Çetik Yıldız, Songül

Çetik Yıldız, Songül

Name Variants

Songul Cetik
Cetik, Songul
Cetik Yildiz, Songul

Job Title

Doktor Öğretim Üyesi

Email Address

songulcetik@gmail.com

Main Affiliation

Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü

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Scholarly Output

17

Articles

17

Citation Count

0

Supervised Theses

0 Scholarly Output Search Results

Now showing 1 - 6 of 6

Investigation into the protective effects of Hypericum Triquetrifolium Turra seed against cyclophosphamide-induced testicular injury in Sprague Dawley rats
(Taylor & Francis Online, 2022) Çetik Yıldız, Songül; Keskin, Cumali; Keskin, Cumali; Şahintürk, Varol; Cengiz, Mustafa; Appak Başköy, Sıla; Ayhanci, Adnan; Akıncı, Gülsema
For centuries, Turkey has been a significant location here around 80 species of Hypericum with differing names widely occur, which is also known as Turkish folk medicine in treating some bacterial diseases as well as stomach and intestine inflammation. Recent studies have reported this herb family to contain numbers of bioactive compound contents. The study aims to investigate the protective effects of Hypericum triquetrifolium Turra (HT) upon oxidative stress and apoptosis in a rat model in which testes injury was induced by CP. The testicular injury was caused using CP (150 mg/kg). The rats were treated with a single dose (100 mg/kg) of methanol extract of HT to investigate various biochemical markers in the serum and plasma of blood samples apart from assessing the prognosis of CP-induced testicular damage. Added to that, histological analyses were performed to identify possible structural changes and apoptotic indicators, like Bax, Caspase-3, and Bcl-2. In CP Group, there was a rise in the levels of total oxidant status (TOS), malondialdehyde (MDA), oxidative stress index (OSI), Caspase-3, and Bax while superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), Bcl-2, and total antioxidant capacity (TAC) all decreased. Also, our histological analysis showed damaged testes. On the other hand, neither biochemical nor histological analysis showed testicular damage in HT Alone Group. In CP + HT Group, a significant number of the negatives changes due to CP were observed to have improved remarkably following an HT treatment. This study results suggest that HT could help improve CP-induced testicular injury thanks to its anti-oxidative and anti-apoptotic properties.
Hepato-preventive and anti-apoptotic role of boric acid against liver injury induced by cyclophosphamide
(Elsevier GmbH, 2019) Çetik Yıldız, Songül; Demir, Cemil; Demir, Cemil; Kulcanay Şahin, İlknur; Teksoy, Özgün; Ayhancı, Adnan
This study aims to examine cyclophosphamide (CP) exsposure associated toxicity on rat livers and the likely defensive effects of boric acid (BA). The rats used in this study were divided into four groups: control group, CP group, BA group, and BA + CP group. The present study was carried out using routine histological H&E stain, immunohistochemical stain caspase-3 as apoptotic marker, serum biochemical analysis for liver function markers (alanine transaminase (ALT), aspartate transaminase (AST) and alkalen phosphatase (ALP)), oxidative stress markers (total oxidant status (TOS), oxidative stress index (OSI) and total antioxidant capacity marker (TAC)). In the CP group, the levels of ALT, AST, ALP, TOS, OSI and caspase-3 increased whereas TAC levels decreased compared with the control group. In the BA + CP group, the levels of ALT, AST, ALP, TOS, OSI and caspase-3 decreased whereas TAC levels increased compared with the CP group. The histopathological evaluation of light microscope images and immunohistochemical caspase-3 activity in the BA + CP group were found to be decrease compared with those in the CP group. In conclusion, BA was successful in defending the liver against apoptosis and histopathological changes that are attributable to CP. © 2019 Elsevier GmbH
Protective properties of kefir on burn wounds of mice that were infected with S. aureus, P. auroginasa and E. coli
(Cellular and Molecular Biology (CMB), 2019) Çetik Yıldız, Songül; Demir, Cemil; Cengiz, Mustafa; Ayhanci, Adnan
Burns and burn wounds are very sensitive to infections and cause a large amount of death worldwide. Although burn wound is sterile at the beginning, because of the risk factors such as prolonged hospital stay, immune suppression and burn affecting large surface area, colonisation with Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli occur. For the burn therapy, one of the most important ways is to control bacterial infections. A probiotic fermented milk product kefir has antioxidant, antimicrobial, antiinflammatory, anticancer and various health promoting features. This study aims to examine possible protective properties of kefir which was used on the burn wounds that were infected with S. aureus, P. auroginasa and E. coli. Swiss albino / Balb-c mice were seperated into four groups: (1) used as control group, (2) second-degree burn model+ burn wounds were infected with P.aeruginosa + S.aureus + E.coli, (3) second-burn wounds were treated with sterile pads dressed with kefir and (4) second-degree burn+burn wounds were infected with P. aeruginosa + S.aureus +E.coli before being treated with sterile pads dressed with kefir. The serum biochemical results verified the histopathological results and our findings showed that kefir is an effective product with cell-protecting properties.
Examination of the effects of kefir on healing factors in a mice burn model infected with E.coli, S.aureus and P.aeruginosa using qRT-PCR
(Elsevier, 2023) Çetik Yıldız, Songül; Demir, Cemil; Ayhancı, Adnan
Burn areas are susceptible to bacterial growth and infections, particularly in cases with lengthy periods of hospital stay. Burn wound healing, which involves various molecular and cellular mechanisms, continues to be a significant problem. Growth factors and cytokines play an active and vital role in wound healing. In the present study, the effects of kefir on wound healing in a 2nd-degree mouse burn model infected with e.coli, s.aureus and p.aeruginosa were investigated in vitro. In order to clarify the effects of kefir in the wound healing process, the macroscopic changes in kefir-applied scar tissue as well as wound depth and width were examined and IL-1α, IL-1β, IL-6, IL-8, IL-10 and TNF-α, VEGF, TGF-β protein levels were determined using the qRT-PCR method. The findings of the present study show that kefir has a positive impact on the factors playing a role in wound healing and accelerates the healing process.
Cardioprotectiveeffectsof Hypericumtriquetrifoliumturra. Against Cyclophosphamide Related Cardiotoxicity in Rats
(2018) Çetik Yıldız, Songül; Keskin, Cumali; Ayhanci, Adnan; Şahintürk, Varol; Yıldız, Songul Cetık; Keskin, Cumali
Cyclophosphamide (CYP) is commonly used as anticancer agent but its usage is limited by cardiotoxic side effects such as dose-dependent cardiac damage, morphologically defined necrosis and bleeding. Hypericum triquetrifoliumTurra. (HT) shows anti-oxidative and anticarciogenic properties with its rich phenolic contents. The current study was designed to investigate the possible protective effect of HT on CYP-induced cardiotoxicity. Albino ratswererandomlydividedinto9groups,eachincluded7animals.Serumcreatinekinase-MB(CK-MB), malondialdehyde (MDA), aspartate transaminase (AST), glutathione (GSH), total antioxidant (TAC) and total oxidant capacity (TOC) levels were investigated. Furthermore, the cardiac tissue samples were investigated histopatologically. While the levels of serum CK-MB, MDA, AST and TOC were high, the levels of serum GSH and TAC levels were low in the CYP groups. It was also observed that CYP-induced cardiotoxicity was dose dependent. In the treatment with CYP plus HT doses there was observed an essential decrease in the CYP cardiotoxicity; decreased cell damage and oxidative stress parameters and also increased GSH and TAC levels. Based on our findings, it can be proposed that HT seed methanol extract was a strong candidate in preventing the CYP-induced cardiotoxicity.
A Histopathological, Immunohistochemical and Biochemical Investigation on the in Vitro Antioxidant, Myeloprotective, Hematoprotective and Hepatoprotective Effects of Hypericum Triquetrifolium Seed Extract Against Cyclophosphamide-Induced Toxicity
(Inst Tecnologia Parana, 2019) Keskin, Cumali; Yildiz, Songul Cetik; Keskin, Cumali; Çetik Yıldız, Songül; Sahinturk, Varol; Ayhanci, Adnan
The aim of this study was to investigate in vitro antioxidant properties and in vivo protective effects of the methanol extract of the Hypericum triquetrifolium Turra (HT) seed against acute hepatotoxicity, myelotoxicity and hematotoxicity in rats induced by cyclophosphamide (CP). In order to investigate in vivo protective effects of the HT extract on rat tissues, the rats were divided into nine groups. The toxic effects of CP and the protective effects of HT extract on nucleated cells that are produced by bone marrow, serum alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and oxidative stress index (OSI) levels were investigated biochemically. Additionally, liver tissue samples were examined for histopathological changes and apoptosis by Bcl-2, Bax and caspase-3 immunohistochemistry. The results of this study show that HT seed methanol extract has high total phenolic content (179.52 mu g GAE/mg) and antioxidant activity (87.48% in 500 mu g/mL concentration). CP administration caused hepatotoxicity, myelotoxicity and hematotoxicity in the rats. Whereas, the groups of rats that were injected with different concentrations of HT (25, 50 and 100 mg/kg) and CP (150 mg/kg) showed significant protective effects on bone marrow nucleated cells and important decreases on serum ALT, ALP, LDH and OSI levels were observed when compared with the CP injected group.