Browsing by Author "Firat, Fatih"

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Citation - WoS: 6
Citation - Scopus: 5
The protective effects of sinapic acid on acute renal ischemia/reperfusion injury
(Turkish Journal of Biochemistry, 2021) Unsal, Velid; kolukcu, Engin; Firat, Fatih; Gevrek, Fikret
Objectives: The aim of this research was to investigate whether sinapic acid (SA) can alleviate oxidative damage, apoptosis, and inflammation in I/R induced renal injury. Methods: A total of 24 male rats were randomly separated into four groups as six rats in each group. Group 1 (Sham), Group 2 (I/R), Group 3 (I/R + SA, 10mg/kg), Group 4 (I/R + SA, 20 mg/kg). In order to evaluate kidney function serum BUN, Cr, and AST were measured in an autoanalyzer. SOD, GSH-Px, MDA, PC and NO oxidative stress parameters were measured with spectrophotometric methods and TNF-α, IL-1β, IL-6, KIM-1 and NGAL parameters were measured with the ELISA method. In addition, H&E method and immunohistochemical examinations were performed for histological evaluations of kidney tissue. Results: SA significantly decreases the increase in kidney damage, inflammation, oxidative stress, cell death and restore the decrease in antioxidant enzyme activities (p<0.05). Pre-treatment of the rats with SA reduces kidney dysfunction and morphological changes. Conclusions: The development of oxidative stress and lipid peroxidation seems to be the leading factors that accelerate inflammation and cell death during renal IRI. The antioxidant, anti-inflammatory, and anti-apoptotic features of SA displayed a renoprotective effect.
The Potential Renoprotective Effect of Sugammadex in Renal Ischemia-Reperfusion Injury
(Galenos Publ House, 2025) Kolukcu, Vildan; Bala, Mehtap Gurler; Sahin, Ahmet Tugrul; Genc, Ali; Unsal, Velid; Firat, Fatih; Gurpinar, Ahmer Burak
Objective: We aimed to evaluate the effectiveness of sugammadex on renal tissue for against ischemia-reperfusion injury. Methods: Twenty-one Wistar albino strain female rats were divided into three groups. The first group functioned as the control cohort for comparison. In Groups 2 and 3, a renal ischemia-reperfusion model was established. Moreover, following the cessation of ischemia, the rats in Group 3 were intravenously administered sugammadex at a dose of 4 mg kg-1. Blood and tissue samples were subsequently collected for analysis. Results: Biochemical analyses revealed a notable increase in the enzymatic activities of glutathione peroxidase and superoxide dismutase in Group 3 relative to Group 2 (P < 0.001 and P=0.015, respectively). Additionally, the concentration of malondialdehyde was found to be significantly reduced in Group 3 relative to Group 2 (P=0.004). Group 3 exhibited a substantial decrease in tumor necrosis factor-alpha, interleukin 6, and interleukin 1 beta levels when compared to Group 2 (P=0.021, P=0.006, and P=0.016 respectively). Group 2 exhibited the highest concentrations of neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 (P < 0.001 and P=0.015, respectively). Similarly, the histopathologic tissue damage was the most prominent in Group 2 (P < 0.001). Conclusion: Sugammadex plays a protective role against ischaemia-reperfusion injury in renal tissue.