Profile URL: https://hdl.handle.net/20.500.12514/7098
Job Title:Doktor Öğretim Üyesi
Email Address:songulcetik@gmail.com
Main Affiliation:Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
ORCID:
0000-0002-7855-5343
0000-0002-7855-5343Scopus ID:
57204657759
57204657759YÖK Akademik: 436DB03AA14B155F
Google Scholar:
8PW61PUAAAAJ
8PW61PUAAAAJWeb of Science ID:
AAI-2285-2019
AAI-2285-2019Name Variants:
Songul Cetik Cetik, Songul
39 results
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Article Citation - WoS: 75Citation - Scopus: 81Cyclophosphamide induced oxidative stress, lipid per oxidation, apoptosis and histopathological changes in rats: Protective role of boron(Elsevier, 2020-12-01) Cengiz, Mustafa; Şahintürk, Varol; Cetik Yildiz, Songul; Kurcanay Şahin, İlknur; Bilici, Namık; Onur Yaman, Suzan; Altuner, Yılmaz; Appak-Baskoy, Sıla; Ayhanci, Adnan; Yildiz, Songul Cetik; Sahin, Ilknur Kulcanay; Yaman, Suzan OnurBackground Cyclophosphamide (CP) is an alkylating chemotherapeutic drug used in the treatment of many types of cancer. However, as with other chemotherapeutic drugs, the use of CP is limited by the damage to healthy tissues such as testes, bladder and liver as well as cancerous tissue. Boron (B) is a trace element with many biological properties such as antioxidant, anti-apoptotic and anti-lipid per oxidation. Methods This current study aims to determine protective effects of B on CP induced testicular toxicity. The rats were divided into 4 groups (control, CP, B and B plus CP groups). The testes of experimental animals were taken for histological, apoptotic markers and biochemical analysis. Results The damage to some seminifer tubules, loss of typical appearance, thinning of seminifer epithelium and relative enlargement of the tubule lumen were watched in testis of the group that administrated CP. Moreover, Bcl-2, TAC and GSH levels decreased while TOC, OSI, MDA, Bax and Caspase-3 levels increased. On the other hand, pretreatment limited to B in the B plus CP group, testicular tissue improved. In addition, Bcl-2, GSH, TAC levels increased, Bax, MDA, TOC, OSI and caspase-3 levels decreased. Conclusion B significantly reduced testicular lipid per-oxidation and strengthened antioxidant defenses. Our results showed that pre-treatment B can protect rat testis against CP-induced testicular damage owing to its anti-lipid per oxidation, anti-oxidant and anti-apoptotic properties.Article Myeloprotective and hematoprotective role of kefir on cyclophosphamide toxicity in rats(2021) Gözüoğlu, Gülay; Cetik Yildiz, SongulAbstract Aim: Kefir is a probiotic and prebiotic beverage produced from milk and kefir grains containing a mixture of bacteria and yeast. Drugs like cyclophosphamide (CPx) that are used for cancer chemotherapy are generally limited due to numerous unwanted side-effects such as multiple organ toxicity. For this purpose, the cell-protective effects of kefir, a natural probiotic known for its antitumor and antioxidant properties, on CPx-induced hemotoxicity and myelotoxicity were investigated in this study. Methods: Group 1 (control, 0.5 ml SF). Group 2 were administered a single dose of 150mg/kg CPx. Group 3 and 5 were given 5 and 10mg/kg kefir. Group 4 and 6 were given 5 and 10mg/kg kefir+150mg/kg CPx. While kefir was administered to the rats by gavage method for 12 days, CPx was administered as single-dose on the 12th day. Results: The DPPH results showed that kefir possesses high antioxidant activity. It was observed that the leukocytes, thrombocytes, erythrocytes, hemoglobin, hematocrit and bone marrow nucleated cell levels decreased in the group that was administered only CPx, and increased relatively in the groups that were administered CPx+kefir, drawing close to the control. Conclusion: The results of the present study showed that kefir had antioxidant and cytoprotective activity, protecting blood and bone marrow cells against CPx-induced damage.Article Citation - WoS: 9Citation - Scopus: 13The Protection Afforded by Kefir Against Cyclophosphamide Induced Testicular Toxicity in Rats by Oxidant Antioxidant and Histopathological Evaluations(Nature Portfolio, 2024-08-09) Yildiz, Songul Cetik; Demir, Cemil; Cengiz, Mustafa; Irmak, Halit; Cengiz, Betul Peker; Ayhanci, Adnan; Peker Cengiz, Betul; Cetik Yildiz, SongulCyclophosphamide (CTX) is the most commonly used effective alkylating drug in cancer treatment, but its use is restricted because its toxic side effect causes testicular toxicity. CTX disrupts the tissue redox and antioxidant balance and the resulting tissue damage causes oxidative stress. In our study based on this problem, kefir against CTX-induced oxidative stress and testicular toxicity were investigated. Rats were divided into 6 groups: control, 150 mg/kg CTX, 5 and 10 mg/kg kefir, 5 and 10 mg/kg kefir + 150 CTX. While the fermented kefirs were mixed and given to the rats for 12 days, CTX was given as a single dose on the 12th day of the experiment. Testis was scored according to spermatid density, giant cell formation, cells shed into tubules, maturation disorder, and atrophy. According to our biochemical findings, the high levels of total oxidant status (TOS), and the low levels of total antioxidant status (TAS) in the CTX group, which are oxidative stress markers, indicate the toxic effect of CTX, while the decrease in TOS levels and the increase in TAS levels in the kefir groups indicate the protective effect of kefir. In the CTX-administered group, tubules with impaired maturation and no spermatids were observed in the transverse section of the testicle, while in the kefir groups, the presence of near-normal tubule structures and tubule lumens despite CTX showed the protective effect of kefir. In our study, it was observed that kefir had a protective and curative effect on CTX-induced toxicity and oxidative stress and could be a strong protector.Master Thesis Sıçanlarda siklofosfamid ile oluşturulmuş hematoksisite ve myelotoksisite üzerine kefirin olası hücre koruyucu etkileri(Mardin Artuklu Üniversitesi, 2021) Gözüoğlu, Gülay; Yıldız, Songül ÇetikKefir, geleneksel olarak süt ve kefir mayasının fermentasyonu ile elde edilen ve egzotik tada sahip probiyotik/prebiyotik bir süt ürünüdür. Siklofosfamid (CPX) gibi kanser kemoterapisi için sıklıkla tercih edilen ilaçların gerekli ve etkin dozlarda kullanımı, genellikle, istenmeyen şiddetli akut toksik yan etkiler yüzünden sınırlıdır. Bu nedenle bu gibi ilaçların kemoterapötik etkinliklerini korurken yan etkilerini en aza indirecek yeni stratejilere ihtiyaç duyulmaktadır. Bu amaçla antitümör ve antioksidan özellikleri ile iyi bilinen, doğal bir probiyotik olan kefirin CPX nedenli hemotoksisite ve myelotoksisite üzerine hücre koruyucu etkileri araştırılmıştır. Bu deneysel çalışmada kullanılan 42 sıçan her grupta 7şer tane olmak üzere 6 gruba ayrıldı. Grup 1 (kontrol), Grup 2; 150 mg/kg CPX, Grup 3; 5 mg/kg kefir, Grup 4; 5 mg/kg kefir +150 mg/kg CPX, Grup 5; l0 mg/kg kefir, Grup 6; l0 mg/kg kefir + 150 mg/kg CPX olarak oluşturuldu. Kefir sıçanlara 12 gün boyunca gavaj yöntemi ile mg/kg/v.a olarak verilirken CPX ise tek doz ve ip./ v.a olarak deneyin son günü olan 12. gün verildi. Deney bitiminde yani 13. gün tüm sıçanlar disekte edilerek kan ve kemik iliği örnekleri alındı. Ayrıca kefirin antioksidan aktivitesi, asitlik ve pH'ı değerlendirildi. Elde edilen sonuç, kefirin güçlü antioksidan aktiviteye sahip olduğunu göstermiştir. Ayrıca lökosit, trombosit, eritrosit, hemoglobin, hematokrit ve kemik iliği çekirdekli hücre düzeylerinin sadece CPX verilen grupta düştüğünü, CPX+kefir verilen gruplarda ise sadece CPX verilen gruba kıyasla bu değerlerin yükseldiği ve kontrole yaklaştığı görülmüştür. Sonuç olarak çalışmamız kefirin antioksidan ve sitoprotektif özellik göstererek kan ve kemik iliği hücrelerini CPX hasarına karşı koruduğunu göstermiştir.Article Citation - WoS: 13Citation - Scopus: 13Protective properties of kefir on burn wounds of mice that were infected with S. aureus, P. auroginasa and E. coli(Cellular and Molecular Biology (CMB), 2019-09-30) Çetik Yıldız, Songül; Demir, Cemil; Cengiz, Mustafa; Ayhanci, Adnan; Yildiz, Songul CetikBurns and burn wounds are very sensitive to infections and cause a large amount of death worldwide. Although burn wound is sterile at the beginning, because of the risk factors such as prolonged hospital stay, immune suppression and burn affecting large surface area, colonisation with Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli occur. For the burn therapy, one of the most important ways is to control bacterial infections. A probiotic fermented milk product kefir has antioxidant, antimicrobial, antiinflammatory, anticancer and various health promoting features. This study aims to examine possible protective properties of kefir which was used on the burn wounds that were infected with S. aureus, P. auroginasa and E. coli. Swiss albino / Balb-c mice were seperated into four groups: (1) used as control group, (2) second-degree burn model+ burn wounds were infected with P.aeruginosa + S.aureus + E.coli, (3) second-burn wounds were treated with sterile pads dressed with kefir and (4) second-degree burn+burn wounds were infected with P. aeruginosa + S.aureus +E.coli before being treated with sterile pads dressed with kefir. The serum biochemical results verified the histopathological results and our findings showed that kefir is an effective product with cell-protecting properties.Article Presence of biofilm and adhesin genes in Staphylococcus aureus strains taken from chronic wound infections and their genotypic and phenotypic antimicrobial sensitivity patterns(2020) Demir, Cemil; Demirci, Mehmet; Yigin, Akin; Tokman, Hrisi Bahar; Cetik Yildiz, SongulThe purpose of this research was to examine biofilm (icaA, icaD and bap) and adhesin (clfA, fnbA, cna) genes, and also assess the genotypic and phenotypic antimicrobial resistance patterns of Staphylococcus aureus strains taken from wound specimens in Mardin, Turkey. A total of 220 wound specimens were investigated. The biofilm forming ability and resistance pattern for eleven antimicrobial agents were investigated by conventional and multiplex PCR methods. S. aureus were taken from 112 (50.9%) of 220 wound specimens. Moreover, biofilm production was found in 79 (70.5%) of the 112 S. aureus isolates. 97 (86.6%) strains of all isolates were positive for icaA and icaD, and 15 (13.4%) for bap. The adhesin genes, cna, fnbA and clfA were detected in 98 (87.5%), 87 (77.7%), and 75 (66.9%) strains, respectively. The numbers of MSSA and MRSA bearing antimicrobial resistance genes were 19 (16.96%) and 32 (28.57%) for blaZ, 9 (8.04%) and 17 (15.18%) for tetK, 6 (5.36%) and 14 (12.5%) for ermC, 2 (1.79%) and 7 (6.25%) for tetM, 0 (0%) and 5 (4.46%) for mecA, 2 (1.79%) and 4 (3.57%) for ermA, 1 (0.89%) and 2 (1.79%) for both tetK and tetM, respectively. Our findings indicate that multiplex PCR is a suitable way for identifying biofilm and adhesin producing S. aureus. Our data also provided a country-wide oversight of the S. aureus antimicrobial resistance gene profiles for the properly therapy of patients and to control the spreading of the resistance genes.Article Citation - WoS: 12Citation - Scopus: 12In Vitro Antitumor and Antioxidant Capacity as Well as Ameliorative Effects of Fermented Kefir on Cyclophosphamide-Induced Toxicity on Cardiac and Hepatic Tissues in Rats(Mdpi, 2024-05-28) Yildiz, Songul Cetik; Demir, Cemil; Cengiz, Mustafa; Irmak, Halit; Cengiz, Betul Peker; Ayhanci, Adnan; Cetik Yildiz, SongulFermented prebiotic and probiotic products with kefir are very important to slow down and prevent the growth of tumors and to treat cancer by stimulating the immune response against tumor cells. Cyclophosphamide (CPx) is widely preferred in cancer treatment but its effectiveness in high doses is restricted because of its side effects. The aim of this study was to investigate the protective effects of kefir against CPx-induced heart and liver toxicity. In an experiment, 42 Wistar albino rats were divided into six treatment groups: the control (Group 1), the group receiving 150 mg/kg CPx (Group 2), the groups receiving 5 and 10 mg/kg kefir (Groups 3 and 4) and the groups receiving 5 and 10 mg/kg kefir + CPx (Group 5 and 6). Fermented kefirs obtained on different days by traditional methods were mixed and given by gavage for 12 days, while a single dose of CPx was administered intraperitoneally (i.p.) on the 12th day of the experiment. It was observed that alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatinine kinase-MB (CK-MB), ischemia modified albumin (IMA) and Troponin I values, which indicate oxidative stress, increased in the CPx-administered group, and this level approached that of the control in the CPx + kefir groups. Likewise, as a result of the kefir, the rats' CPx-induced histopathological symptoms were reduced, and their heart and liver tissue were significantly improved. In conclusion, it was observed that kefir had a cytoprotective effect against CPx-induced oxidative stress, hepatotoxicity and cardiotoxicity, bringing their biochemical parameters closer to those of the control by suppressing oxidative stress and reducing tissue damage.Article Citation - WoS: 3Citation - Scopus: 2Examination of the effects of kefir on healing factors in a mice burn model infected with E.coli, S.aureus and P.aeruginosa using qRT-PCR(Elsevier, 2023-03-01) Çetik Yıldız, Songül; Demir, Cemil; Ayhancı, Adnan; Yildiz, Songul CetikBurn areas are susceptible to bacterial growth and infections, particularly in cases with lengthy periods of hospital stay. Burn wound healing, which involves various molecular and cellular mechanisms, continues to be a significant problem. Growth factors and cytokines play an active and vital role in wound healing. In the present study, the effects of kefir on wound healing in a 2nd-degree mouse burn model infected with e.coli, s.aureus and p.aeruginosa were investigated in vitro. In order to clarify the effects of kefir in the wound healing process, the macroscopic changes in kefir-applied scar tissue as well as wound depth and width were examined and IL-1α, IL-1β, IL-6, IL-8, IL-10 and TNF-α, VEGF, TGF-β protein levels were determined using the qRT-PCR method. The findings of the present study show that kefir has a positive impact on the factors playing a role in wound healing and accelerates the healing process.Article Hematoprotective Effect of N(G)-Nitro-L-Arginine Methyl Ester and Lycopene on Hematoxicity in Trinitrobenzene Sulfonic Acid–Induced Colitis in Rat(2015) Cetik, Songul; Ayhanci, Adnan; Altuner, Yilmaz; Musmul, Ahmet; Sahinturk, Varol; Yıldız, Songül ÇetikAnemia is a neglected manifestation of inflammatory bowel disease (IBD) although it is commonly observed in IBD patients. Attempts to overcome anemia in IBD would help not only general well being of the patients but also minimize disease consequences. In this experimental study, the possible hematoprotective effects of lycopene and NG–nitro-L-arginine methyl ester (L-NAME) on colitis induced by TNBS were analyzed. 112 rats were assigned to 16 groups; control group, intrarectal 120 mg/kg TNBS group, intraperitoneal 40 mg/kg L-NAME group, 1 mg/kg olive oil group, 5 and 10 mg/kg lycopene groups. Each experimental group was divided into 3 subgroups according to duration of treatment. On the very first day of treatment number of erythrocytes decreased in all groups except TNBS treated group whereas leukocyte numbers increased in all groups except TNBS treated group pointing out an inflammation. The number of platelets decreased in all study groups with the exception of TNBS group. On the second day, while erythrocyte and platelet numbers increased in all but not in TNBS group, leukocytes decreased in all the groups. On the third day, erythrocyte and platelet numbers increased in all groups except for the 10 mg/kg lycopene group. While the number of leukocytes decreased in the 10 mg/kg lycopene group, it remained the same in the other groups as those observed on the second day. These results show that lycopene could have effects on hemopoiesis as well as in prevention of anemia in IBD.Article Citation - WoS: 10Citation - Scopus: 5Cardioprotective Effects of Hypericum Triquetrifolium Turra. Against Cyclophosphamide Related Cardiotoxicity in Rats(Marmara Univ, 2018-07-02) Cetik Yildiz, Songul; Keskin, Cumali; Sahinturk, Varol; Ayhanci, AdnanCyclophosphamide (CYP) is commonly used as anticancer agent but its usage is limited by cardiotoxic side effects such as dose-dependent cardiac damage, morphologically defined necrosis and bleeding. Hypericum triquetrifolium Turra. (HT) shows anti-oxidative and anticarciogenic properties with its rich phenolic contents. The current study was designed to investigate the possible protective effect of HT on CYP-induced cardiotoxicity. Albino rats were randomly divided into 9 groups, each included 7 animals. Serum creatine kinase-MB (CK-MB), malondialdehyde (MDA), aspartate transaminase (AST), glutathione (GSH), total antioxidant (TAC) and total oxidant capacity (TOC) levels were investigated. Furthermore, the cardiac tissue samples were investigated histopatologically. While the levels of serum CK-MB, MDA, AST and TOC were high, the levels of serum GSH and TAC levels were low in the CYP groups. It was also observed that CYP-induced cardiotoxicity was dose dependent. In the treatment with CYP plus HT doses there was observed an essential decrease in the CYP cardiotoxicity; decreased cell damage and oxidative stress parameters and also increased GSH and TAC levels. Based on our findings, it can be proposed that HT seed methanol extract was a strong candidate in preventing the CYP-induced cardiotoxicity.