Unsal, VelidKölükçü, EnginAtılgan, DoğanUluocak, NihatDeresoy, Faik AlevKatar, Muzaffer2021-07-282021-07-282021https://www.scopus.com/record/display.uri?eid=2-s2.0-85107237811&doi=10.1111%2fand.14128&origin=inward&txGid=308a59da7a8e09853bbf4486fce8b125https://hdl.handle.net/20.500.12514/2708https://pubmed.ncbi.nlm.nih.gov/34091938/This experimental study aims to evaluate the efficacy of milrinone against ischaemia-reperfusion injury due to testicular torsion/detorsion. Group 1 was defined as the control group. Testicular torsion/detorsion model was performed in Group 2. Group 3 had similar procedures to the rats in Group 2. In addition, 0.5 mg/kg of milrinone was administered intraperitoneally immediately after testicular torsion in Group 3. Histopathological examinations indicated a dramatic improvement in terms of inflammation, haemorrhage, oedema, congestion, Cosentino and Johnson scores in Group 3 compared to Group 2 (p =.037, p =.045, p =.018, p =.040, p =.033 and p =.03 respectively). Blood biochemical analyses, superoxide dismutase (SOD), glutathione peroxidase (GSH-px) activity and total antioxidant status (TAS) levels increased significantly in Group 3 compared to Group 2 (p =.001, p =.024 and p <.001). Malondialdehyde (MDA), protein carbonyl (PC), interleukin 1beta (IL-1beta), tumour necrosis factor-alpha (TNF-alpha) and total oxidant status (TOS) levels decreased in Group 3 compared to Group 2 (p =.001, p =.018, p <.001, p =.036 and p =.002 respectively). Tissue biochemical analyses determined an increase in SOD and GSH-px activity in Group 3 compared to Group 2, while PC and MDA levels were reduced (p =.001, p <.001, p =.038 and p <.001 respectively). Milrinone attenuates ischaemia-reperfusion injury that causes highly harmful effects due to testicular torsion/detorsion.eninfo:eu-repo/semantics/closedAccessischaemia-reperfusion injury, milrinone, testicular torsion, treatmentArticleQ3WOS:0006580476000012-s2.0-8510723781134091938