Kolukcu, VildanUnsal, VelidKatar, MuzafferBalta, Mehtap GuerlerTapar, HakanKaraman, TugbaKuyucu, Yunus Emre2025-02-152025-02-1520240250-46851303-829Xhttps://doi.org/10.1515/tjb-2024-0111https://hdl.handle.net/20.500.12514/6071KOLUKCU, VILDAN/0000-0002-3914-3899; Yalcin, Kenan/0000-0003-3560-5862; UNSAL, VELID/0000-0003-1415-0563; karaman, tugba/0000-0002-0724-3326; Karaman, Serkan/0000-0003-0534-629X; Katar, Muzaffer/0000-0002-6296-2390; Gevrek, Fikret/0000-0002-3722-2542Objectives This study aims to evaluate the protective efficacy of remifentanil against testicular ischemia-reperfusion injury. Methods The study included 24 male rats. The rats were randomized into three groups: Group 1 was the control group. Group 2 was subjected to a testicular torsion/detorsion model. Group 3 underwent similar procedures and additionally received remifentanil (0.6 mu g/kg/min) intravenously for the first 20 min of reperfusion. Blood samples were taken for biochemical analyses, and orchiectomy was performed for histopathologic examination. Results Biochemical analysis of blood samples showed a significant increase in antioxidant enzyme activity, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in Group 3 compared to Group 2 (p:0.004 and p:0.002, respectively). There was a dramatic decrease in the levels of proinflammatory cytokines, including interleukin-1 beta (IL-1 Beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) in Group 3 compared to Group 2 (p:0.001, p:0.046, and p:0.004, respectively). Similarly, malondialdehyde (MDA) levels decreased in Group 3 compared to Group 2 (p:0.004). Histopathologic examination of Group 3 rats showed positive changes in inflammation, hemorrhage, edema, and congestion levels compared to Group 2 (p<0.001). Similarly, there was a positive effect on the Johnsen and Cosentino score in Group 3 compared to Group 2 (p:0.001 and p<0.001, respectively). Conclusions In our study, it has been documented that remifentanil protects against testicular ischemia-reperfusion injury.en10.1515/tjb-2024-0111info:eu-repo/semantics/openAccessRemifentanilTestisIschemia-Reperfusion InjuryArticle496801809Q4Q4WOS:0013146066000012-s2.0-852048028670