Browsing by Author "Doğru, Mehmet"

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Antioxidant and Antimicrobial Capacity of Quinic Acid
(Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 2022) Ercan, Leyla; Doğru, Mehmet
Recently, agents with natural antioxidant and antimicrobial properties have been popularly studied. For this purpose, phenolic compounds, terpenes, and organic acids are examined for their antioxidant and antimicrobial properties. Of these, organic acids are increasingly being used in pharmacology, medicine, food, and industry. Quinic acid is a natural organic compound found in many edible fruits and plants. In this study, the antioxidant effect of quinic acid, which has the structure of cyclohexane carboxylic acid, was determined in vitro using seven different methods (DPPH, ABTS, CUPRAC, DMPD, FRAP, Fe3+ reduction, and Total antioxidant method). In addition, its antimicrobial effect on fungi (C. albicans), gram-positive bacteria (S. aureus, S. pyogenes), and gram-negative bacteria (E. coli, K. pneumoniae, and P. aeruginosa) were determined by the disk diffusion method. As a result, it was found that quinic acid has broad-spectrum antimicrobial properties, but its antioxidant properties are too low to be highlighted. While its antimicrobial activity was quite good, especially on K. pneumoniae E. coli, S. aureus, S. Pyogenes, and P. aeruginosa, it did not show any effect on C. albicans. Although the antioxidant property of quinic acid is low, it showed more antioxidant properties in the DMPD method, which is one of these methods, because it dissolves very well in water.
Determination of Phenolic Compounds in Nasturtium Officinale by Lc-Ms / Ms Using Different Extraction Methods and Different Solvents
(2023) Ercan, Leyla; Doğru, Mehmet
This study aimed to determine the phenolic compound contents of Nasturtium officinale (N. officinale) extracts prepared with water, methanol, ethanol, and chloroform, as well as extracts prepared by evaporation and lyophilization, and parts of N. officinale by LC-MS/MS (Liquid Chromatography-Mass spectrometry/Mass spectrometry). The results obtained not only contribute to knowledge about the benefits of N. officinale but also show the effect of different solvents and different extraction methods on the determination of phenolic compounds. In addition to these results, the importance of the cold chain in the determination of some phenolic compounds has also been demonstrated. While it is observed that the number of phenolic components obtained from the extracts prepared with methanol and ethanol is high; It is observed that some phenolic compounds such as quinic acid, fumaric acid, aconitic acid, and p-coumaric acid dissolve very well in water and temperature is important.