Aktepe, Necmettin
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Aktepe, N.
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Doçent
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necmettinaktepe@gmail.com
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Department of Nursing / Hemşirelik Bölümü
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17
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0
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2
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0
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5
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0
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6
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2
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13
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10
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16
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8
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15
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3
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8
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9
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1
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14
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2
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4
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1
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7
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11
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2
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12
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0
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11
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115
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6
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21
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243
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23
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19
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136/1900
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2
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0
WoS Citation Count
114
Scopus Citation Count
115
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6
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6
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6
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4.96
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5.00
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14
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| Journal | Count |
|---|---|
| International Journal of Agriculture, Environment and Food Sciences | 3 |
| Medicine Science | 2 |
| Progress in Nutrition | 2 |
| Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi | 2 |
| Chemical Papers | 1 |
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Now showing 1 - 10 of 23
Article Synthesis, Characterization and Evaluation of Antimicrobial Activities of Silver Nanoparticles Obtained From Rumex Acetosella L. (sorrel) Plant(2022) Aktepe, Necmettin; Bütüner, Hafize; Baran, Ayşe; Keskin, Cumali; Baran, Mehmet FıratRumex acetosella L. (sorrel) is a plant belonging to the Polygonaceous family and is a species that grows naturally across Turkey. In this study, the characterization of silver nanoparticles (AgNPs) obtained from the Rumex acetocella L. (RA) plant using the green synthesis method was performed and their antimicrobial activities were investigated. AgNPs were successfully synthesized in the first stage of the study using plant extract taken from plant samples collected from the natural growing environment. Characterization of synthesized AgNPs was performed using appropriate analytical methods (UV-vis, FT-IR, XRD, SEM-EDX, TEM, Zeta Potential and Zeta Sizer). According to the analysis results, it was determined that AgNPs had a maximum absorbance at 476 nm wavelength, a pentagonal, hexagonal, and spherical appearance, a size of 29.16 nm, and a zeta potential of -9.88 mV. The antimicrobial activities of AgNPs were tested using the microdilution technique, in which Minimum Inhibition Concentration (MIC) values were determined on gram-positive Staphylococcus aureus, Bacillus subtilis and gram-negative Pseudomonas aeruginosa, Escherichia coli bacteria and Candida albicans fungus. It showed a very strong antimicrobial effect on C. albicans, S. aureus and P. aeruginosa. Consequently, AgNPs had stronger antimicrobial activity at low concentrations and when compared to commercial antibiotics.Article Citation - WoS: 4Citation - Scopus: 3Induction of apoptosis in human hormone-refractory prostate cancer cell lines by using resveratrol in combination with AT-101(Wiley, 2021) Aktepe, Necmettin; Yukselten, YunusThe aim of this study was to determine the appropriate doses of AT-101 and resveratrol combination in the in vitro hormone-refractory prostate cancer (PC) cell lines, in order to evaluate the cytotoxic and genotoxic effects of this combination on the proliferation of cancer cells, namely PC-3, DU-145 and LNCAP. Cytotoxicity in PC cell lines was analysed by using the XTT Cell Proliferation Assay. DNA damage was performed with the cell death assay. Apoptotic protein levels were performed by Roche Human Apoptosis Array. IC50 values were determined by XTT analysis. The strongest combined doses (100 µM resveratrol + 5µM AT-101) were found to have the strongest synergistic apoptotic and cytotoxic effects on DU-145 cells at 72 hr. While the combined use of resveratrol and AT-101 increased the expression of markers in apoptotic cell pathways on cells, a decrease in the expression of anti-apoptotic markers was detected (p ˂ 0.05). Combined applications of these compounds showed an important synergism in the hormone-refractory PC cell lines, and it was determined that after the post-translational modification, they were significantly effective on the apoptotic pathway. These results have revealed that the combination of resveratrol and AT-101 holds great expectation as a new chemotherapeutic application in the treatment of human prostate cancer.Article Farklı Biyosorbentler Kullanarak Su Ortamında Ağır Metallerin Biyosorbsiyon Metodu ile Giderilmesi(2022) Düz, M.zahir; Keskin, Cumali; Aktepe, Necmettin; Baran, Mehmet Fırat; Baran, AyşeSu canlılar için ekosistemin önemli parçasıdır. Endüstrileşme ile birlikte su kaynaklarındaki kirlenme endişe edilir boyutlara ulaşmıştır. Ağır metallere bağlı su kirliliği ve artan konsantrasyonları su ekosistemlerine verdiği zarar nedeniyle araştırmacıların konuya olan ilgisinin artmasına sebep olmuştur. Ağır metallerin sularda oluşturduğu kirliliği gidermek ciddi maliyet ve zaman gerektirmektedir. Son yıllarda suda bulunan ağır metallerin uzaklaştırılması için bakteriler kullanılarak biyosorbsiyon yönteminin kullanılması yaygınlaşmıştır. Bu yöntemin tercih edilmesinin temel sebebi gram pozitif bakterilerin hücre duvarında kalın bir peptidoglikan tabakasına sahip olması ile adsorbsiyon kapasitesini artırmasıdır. Bu çalışmada içme, atık, nehir suları ve suni olarak hazırlanan numunelerde çalkalamalı metod kullanarak ağır metal biyosorbsiyonu ile birlikte çoklu hazırlanmış ağır metal çözeltilerinde biyosorbsiyon rekabeti incelenmiştir. Bu işlemler için Dicle nehri bölgesine ait topraklardan izole edilen Bacillus licheniformis sp. Bacillus subtilis sp. ve Bacillus subtilis (ATCC 6051) suşları ile sulu çözeltiden Cd (II), Cu (II), Pb (II), Fe (II), Ni (II) ve Zn (II) metallerinin biyosorbsiyonu değerlendirildi. B. subtilis suşları ve B.licheniformis sp. organizmalarının yüzey morfoljik yapıları SEM görüntüleri, element kompozisyonları EDAX verileri ile incelendi. ICP-OES kullanılarak element içeriği tespit edildi. Sulu çözeltideki Cd (II), Cu (II), Pb (II), Fe (II), Ni (II) ve Zn (II) metal iyonları farklı pH’larda 25 °C de 0,25 mg L-1 biomass ile biyosorbsiyon gerçekleştirildi. pH 6.0 da maksimum biyosorbsiyon ile metal iyonlarının % 98 varan oranda giderildiği belirlendi.Article Citation - WoS: 7Citation - Scopus: 7Biochemical components, enzyme inhibitory, antioxidant and antimicrobial activities in endemic plant Scilla mesopotamica speta(Wiley, 2021) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Atalar, Mehmet Nuri; Baran, Mehmet Fırat; Akmeşe, ŞükrüIn this study, in vitro antioxidant, antimicrobial, anticholinesterase and phenolic profile of different solvent extracts of Scilla mesopotamica speta were determined in detail. In vitro antioxidant activities and total phenolic and flavonoid contents of plant extracts obtained with different solvents were tested in terms of 2.2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities. The highest total phenolic and total flavonoid contents were determined in the ethyl acetate extract (62.24 mu g GAE/mg) and chloroform extract (87.72 mu g QE/mg) respectively. The highest DPPH radical scavenging activity was detected in ethyl acetate extracts. Antimicrobial and antifungal activities were investigated by MIC method. The inhibitory activities of the extracts on the acetyl cholinesterase enzyme were investigated. Liquid chromatography (LC) tandem mass spectrometry LC-MS/MS was used to determine the phenolic component content of extracts. Thirty-one different components were identified in the analyses and their amounts were measured. Practical applications Scilla mesopotamica speta is an endemic and medicinal plant. It was determined that the extracts of this plant had a very rich content in terms of phenolic compounds, especially caffeic and ferulic acids. However, this plant was remarkable for its antioxidant, anticholinesterase, and antimicrobial activities. Considering the strong antioxidant, antimicrobial, and enzyme inhibition activities of the Scilla mesopotamica speta it can be suggested as a source of anticancer, antimicrobial, and antiviral drugs.Article Gümüş Nano Materyallerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktiviteleri(2021) Aktepe, NecmettinGümüş nano materyallerin kullanım alanları oldukça yaygındır. Farklı sentezleme yöntemleri ile elde edilebilirler. Bu çalışmada, gümüş nano materyaller (AgNP’ler) biyosentez yöntemi kullanılarak ekonomik ve basit bir şekilde elde edildi. Sentez sonucunda elde edilen AgNP’ler UV-visiblespektrofotometre (UV-Vis.), Taramalı Elektron Mikroskobu (SEM), X- Işınımı Kırınımı Difraktrometresi (XRD), Fourier dönüşümü kızılötesi spektroskopisi (FTIR), Zeta potansiyeli cihazları kullanılarak karakterize edildi. AgNP’lerin 426.66 nm dalga boyunda maksimum absorbansa sahip oldukları, küresel morfolojik yapı sergiledikleri, 17.68 nm kristal nano boyut ve yüzey yüklerinin -20.9 mV zeta potansiyeli dağılımı gösterdiği değerlendirildi. Patojen mikroorganizmalar üzerinde Minumum İnhibisyon Konsantrasyonları (MİK) 0.03-0.5 mg/L olarak mikrodilusyon yöntemi kullanılarak tespit edildi.Article Citation - WoS: 18Citation - Scopus: 14In-Vitro Antioxidant, Cytotoxic, Cholinesterase Inhibitory Activities and Anti-Genotoxic Effects of Hypericum Retusum Aucher Flowers, Fruits and Seeds Methanol Extracts in Human Mononuclear Leukocytes(Iranian Journal of Pharmaceutical Research, 2017) Keskin, C.; Keskin, Cumali; Aktepe, N.; Aktepe, Necmettin; Yükselten, Y.; Asuman,; Boğa, M.; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 09.01. Department of Nursing / Hemşirelik Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu ÜniversitesiThe present study investigates the antioxidant, anticancer, anticholinesterase, anti-genotoxic activities and phenolic contents of flower, fruit and seed methanol extracts of Hypericum retusum AUCHER. The amounts of protocatechuic acid, catechin, caffeic acid and syringic acid in methanol extracts were determined by HPLC. Total phenolic content of H. retusum seed extract was found more than fruit and flower extracts. The DPPH free radical scavenging activity of flower and seed methanol extracts showed close activity versus BHT as control. Among three extracts of H. retusum only flower methanol extract was exhibited considerable cytotoxic activities against to HeLa and NRK-52E cell lines. Moreover, seed methanol extract showed both acetyl and butyrl-cholinesterase inhibitory activity. The highest anti-genotoxic effects were seen 25 and 50 μg/mL concentrations. In this study, the extracts showed a strong antioxidant and anti-genotoxic effect. The seed extract was more efficient- than extracts of fruit and flowers. Our results suggest that the antioxidant and anti-genotoxic effects of extracts depend on their phenolic contents. Further studies should evaluate the in-vitro and in-vivo the benefits of H. retusum seed methanol extracts. © 2017 by School of Pharmacy.Article Citation - WoS: 13Citation - Scopus: 13Fast and Low-Cost Biosynthesis of AgNPs with Almond Leaves: Medical Applications with Biocompatible Structures(Progress in Nutrition, 2021) Aktepe, Necmettin; Baran, AyşeIn this study, silver nanoparticles (AgNPs) were obtained in a low cost, easy and simple way by using the leaf extract of Prunus dulcis L. (almond tree) growing in the Mardin region. Characterization of AgNPs obtained by biosynthesis: Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffractometer (XRD), UV-visible spectrophotometer (UV-Vis.), Scanning Electron Microscope (SEM), Transmission Electron Microscope(TEM), Zeta potential and Zeta size analysis was done through data. It was determined that AgNPs have maximum absorbance at 443 nm wavelength, they exhibit 14.67 crystal nano size, -19.9 mV zeta potential in spherical appearance. The Minimum Inhibition Concentration (MIC) of the obtained AgNPs was determined by using microdilution method on the growth of pathogen strains.Article Green synthesis and antimicrobial effects of silver nanoparticles by pumpkin cucurbita maxima fruit fiber(Medicine Science, 2022) Aktepe, Necmettin; Baran, AyşeIn the present study, for the first time, green synthesis of silver nanoparticles was carried out with the extract obtained from the fibrous structure in the pumpkin Cucurbita maxima (CM) fruit. The presence of silver nanoparticles formed by the reduction of Ag+ ions was determined with the maximum absorbance values of 442.89 nm in wavelength scans made by UV-Vis spectroscopy analysis. The functional groups of the phytochemicals involved in the reduction were examined by Fourier-transform infrared (FT-IR) spectroscopy. For the morphology of the synthesized AgNPs, they were found to be uniform in spherical appearance using atomic power microscopy (AFM), transmission electron microscopy (TEM), and field emission scanning electron microscopy (FE-SEM) images. The surface charges of AgNPs were determined to be -16.4 mV by Zeta potential analysis. Their crystal structures and nano-sizes were evaluated by X-ray diffraction (XRD) data to be in cubic pattern, and their size was 21.20 nm using the Debye-Scherrer equation. AgNPs (CM-AgNPs) synthesized via CM provided growth suppression at concentrations of 1.00-0.12, 2.00-1.00, and 0.50 µg/mL on pathogen gram-positive, gram-negative strains and the fungus Candida albicans, respectively. These concentrations were effective against antibiotics and silver solution at a very low concentration.Article The protective effects of different parts of hypericum perforatum extracts on human mononuclear leukocytes in hydrogen peroxide-induced DNA damage and their phenolic contents(Medicine Science, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Atalar, Mehmet Nuri; Baran, Mehmet FıratOxidative stress is the state of the formation of some pathophysiological condition with the excessive increase of the normal amount of free radicals in the organism. In this study, the in vivo genotoxic and antigenotoxic effects of methanol and water extract and phenolic content of Hypericum perforatum flower, fruit, and seed methanol extracts were analyzed. HPLC was used to evaluate the quantities of 3,4-Dihydroxybenzoic acid, syringic acid, hydroxycinnamic acid, O-coumaric acid, caffeic acid, and catechin in the methanol extracts. The alkaline comet test was used to assess the DNA damage and protective effects of H. perforatum flower fruit, seed methanol, and water extract on human mononuclear leukocytes. The amounts of catechin and caffeic acid in seed methanol extract were found as quite high when compared to other extracts. The highest protective effects were seen at 10 and 50μg/ml concentrations of seed methanol extract. The optimum doses of fruit, flower, and seed extracts obtained from H. perforatum neutralized the genotoxic effect. This effect is stronger in seed methanol extract than other extracts. We suggest that more research is needed to evaluate the effects of H. perforatum phytochemicals in vitro and in vivo.Article Citation - WoS: 1Removal of Heavy Metals in Water by Biosorption Method Using Three Different Bacillus sp-derived Biosorbents(Kahramanmaraş Sütçü İmam Üniversitesi, 2022) Baran, Mehmet Fırat; Düz, Zahir; Baran, Ayşe; Keskin, Cumali; Aktepe, NecmettinWater is an important part of the ecosystem for life. With industrialization, pollution in water resources has reached a worrying level. Water pollution due to heavy metals and their increasing concentrations have caused researchers to increase their interest in the subject due to the damage they cause to water ecosystems. It requires serious cost and time to eliminate the pollution caused by heavy metals in water. In recent, years, the use of biosorption method using bacteria to remove heavy metals in water has become widespread. The main reason why this method is preferred is that gram-positive bacteria have a thick peptidoglycan layer on the cell wall and increases the adsorption capacity. In this study. in drinking, waste, river water and artificially prepared samples, batch method of heavy metal biosorption and biosorption competition in multiple prepared heavy metal solutions were investigated. For these processes. Bacillus licheniformth sp. Bacillus subtilis sp. and Bacillus subtilis (ATCC 6051) strains were used as a biosorbent. Biosorbtion of Cd (II), Cu (H), Pb (II), Fe(II), Ni (II) and Zn (II) metals from waters with these biosorbents at different pHs at. 25 degrees C with 0.25 mg LI It was investigated. Surface morphological structures of biosorbents were evaluated using SEM images and element compositions were evaluated using EDAX profile. Element content was determined using ICP-OES. It was found that heavy metal ions were removed up to 98% with maximum biosorption at pH 6.0.
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