Browsing by Author "Alhilal, Suzan"

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Citation - WoS: 1
Citation - Scopus: 1
Ascorbic Acid Exhibits More of a Protective Effect Than Estradiol Against Nephrotoxicity Induced by Malathion in Rats: a Histopathological and Molecular Docking Study
(Tubitak Scientific & Technological Research Council Turkey, 2025) Alhılal, Mohammad; Alhılal, Mohammad; Salem, Mahmoud Elsayed Mohamed; Alhılal, Suzan; Alhilal, Suzan; Albakoush, Ahmed; Farag, Bassant; M.gomha, Sobhi; 09.01. Department of Nursing / Hemşirelik Bölümü; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Background/aim: Despite the known harmful effects associated with malathion toxicity in various organs, it continues to be widely used for plant protection and insect control. This study is the first to compare the protective effects of estradiol and ascorbic acid against malathion-induced nephrotoxicity through histopathological assessment and molecular docking analyses. Materials and methods: This study was conducted using 20 female albino rats that were distributed into sham, malathion, malathion + estradiol, and malathion + ascorbic acid groups. Nephrotoxicity was induced by daily treatment with malathion and the effects of estradiol and ascorbic on nephrotoxicity were evaluated. After 4 weeks of treatment, the animals were sacrificed and the kidneys were examined following hematoxylin and eosin (H&E) staining. Histopathology results were supported by molecular docking studies of estradiol and ascorbic acid against a target protein (PDB ID: 2YMX), the peptide inhibitor Fab408 inhibiting acetylcholinesterase (AChE). The inhibition of AChE is the primary mechanism of the toxic effects of malathion. Results: Histopathological examination revealed a notable elevation (p < 0.001) in degeneration and necrosis within the tubular epithelium and interstitial nephritis in the malathion group compared to the sham group. Daily administration of estradiol and ascorbic acid resulted in a notable reduction (p = 0.0022) in the severity of these histopathological changes in the malathion + estradiol and malathion + ascorbic acid groups compared to the malathion group. Of these, the most significant decreases were observed in the malathion + ascorbic acid group. Docking studies of these compounds against the selected protein (PDB ID: 2YMX) revealed promising binding scores. Ascorbic acid exhibited the highest docking score (–6.44 kcal/mol), indicating a favorable binding interaction with this protein. Conclusion: Estradiol and ascorbic acid exert protective effects against malathion-induced nephrotoxicity, whereas ascorbic acid showed superior efficacy compared to estradiol. This result was further supported by molecular docking studies.
Biological Evaluation and Molecular Docking Studies of Novel Aza-Acyclic Nucleosides as Putative Antimicrobial, Anticancer, and Antioxidant Agents
(BMC, 2025) Alhilal, Mohammad; Alhilal, Suzan; Gomha, Sobhi M.; Farag, Basant; Sabancilar, Ilhan; Ouf, Salama A.
This study aimed to synthesize new aza-acyclic nucleosides (aza-acyclovir) and evaluate the efficacy of these synthetic compounds as potential antimicrobial, anticancer, and antioxidant agents. We prepared two novel aza-acyclic nucleosides via two reactions. The first reaction involved trichloroisocyanuric acid and dibenzosulphonyl diethylamine, and the second reaction involved trichloroisocyanuric acid and diethanolamine. We then used one-dimensional nuclear magnetic resonance (NMR) spectroscopy, two-dimensional NMR spectroscopy, infrared spectroscopy, and mass spectrometry to determine the structures of the resulting compounds. In this regard, we first tested the antimicrobial activity of these compounds against various bacteria, including Bacillus cereus, B. subtilis, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa, and against fungal pathogens, including Aspergillus fumigatus, Candida tropicalis, and Alternaria solani. Next, the precise mode for the interaction between synthesized aza-acyclic nucleosides and the target protein 8HQ5 was elucidate using molecular docking analysis. Subsequently, we tested the synthesized compounds for putative anticancer activity at different concentrations (i.e., 12.5, 25, 50, 100, and 200 mu g/mL) against A549 cell (Human epithelial lung carcinoma) and human umbilical vein endothelial cell (HUVEC) lines. In addition, compounds antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl-based and cupric reducing antioxidant capacity-based methods at different concentrations (i.e., 31.25, 62.5, 125, 250, and 500 mu g/mL). Results revealed that both aza-acyclic nucleosides inhibited both bacterial and fungal strains, although toxicity toward bacterial strains was generally greater than toward fungal strains. We also observed that the molecular docking results were consistent with the results of in vitro antimicrobial assessments. Further, both aza-cyclic nucleosides exhibited cytotoxic effects against both the A549 cell and HUVEC lines. Despite exhibiting lower radical scavenging activity than ascorbic acid (an antioxidant compound used as a standard), Compound 1 from the novel synthetic aza-acyclic nucleosides showed a higher reduction capacity, which was dose-dependent. Overall, we report newly synthesized compounds that show promising antimicrobial, anticancer, and antioxidant effects.
Citation - WoS: 1
Citation - Scopus: 1
Isocarpine as A New Peperidine Alkaloid from Adenocarpus Complicatus with Anticandidal Activity
(Ingenta Connect, 2022) Alhilal, Mohammad; Alhılal, Mohammad; Alhilal, Suzan; Alhılal, Suzan; Gomha, Sobhi; 09.01. Department of Nursing / Hemşirelik Bölümü; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Background: Adenocarpus complicatus L. is one of Adenocarpus genus, which has about 50 species, from Fabaceae (Papilionaceae) family. This plant is found in the Mediterranean basin as a southern west part of Europe and North Afric forestes. Objective: Isolation of Isocarpine From Adenocarpus Complicatus as Anticandidal Agent. Methods: A new piperidine alkaloid Isocarpine was isolated from the aerial parts of Adenocarpus complicatus L., which is gross in Syria. The structure of the new compound was determined by UV, IR, EI-Ms, 1H-NMR, 13C-NMR, DEPT-135, DQF-COSY and HMQC. Results: The anticandidal activity of isocarpine was screened against 38 Candida strains and showed remarkable inhibitory effect compared with fluconazole as an antifungal reference drug. Conclusion: Isocarpine is antifungal agent.
Medicinal Evaluation and Molecular Docking Study of Osajin as an Anti-Inflammatory, Antioxidant, and Antiapoptotic Agent Against Sepsis-Associated Acute Kidney Injury in Rats
(Taylor & Francis Ltd, 2024) Alhilal, Mohammad; Erol, Huseyin Serkan; Yildirim, Serkan; Cakir, Ahmet; Koc, Murat; Alhilal, Suzan; Halici, Mesut Bunyami
Despite efforts to find effective drugs for sepsis-associated acute kidney injury (SA-AKI), mortality rates in patients with SA-AKI have not decreased. Our study evaluated the protective effects of isoflavone osajin (OSJ) on SA-AKI in rats by targeting inflammation, oxidative stress, and apoptosis, which represent the cornerstones in the pathophysiological mechanism of SA-AKI. Polymicrobial sepsis was induced in rats via the cecal ligation and puncture (CLP) technique. Markers of oxidative stress were evaluated in kidney tissues using biochemical methods. The expression of interleukin-33 (IL-33), 8-hydroxydeoxyguanosine (8-OHdG), caspase-3, and kidney injury molecule-1 (KIM-1) was evaluated as indicators of inflammation, DNA damage, apoptosis, and SA-AKI respectively in the kidney tissues using immunohistochemical and immunofluorescent detection methods. The CLP technique significantly (p < 0.001) increased lipid peroxidation (LPO) levels and significantly (p < 0.001) decreased the activities of superoxide dismutase and catalase in kidney tissues. In the renal tissues, strong expression of IL-33, 8-OHdG, caspase-3, and KIM-1 was observed with severe degeneration and necrosis in the tubular epithelium and intense interstitial nephritis. In contrast, the administration of OSJ significantly (p < 0.001) reduced the level of LPO, markedly improved biomarkers of antioxidant status, decreased the levels of serum creatinine and urea, lowered the expression of IL-33, 8-OHdG, caspase-3, and KIM-1 and alleviated changes in renal histopathology. A promising binding score was found via a molecular docking investigation of the OSJ-binding mode with mouse IL-33 (PDB Code: 5VI4). Therefore, OSJ protects against SA-AKI by suppressing the IL-33/LPO/8-OHdG/caspase-3 pathway and improving the antioxidant system.
Citation - WoS: 25
Citation - Scopus: 25
Novel thiadiazole-thiazole hybrids: synthesis, molecular docking, and cytotoxicity evaluation against liver cancer cell lines
(Taylor & Francis Online, 2022) Aljohani, Ghadah F.; Alhılal, Mohammad; Abolibda, Tariq Z.; Alhılal, Suzan; Alhilal, Mohammad; Al-Humaidi, Jehan Y.; Alhilal, Suzan; Ahmed, Hoda A.; Gomha, Sobhi M.; 09.01. Department of Nursing / Hemşirelik Bölümü; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
One of the worst diseases, cancer claims millions of lives each year throughout the world, necessitating the creation of novel treatments. In this study, we designed a novel series of 1,3,4-thiadiazoles through the reaction of 2-(4-methyl-2-(2-(1-phenylethylidene)hydrazineyl)thiazole-5-carbonyl)-N-phenylhydrazine-1-carbothioamide (3) with the proper hydrazonoyl halides. Using the MTT assay, the newly synthesized thiadiazoles' growth-inhibitory potential against the liver cancer cell line HepG2-1 was assessed. In comparison to the standard drug doxorubicin (IC50 = 0.72 ± 0.52 µM), the results showed that two compounds, 16b and 21 (IC50 = 0.69 ± 0.41 and 1.82 ± 0.94 µM, respectively) had promising anticancer activity. The structural activity relationship (SAR) was investigated. In addition, molecular docking analysis onto quinone oxidoreductase2 (NQO2) receptor (PDB: 4ZVM) was investigated against the potent compounds to examine the reliability of the in vitro results. The newly prepared thiadiazole-thiazole hybrids are therefore regarded as potent anticancer drugs.
Osajin is a Promising Candidate for Sepsis-Induced Brain Damage via Suppression of the 8-OHdG/Bax/Caspase-3 Pathway in a Rat Model of Sepsis
(2025) Erol, Huseyin Serkan; Halıcı, Mesut; Koç, Murat; Alhılal, Mohammad; Yildirim, Serkan; Kiliçlioğlu, Metin; Alhilal, Suzan
Amaçlar: Doğal ürün olan osajinin, sepsis kaynaklı beyin hasarına karşı koruyucu etkisini, sepsisli sıçanların beyin dokusundaki 8-hidroksideoksiguanozin (8-OHdG)/Bcl-2 ile ilişkili × protein (Bax)/kaspaz-3 yolunu hedef alarak inceledik. Metotlar: Osajin, Maclura pomifera'nın meyvesinden izole edilip yapısı doğrulandı. Çekal ligasyon-punksiyon (CLP) yöntemi ile sıçanlarda beyin hasarı modeli oluşturuldu. Osajin, sepsise bağlı beyin hasarı olan hayvanlara 150 ve 300 mg/kg dozlarda uygulanmıştır. Ötenazi sonrasında histopatolojik inceleme, immünohistokimya ile 8-OHdG'nin tespiti ve immünfloresan teknik kullanılarak Bax ve kaspaz-3 ekspresyonunun tahmini beyin dokusunda yapıldı. Bulgular: Histopatolojik incelemede sepsisli sıçanların beyinlerinde şiddetli düzeyde inflamasyon, belirgin dejenerasyon ve nekroz görüldü. İmmünohistokimyasal ve immünfloresan analizlerin bulguları, CLP tekniğinin, sağlıklı sıçanlarla karşılaştırıldığında sepsisli sıçanların beyin dokularında belirgin 8-OHdG, Bax ve kaspaz-3 ekspresyonunu indüklediğini ortaya çıkardı. 150 mg/kg (p < 0.05) ve 300 mg/kg (p = 0.0022) dozlarda osajin'in uygulanması, histopatolojik değişiklikleri tersine çevirdiği ve sepsisli sıçanlarla kıyaslandığında artan 8-OHdG, Bax ve kaspaz-3 ekspresyonunu önemli ölçüde iyileştirdiği gözlendi. Sonuç: Histopatolojik, immünohistokimyasal ve immünfloresan kanıtlar, osajin'in, 8-OHdG/Bax/kaspaz-3 yolunu inhibe ederek sepsisin neden olduğu beyin hasarını tersine çevirebileceğini göstermektedir. Buna göre bu doğal ürünün, sepsisli hastalardaki beyin hasarının tedavisi için umut verici bir aday olabileceği gösterilmiştir.
Citation - WoS: 9
Citation - Scopus: 8
Synthesis and biological evaluation of new aza-acyclic nucleosides and their hydrogen complexes from indole
(SpringerLink, 2022) Alhilal, Suzan; Alhılal, Suzan; Alhilal, Mohammad; Alhılal, Mohammad; Gomha, Sobhi M.; Ouf, Salama A.; 09.01. Department of Nursing / Hemşirelik Bölümü; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Three novel aza-acyclic nucleosides and two hydrogen complexes were isolated by flash chromatography after being produced in a reaction between indole and dibenzosulfonyl diethylamine (which had previously been prepared) in the presence of sodium and absolute ethanol as a basic catalyst. Structures of new compounds and complexes were determined by 1D-NMR: 1H NMR, 13C NMR, DEPT-135, 2D-NMR: COSY, HMQC, HSQC, HMBC, IR, and MS spectroscopy. The synthesized compounds were evaluated against a wide range of microorganisms, including Gram-positive and Gram-negative bacteria as well as fungal strains. These compounds showed good biological activity.
Citation - WoS: 6
Citation - Scopus: 5
Synthesis of Novel Acyclic Nucleoside Analogue Starting From 6-Aminouracil as Potent Antimicrobial Agent
(Polycyclic Aromatic Compounds, 2021) Alhilal, Mohammad; Alhılal, Mohammad; Sulaiman, Yaser A. M.; Alhılal, Suzan; Alhilal, Suzan; Gomha, Sobhi M.; Ouf, Salama A.; 09.01. Department of Nursing / Hemşirelik Bölümü; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
6-Aminouracil and 2-bromoethyl amine were prepared, as starting materials to be introduced as an alkylating reagent with sodium carbonate as a catalyst. Acyclic nucleoside was prepared for the first time, the expected structure of the final new compound 3 was determined based on IR, NMR, and mass spectroscopy, with safe and mild reaction conditions. The synthesized acyclic nucleoside has a potent and efficient antimicrobial activity compared to reference drugs particularly as an antibacterial agent, and can be used as an alternative to the commonly used antibiotics after performing the necessary biological research for its validation.