Tıbbi Biyokimya Anabilim Dalı Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/4222

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Can Serum Biomarker Values from Second-Trimester Aneuploidy Screening Predict the development of Retinopathy of Prematurity in Premature Infants?
(Kare Publishing, 2024) Sabanci, Senol; Kucuk, Mehmet Fatih; Savas, Hasan Basri; Suren, Elcin; Erol, Muhammet Kazim; Yavuz, And; Sipahioglu, Haydar
Objectives: This study aimed to evaluate serum biomarker values measured during second-trimester aneuploidy screening in terms of their predictive ability for the development of retinopathy of prematurity (ROP) in premature infants. Methods: This retrospective cohort study evaluated the data of 1985 idiopathic premature infants who underwent ROP screening from 2016 to 2022. The infants were divided into two groups according to the presence of ROP, and those with ROP were further evaluated in two subgroups based on the presence of proliferation. Comparisons were made concerning the serum multiple of the median values of unconjugated estriol (uE3), human chorionic gonadotropin (hCG), and alpha-fetoprotein (AFP) among aneuploidy screening biomarkers. Results: While 1628 premature infants were in the non-ROP group, 357 were in the ROP group. Of the infants with ROP, 72 were in the proliferative ROP group and 285 in the non-proliferative ROP group. There was no significant difference in the multiple of the median values of the evaluated serum biomarkers (uE3, hCG, and AFP) between the ROP and non-ROP groups or between the proliferative ROP, non-proliferative ROP, and non-ROP groups. Conclusion: The multiple of the median values of second-trimester aneuploidy screening serum biomarkers were not able to predict the development of ROP in premature infants. This result may have been caused by the fact that the blood tests were taken only once and in the same weeks.
Citation - WoS: 5
Citation - Scopus: 5
Comparison of Growth Factor Levels in Injectable Platelet-Rich Fibrin Obtained From Healthy Individuals and Patients With Chronic Periodontitis: a Pilot Study
(BMC, 2024) Savas, Hasan Basri; Karci, Bilge
Background This study aimed to assess and compare the concentrations of growth factors, white blood cells (WBCs), and platelets in injectable platelet-rich fibrin (i-PRF) derived from people with healthy periodontal conditions and those with chronic periodontitis.Methods Venous blood samples were obtained from 30 patients diagnosed with chronic periodontitis (test group) and 30 participants with healthy periodontal conditions (control group). The i-PRF was then acquired from centrifuged blood. The growth factors (VEGF, IGF-1, TGF-beta 1, PDGF-BB and EGF) released from the i-PRF samples were compared between groups with ELISA testing. The amounts of WBCs and platelets were also compared.Results No significant differences in the concentrations of growth factors were found between the groups (the mean values for the control and test groups were, respectively: IGF: 38.82, 42.46; PDGF: 414.25, 466.28; VEGF: 375.69, 412.18; TGF-beta 1: 21.50, 26.21; EGF: 138.62, 154.82). The test group exhibited a significantly higher WBC count than the control group (8.80 vs. 6.60, respectively). However, the platelet count did not show a statistically significant difference between the groups (control group 242.0 vs. test group 262.50). No significant correlation was observed between WBC count and growth factor level in either group.Conclusions The growth factor levels in i-PRFs did not exhibit significant difference between the two groups. This suggests that the levels of these growth factors may be unaffected by the periodontal disease.
Is There a Relationship Between First-Trimester Aneuploidy Screening Serum Biomarker Values and Nuchal Translucency Measurements and the Development of Retinopathy of Prematurity (ROP) in Premature Infants?
(Nature, 2023) Erol, Muhammet; Sabanci, Senol; Kucuk, Mehmet Fatih; Savas, Hasan Basri; Yaprak, Lutfiye; Suren, Elcin
Abstract Purpose The purpose of this study is the evaluation of serum biomarker and nuchal translucency (NT) values measured during first-trimester aneuploidy screening in terms of the development of retinopathy of prematurity (ROP) in premature infants and investigation of whether the development of ROP is associated with these parameters. Methods In this retrospective cohort study, 3,750 premature infants who underwent ROP screening from 2016 to 2021 were identified from the hospital medical record system. Among 2,130 premature babies screened for firsttrimester aneuploidy, 166 babies whose mothers had single pregnancies were screened by the same method and showed the same clinical course in both eyes were included in the study. The infants were divided into two groups according to the presence of ROP, and those with ROP were further evaluated in two groups according to the presence of proliferation. The groups were compared in terms of the serum values of human chorionic gonadotropin and pregnancy-associated plasma protein A, among aneuploidy screening biomarkers, and NT measurements. Results There was no significant difference in the evaluated serum biomarker values and NT measurements between the ROP and non-ROP groups or between the proliferative ROP, non-proliferative ROP, and non-ROP groups. Conclusion Our results showed that first-trimester aneuploidy screening serum biomarker values and NT measurements were not associated with the development of ROP in premature infants.
The Oxidative and Anti-Angiogenic Effects of Acrylamide in Chorioallantoic Membrane Model
(Akdeniz Üniversitesi, 2023) Sozen, Mehmet Enes; Karahan, Oguz; Akkaya, Özgür; Savas, Hasan Basri
Monosodium glutamate (MSG) is a flavor-enhancing food additive. MSG exposure is rising day by day because of the high commercial food consumption. MSG exposure causes damage to various tissues and organs. The aim of this study is to investigate the effects of MSG on angiogenesis and oxidant-antioxidant balance. Three different concentrations of MSG (10-4 M, 10-5 M, and 10-6 M), control, and the bevacizumab (10-6 M) were prepared and placed on the chorioallantoic membrane (CAM) of the embryos. Albumen was taken from the embryos before and after the experiment. Angiogenesis was investigated through the window that was opened on the eggshell. Angiogenesis was found to be normal in the control and 10-6 M MSG group (average score: 0.3). Anti-angiogenic effects were moderate in the 10-5 M MSG group (average score: 0.5) and in the 10-4 M MSG group (average score: 0.7), and strong in the bevacizumab group (average score: 1.1). According to our results, MSG shows anti-angiogenic properties in higher doses. MSG increased oxidative stress. According to the results of our research, it is seen that MSG inhibits angiogenesis in a dose-dependent manner in the CAM model and may cause an increase in oxidative damage by disrupting the oxidant-antioxidant balance. Since no previous study has been found in the literature regarding the effects of MSG on angiogenesis and oxidant-antioxidant balance in the CAM model, we think our results will fill an important gap in the literature.
Citation - WoS: 6
Citation - Scopus: 5
Thiol–disulfide balance and trace element levels in patients with seasonal allergic rhinitis
(African Health Sciences, 2022) Savas, Hasan Basri; Gunizi, Huseyin
Abstract Background: The prevalence of allergic diseases is gradually increasing worldwide. The most common such allergic disease is allergic rhinitis (AR). Objective: The present study investigated the possible relationship between seasonal AR and the thiol-disulfide balance and zinc and copper levels in adult individuals. Study Design and Methods: 130 male and female adults were included in the study. The participants’ serum thiol-disulfide balance and zinc and copper levels were measured spectrophotometrically using commercial kits. Statistical significance was accepted as p < 0.05 between the groups. Results: The serum copper (p = 0.001), native thiol (p = 0.006), reduced thiol (p < 0.001), and thiol oxidation reduction ratio (p < 0.001) levels were significantly lower in the seasonal AR group than in the control group. Conclusion: In AR patients, the low level of copper, which is an important trace element, the deterioration of the thiol-disulfide balance, which represents a unique indicator of the oxidant-antioxidant balance, the increased disulfide level caused by oxidative stress, and the decreased native thiol level can all serve as important biochemical markers.

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