Acet, ÖmürBaran, TalatErdönmez, DemetAksoy, Neşe HayatAlacabey, İhsanMenteş, AyferOdabaşı, Mehmet14.07.20192019-07-1614.07.20192019-07-1620180021-96731873-3778https://doi.org/10.1016/j.chroma.2018.03.022https://hdl.handle.net/20.500.12514/1257We synthesized Ni2+-attached O-Carboxymethyl chitosan Schiff base complexes embedded composite cryogels (Ni2+-O-CMCS-CCs) by means of polymerization of gel-forming precursors at subzero temperatures. Prepared affinity cryogel showed excellent adsorption performance for lysozyme selected as model protein to test adsorption parameters, demonstrating an adsorption capacity of 244.6 mg/g (15.3 mg/g for Ni2+ minus O-CMCS-CCs), with fast adsorption equilibrium within 30 min and good reversibility. The performance of Ni2+-O-CMCS-CCs for lysozyme was also evaluated by SDS-PAGE, and a purification efficiency of 86.9% with 89.5% purification yield was determined. The swelling test, FT-IR, and SEM analysis were carried out for the characterization of Ni2+-O-CMCS-CCs. At the end of 35 adsorption-desorption cycles, there was no significant change in the adsorption capacity. (C) 2018 Elsevier B.V. All rights reserved.en10.1016/j.chroma.2018.03.022info:eu-repo/semantics/closedAccessCryogelIMACCarboxymethyl chitosanLysozyme adsorptionArticle15502127Q1Q1WOS:0004308974000032-s2.0-8504453143529609862