Browsing by Author "Baysal, Zubeyde"

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Docetaxel-Loaded, Dipeptide-Functionalized Γ-Fe2o3 Magnetic Nanoparticles: Synthesis, Characterization, and Targeting To Prostate Cancer
(Wiley-v C H verlag Gmbh, 2025) Gungoren, Muhammed; Koyuncu, Ismail; Kilinc, Ersin; Baysal, Zubeyde
A new delivery system was designed and synthesized to increase the efficiency of docetaxel. For this aim, gamma-Fe2O3 was synthesized in order to give the nanoparticle the ability to be magnetic targeted. It was functionalized with citric acid to prevent clumping and maintain stability. To increase the interaction efficiency between the delivery system and the drug, leucine-glycine dipeptide and an amphiphilic polymer, Pluronic F127, were attached to the nanoparticle. Characterization studies were carried out with XRD, ICP-MS, vibrating sample magnetometer (VSM), TEM, and zeta potential to elucidate the structure of this carrier system. After docetaxel was bound to the synthesized carrier, its therapeutic effect was measured by spectrophotometric and flow cytometric methods (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT], reactive oxygen species [ROS], and apoptosis). Docetaxel-bound nanoparticles significantly reduced the viability of the tumor cell compared to the healthy cell in a dose-dependent manner, especially at 24 (50.14 +/- 13.25 to 22.32 +/- 5.51 at 100 mu g/mL) h and 48 (41.46 +/- 2.22 to 18.80 +/- 2.81 at 100 mu g/mL) h. The rate of apoptosis and ROS in the negative sample increased from 2.7% to 15.4% and from 9.6% to 74.7%, respectively, as a result of treatment with 15 mu g/mL gamma-Fe2O3/C/LG/F127/Dotx. Thus, it can be said that the synthesized carrier system is a suitable candidate for the improvement of docetaxel transport.
Preconcentration with Bacillus subtilis-Immobilized Amberlite XAD-16: Determination of Cu2+ and Ni2+ in River, Soil, and Vegetable Samples
(TAYLOR & FRANCIS INC, 2015) Okumus, Veysi; Ozdemir, Sadin; Kilinc, Ersin; Dundar, Abdurrahman; Yuksel, Uyan; Baysal, Zubeyde
Solid-phase extraction (SPE) method was developed for the preconcentration of Cu2+ and Ni2+ before their determination by inductively coupled plasma optical emission spectrometry (ICP-OES). Bacillus subtilis-immobilized Amberlite XAD-16 was used as biosorbent. Effects of critical parameters such as pH, flow rate of samples, amount of Amberlite XAD-16 and biosorbent, sample volume, eluent type, and volume and concentration of eluent on column preconcentration of Cu2+ and Ni2+ were optimized. Applicability of the method was validated through the analysis of the certified reference tea sample (NCS ZC73014). Sensitivity of ICP-OES was improved by 36.4-fold for Cu2+ and 38.0-fold for Ni2+ by SPE-ICP-OES method. Limit of quantitation (LOQ) was found to be 0.7 and 1.1ng/ml for Cu2+ and Ni2+, respectively. Concentrations of Cu2+ and Ni2+ were determined by ICP-OES after application of developed method. Relative standard deviations (RSDs) were lower than 4.9% for Cu2+ and 7.9% for Ni2+. The Tigris River that irrigates a large agricultural part of Southeast Turkey is polluted by domestic and industrial wastes. Concentrations of Cu2+ and Ni2+ were determined in water, soil, and some edible vegetables as a biomonitor for heavy metal pollution.