Browsing by Author "Cetik Yildiz, Songul"

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Cardioprotective effects of Hypericum triquetrifolium Turra. against cyclophosphamide related cardiotoxicity in rats
(2018) Çetik Yıldız, Songül; KESKİN, Cumali; Sahinturk, Varol; Ayhanci, Adnan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
ABSTRACT: Cyclophosphamide (CYP) is commonly used as anticancer agent but its usage is limited by cardiotoxic side effects such as dose-dependent cardiac damage, morphologically defined necrosis and bleeding. Hypericum triquetrifolium Turra. (HT) shows anti-oxidative and anticarciogenic properties with its rich phenolic contents. The current study was designed to investigate the possible protective effect of HT on CYP-induced cardiotoxicity. Albino rats were randomly divided into 9 groups, each included 7 animals. Serum creatine kinase-MB (CK-MB), malondialdehyde (MDA), aspartate transaminase (AST), glutathione (GSH), total antioxidant (TAC) and total oxidant capacity (TOC) levels were investigated. Furthermore, the cardiac tissue samples were investigated histopatologically. While the levels of serum CK-MB, MDA, AST and TOC were high, the levels of serum GSH and TAC levels were low in the CYP groups. It was also observed that CYP-induced cardiotoxicity was dose dependent. In the treatment with CYP plus HT doses there was observed an essential decrease in the CYP cardiotoxicity; decreased cell damage and oxidative stress parameters and also increased GSH and TAC levels. Based on our findings, it can be proposed that HT seed methanol extract was a strong candidate in preventing the CYP-induced cardiotoxicity.
Citation - WoS: 56
Citation - Scopus: 60
Cyclophosphamide induced oxidative stress, lipid per oxidation, apoptosis and histopathological changes in rats: Protective role of boron
(Elsevier, 2020) Çetik Yıldız, Songül; Şahintürk, Varol; Cetik Yildiz, Songul; Kurcanay Şahin, İlknur; Bilici, Namık; Onur Yaman, Suzan; Altuner, Yılmaz; Appak-Baskoy, Sıla; Ayhanci, Adnan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Background Cyclophosphamide (CP) is an alkylating chemotherapeutic drug used in the treatment of many types of cancer. However, as with other chemotherapeutic drugs, the use of CP is limited by the damage to healthy tissues such as testes, bladder and liver as well as cancerous tissue. Boron (B) is a trace element with many biological properties such as antioxidant, anti-apoptotic and anti-lipid per oxidation. Methods This current study aims to determine protective effects of B on CP induced testicular toxicity. The rats were divided into 4 groups (control, CP, B and B plus CP groups). The testes of experimental animals were taken for histological, apoptotic markers and biochemical analysis. Results The damage to some seminifer tubules, loss of typical appearance, thinning of seminifer epithelium and relative enlargement of the tubule lumen were watched in testis of the group that administrated CP. Moreover, Bcl-2, TAC and GSH levels decreased while TOC, OSI, MDA, Bax and Caspase-3 levels increased. On the other hand, pretreatment limited to B in the B plus CP group, testicular tissue improved. In addition, Bcl-2, GSH, TAC levels increased, Bax, MDA, TOC, OSI and caspase-3 levels decreased. Conclusion B significantly reduced testicular lipid per-oxidation and strengthened antioxidant defenses. Our results showed that pre-treatment B can protect rat testis against CP-induced testicular damage owing to its anti-lipid per oxidation, anti-oxidant and anti-apoptotic properties.
Evaluation of the Serological, Biochemical and Hemogram Parameters of Patients Prediagnosed with Hydatid Cyst
(2021) Demir, Cemil; Çetik Yıldız, Songül; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Objective: Hydatid cyst or echinococcosis is a zoonotic parasitic disease caused by the larval stages of Echinococcus granulosus, which is common in our country. It is necessary to use radiological and serological methods as diagnosing this disease based on clinical symptoms is very difficult. In this retrospective study, it was aimed to determine the condition in our region by retrospectively analyzing the hemogram, biochemistry, radiological and pathological findings and the epidemiological data of the patients prediagnosed with hydatid cyst. Materials and Methods: A total of 412 patients, 279 female and 133 male, who applied to various clinics of the Mardin State Hospital with hydatid cyst prediagnosis. 52 patients were diagnosed with hydatid cyst based on ultrasonography, computerized tomography and radiological findings, and liver involvement was detected in all 64 patients, 12 of which were pathologically verified. Results: 38 (59.3%) of 64 patients included in the study were female, 26 (40.6%) were male and their ages ranged from 0-77. The most common symptoms were severe abdominal pain, distention, chest pain, shortness of breath, 415 cough, fever, nausea and vomiting. 83.4% of the patients applied to the general surgery, 5.5% applied to the pediatric surgery and 10.1% applied to other clinics. Conclusion: The parameter with the highest increase was GGT, followed by ALT, AST and ALP. Elevated RDW (29%) was the most common finding in the hemogram parameters, followed by decreased MCV, hematocrit and hemoglobin.
Investigation of nasal Staphylococcus aureus carriage by real-time PCR in patients receiving hemodialysis treatment
(2023) Demir, Cemil; Çetik Yıldız, Songül; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
ABSTRACT Objectives: Staphylococcus aureus, coagulase-negative staphylococci (CoNS), and methicillin-resistant S. aureus (MRSA), which are significant nosocomial pathogens, have become a growing global problem because their carriage and diseases have become resistant to many antibiotics. This study aimed to investigate and determine the rate of MRSA carriage among patients receiving hemodialysis treatment using molecular methods. Methods: In the 254 hemodialysis patients, the nasal carriage rates, susceptibility and resistance to S. aureus, CoNS and MRSA were examined using culture and real-time PCR methods. Nasal samples from hemodialysis patients were examined using real-time PCR. Microscopic examination was performed using the Gram staining method, and S. aureus was identified using catalase and coagulase. The strains were then tested for antibiotic susceptibility. Staphylococci was isolated from 231 of the 254 patients. Results: S. aureus carriage was detected in 50 patients, MRSA in 16, methicillin-susceptible S. aureus (MSSA) in 33, CoNS in 66, methicillin-resistant CoNS (MR-CoNS) in 38, and methicillin-susceptible CoNS (MSCoNS) in 28. S. aureus and MRSA strains exhibited 100% susceptibility to nitrofurantoin, and vancomycin. MSSA strains showed the highest susceptibility to chloramphenicol, clindamycin (84.8%), and co-trimoxazole (36.4%). CoNS showed 100% susceptibility to vancomycin, and 16.7% susceptibility to ampicillin. Vancomycin was found to be the most effective antibiotic against S. aureus, CoNS, and MRSA pathogens identified in patients undergoing hemodialysis, whereas penicillin resistance was found. Conclusions: It can be concluded that one of the most effective ways to prevent the formation of antibiotic resistant strains is the hygiene of the hospital and hospital staff. Real-time PCR is very important for analyzing with high sensitivity.
Myeloprotective and hematoprotective role of kefir on cyclophosphamide toxicity in rats
(2021) Çetik Yıldız, Songül; Cetik Yildiz, Songul; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Abstract Aim: Kefir is a probiotic and prebiotic beverage produced from milk and kefir grains containing a mixture of bacteria and yeast. Drugs like cyclophosphamide (CPx) that are used for cancer chemotherapy are generally limited due to numerous unwanted side-effects such as multiple organ toxicity. For this purpose, the cell-protective effects of kefir, a natural probiotic known for its antitumor and antioxidant properties, on CPx-induced hemotoxicity and myelotoxicity were investigated in this study. Methods: Group 1 (control, 0.5 ml SF). Group 2 were administered a single dose of 150mg/kg CPx. Group 3 and 5 were given 5 and 10mg/kg kefir. Group 4 and 6 were given 5 and 10mg/kg kefir+150mg/kg CPx. While kefir was administered to the rats by gavage method for 12 days, CPx was administered as single-dose on the 12th day. Results: The DPPH results showed that kefir possesses high antioxidant activity. It was observed that the leukocytes, thrombocytes, erythrocytes, hemoglobin, hematocrit and bone marrow nucleated cell levels decreased in the group that was administered only CPx, and increased relatively in the groups that were administered CPx+kefir, drawing close to the control. Conclusion: The results of the present study showed that kefir had antioxidant and cytoprotective activity, protecting blood and bone marrow cells against CPx-induced damage.
Presence of biofilm and adhesin genes in Staphylococcus aureus strains taken from chronic wound infections and their genotypic and phenotypic antimicrobial sensitivity patterns
(2020) Demir, Cemil; Çetik Yıldız, Songül; Yigin, Akin; Tokman, Hrisi Bahar; Cetik Yildiz, Songul; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
The purpose of this research was to examine biofilm (icaA, icaD and bap) and adhesin (clfA, fnbA, cna) genes, and also assess the genotypic and phenotypic antimicrobial resistance patterns of Staphylococcus aureus strains taken from wound specimens in Mardin, Turkey. A total of 220 wound specimens were investigated. The biofilm forming ability and resistance pattern for eleven antimicrobial agents were investigated by conventional and multiplex PCR methods. S. aureus were taken from 112 (50.9%) of 220 wound specimens. Moreover, biofilm production was found in 79 (70.5%) of the 112 S. aureus isolates. 97 (86.6%) strains of all isolates were positive for icaA and icaD, and 15 (13.4%) for bap. The adhesin genes, cna, fnbA and clfA were detected in 98 (87.5%), 87 (77.7%), and 75 (66.9%) strains, respectively. The numbers of MSSA and MRSA bearing antimicrobial resistance genes were 19 (16.96%) and 32 (28.57%) for blaZ, 9 (8.04%) and 17 (15.18%) for tetK, 6 (5.36%) and 14 (12.5%) for ermC, 2 (1.79%) and 7 (6.25%) for tetM, 0 (0%) and 5 (4.46%) for mecA, 2 (1.79%) and 4 (3.57%) for ermA, 1 (0.89%) and 2 (1.79%) for both tetK and tetM, respectively. Our findings indicate that multiplex PCR is a suitable way for identifying biofilm and adhesin producing S. aureus. Our data also provided a country-wide oversight of the S. aureus antimicrobial resistance gene profiles for the properly therapy of patients and to control the spreading of the resistance genes.