Browsing by Author "Firat, Mehmet"

Now showing 1 - 3 of 3

Determination and comparison of metal contents in simulated body fluid medium conditions of the plant species by flame atomic absorption spectrometry (FAAS)
(Journal of Research in Pharmacy, 2022) Umaz, Adil; Firat, Aydin; Tokul Olmez, Ozge; Firat, Mehmet; Ertas, Abdulselame; Aydin, Isil
ABSTRACT: The importance of plants in folk medicine and scientific studies is increasing day by day. In this context, the plants pose a danger to human health when they are eaten by the public, consumed as herbal tea or used as medicinal plants especially the toxic metals in their composition. For this reason, determining the macro, micro and toxic element content in the plants is important for health. In this study; the metal contents of root and aerial parts of nine different Salvia species were determined and the chemometric evaluation of the obtained results was made. In the principal component analysis (PCA) made with 20 elements of Salvia species, it was determined that the first two principal components explained 62.60% of the variance and the first six principal components explained 92.70%. When PCA and hierarchical cluster analysis (HCA) results are evaluated; the parts of Salvia species root and aerial parts were determined that were not clearly separated and there was no regional grouping. In addition, Salvia species prepared as herbal tea was left to different simulated body fluid medium conditions, and the changes in metal contents were examined. It was determined that were taken up Cr, Cd, Pb, Cu, Zn metals in the simulated saliva fluid (SSF), Ni, Mn metals in the simulated gastric fluid (SGF), and Na, K metals in the simulated intestinal fluid (SIF). It was determined that Fe metal in almost all Salvia species was not taken from three simulated body fluid mediums. Thus, elements determined which were taken up in body fluid mediums.
An Investigation and Comparison of Concentration Change in Simulated Body Fluid Medium Conditions of the Calcium Element in 27 Different Salvia Species
(Istanbul Univ, Fac Pharmacy, 2023) Umaz, Adil; Aydin, Firat; Firat, Mehmet; Yener, Ismail; Ertas, Abdulselam; Elmastas, Ayhan
Background and Aims: In this study, calcium (Ca) element concentration changes of Salvia species in simulated body fluid (SBF) medium conditions were investigated and the results in these medium conditions were compared with each other.Methods: Salvia species samples were air-dried and ground into powder. Salvia species was prepared as a herbal tea. Pre-pared teas were left in three different SBF medium conditions. The samples were analyzed using Flame Atomic Absorption Spectroscopy (FAAS) method to determine the Ca absorptions. Also, a correlation analysis of the results obtained in three different SBF medium conditions and the species in simulated gastric fluid (SGF) medium conditions was performed.Results: When the SBF medium conditions were compared, it was determined that the highest Ca absorption of all Salvia species occurred in the SGF conditions. Thus, it can be said that the Ca in plants and foods occurs in the gastric fluid medium and its absorption occurs there. When the result of the correlation analysis was evaluated, it was determined that there was a stronger correlation between the SGF and simulated intestinal fluid (SIF) mediums compared to other mediums.Conclusion: The Ca absorption was determined according to what remained in the SGF the most. Based on the results ob-tained from SBF medium conditions, it can be said which element is taken in which body fluid medium. Information on the differences between samples belonging to different SBF medium conditions was not obtained. However, when the simulated fluid medium conditions were evaluated individually, information was obtained for two or more samples.
Lc-ms/Ms Analysis and Biological Activities of Different Parts Of Ziziphora Capitata L.
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Çavuşoğlu, Mehmet; Cavusoglu, Mehmet; Caglayan, Mehmet Veysi; Yener, Ismail; Firat, Mehmet; Cavus Kaya, Eda; Ertas, Abdulselam
The Ziziphora species, classified under the Lamiaceae family, have a strong aromatic property. Ziziphora species have been used in folk medicine as sedative, gastric, aphrodisiac, bloating, and degassing. In the current study, the phenolic and flavanoid content of ethanol extracts of Ziziphora capitata L. species of flower, leaf, branch, mixed, and root parts was determined by the LC-MS/MS device. In addition, the antioxidant and cytotoxic activities of the extracts, as well as their inhibitory effects on enzymes (antihypertensive, AchE (acetylcholinesterase), BchE ( butyrylcholinesterase), elastase, tyrosinase, collagenase and urease), were determined. The LC-MS/MS results showed that quinic acid (25578, 5842, 25171, 14055, 10597 mu g g-1, respectively) was found in higher amounts in flower, leaf, branch, mixed, and root extracts of Z. capitata species compared to other components. Additionally, rosmarinic acid (17097 mu g g-1), cynaroside (8432), and hesperidin (8067) were found to be major components. It was observed that the flower extract of the species exhibited strong antioxidant activity (IC50: 37.18 +/- 1.36 mu g mL-1, 9.89 +/- 0.45, A0.5:16.27 +/- 0.02, respectively) in DPPH, ABTS and CUPRAC methods. It was concluded that the leaf extract of Z. capitata species had a strong cytotoxic effect on HT-29 (colon cancer cell line) (viability %: 9.26 +/- 0.69). It was observed that the root part of the species exhibited higher activity in butyrylcholinesterase (BChE) enzyme inhibition activity (inhibition %: 40.56 +/- 0.88) than other parts. It was determined that Z. capitata extracts did not show acetylcholinesterase, urease, tyrosinase, elastase, collagenase, and antihypertensive enzyme activity or showed low activity. As a result, it is thought that the flower extract of the Z. capitata species has better results in terms of the examined parameters, whereas the leaf extract needs to be subjected to more detailed in vitro and in vivo research conducted to be used in the pharmaceutical industry as a result of its cytotoxic effect against colon cancer cell lines.