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Browsing by Author "Cicek, Mustafa"

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    Morin Attenuates Arsenic-Induced Toxicity in 3t3 Embryonic Fibroblast Cells by Suppressing Oxidative Stress, Inflammation, and Apoptosis: in Vitro and Silico Evaluations
    (Oxford Univ Press, 2024) Unsal, Velid; Cicek, Mustafa; Aktepe, Necmettin; Oner, Erkan
    This study aims to investigate the curative effects of Morin, a flavonoid, against arsenic toxicity in 3T3 embryonic fibroblast cells and its effect on the molecular mechanisms of cells. The cytotoxicity and viability of the cells were measured by MTT and LDH tests. Arsenic (0.74 mu M) was used to trigger toxicity and Morin (50 mu M) was used for treatment. The levels of oxidative stress biomarkers and the activities of antioxidant enzymes were measured by spectrophotometric method, and inflammatory markers were measured by ELISA method. While mRNA expression levels of Bax, Bcl-2 levels, and Caspase-3 activity were measured by qRT-PCR technique, TUNEL staining was performed to detect DNA breaks and DAPI staining to visualize nuclear changes. Protein structures were retrieved from the protein data bank. OpenBabel and Autodock programs were used for the molecular docking study. Morin rescued the 3T3 embryonic fibroblast cells exposed to arsenic. However, Arsenic decreased the activities of antioxidant enzymes in cells and significantly increased oxidative stress, inflammation, and apoptosis. Morin treatment reduced oxidative damage and TNF-alpha and IL-1 beta levels. Arsenic-induced Caspase-3 mRNA expression level and Bax protein mRNA expression level were significantly increased, while Bcl-2 mRNA expression level was significantly decreased. While Caspase-3 mRNA expression level and Bax protein mRNA expression level decreased with morin treatment, Bcl-2 mRNA expression level increased significantly. Molecular docking study results showed good binding affinity of morin in SOD, GSH-Px, Bax, Bcl-2, Caspase-3, TNF-alpha, and IL-1 beta structures. Morin showed antioxidant, anti-inflammatory, and anti-apoptotic effects against Arsenic-induced cellular toxicity. Graphical Abstract
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    Trans-Chalcone Attenuate Arsenic-Induced Toxicity in 3t3 Embryonic Fibroblast Cells; An In Vitro And In Silico Study
    (Elsevier, 2024) Unsal, Velid; Yildiz, Resit; Cicek, Mustafa; Gungor, Meltem; Kurutas, Ergul Belge; Yıldız, Reşit
    This study investigated the curative role of trans-chalcone, a flanovide, against arsenic toxicity using in vitro and computer-based analyses. MTT and LDH methods were used to assess the cytotoxicity and viability of cells, spectrophotometric methods to evaluate SOD, GSH-px, MDA and PC biomarkers, and ELISA method to evaluate TNF-a IL-1(3 levels. Bax, Bcl-2 levels and Caspase-3 activity were measured by qRT-PCR technique, while TUNEL staining was performed to detect DNA breaks and DAPI staining was performed to visualize nuclear changes. In addition, computer-based analyses of trans-chalcone and Dimercaprol molecules were analyzed using SwissADME, ADMETlab, DFT web tools. Trans-chalcone treatment rescued 3T3 embryonic fibroblast cells, reduced oxidative stress. Again, trans-chalcone treatment showed positive effects on TNF-alpha, IL-1(3 levels and apoptotic markers. In conclusion, trans-chalcone showed antioxidant, anti-inflammatory, and anti-apoptotic effects against cellular toxicity caused by arsenic, as well as DFT, ADMET, drug similarity, molecular docking profiles predicted trans-chalcone to be a promising ligand. This research, based on in vitro and in silico analyses, may be useful in the development of promising drug(s) to reduce toxicity.