Browsing by Author "Ahmadian, Elham"

Now showing 1 - 6 of 6

Citation - WoS: 3
Citation - Scopus: 6
Anticancer, Antioxidant and Antimicrobial Activity of Elaeagnus Angustifolia L. Leaf Extract
(Dove Medical Press Ltd, 2025) Do Gan, Serap; Mese, Ahmet; Baran, Mehmet Firat; Baran, Ayse; Aktepe, Necmettin; Ahmadian, Elham; Aktas, Husnu; Doğan, Serap
Objective: This study was conducted to determine and compare the antioxidant, cytotoxic, and antimicrobial effects of spindle leaves of Elaeagnus angustifolia L. (E. angustifolia) (oleaster) leaves. Methods: Total phenolic content was measured using the Folin-Ciocalteu method, phenolic compound analysis by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) and antimicrobial effect by the minimum inhibition concentration (MIC) method. The free radical scavenging activity was determined by the (2,2-Diphenyl-1-picrylhydrazyl) DPPH method, the free radical scavenging activity was determined by the ABTS method, and cytotoxicity assays were performed by the MTT method in human retinal epithelium cells (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer cells (DU-145) cell lines. Results: High amounts of gallic acid, protocatechuic acid, and o-coumaric acid were identified as phenolic compounds. E. angustifolia was found to have a good antioxidant capacity and high free radical scavenging capacity. In this study, for the first time, E. angustifolia leaf extract was used to investigate cytotoxic effects on human retinal epithelium (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer (DU-145) cells and antimicrobial effects on Listeria monocytogenes American Type Culture Collection (ATTC) 7644, Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 11774, and Candida albicans ATCC 10231 microorganisms. The highest cytotoxic effect was observed in the DU-145 cell line, and the highest antimicrobial effect was observed in Listeria monocytogenes ATTC 7644 and Candida albicans ATCC 10231. The leaf extract of the plant contains some important phenolic compounds and has high free radical scavenging capacity, a good anticancer effect, and effective antimicrobial activity on yeast species such as C. albicans. Conclusion: Our study will contribute greatly to the search for anticancer and antimicrobial agents, especially from a pharmacological perspective, by examining biological activity using three different methods.
Citation - WoS: 2
Citation - Scopus: 2
Antioxidant Properties of allium Turcicum Özhatay & Cowley Plant Extract, Its Effects on the Proliferation and Migration of Cancer Cells
(Frontiers Media Sa, 2024) Ipek, Polat; Baran, Ayse; Cebe, Deniz Baris; Ahmadian, Elham; Eftekhari, Aziz; Baran, Mehmet Firat
Cancer is a type of non-communicable disease that is responsible for numerous deaths worldwide. Cancer incidence and mortality rates are on the rise due to a combination of factors, such as a growing population, aging, and poor dietary habits. The Allium turcicum & Ouml;zhatay & Cowley plant is an endemic plant in the area where it grows and is consumed by the public due to its various benefits. This endemic plant, which generally grows in high-altitude regions, is sold in bunches because it is costly, mixed with rock salt, crushed into powder, and consumed as a spice. The cytotoxic and growth-inhibitory effects of A. turcicum & Ouml;zhatay & Cowley herb extract on human glioblastoma U373 cells, human colorectal carcinoma cell HCT-116, and healthy HUVEC cell lines were determined by the MTT method. After 24 and 48 h of application, logIC50 values in HUVEC, HCT-116, and U373 cells were defined as 3.737, 3.765; 3.513, 3.696, 4.476, and 4.104 mu g/mL, respectively. We conducted a cell migration experiment to study the A. turcicum & Ouml;zhatay & Cowley Extract (AT & Ouml;CE) impact on cancer cells' metastatic behavior. Our findings indicate that AT & Ouml;CE has an inhibitory effect on the migration potential of the cells used in the study. We conducted experiments using DPPH, ABTS, CUPRAC, and total phenolic content to assess the antioxidant properties of AT & Ouml;CE. The findings from the antioxidant activity experiments revealed an activity level of 0.20 +/- 0.046 at IC50. Additionally, the total phenolic content was measured to be 0.26 +/- 0.044 mg GAE/g.
Green Synthesis of Silver Nanoparticles From Elaeagnus Angustifolia Extract: Characterization and Evaluation of Antibacterial and Cytotoxic Properties
(Springer, 2026) Baran, Ayse; Ghorbanzadeh, Vajihe; Dogan, Yusuf; Ahmadian, Elham; Zulfugarova, Parvin; Mohamed, Ali Jimale
BackgroundCurrent medical problems are complex and require a new approach. Nanomaterials can address these complications. Silver nanoparticles (AgNPs), in particular green-synthetized particles, because of their unique properties have attract the attention of scientist. The objective of this work deals with using Elaeagnus angustifolia (EA) leaf extract as a reducing agent for biofabrication of AgNPs and investigation of its antibacterial and anti-cancer properties.MethodUV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), transmission Electron Microscopy (TEM), Scanning-Transmission Electron Microscope (STEM) and atomic force microscopy (AFM) techniques were used for characterization of the biosynthesized AgNPs. Antimicrobial efficacy was measured through disk diffusion and minimum inhibitory concentration (MIC) methods, while cytotoxic effects on PC-3 cancer cells were evaluated using the MTT assay.ResultThe biosynthesized AgNPs exhibited a strong surface plasmon resonance peak at approximately 441 nm, confirming successful synthesis. XRD analysis indicated a face-centered cubic structure, with crystallite sizes 27.04 nm. Antibacterial tests revealed significant activity against E. coli and K. pneumoniae, with AgNPs demonstrating comparable efficacy to standard antibiotics. In particular, AgNPs demonstrated successful activity on E. coli with an MIC value of 113.24 +/- 14.36 and an inhibition zone of 24.32 +/- 1.25 mm, comparable to standard antibiotics Furthermore, the AgNPs displayed notable cytotoxic effects on PC-3 cells, with an IC50 value of 58.77 mu g/mL.ConclusionThe results explore the potential of leaf extract of Elaeagnus angustifolia as an effective agent for the green synthesis of AgNPs that have significant antibacterial properties. This study supports the application of green synthesis in medical therapies.
Citation - WoS: 25
Citation - Scopus: 33
Green Synthesis, Characterization of Gold Nanomaterials using Gundelia tournefortii Leaf Extract, and Determination of Their Nanomedicinal (Antibacterial, Antifungal, and Cytotoxic) Potential
(Hindawi, 2022) Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Hatipoğlu, Abdulkerim; Adican, Mehmet Tevfik; Atalar, Mehmet Nuri; Huseynova, Irada; Khalilov, Rovshan; Ahmadian, Elham; Yavuz, Ömer; Kandemir, Sevgi İrtegün; Eftekhari, Aziz
Introduction. Fighting against cancer and antibiotic resistance are important challenges of healthcare systems, and developing new treatment methods has become the most concentrated area of researchers. Method and Materials. Green synthesis, characterization, and some biological activities of gold nanomaterials (AuNPs) obtained with Gundelia tournefortii (kenger) leaf extract were investigated in this study. Fourier scanning electron microscope, UV-visible spectrophotometer, Fourier transform ınfrared spectroscopy, energy-dispersive X-ray spectrophotometer, X-ray diffraction diffractometer, transmission electron microscope, and Zetasizer instrument data were used to elucidate the structures of nanoparticles. Results. The maximum surface plasmon resonance was observed at 532.15 nm after 1 hour. With the powder XRD model, the mean cubic crystallite size was determined as 23.53 nm. It was observed that the shapes of the obtained AuNPs were spherical, and the dimensions were 5-40 nm and hexagonal. Surface charges (-27 mV) and average size (365.3 nm) of gold nanoparticles were measured with a zeta analyzer. Conclusion. The suppressive effects of AuNPs on the growth of pathogenic microorganisms and healthy and cancer cell lines were determined using the MIC and MTT methods, respectively.
Citation - WoS: 54
Citation - Scopus: 64
Green-Synthesized Silver Nanoparticles From Peel Extract of Pumpkin as a Potent Radiosensitizer Against Triple-Negative Breast Cancer (tnbc)
(Springer Wien, 2024) Montazersaheb, Soheila; Eftekhari, Aziz; Shafaroodi, Amir; Tavakoli, Soodeh; Jafari, Sara; Baran, Ayse; Ahmadian, Elham
Background: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer. Radiation therapy (RT) is a modality for TNBC management. Radiosensitizers can mitigate the adverse effects of RT. Applying green-synthesized silver nanoparticles (Ag-NPs) from biological sources such as plants is a potential strategy to sensitize cancer cells to radiotherapy due to the low toxicity. Therefore, identifying novel natural sources for synthesizing stable and broadly applicable green-Ag-NPs has gained more attention in cancer therapy. In the present study, we synthesized green- Ag-NPs from pumpkin peel extract and elucidated the impact of green-synthesized Ag-NPs as a radiosensitizer in MDA-MB 231 cells (a model of TNBC). Results: The prepared Ag-NPs had a spherical shape with an average size of 81 nm and a zeta potential of - 9.96 mV. Combination of green-synthesized Ag-NPs with RT exhibited synergistic anticancer effects with an optimum combination index (CI) of 0.49 in MDA-MB-231 cells. Green-synthesized Ag-NPs synergistically potentiated RT-induced apoptosis in MDA-MB-231 cells compared to the corresponding monotherapies. Morphological features of apoptosis were further confirmed by the DAPI-TUNEL staining assay. HIF-1 alpha expression was decreased in cells subjected to combination therapy. Bax and p53 expression increased, whereas Bcl-2 genes decreased. Combination therapy significantly increased the protein level of PERK and CHOP while decreasing cyclin D1 and p-ERK/total ERK levels compared to monotherapies. Conclusion: These findings indicate the potential effect of green-synthesized Ag-NPs as a radiosensitizer for TNBC treatment.
Citation - WoS: 14
Citation - Scopus: 19
Synthesis and Characterization of Activated Carbon-Supported Magnetic Nanocomposite (mnps-Olac) Obtained From Okra Leaves as a Nanocarrier for Targeted Delivery of Morin Hydrate
(Frontiers Media Sa, 2024) Ozic, Cem; Ertas, Erdal; Baran, Mehmet Firat; Baran, Ayse; Ahmadian, Elham; Eftekhari, Aziz; Yildiztekin, Mahmut
Introduction The method of encapsulating the drug molecule in a carrier, such as a magnetic nanoparticle, is a promising development that has the potential to deliver the medicine to the site where it is intended to be administered. Morin is a pentahydroxyflavone obtained from the leaves, stems, and fruits of various plantsmainly from the Moraceae family exhibiting diverse pharmacological activities such as anti-inflammatory, anti-oxidant, and free radical scavenging and helps treat diseases such as diabetes, myocardial infarction and cancer.Methods In this study, we conducted the synthesis of a nanocomposite with magnetic properties by coating biocompatible activated carbon obtained from okra plant leaves with magnetic nanoparticles.Results Characterization of the synthesized activated carbon-coated magnetic nanocomposite was confirmed by Fourier transform infrared, scanning electron microscopy, dynamic light scattering, and zeta potential. The cytotoxic effects of the drug-loaded magnetic nanocomposite were examined in HT-29 (Colorectal), MCF-7 (breast), U373 (brain), T98-G (Glioblastoma) cancer cell lines, and human umbilical vein endothelial cells healthy cell line.Discussion We studied the loading and release behavior of morin hydrate in the activated carbon-coated magnetic nanocomposite. Activated carbon-coated magnetic nanocomposite carriers can show promising results for the delivery of Morin hydrate drugs to the targeted site.