Tarhan, Tuba

Tarhan, Tuba

Job Title

Doçent

Main Affiliation

Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü

Scholarly Output

34

Articles

12

Citation Count

0

Supervised Theses

1 Scholarly Output Search Results

Now showing 1 - 10 of 30

Synthesis of Drug Loaded Magnetic Nanocomposite and Investigation of Cytotoxic Properties
(2023) Tarhan, Tuba
This aims of study to develop drug delivery systems providing an alternative solution to cancer chemotherapy. Firstly, carboxymethyl dextran which has magnetic properties (MD), was synthesized as stated in the literature.1-3 Its structure was elucidated with various analytical devices. Then, loading studies were carried out on the nanocomposite with topotecan (TP) (in the range of 0.2-40 µM in phosphate buffer at pH 5) as an anticancer drug. The cytotoxic properties of MD, TP, drug loaded MD (MD-TP), and free TP were studied on human prostate cancer cell (Du145) and human healthy prostate cell (PNT1A) line. The synthesized MD and MD-TP nanocomposites were characterized by different analytical devices such as FTIR, XPS, UV-VIS, VSM, TEM, SEM and DLS. According to the FTIR results, the presence of peaks belonging to functional groups in the expected places indicates that MD was successfully synthesized and TP was successfully loaded on nanocomposite. According to TEM image, the MD particles showed a spherical structure, and also, the particle size was measured as 9 ± 1.8 nm. The SEM image of MD indicated that the nanoparticles were aggregated. Furthermore, after the loading TP, the brightly colored particles of TP were observed on some parts of the surface of MD. Moreover, DLS measurements of the nanoparticles were taken before and after loading the drug. Due to high agglomeration of the magnetic nanoparticle, it showed polydispersity. In addition, cytotoxicity effects of drug loaded nanocomposites on Du145 and PNT1A cell lines were investigated and the results were found to be statistically significant. The topotecan loaded MD-TP nanocomposite have shown cell viability to be reduced by up to 20 %. It has been shown that synthesized polymer coated superparamagnetic nanocomposites with these properties can be used in drug targeting studies.
FABRICATION AND EVALUATION OF POLYDOPAMINE (PDA) COATED HEXAGONAL BORON NITRIDE (HBN) MODIFIED POLYDIMETHYLSILOXANE (PDMS) 3D POROUS SCAFFOLD FOR IMPROVE OSSEOINTEGRATION
(2020) Tarhan, Tuba
ABSTRACT: Purpose: Our goal is to enhance poor performance in supporting cell growth and proliferation in osseointegration. Herein, we fabricated a new scaffold material which was Polydimethylsiloxane (PDMS) based 3D structure, biocompatibility, cytocompatibility, and porous. Materials and Methods: In this study, the scaffold was fabricated as polydopamine (PDA) coated hexagonal boron nitride (hBN) embedded in PDMS. First, 3D porous structure PDMS was prepared using two agents of PDMS with the salt scaffold method. In the second step, the scaffold was prepared with PDA coated hBN was embedded in PDMS. Nowadays, the application of PDA as a superior surface modifier in multifunctional biomaterials is drawing tremendous interests in bone tissue scaffolds to promote the osseointegration for bone regeneration. The hBN was used to increase the durability of PDMS. Because, it possesses preeminent physical and chemical properties such as high chemical stability, temperature-resistant, high thermal conductivity, low density, good mechanical strength, anti oxidation ability, and biocompatibility. To perfectly the cells adhere to the surface, the hBN was coated with PDA. In the third step, the prepared scaffold was mineralized for the proliferation of cells. Results: The prepared PDMS-based 3D scaffolds were characterized by Raman and Fourier transform infrared (FT-IR) spectroscopies. The structure of morphology was studied using the scanning electron microscope (SEM). The compression and tensile stress as mechanical properties of the scaffolds were evaluated for the PDMS, PDMS- hBN-PDA, and its mineralized form PDMS- hBN-PDA-Min. The results of the tensile strength scaffolds of PDMS-hBN-PDA-Dry and PDMS-hBN-PDA-Wet were calculated 588.4 kPa and 614.7 kPa, respectively. However, the tensile strength of 3D PDMS-Dry and PDMS-Wet were calculated 285.3 kPa and 423.2 kPa, respectively. The tensile strength value of PDMS hBN-PDA-Dry was 53.6 % higher than that of 3D PDMS-Dry scaffold and also, elongation at break value of PDMS-hBN-PDA-Dry was 16.7 % higher than that of pristine PDMS-Dry scaffold. The contact angles were measured for the PDMS, PDMS- hBN-PDA, and PDMS- hBN-PDA-Min. scaffolds with deionized water droplets. The average contact angles (CAs) other mean surface wettability is a very important parameter for cell adhesion performance. The CAs were measured for the PDMS, PDMS hBN-PDA, and its mineralized form PDMS- hBN-PDA-Min scaffolds with deionized water droplets. The CAs of the PDMS was measured 87.6° ± 1.15 and after hBN-PDA was dispersed in PDMS its decreased around 72.1° ± 1.69. This lower CA was related to PDA containing the hydrophilic groups such as catechol, amide, and imide groups. Furthermore, after the mineralization process, the CA was decrease than before and it was found around 71.3° ± 1.20. This is because the catechol group of PDA was capturing some cations such as Ca2+ and K+ . The literature shows that the CA of scaffolds is at around 65°–70° the appropriate values for the cell adhesion and proliferation. The thermal stability and degradation mechanism of scaffolds were investigated using Thermogravimetric analysis (TGA). According to the results, while embedding hBN in PDMS showed higher resistance to temperature than pristine PDMS, the mineralization process showed the same curve as PDMS. Conclusion: In conclusion, PDMS is routinely used as a biomedical implant material and for fundamental cellular studies and has been confirmed as a biocompatible material. Moreover, the hBN used in the fabrication of scaffold is a novel nanostructure it has excellent properties such as high mechanical strengths, large surface area, and outstanding biocompatibilities. The application of PDA as a superior surface modifier in multifunctional biomaterials will be drawing tremendous interests in bone tissue scaffolds to promote the osteointegration for bone regeneration. Considering all these features and the results of characterization studies, we foresee that the prepared PDMS-hBN-PDA can be used as a perfect scaffold material in the future for cell adhesion and proliferation in osseointegration. Besides, a new 3D scaffold model based on PDMS was designed for bone tissue. Key Words: Bone repairing materials, Polydimethylsiloxane, Hexagonal boron nitride nanoparticles, dopamine, Scaffold.
Kanser Araştırmalarına Yönelik Manyetik O-Karboksimetil Kitosan Nanopartiküllerin Sentezlenmesi, Karakterizasyonu, İrinotekan yüklenmesi ve Glioblastoma Multiforme (Beyin Tümörü) Hücre Hatları Üzerine Sitotoksisite Değerlendirilmesi
(2020) Tarhan, Tuba
Kanser basit bir ifadeyle kontrolsüz hücre çoğalması olarak tanımlanabilir. Kelime anlamı olarak kanser, bir organ veya dokudaki hücrelerin düzensiz olarak bölünüp çoğalmasıyla beliren kötü urlara denir. Kanser 100'den fazla hastalık grubunu içerir. Çok çeşitli kanser tipleri olmasına rağmen, hepsi anormal hücrelerin kontrol dışı çoğalması ile başlar. Tedavi edilmez ise ciddi rahatsızlıklara, hatta ölüme dahi neden olabilir (Türkiye Halk Sağlığı Kurumu, Kanser Daire Başkanlığı). Dünya istatistiklerinde her yıl kansere yakalanan kişi sayısının 12,7 milyon olduğu tahmin edilmekte ve bu sayının 2030 yılında 21 milyona yükselmesi beklenmektedir. Farklı ülkelerde farklı kanser türlerinin oranları yüksek olsa da dünyada ölüm nedenlerinin başında kanser gelmektedir (World Cancer Research Fund, 2013). Çok yaygın olarak görülen ve ölüm oranları yüksek olan bu hastalıkta genelde uygulanan tedavi yöntemleri kemoterapi, radyoterapi ve ameliyat ile sınırlıdır. Bu yöntemler yan etkisi çok olan invazif yöntemlerdir. Kanser tedavisi için en çok kullanılan yöntemlerden biri kemoterapi yöntemidir. Günümüzde kanser kemoterapisinin en önemli sorunlarından biri, kullanılan anti-kanser ilaçlarının kanserli hücreyi ayırt edici özelliğe sahip olmamaları ve yan etki olarak sağlıklı hücrelerin üzerinde de toksik etki göstermeleridir. Ayrıca tedavi edici doz konsantrasyonunu sağlamak için vücuda yüksek dozda ilaç verilmesi bununla birlikte ciddi yan etkilerin ve sistemik toksisitenin ortaya çıkmasıdır. Bu tür sorunların önüne geçebilmek için, nanoteknolojideki hızlı ilerleme, diğer alanlar ile birlikte sağlık alanında da önemli gelişmeleri beraberinde getirmiştir. Nanotıp (ya da nanoilaç) bir hastalığın tedavisi ya da hasarlı bir dokunun onarılması için moleküler düzeyde yüksek özgünlük gösteren bir medikal uygulama olarak tanımlanmıştır. Son zamanlarda ilaç endüstrisinde, teşhis ve tedavilerde, görüntüleme tekniklerinde, vücutta kontrollü ilaç salınımında ve ilacı hedef dokuya ulaştırmada, doku mühendisliğinde ve daha pek çok alanda nanoteknolojiden faydalanılmaktadır. Birçok farklı nanosistemden özellikle çok fonksiyonlu manyetik nanoparçacıklar (MNPs) bu alanda ilgi odağı olmaktadır. MNPs’ın manyetik alan altında yönlendirilebilme özelliği onları diğer nanoparçacıklara göre daha avantajlı kılmaktadır. Yeni nesil tedavi yöntemlerinde sağlıklı hücrelere zarar vermeden ilacı hedef bölgeye ulaştırma ve etkin bir tedavi için özellikle MNPs’ın kullanımları ve avantajları araştırılmaktadır. Bu projenin özgün değeri, kanser kamoterapisinden kaynaklı problemlere çözüm bulmak amacı ile son yıllarda kontrollü ilaç salım sistemleri üzerine yapılan çalışmalara alternatif bir çözüm geliştirmektir. Nano boyuttaki kontrollü ilaç taşıma sistemleri tümör hedeflenmesine olanak sağlamaktır. Nano taşıyıcılar arasından, biyouyumlu polimer kaplı paramanyetik nanoparçacıklar dışardan uygulanan manyetik alan ile istenilen bölgeye hedeflenebilme özelliklerine sahiptirler. Bu sayede ilaç hedeflenen bölgeye güvenli bir şekilde taşınabilmektedir. Bu parçacıklar gerekli modifikasyonlarla ilaç yüklenmeye uygun ve manyetik alan ile istenilen bölgeye ulaştırılabilen parçacıklar haline getirilebilmektedirler. Ayrıca, etken maddenin dozunun azaltılabilmesine ve etken maddenin sadece hedef bölgeye dağılımıyla sınırlandırılmasına olanak sağlayabilmektedir. Bu yöntem ile geleneksel kamoterapinin aksine sadece tümörlü hücrelere müdahale edilebilir ve ilaçların sağlıklı hücrelere etkisi azaltılabilir. Bu proje kapsamında en yaygın kullanılan anti-kanser ilaçlardan Kamptotesin analoğları ile çalışılacaktır. Kamptotesin topoizomeraz I inhibitörü olarak görev yapmaktadır. Bu enzim hücre bölünmesi esnasında DNA replikasyonundan sorumlu enzimdir. Kamptotesin analogları yapısında α-hidroksi δ-lakton halkası bulundurmaktadır. Ayrıca fizyolojik pH’ta ve alkali ortamda lakton halkası hidroliz olup karboksilat forma dönüşmektedir, bu haliyle stabil formdan uzaklaşıp vücut için toksik bir hal almaktadır. Dolaysıyla çalışılması zor olan ilaçlar arasında olup bununla ilgili yapılan çalışmaların çoğu patentlidir. Projemizin amacı, anti-kanser ilaç olarak önemli bir yere sahip olan Kamptotesin analoğlarının lakton formunda kararlı kalmasını sağlayan koşulları tespit etmek ve daha önce bu anti-kanser ilaçlar ile çalışılmamış manyetik özellik gösteren biyouyumlu ve biyobozunur polimerik madde sentezleyip, bu polimer moleküllerine ilacın yüklenmesi, taşınması ve salımını çalışmaktır.
Chiral Resin - Coated Magnetic Nanoparticles for Resolution of Chiral Mandelic Acid
(2012) Tarhan, Tuba
Bu çalışmada, bir meyve asidi olup acı bademden elde edilen ve farmakolojik öneme sahip olan (R)- ve (S)- mandelik asidin rezolüsyonu sağlandı. (R)-mandelik asit uzun yıllar boyunca tıpta, anti bakteriyel olarak özellikle idrar yolu enfeksiyonları tedavisinde, cilt hastalıkları tedavisinde, cilt bakım ürünlerinde, yetişkinlerde akne sorunlarını gidermede, rosacea (gül hastalığı ) hastalığı tedavisinde, iltihaplanma ve kızarıklıkları gidermede kullanılmaktadır. Ayrıca yarı sentetik sefalosprinler ve penisilinlerin üretiminde anahtar bir ara madde özelliği göstermektedir. Üstelik anti-tümör ve anti-obezite ajanlarının sentezi için kiral sinton ve kiral bir ayırma ajanı olarak kullanılmaktadır. Ayrıca antibiyotik olan ilacın aktivitesini geliştirmede yaygın olarak kullanılır. (S)- mandelik asit ise daha çok hayvan sağlığında ve hayvan bakım ilaçlarında kullanılmaktadır. Farmakolojik olarak farklı etkilere sahip bu iki enantiyomerin rezolüsyonunu sağlamak tıpta ve ilaç sanayisinde önem arz etmektedir. Bu çalışmada manyetik özellik gösteren nano parçacıklar(Fe3O4) silika ile kaplandı. Daha sonra kiral ayırmada etkinliği olan bazı fonksiyonel gruplar silika üzerine kovalent bağlanarak amaca uygun bir reçine sentezlendi. Sonrasında rasemik mandelik asit karışımı hazırlanıp değişik pH' lardaki (pH 2.2; 3.5;4.0; 4.4?) fosfat çözeltisinde çözüldü. Elde edilen rasemik karışım daha önce sentezlenmiş olan manyetik nanoparçacıklar içeren reçineyle, mekanik bir karıştırıcı yardımı ile etkileştirildi. Değişik zaman aralıklarında (1 dk, 5 dk, 10 dk, 20 dk ?) alınan eluatlar eter (dietileter) ile ekstrakte edilip eter fazı evapore edildi ve geriye kalan beyaz katı n-heksan-isopropanol karışımına alınıp HPLC de analiz edilerek enantiyomerik fazlalık(e,f) hesaplandı. Manyetik nanoparçacıklar kullanılarak yapılan enantiyomerik ayırmalarla ilgili çalışmalar literatürde çok az rastlanmaktadır. Bu tür çalışmaların literatürde 2010 ve sonraki yıllarda yayınlanmaya başladığını görüyoruz. Amacımız henüz yeni kullanılacak olan bu yöntem ile gelecekte yapılacak olan bu tür çalışmalara yeni bir umut ışığı olmak ve katkıda bulunmaktır.
Maltose Functionalized Magnetic Core/Shell Fe₃o_{4< Nanoparticles for an Efficient L-Asparaginase Immobilization}
(Elsevier, 2020) Tarhan, Tuba; Ulu, Ahmet; Saricam, Melike; Culha, Mustafa; Ates, Burhan
In this study, maltose-functionalized magnetic core/shell nanoparticles (Fe3O4@Au NPs) as a promising carrier matrix for a simple and effective immobilization of L-asparaginase (L-ASNase) were prepared and characterized using imaging techniques including atomic force microscopy (AFM) and transmission electron microscopy (TEM), and vibrating sample magnetometry (VSM). The results indicate that the NPs are monodispersed with an average diameter of 10 nm and magnetization of 9.0 emu g(-1). Under the optimal conditions, 77.2 +/- 2.3% of the total L-ASNase was immobilized. It was found that the acid-base tolerance and thermal stability of immobilized L-ASNase were significantly improved in comparison to the free form of the enzyme in solution. For instance, while only 10% of the immobilized enzyme was lost its activity, the free form was lost its activity more than 50% after 3 h incubation at 55 degrees C. After 13 times recycling, the immobilized L-ASNase retained about 50% of its initial activity. Moreover, the free and immobilized L-ASNase maintained their initial activities about 25 and 64% after 28 days storage at 25 degrees C, respectively. Km value of immobilized L-ASNase decreased to 1.59 from 2.95 mM as an indication of increased enzyme affinity for the substrate. The results of this study suggest that the maltose-coated magnetic nanoparticles are excellent nanovehicles to carry enzymes for a range of industrial applications. (C) 2019 Elsevier B.V. All rights reserved.
Synthesis and Characterization Magnetic Nanocomposites as Chemotherapy Agents
(2018) Tarhan, Tuba
One of the most imp cancer cells as a side effect1,2. Studies on controlled drug release systems have increased with the aim of finding solutions to these problems3 . Controlled drug delivery systems at the nanoscale enable tumor targeting. Among the nanocarriers, biopolymer coated superparamagnetic nanoparticles have the ability to target at the desired region with an externally applied magnetic field. In this way, the drug can be safely delivery to the targeted area.3 The purpose of this study is, suitable for carrying the mentioned therapeutic agents, to have suitable magnetic, chemical and physical properties for use in biomedical applications, magnetic targeting, synthesis and characterization of branched polymeric materials with carboxyl groups4,5,6 . Biocompatible and biodegradable polymers is used as Chitosan. In addition, different derivatives of these polymers were synthesized and characterized by FTIR, TEM, SEM, EDX, DLS, XPS and VSM. As a results obtained that biocompatible and biodegradable magnetic nanocomposites were of the desired size and superparamagnetism, It has been shown that synthesized polymer coated superparamagnetic nanocomposites with these properties can be used in drug targeting studies and it is hoped that this type of work will contribute to future studies.
Removal of carbol fuchsin from aqueous solution by using three-dimensional porous, economic, and eco-friendly polymer
(Water Environment Research, 2021) Tarhan, Tuba
In this study, a three-dimensional (3D) porous polydimethylsiloxane (PDMS) was prepared using a cheap material with a highly simple and different method. PDMS was firstly applied for the removal of carbol fuchsin (CF) cationic organic dye pollution in this study. Besides, the adsorption capacity of 3D PDMS for removal of the CF was found quite high compared to other materials in already published results. The synthesized PDMS was characterized using several spectroscopic and imaging techniques such as FTIR, Raman, SEM, stereomicroscope, EDX, UV/Vis, and TGA. The optimal conditions were found as 10 mg L−1 initial concentration, 20 mg of adsorbent dose, 2 h contact time, pH 10, and 25°C temperature. The removal % of CF and the maximum adsorption capacity were calculated at approximately 89% and 88.8 mg g−1, respectively. Furthermore, the equilibrium studies showed that the Langmuir isotherm model fitted well with the removal of CF. Moreover, according to the kinetic results, the second-order kinetic model was found suitable (qe,cal 89.3 mg g−1 and qe,exp 88.8 mg g−1 close to each other) for the adsorption of CF. Also, the thermodynamic studies indicated that adsorption occurs spontaneously, and the adsorption process was physical adsorption. Besides, the reusability of the adsorbent was studied. Practitioner points: Water treatment technology should be low cost, economically viable and in the meantime, eco-friendly. The 3D porous PDMS was prepared by using cheap material with a highly simple method and eco-friendly This unique material was firstly applied for the removal of organic dye in water in this study.
Synthesis and Characterization of Hexagonal Boron Nitride (hBN) for an Effective Removal of Organic Dyes: Kinetic and Equilibrium Studies
(2020) Tarhan, Tuba
In this study, hBN nanostructure was synthesized from boric acid to remove organic dyes from aqueous solution. The characteristic peaks of hBN are obtained using Raman and Fourier Transform Infrared (FT-IR) spectroscopies. However, the morphology and particle size of hBN are determined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). During the studies, various key factors such as pH of the aqueous dye solution, initial dye concentration, adsorbent dose and contact time are investigated. Additionally, equilibrium isotherm and kinetic models are studied for the removal of anionic and cationic organic dye Metanyl Yellow (MY) and Victoria Blue B (VBB), respectively. Under optimal conditions, it was found that 42.6% Methanyl Yellow (MY) and 90% Victoria Blue B (VBB) were removed from aqueous solution using the hBN nanostructure.
A Detailed Review on the Green Synthesis of Selenium Nanoparticles Using Plant Extracts and Their Anticancer Applications
(Wiley-v C H verlag Gmbh, 2024) Tarhan, Tuba
Nanobiotechnology is a field of science with tremendous promising applications in cancer chemotherapy, targeted drug delivery, molecular diagnosis, and molecular imaging. Selenium, one of the critical trace elements, participates in the structure of selenocysteine protein, which plays an important role in maintaining redox balance due to its oxidoreductase activity. Elemental selenium (Se0) has significantly lower toxicity values than its oxyanions, selenite, and selenate forms. For this reason, it is important to convert elemental selenium into atomic form in biological studies. In particular, their role in regulating the immune system makes selenium nanoparticles (SeNPs) superior to others. SeNPs have been discussed effectively in the literature in anticancer activity applications. SeNPs green synthesis is focused on a more economical, biocompatible, and environmentally friendly application by using, reducing, and stabilizing agents in plants. The aim of this review is to highlight the anticancer activity applications of SeNPs synthesized with plant extracts in the last 6 years (i.e., between 2019 and 2024). In addition, it aims to shed light on the green synthesis mechanism of NPs by using plant extracts and to highlight the factors affecting green synthesis and its advantages and disadvantages.
Synthesis and Characterization of Branched Magnetic Dextran with Carboxyl Group for Drug Delivery System
(2018) Tarhan, Tuba
Cancer is called bad urine, which is seen when an organ or tissue cells divide and multiply irregularly. It is among the major causes of death and rapidly increasing all over the world. Latest statistical information based on the number of cancer patients in the world is pointed out that more than 15 million in 2020 (World Cancer Research Fund, 2013). Such measure against a disease that threatens the world health and initiation of treatment is sometimes insufficient. Hence scientists were forced to develop new treatments. It has endemic and high mortality rates which is applied treatment methods usually chemotherapy, radiotherapy and surgery in this disease. Chemotherapy is used one of the most methods in cancer treatment. One of the most important problems of cancer chemotherapy nowadays is used that the anti-cancer drugs do not have the ability to recognize cancer cells and show toxic effects on healthy cells 1,2,3. In addition, administration of high doses of the drug is given to the body to provide a therapeutic dose concentration, this situation is causes severe side effects and systemic toxicity, and if not controlled it can result in death. Therefore, delivering the drug to target cell within the body and developing of drug delivery system providing controlled release are of great importance. In recent years, the development of the systems in which drug is delivered magnetically to the target is drawing considerable attention since it is a current issue. It is possible to eliminate the most of the problems caused by high doses of chemotherapy by using the magnetic drug delivery systems. However, in some studies in the literature, it is seen that toxicity increase in nanoparticle applications above a certain concentration even in the magnetic drug delivery systems. Therefore, it is important to design delivery systems with high drug loading capacity. The design of magnetic drug delivery systems is require consideration of several factors including magnetic properties, particle size and drug loading capacity. It is necessary to increase the number of reactive groups on the surface of nanoparticles in order to increase drug loading capacity. The polymeric nanoparticles comparison with structure of kollaidal carrier they brought together more stable in biological fluids, the polymeric structures to be more tightly controlled to provide sustained drug release and some of the advantages of polymer. In recent years that can be especially biodegradable polymeric nanoparticles implemented to in drug delivery targeted organ or tissue and can be used as DNA carriers in gene therapy take of attention. The nanoparticles can be used to purpose of drug delivery that owner physico-chemical properties such as hyperthermia property, magnetic resonance imaging (MRI) feature can view the body, biocompatibility, particle size, toxicity, surface charge, drug adsorption capacity, surface hydrophobicity, loading ratio, release kinetics and stability, It can be evaluated 4,5. The original value of this study can develop an alternative solution to studies on controlled drug release systems which is to solve problems arising from cancer chemotherapy. Polymer coated nanocarrier have the ability to be targeted to the desired site by the magnetic field applied from outside. In contrast to conventional chemotherapy, this method can only interfere with tumor cells and reduce the effect of the drug on healthy cells 6,7. For this purpose, dextran was used as biocompatible and biodegradable polymer to have suitable magnetic, chemical and physical properties for use in biomedical applications and magnetic targeting. In addition, branched magnetic dextran was synthesized by using magnetic O-carboxymethyl dextran and NαNα-Bis (carboxymethyl) -L-lysine hydrate (NTA) in order to increase the number of reactive carboxyl groups on the surface of magnetic dextran8,9,10,11,12 and synthesized branched magnetic dextran was characterized by different analytical techniques such as Transmission Electron Microscopy (TEM), scanning electron microscope (SEM), Dynamic Light Scattering Spectrometer (DLS), Vibrating Sample Magnetometer (VSM), Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Photoelectron Spectroscopy (XPS). EDX, XPS, SEM, TEM, FTIR, DLS and VSM analysis techniques were used for elemental analysis, size and surface morphology, structural analysis and magnetic properties.