Tarhan, TubaDik, GamzeUlu, AhmetTural, BilsenTural, ServetAteş, Burhan2023-01-172023-01-172022https://doi.org/10.1007/s11244-022-01742-yhttps://www.scopus.com/record/display.uri?eid=2-s2.0-85141947662&origin=SingleRecordEmailAlert&dgcid=raven_sc_affil_en_us_email&txGid=0f2337774b62f70cf19e167fcd3dffabhttps://hdl.handle.net/20.500.12514/3331The immobilization strategy can promote greater enzyme utilization in applications by improving the overall stability and reusability of the enzyme. In this work, the L-asparaginase (L-ASNase) obtained from Escherichia coli was chosen as a model enzyme and immobilized onto the Fe3O4@Au-carboxymethyl chitosan (CMC) magnetic nanoparticles (MNPs) through adsorption. TEM, SEM, FT-IR, XRD, EDS, and TGA analyses were performed to examine the structure with and without L-ASNase. The yield of immobilized L-ASNase on Fe3O4@Au-CMC was found to be 68%. The biochemical properties such as optimum pH, optimum temperature, reusability, and thermal stability of the Fe3O4@Au-CMC/L-ASNase were comprehensively investigated. For instance, Fe3O4@Au-CMC/L-ASNase reached maximum activity at pH 7.0 and the optimum temperature was found to be 50 °C. The noticeably lower Ea value of the Fe3O4@Au-CMC/L-ASNase revealed the enhanced catalytic activity of this enzyme after immobilization. The Km and Vmax values were 3.27 ± 0.48 mM, and 51.54 ± 0.51 μmol min−1 for Fe3O4@Au-CMC/L-ASNase, respectively, which means good substrate affinity. The Fe3O4@Au-CMC/L-ASNase retained 65% of its initial activity even after 90 min at 60 °C. Moreover, it maintained more than 75% of its original activity after 10 cycles, indicating its excellent reusability. The results obtained suggested that this investigation highlights the use of MNPs as a support for the development of more economical and sustainable immobilized enzyme systemsen10.1007/s11244-022-01742-yinfo:eu-repo/semantics/closedAccessCarboxymethyl chitosan; Enhanced stability; Enzyme carrier; Fe3O4@Au; L-asparaginase immobilizationArticle115Q2WOS:0008857173000012-s2.0-85141947662