Browsing by Author "Cuce, Gokhan"

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Citation - WoS: 1
Citation - Scopus: 1
The Ameliorative Effects of Hesperidin in Rats Developed Hepatotoxicity With Deltamethrin
(Mashhad Univ Med Sciences, 2025) Karabekir, Seda Cetinkaya; Sozen, Mehmet Enes; Ayan, Ilknur Cinar; Savas, Hasan Basri; Cuce, Gokhan; Kalkan, Serpil
Objective(s): Deltamethrin (DLM) is a widely used insecticide in agriculture; however, exposure to it can lead to serious health problems. This study aimed to evaluate the protective effects of hesperidin (HSP), a natural antioxidant, against DLM-induced liver toxicity. Materials and Methods: Thirty-two male Wistar Albino rats (250-300 g, 4 months old) were divided into four groups. The control group received 1 ml of corn oil via oral gavage for 30 days. The DLM group received 1.28 mg/kg DLM in corn oil for 30 days. The DLM+HSP 100 mg/kg and DLM+HSP 300 mg/kg groups received 1.28 mg/kg DLM followed by 100 mg/kg or 300 mg/kg HSP in distilled water, respectively, 30 min after DLM administration for 30 days. Liver tissues were examined histopathologically. Masson's trichrome staining and PCR assessed fibrosis. Caspase 3 and 9 expressions in liver tissues were determined by immunohistochemistry and PCR. Biochemical analyses were conducted on serum samples. Results: HSP supplementation led to a dose-dependent decrease in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. DLM exposure decreased antioxidant capacity, while HSP supplementation increased it dose-dependently. Histopathological evaluations showed increased liver damage in the DLM group, while HSP administration reduced liver toxicity. Masson's trichrome staining and analysis of collagen I (COL1A1) and collagen III (COL3A1) gene expression revealed increased fibrosis in the DLM group, which was attenuated with HSP treatment. Conclusion: The potential prevention of DLM-induced liver toxicity and apoptosis by HSP may be an alternative protective strategy.
Citation - WoS: 3
Citation - Scopus: 3
Effect of Carvacrol on Diabetes-Induced Oxidative Stress, Fibrosis and Apoptosis in Testicular Tissues of Adult Rats
(Acad Sciences Czech Republic, Inst Physiology, 2025) Gultekin, Burcu; Cetinkaya Karabekir, Seda; Cinar Ayan, Ilknur; Savas, Hasan Basri; Cuce, Gokhan; Kalkan, Sabiha Serpil
Diabetes mellitus (DM) is a chronic and widespread disease that negatively affects the male reproductive system. Carvacrol (CAR), a naturally occurring flavonoid in plants, exhibits various biological and pharmacological activities, including antiinflammatory, antioxidant, and anticancer properties. This study aimed to investigate the potential effects of CAR on testicular tissue damage induced by diabetes, which was modeled by Streptozotocin (STZ) administration. Thirty-two male Wistar albino rats were divided into four groups: Group 1: Control (n=8), Group 2: DM (n=8), Group 3: DM+DMSO (0.1 % dimethyl sulfoxide) (n=8), and Group 4: DM+CAR (20 mg/kg) (n=8). Diabetes was induced by a single intraperitoneal STZ injection (50 mg/kg). Histological changes were assessed using Hematoxylin-Eosin (H&E) staining and the Johnsen scoring system. Apoptosis was evaluated through immunohistochemical staining for the mitochondrial apoptosis markers Bax and Bcl-2, as well as RT-qPCR analysis of their gene expression levels. Fibrosis assessment involved Masson-Trichrome staining and RT-qPCR analysis of mRNA levels for the COL1A1 and COL3A1 genes. Additionally, Total Oxidant Status (TOS), Total Antioxidant Status (TAS), Oxidative Stress Index (OSI), and C-Reactive Protein (CRP) levels were measured in testicular tissue. CAR treatment significantly improved histological alterations associated with diabetes-induced testicular damage. DM was found to increase Bax levels while reducing Bcl-2 levels, whereas CAR reduced Bax levels and increased Bcl-2 gene and protein expression. TOS and OSI levels were elevated in the DM group, whereas TAS levels increased in the DM+CAR group. No significant differences in CRP levels were observed between the groups. These findings suggest that CAR may be effective in mitigating diabetes-induced testicular damage.
Citation - WoS: 12
Citation - Scopus: 12
Protective Effect of Astaxanthin on Histopathologic Changes Induced by Bisphenol a in the Liver of Rats
(Univ Agriculture, Fac veterinary Science, 2024) Karabekir, Seda Cetinkaya; Gultekin, Burcu; Ayan, Ilknur Cinar; Savas, Hasan Basri; Cuce, Gokhan; Kalkan, Serpil
Bisphenol A (BPA) has several potential uses, including in polycarbonate plastics and epoxy resins, which could expose humans to it. Recognized for its hepatotoxicity and ability to accumulate in organs. We prompted this study to explore the hepatoprotective potential of astaxanthin (ASTX), an antioxidant against BPA toxicity. We used 32 male Wistar Albino rats and randomly assigned them as: Control, Sham (olive oil), BPA, and BPA+ASTX. At the end of the experiment, Native Thiol, Total Thiol, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured in serum samples. Histopathological scoring was performed to evaluate the changes caused by ASTX in the liver. Caspase 3 and caspase 9 expression in liver tissues was demonstrated immunohistochemically and by PCR. Collagen I (COL1A1) and collagen III (COL3A1) mRNA levels were measured by PCR in the tissue samples. The BPA group showed elevated AST and ALT with decreased Thiol levels. ASTX administration reversed these changes as observed by reduced AST and ALT levels and increased Thiol levels. Histopathology indicated increased liver damage and fibrosis in the BPA group which were alleviated in the BPA+ASTX group. Gene expression analyses revealed upregulated COL1A1 and COL3A1 in BPA, which was downregulated with ASTX. Immunohistochemistry and PCR confirmed BPA-induced caspase 3 and caspase 9 expression, which were attenuated by ASTX. This study underscores ASTX's hepatoprotective efficacy against BPA-induced hepatotoxicity which ultimately attributed to its antioxidant and antiapoptotic properties. Consequently, ASTX emerges as a promising therapeutic agent for preventing and treating BPA-related liver diseases.
Citation - WoS: 1
Ameliorative Effects of Agomelatine Against Doxorubicin-Induced Hepatotoxicity
(BMC, 2025) Savas, Hasan Basri; Sozen, Mehmet Enes; Cuce, Gokhan; Batur, Tuba
Drug-induced hepatotoxicity is a significant impediment to the use of doxorubicin, a commonly employed chemotherapeutic agent with established efficacy in cancer treatment. The present study aimed to determine the potential protective effects of agomelatine against doxorubicin hepatotoxicity in rat toxicity models. Thirty-two rats were divided into four groups: control (with saline administration), Doxo (with 40 mg/kg doxorubicin administration), Doxo + Ago20, and Doxo + Ago40 (with 20 and 40 mg/kg agomelatine administration and 40 mg/kg doxorubicin administration). On the day of 14 rats were sacrificed, samples were collected for comparison of immunohistochemical, hematological, and biochemical analysis. There were statistically significant differences between the study groups in terms of immunohistochemical, hematological, and biochemical parameters. Agomelatine administration reduced the TNF-alpha, and caspase-3, which increased by doxorubicin, and reversed levels of oxidative stress markers altered by doxorubicin (p < 0.05). Doxorubicin induces oxidative stress, apoptosis, and hepatotoxicity. Agomelatine may be favored as a primary antidepressant to mitigate hepatic damage induced by doxorubicin.
Effects of Carvacrol on Oxidative Stress and Fibrosis in Streptozotocin-Induced Diabetic Nephropathy: Histological, Gene Expression, and Biochemical Insights
(MDPI, 2025) Canbaz, Halime Tuba; Sozen, Mehmet Enes; Cinar Ayan, Ilknur; Savas, Hasan Basri; Canbaz, Furkan Adem; Cuce, Gokhan; Kalkan, Serpil
Diabetes mellitus (DM) leads to renal damage through oxidative stress. Carvacrol (CAR), a monoterpenoid phenol, possesses anti-inflammatory and antioxidant properties. We investigated the potential effects of CAR on histological, gene expression, and biochemical parameters in a rat model of DM. Four groups were created: group 1, control; group 2 (n = 9), DM; group 3 (n = 9), DM + dimethyl sulfoxide (DMSO); and group 4 (n = 9), DM + CAR. DM was created by injecting streptozotocin (STZ). CAR (20 mg/kg) was prepared through dissolution in 0.1% DMSO. CAR and 0.1% DMSO were administered daily for 4 weeks to groups 4 and 3, respectively. At the end of this study, urea, creatinine, paraoxonase-1 (PON-1), and arylesterase (ARES) were measured in serum samples. Histopathological changes and expression of Nuclear factor erythroid 2-related factor 2 (Nrf-2) in renal tissues were assessed. Immunohistochemical(ihc) staining and RT-qPCR analysis were performed to evaluate apoptosis, focusing on Bax and Bcl-2gene expression. Masson's trichrome(MT) staining and RT-qPCR analysis of COL1A1 and COL3A1 mRNA levels were used to assess fibrosis. Increased urea and creatinine levels in DM were significantly decreased after CAR administration. CAR application also improved reduced levels of PON 1 and ARES, which are associated with diabetes. Both immunohistochemistry and RT-qPCR analyses revealed that CAR therapy mitigated the diabetes-induced elevation in Bax and reduction in Bcl-2 expression. CAR treatment improved histopathological findings and renal Nrf-2 immunofluorescence(if) intensity. Furthermore, gene expression analysis demonstrated that COL1A1 and COL3A1 were upregulated in DM, while CAR administration downregulated them. In conclusion, CAR has a protective role in decreasing renal impairment linked to DM by regulating Bax and Bcl-2 levels and rectifying histological damage.
Citation - WoS: 17
Citation - Scopus: 15
Protective Effects of Selenium Against Acrylamide-Induced Hepatotoxicity in Rats
(Univ Agriculture, Fac veterinary Science, 2024) Sozen, Mehmet Enes; Savas, Hasan Basri; Cuce, Gokhan
Acrylamide (ACR) is an organic chemical widely consumed worldwide, depending on the diet. ACR has toxic effects on the liver and other organs due to oxidative damage. The research is aimed to determine the effects of Selenium (Se) against ACR toxicity. 32 Wistar albino male rats were divided into Control, ACR, Se, and ACR+Se groups. After slaughter on the 28 th day, the blood samples taken from the animals were tested for total oxidant status (TOS) and total antioxidant status (TAS) to assess oxidative stress. The liver tissue sections were evaluated for lymphocyte infiltration, hepatocyte degeneration, sinusoid dilatation, and congestion. IL-6, Bax, and Bcl-2 expression were evaluated with immunohistochemistry. While the ACR group's TOS and oxidative stress index (OSI) values were significantly higher than the control group's, there was no significant difference in the ACR+Se group's TOS and OSI values. The ACR group had a considerably higher histopathological score than the other groups. ACR increased IL-6, and Bax levels and decreased Bcl-2 levels compared to the control, Se, and ACR+Se groups. ACR increased oxidative stress significantly caused toxic effects, inflammation, and cell death in the liver. On the other hand, Se oral supplementation may protect against oxidative stress, toxic effects, inflammation, and cell death induced by ACR in the liver.