Tarhan, Tuba

Tarhan, Tuba

Name Variants

Tural; Tarhan; Tural;, Servet; Tuba; Bilsen;
Tural; Tarhan; Tural, Bilsen; Tuba; Servet
Tarhan; Tural; Tural; Topal;, Tuba; Bilsen; Servet; Giray
Tarhan, T.

Job Title

Doçent

Main Affiliation

Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü

Sustainable Development Goals Report Points

SDG data could not be loaded because of an error. Please refresh the page or try again later.

Scholarly Output

39

Articles

15

Citation Count

0

Supervised Theses

1 Scholarly Output Search Results

Now showing 1 - 10 of 35

Synthesis of Drug Loaded Magnetic Nanocomposite and Investigation of Cytotoxic Properties
(2023) Tarhan, Tuba; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
This aims of study to develop drug delivery systems providing an alternative solution to cancer chemotherapy. Firstly, carboxymethyl dextran which has magnetic properties (MD), was synthesized as stated in the literature.1-3 Its structure was elucidated with various analytical devices. Then, loading studies were carried out on the nanocomposite with topotecan (TP) (in the range of 0.2-40 µM in phosphate buffer at pH 5) as an anticancer drug. The cytotoxic properties of MD, TP, drug loaded MD (MD-TP), and free TP were studied on human prostate cancer cell (Du145) and human healthy prostate cell (PNT1A) line. The synthesized MD and MD-TP nanocomposites were characterized by different analytical devices such as FTIR, XPS, UV-VIS, VSM, TEM, SEM and DLS. According to the FTIR results, the presence of peaks belonging to functional groups in the expected places indicates that MD was successfully synthesized and TP was successfully loaded on nanocomposite. According to TEM image, the MD particles showed a spherical structure, and also, the particle size was measured as 9 ± 1.8 nm. The SEM image of MD indicated that the nanoparticles were aggregated. Furthermore, after the loading TP, the brightly colored particles of TP were observed on some parts of the surface of MD. Moreover, DLS measurements of the nanoparticles were taken before and after loading the drug. Due to high agglomeration of the magnetic nanoparticle, it showed polydispersity. In addition, cytotoxicity effects of drug loaded nanocomposites on Du145 and PNT1A cell lines were investigated and the results were found to be statistically significant. The topotecan loaded MD-TP nanocomposite have shown cell viability to be reduced by up to 20 %. It has been shown that synthesized polymer coated superparamagnetic nanocomposites with these properties can be used in drug targeting studies.
Synthesis and Characterization Magnetic Nanocomposites as Chemotherapy Agents
(2018) Tarhan, Tuba; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
One of the most imp cancer cells as a side effect1,2. Studies on controlled drug release systems have increased with the aim of finding solutions to these problems3 . Controlled drug delivery systems at the nanoscale enable tumor targeting. Among the nanocarriers, biopolymer coated superparamagnetic nanoparticles have the ability to target at the desired region with an externally applied magnetic field. In this way, the drug can be safely delivery to the targeted area.3 The purpose of this study is, suitable for carrying the mentioned therapeutic agents, to have suitable magnetic, chemical and physical properties for use in biomedical applications, magnetic targeting, synthesis and characterization of branched polymeric materials with carboxyl groups4,5,6 . Biocompatible and biodegradable polymers is used as Chitosan. In addition, different derivatives of these polymers were synthesized and characterized by FTIR, TEM, SEM, EDX, DLS, XPS and VSM. As a results obtained that biocompatible and biodegradable magnetic nanocomposites were of the desired size and superparamagnetism, It has been shown that synthesized polymer coated superparamagnetic nanocomposites with these properties can be used in drug targeting studies and it is hoped that this type of work will contribute to future studies.
FABRICATION AND EVALUATION OF POLYDOPAMINE (PDA) COATED HEXAGONAL BORON NITRIDE (HBN) MODIFIED POLYDIMETHYLSILOXANE (PDMS) 3D POROUS SCAFFOLD FOR IMPROVE OSSEOINTEGRATION
(2020) Tarhan, Tuba; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
ABSTRACT: Purpose: Our goal is to enhance poor performance in supporting cell growth and proliferation in osseointegration. Herein, we fabricated a new scaffold material which was Polydimethylsiloxane (PDMS) based 3D structure, biocompatibility, cytocompatibility, and porous. Materials and Methods: In this study, the scaffold was fabricated as polydopamine (PDA) coated hexagonal boron nitride (hBN) embedded in PDMS. First, 3D porous structure PDMS was prepared using two agents of PDMS with the salt scaffold method. In the second step, the scaffold was prepared with PDA coated hBN was embedded in PDMS. Nowadays, the application of PDA as a superior surface modifier in multifunctional biomaterials is drawing tremendous interests in bone tissue scaffolds to promote the osseointegration for bone regeneration. The hBN was used to increase the durability of PDMS. Because, it possesses preeminent physical and chemical properties such as high chemical stability, temperature-resistant, high thermal conductivity, low density, good mechanical strength, anti oxidation ability, and biocompatibility. To perfectly the cells adhere to the surface, the hBN was coated with PDA. In the third step, the prepared scaffold was mineralized for the proliferation of cells. Results: The prepared PDMS-based 3D scaffolds were characterized by Raman and Fourier transform infrared (FT-IR) spectroscopies. The structure of morphology was studied using the scanning electron microscope (SEM). The compression and tensile stress as mechanical properties of the scaffolds were evaluated for the PDMS, PDMS- hBN-PDA, and its mineralized form PDMS- hBN-PDA-Min. The results of the tensile strength scaffolds of PDMS-hBN-PDA-Dry and PDMS-hBN-PDA-Wet were calculated 588.4 kPa and 614.7 kPa, respectively. However, the tensile strength of 3D PDMS-Dry and PDMS-Wet were calculated 285.3 kPa and 423.2 kPa, respectively. The tensile strength value of PDMS hBN-PDA-Dry was 53.6 % higher than that of 3D PDMS-Dry scaffold and also, elongation at break value of PDMS-hBN-PDA-Dry was 16.7 % higher than that of pristine PDMS-Dry scaffold. The contact angles were measured for the PDMS, PDMS- hBN-PDA, and PDMS- hBN-PDA-Min. scaffolds with deionized water droplets. The average contact angles (CAs) other mean surface wettability is a very important parameter for cell adhesion performance. The CAs were measured for the PDMS, PDMS hBN-PDA, and its mineralized form PDMS- hBN-PDA-Min scaffolds with deionized water droplets. The CAs of the PDMS was measured 87.6° ± 1.15 and after hBN-PDA was dispersed in PDMS its decreased around 72.1° ± 1.69. This lower CA was related to PDA containing the hydrophilic groups such as catechol, amide, and imide groups. Furthermore, after the mineralization process, the CA was decrease than before and it was found around 71.3° ± 1.20. This is because the catechol group of PDA was capturing some cations such as Ca2+ and K+ . The literature shows that the CA of scaffolds is at around 65°–70° the appropriate values for the cell adhesion and proliferation. The thermal stability and degradation mechanism of scaffolds were investigated using Thermogravimetric analysis (TGA). According to the results, while embedding hBN in PDMS showed higher resistance to temperature than pristine PDMS, the mineralization process showed the same curve as PDMS. Conclusion: In conclusion, PDMS is routinely used as a biomedical implant material and for fundamental cellular studies and has been confirmed as a biocompatible material. Moreover, the hBN used in the fabrication of scaffold is a novel nanostructure it has excellent properties such as high mechanical strengths, large surface area, and outstanding biocompatibilities. The application of PDA as a superior surface modifier in multifunctional biomaterials will be drawing tremendous interests in bone tissue scaffolds to promote the osteointegration for bone regeneration. Considering all these features and the results of characterization studies, we foresee that the prepared PDMS-hBN-PDA can be used as a perfect scaffold material in the future for cell adhesion and proliferation in osseointegration. Besides, a new 3D scaffold model based on PDMS was designed for bone tissue. Key Words: Bone repairing materials, Polydimethylsiloxane, Hexagonal boron nitride nanoparticles, dopamine, Scaffold.
Kanser Araştırmalarına Yönelik Manyetik O-Karboksimetil Kitosan Nanopartiküllerin Sentezlenmesi, Karakterizasyonu, İrinotekan yüklenmesi ve Glioblastoma Multiforme (Beyin Tümörü) Hücre Hatları Üzerine Sitotoksisite Değerlendirilmesi
(2020) Tarhan, Tuba; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Kanser basit bir ifadeyle kontrolsüz hücre çoğalması olarak tanımlanabilir. Kelime anlamı olarak kanser, bir organ veya dokudaki hücrelerin düzensiz olarak bölünüp çoğalmasıyla beliren kötü urlara denir. Kanser 100'den fazla hastalık grubunu içerir. Çok çeşitli kanser tipleri olmasına rağmen, hepsi anormal hücrelerin kontrol dışı çoğalması ile başlar. Tedavi edilmez ise ciddi rahatsızlıklara, hatta ölüme dahi neden olabilir (Türkiye Halk Sağlığı Kurumu, Kanser Daire Başkanlığı). Dünya istatistiklerinde her yıl kansere yakalanan kişi sayısının 12,7 milyon olduğu tahmin edilmekte ve bu sayının 2030 yılında 21 milyona yükselmesi beklenmektedir. Farklı ülkelerde farklı kanser türlerinin oranları yüksek olsa da dünyada ölüm nedenlerinin başında kanser gelmektedir (World Cancer Research Fund, 2013). Çok yaygın olarak görülen ve ölüm oranları yüksek olan bu hastalıkta genelde uygulanan tedavi yöntemleri kemoterapi, radyoterapi ve ameliyat ile sınırlıdır. Bu yöntemler yan etkisi çok olan invazif yöntemlerdir. Kanser tedavisi için en çok kullanılan yöntemlerden biri kemoterapi yöntemidir. Günümüzde kanser kemoterapisinin en önemli sorunlarından biri, kullanılan anti-kanser ilaçlarının kanserli hücreyi ayırt edici özelliğe sahip olmamaları ve yan etki olarak sağlıklı hücrelerin üzerinde de toksik etki göstermeleridir. Ayrıca tedavi edici doz konsantrasyonunu sağlamak için vücuda yüksek dozda ilaç verilmesi bununla birlikte ciddi yan etkilerin ve sistemik toksisitenin ortaya çıkmasıdır. Bu tür sorunların önüne geçebilmek için, nanoteknolojideki hızlı ilerleme, diğer alanlar ile birlikte sağlık alanında da önemli gelişmeleri beraberinde getirmiştir. Nanotıp (ya da nanoilaç) bir hastalığın tedavisi ya da hasarlı bir dokunun onarılması için moleküler düzeyde yüksek özgünlük gösteren bir medikal uygulama olarak tanımlanmıştır. Son zamanlarda ilaç endüstrisinde, teşhis ve tedavilerde, görüntüleme tekniklerinde, vücutta kontrollü ilaç salınımında ve ilacı hedef dokuya ulaştırmada, doku mühendisliğinde ve daha pek çok alanda nanoteknolojiden faydalanılmaktadır. Birçok farklı nanosistemden özellikle çok fonksiyonlu manyetik nanoparçacıklar (MNPs) bu alanda ilgi odağı olmaktadır. MNPs’ın manyetik alan altında yönlendirilebilme özelliği onları diğer nanoparçacıklara göre daha avantajlı kılmaktadır. Yeni nesil tedavi yöntemlerinde sağlıklı hücrelere zarar vermeden ilacı hedef bölgeye ulaştırma ve etkin bir tedavi için özellikle MNPs’ın kullanımları ve avantajları araştırılmaktadır. Bu projenin özgün değeri, kanser kamoterapisinden kaynaklı problemlere çözüm bulmak amacı ile son yıllarda kontrollü ilaç salım sistemleri üzerine yapılan çalışmalara alternatif bir çözüm geliştirmektir. Nano boyuttaki kontrollü ilaç taşıma sistemleri tümör hedeflenmesine olanak sağlamaktır. Nano taşıyıcılar arasından, biyouyumlu polimer kaplı paramanyetik nanoparçacıklar dışardan uygulanan manyetik alan ile istenilen bölgeye hedeflenebilme özelliklerine sahiptirler. Bu sayede ilaç hedeflenen bölgeye güvenli bir şekilde taşınabilmektedir. Bu parçacıklar gerekli modifikasyonlarla ilaç yüklenmeye uygun ve manyetik alan ile istenilen bölgeye ulaştırılabilen parçacıklar haline getirilebilmektedirler. Ayrıca, etken maddenin dozunun azaltılabilmesine ve etken maddenin sadece hedef bölgeye dağılımıyla sınırlandırılmasına olanak sağlayabilmektedir. Bu yöntem ile geleneksel kamoterapinin aksine sadece tümörlü hücrelere müdahale edilebilir ve ilaçların sağlıklı hücrelere etkisi azaltılabilir. Bu proje kapsamında en yaygın kullanılan anti-kanser ilaçlardan Kamptotesin analoğları ile çalışılacaktır. Kamptotesin topoizomeraz I inhibitörü olarak görev yapmaktadır. Bu enzim hücre bölünmesi esnasında DNA replikasyonundan sorumlu enzimdir. Kamptotesin analogları yapısında α-hidroksi δ-lakton halkası bulundurmaktadır. Ayrıca fizyolojik pH’ta ve alkali ortamda lakton halkası hidroliz olup karboksilat forma dönüşmektedir, bu haliyle stabil formdan uzaklaşıp vücut için toksik bir hal almaktadır. Dolaysıyla çalışılması zor olan ilaçlar arasında olup bununla ilgili yapılan çalışmaların çoğu patentlidir. Projemizin amacı, anti-kanser ilaç olarak önemli bir yere sahip olan Kamptotesin analoğlarının lakton formunda kararlı kalmasını sağlayan koşulları tespit etmek ve daha önce bu anti-kanser ilaçlar ile çalışılmamış manyetik özellik gösteren biyouyumlu ve biyobozunur polimerik madde sentezleyip, bu polimer moleküllerine ilacın yüklenmesi, taşınması ve salımını çalışmaktır.
Citation - WoS: 0
Covalent immobilization of benzoylformate decarboxylase on magnetic solid support and its carboligation reactivity
(WILEY-BLACKWELL, 2014) Tarhan, Tuba; Tural, B.; Tural, S.; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
…
Citation - WoS: 55
Citation - Scopus: 61
Maltose Functionalized Magnetic Core/Shell Fe₃o_{4< Nanoparticles for an Efficient L-Asparaginase Immobilization}
(Elsevier, 2020) Tarhan, Tuba; Ulu, Ahmet; Saricam, Melike; Culha, Mustafa; Ates, Burhan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
In this study, maltose-functionalized magnetic core/shell nanoparticles (Fe3O4@Au NPs) as a promising carrier matrix for a simple and effective immobilization of L-asparaginase (L-ASNase) were prepared and characterized using imaging techniques including atomic force microscopy (AFM) and transmission electron microscopy (TEM), and vibrating sample magnetometry (VSM). The results indicate that the NPs are monodispersed with an average diameter of 10 nm and magnetization of 9.0 emu g(-1). Under the optimal conditions, 77.2 +/- 2.3% of the total L-ASNase was immobilized. It was found that the acid-base tolerance and thermal stability of immobilized L-ASNase were significantly improved in comparison to the free form of the enzyme in solution. For instance, while only 10% of the immobilized enzyme was lost its activity, the free form was lost its activity more than 50% after 3 h incubation at 55 degrees C. After 13 times recycling, the immobilized L-ASNase retained about 50% of its initial activity. Moreover, the free and immobilized L-ASNase maintained their initial activities about 25 and 64% after 28 days storage at 25 degrees C, respectively. Km value of immobilized L-ASNase decreased to 1.59 from 2.95 mM as an indication of increased enzyme affinity for the substrate. The results of this study suggest that the maltose-coated magnetic nanoparticles are excellent nanovehicles to carry enzymes for a range of industrial applications. (C) 2019 Elsevier B.V. All rights reserved.
Synthesis and Characterization of Hexagonal Boron Nitride (hBN) for an Effective Removal of Organic Dyes: Kinetic and Equilibrium Studies
(2020) Tarhan, Tuba; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
In this study, hBN nanostructure was synthesized from boric acid to remove organic dyes from aqueous solution. The characteristic peaks of hBN are obtained using Raman and Fourier Transform Infrared (FT-IR) spectroscopies. However, the morphology and particle size of hBN are determined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). During the studies, various key factors such as pH of the aqueous dye solution, initial dye concentration, adsorbent dose and contact time are investigated. Additionally, equilibrium isotherm and kinetic models are studied for the removal of anionic and cationic organic dye Metanyl Yellow (MY) and Victoria Blue B (VBB), respectively. Under optimal conditions, it was found that 42.6% Methanyl Yellow (MY) and 90% Victoria Blue B (VBB) were removed from aqueous solution using the hBN nanostructure.
Citation - WoS: 6
Citation - Scopus: 6
Synthesis and Characterization of Bionanomaterials and Evaluation of Their Antioxidant, Antibacterial, and DNA Cleavage Activities
(Chemistry Select, 2021) Tarhan, Tuba; Dündar, Abdurrahman; Okumuş, Veysi; Çulha, Mustafa; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
In this study, the hexagonal boron nitride (hBN) (1), poly-levodopa (P-L(DP) (2), P-L(DP) coated hBN (hBN@P(L-DP)) (3), and silver nanoparticles (AgNPs) decorated hBN@P(L-DP) (hBN@P(L-DP)-AgNPs) (4) were synthesized and characterized by multiple spectroscopic techniques. Additionally, their biological properties such as antioxidant, antibacterial, and DNA cleavage activities were determined. It is worth noting that, products 3 and 4 were newly synthesized structures. The antioxidant activities of all the nanomaterials 1, 2, 3, and 4 nanomaterials were investigated using some tests such as DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and reducing power ability. Among the synthesized nanomaterials, product 2 exhibited the highest radical scavenging (64.8±1.94 %) and reducing power activity (0.61±0.017) at a concentration of 200 mg L−1. Product 4 was determined to have antibacterial activity against all three Gram-positive and three Gram-negative test bacteria. In addition, all the nanomaterials were tested for cleavage activity using pBR 322 plasmid DNA, as a result of which it was determined that only product 4 showed the ability of cleavage from both chains of DNA.
Citation - WoS: 3
Citation - Scopus: 3
Removal of carbol fuchsin from aqueous solution by using three-dimensional porous, economic, and eco-friendly polymer
(Water Environment Research, 2021) Tarhan, Tuba; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
In this study, a three-dimensional (3D) porous polydimethylsiloxane (PDMS) was prepared using a cheap material with a highly simple and different method. PDMS was firstly applied for the removal of carbol fuchsin (CF) cationic organic dye pollution in this study. Besides, the adsorption capacity of 3D PDMS for removal of the CF was found quite high compared to other materials in already published results. The synthesized PDMS was characterized using several spectroscopic and imaging techniques such as FTIR, Raman, SEM, stereomicroscope, EDX, UV/Vis, and TGA. The optimal conditions were found as 10 mg L−1 initial concentration, 20 mg of adsorbent dose, 2 h contact time, pH 10, and 25°C temperature. The removal % of CF and the maximum adsorption capacity were calculated at approximately 89% and 88.8 mg g−1, respectively. Furthermore, the equilibrium studies showed that the Langmuir isotherm model fitted well with the removal of CF. Moreover, according to the kinetic results, the second-order kinetic model was found suitable (qe,cal 89.3 mg g−1 and qe,exp 88.8 mg g−1 close to each other) for the adsorption of CF. Also, the thermodynamic studies indicated that adsorption occurs spontaneously, and the adsorption process was physical adsorption. Besides, the reusability of the adsorbent was studied. Practitioner points: Water treatment technology should be low cost, economically viable and in the meantime, eco-friendly. The 3D porous PDMS was prepared by using cheap material with a highly simple method and eco-friendly This unique material was firstly applied for the removal of organic dye in water in this study.
Citation - WoS: 17
Citation - Scopus: 16
Newly Synthesized Multifunctional Biopolymer Coated Magnetic Core/Shell Fe3O4@Au Nanoparticles for Evaluation of L-asparaginase Immobilization
(SpringerLink, 2022) Tarhan, Tuba; Dik, Gamze; Ulu, Ahmet; Tural, Bilsen; Tural, Servet; Ateş, Burhan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
The immobilization strategy can promote greater enzyme utilization in applications by improving the overall stability and reusability of the enzyme. In this work, the L-asparaginase (L-ASNase) obtained from Escherichia coli was chosen as a model enzyme and immobilized onto the Fe3O4@Au-carboxymethyl chitosan (CMC) magnetic nanoparticles (MNPs) through adsorption. TEM, SEM, FT-IR, XRD, EDS, and TGA analyses were performed to examine the structure with and without L-ASNase. The yield of immobilized L-ASNase on Fe3O4@Au-CMC was found to be 68%. The biochemical properties such as optimum pH, optimum temperature, reusability, and thermal stability of the Fe3O4@Au-CMC/L-ASNase were comprehensively investigated. For instance, Fe3O4@Au-CMC/L-ASNase reached maximum activity at pH 7.0 and the optimum temperature was found to be 50 °C. The noticeably lower Ea value of the Fe3O4@Au-CMC/L-ASNase revealed the enhanced catalytic activity of this enzyme after immobilization. The Km and Vmax values were 3.27 ± 0.48 mM, and 51.54 ± 0.51 μmol min−1 for Fe3O4@Au-CMC/L-ASNase, respectively, which means good substrate affinity. The Fe3O4@Au-CMC/L-ASNase retained 65% of its initial activity even after 90 min at 60 °C. Moreover, it maintained more than 75% of its original activity after 10 cycles, indicating its excellent reusability. The results obtained suggested that this investigation highlights the use of MNPs as a support for the development of more economical and sustainable immobilized enzyme systems