19
Citation - Scopus: 21
Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications
(Taylor & Francis Online, 2021) Keskin, Cumali; Aktepe, Necmettin; Baran, Ayşe; Baran, Mehmet Fırat; Aktepe, Necmettin; Yavuz, Ömer; İrtegun Kandemir, Sevgi; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
In this study, silver nanoparticles (AgNPs) were synthesized economically and simply using an environmentally friendly method with the extract obtained from agricultural waste olive leaves. AgNPs synthesized according to the analysis data were determined to have maximum absorbance at 433.5 nm wavelength, spherical appearance, 7.2 nm crystal nano size and -19.9 mV zeta potential. It was determined by the microdilution method with Minimum Inhibition Concentration (MIC) that AgNPs exert a suppressive effect on the growth of pathogen gram-negative, positive bacteria and yeast at very low concentrations. The cytotoxic effects of the particles were investigated on healthy cell lines (HDF) and cancerous cell lines (U118, CaCo-2, Skov-3). AgNPs showed up to 70% suppression in cancer cell lines.
Citation - WoS: 0
Citation - Scopus: 0
Determination of chemical composition and antioxidant, cytotoxic, antimicrobial, and enzyme inhibition activities of Rumex acetosella L. plant extract
(Springer int Publ Ag, 2024) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Atalar, Mehmet Nuri; Keskin, Cumali; Khalilov, Rovshan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
Purpose The phenolic composition, antioxidant, antimicrobial activity, enzyme inhibition activity, and cytotoxic activity potentials of the plant Rumex acetosella L. (R. acetosella) were examined in this study. Materials and Methods: The chemical composition of R. acetosella methanol extract was identified by the LC-MS/MS method. The antioxidant activity was tested using beta-carotene/linoleic acid, DPPH free radical scavenging, ABTS cation radical scavenging, CUPRAC reducing power, and metal chelating activity methods. The cytotoxic activity was determined by the MTT assay using human ovarian adenocarcinoma (Skov-3), glioblastoma (U87), human dermal fibroblasts (HDF), and human colorectal adenocarcinoma (CaCo-2) cell lines. The antimicrobial activity of methanolic extracts was tested on gram-negative (Escherichia coli and Pseudomonas aeuriginosa) and gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) using the in vitro minimum inhibition concentration method (MIC). Enzyme inhibition activity of R. acetosella methanol extract was measured spectrophotometrically against acetylcholinesterase (AChE) and glutathione S-transferase (GST) enzymes. Results: The findings showed that the major components of the methanol extract content were luteolin-7-O-glucoside (1.599 m/L), polydatin (91,024 m/L), and shikimic acid (0.773 m/L). It was determined that the extract and standard antioxidant (a-tocopherol) results in DPPH center dot, and ABTS center dot + tests performed to determine the antioxidant activity were close to each other, and this value was more effective than the standard antioxidant (alpha-tocopherol) in the CUPRAC test. These results suggested that the plant's antioxidant potential was higher when compared with reference antioxidant compounds. It was determined that the methanol extract of R. acetosella had a weaker effect on the growth of the tested microorganisms than the antibiotics used as standard. The activity of the GST and AChE enzymes was found to be severely inhibited by the methanol extract of R. acetosella. Conclusion: Based on these findings, R. acetosella L. is a medicinal and commercially beneficial plant that warrants further investigation.