Browsing by Author "Gevrek, Fikret"

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An Animal Model of Reperfusion in Ischemic Corporal Tissue and the Effect of Sugammadex on Oxidative Injury Parameters
(MRE Press, 2025) Kolukcu, Vildan; Yalcin, Kenan; Eroglu, Askin; Gevrek, Fikret; Gurpinar, Ahmet Burak; Unsal, Velid
Background: The aim of this study was to analyze the effect of sugammadex on ischemia-reperfusion injury in corporal tissue. Methods: Eighteen male Wistar Albino strain rats were divided into three groups. Group 1 served as the control group. A priapism model was induced in rats in Group 2 and 3. Rats in Group 3 were additionally administered 4 mg/kg sugammadex intravenously immediately after reperfusion. All rats underwent penectomy for histopathological and biochemical evaluations, and blood samples were collected. Results: In Group 2, total oxidant status (TOS) and malondialdehyde (MDA) levels were significantly higher compared to other groups (p < 0.001 and p = 0.009, respectively). A substantial reduction in both TOS and MDA levels was found in Group 3 (p <0.001 and p = 0.043, respectively). Group 2 exhibited significantly lower activities of a glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) compared to Group 1 (p = 0.043 and p = 0.024, respectively). In contrast, Group 3 showed significant increases in both antioxidant enzyme activities (p = 0.036 and p = 0.034, respectively). Similarly, the total antioxidant status (TAS) was dramatically higher in Group 3 compared to Group 2 (p < 0.001). Microscopic examinations revealed improvements in vasocongestion, edema, desquamation and inflammation scores in Group 3 compared to Group 2 (p < 0.001). Conclusions: Biochemical and histopathological findings suggest that, sugammadex significantly mitigates oxidative damage parameters in corporal tissue affected by ischemia-reperfusion injury.
Citation - WoS: 2
Citation - Scopus: 1
Effects of Ketamine on Penile Tissues in an Experimental Priapism Model in Rats
(Turkish Assoc Trauma Emergency Surgery, 2024) Gevrek, Fikret; Fırat, Fatih; Unsal, Velid; Tapar, Hakan; Karaman, Tugba; Karaman, Serkan; Yalçın, Kenan
BACKGROUND: This study aimed to evaluate the histopathological and biochemical effects of ketamine on penile tissues following ischemia-reperfusion injury induced by priapism. METHODS: Twenty-four male rats were randomized into three groups. Group 1 served as the control group. Group 2 underwent the priapism model to induce ischemia-reperfusion injury. Group 3, the treatment group, experienced a similar ischemia-reperfusion model as Group 2; additionally, 50 mg/kg of ketamine was administered intraperitoneally just before reperfusion. Blood biochemical analyses and penile histopathological evaluations were performed. RESULTS: In Group 3, significant improvements were observed in all histopathological scores, including desquamation, edema, inflammation, and vasocongestion compared to Group 2 (p<0.001). Blood biochemical analyses showed that the malondialdehyde (MDA) levels were recorded as 10 in Group 2, with a significant decrease in Group 3 (p=0.013). Similarly, proinflammatory cytokine levels, including interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), were found to be suppressed in Group 3 compared to Group 2 (p=0.003, p=0.022, and p=0.028, respectively). Antioxidant enzyme activities, such as glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), were higher in Group 3 compared to Group 2 (p=0.016 and p=0.024, respec- tively). CONCLUSION: Ketamine is an effective anesthetic agent in alleviating the effects of penile ischemia-reperfusion injury.
Citation - WoS: 6
Citation - Scopus: 6
Etomidate Alleviates Ovarian Ischemia-Reperfusion Injury in Rats
(Turkish Assoc Trauma Emergency Surgery, 2024) Katar, Muzaffer; Gevrek, Fikret; Unsal, Velid; Tapar, Hakan; Karaman, Tugba; Karaman, Serkan; Balta, Mehtap
BACKGROUND: This study investigates the protective effects of etomidate against oxidative damage in an experimental model of ovarian ischemia-reperfusion injury. METHODS: A total of 24 female rats were randomized into three groups. Group 1 served as the control. Group 2 underwent an ovarian torsion/detorsion procedure. Group 3 underwent similar procedures as Group 2; additionally, 4 mg/kg of etomidate was administered intraperitoneally 30 minutes before ovarian detorsion. Blood samples were analyzed for lipid peroxidation, pro-inflamma- tory cytokine levels, and antioxidant enzyme activity.Furthermore, histopathological scoring was performed to evaluate tissue damage in the ovaries. RESULTS: Biochemical analysis of blood samples revealed reductions in pro-inflammatory cytokines, including interleukin-1 Beta (IL- 1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), in Group 3 compared to Group 2 (p=0.005, p=0.016, and p<0.001, respectively). Additionally, a decrease in malondialdehyde (MDA) levels was observed in Group 3 compared to Group 2 (p<0.001). In contrast, activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), were signifi- cantly increased in Group 3 compared to Group 2 (p=0.031 and p=0.001, respectively). Furthermore, Group 3 demonstrated notable reductions in histopathological scores for follicular degeneration, vascular occlusion, bleeding, and inflammation compared to Group 2 (p<0.001, p<0.001, p<0.001, and p=0.001, respectively). CONCLUSION: Etomidate alleviates ischemia-reperfusion injury in a rat ovarian torsion-detorsion model by improving both histo- pathological and biochemical outcomes.
Citation - WoS: 1
Citation - Scopus: 1
Is There Any Effect of Lidocaine on Ischemia/Reperfusion Injury in Testicular Torsion? An Experimental Study
(Turkish Assoc Trauma Emergency Surgery, 2024) Gevrek, Fikret; Fırat, Fatih; Unsal, Velıd; Kılınç, Fatih; Tapar, Hakan; Karaman, Serkan; Yalçın, Kenan
BACKGROUND: This experimental study aimed to evaluate the potential protective effects of lidocaine on ischemia-reperfusion injury resulting from testicular torsion/detorsion in rats. METHODS: A total of 18 male rats were randomized into three groups. Group 1 served as the control group. Group 2 was designed to evaluate testicular ischemia-reperfusion injury using a torsion/detorsion model. In Group 3, the treatment group, a similar ischemia- reperfusion model was used as in Group 2. Additionally, lidocaine at a dose of 15 mg/kg was administered intraperitoneally five minutes before reperfusion. Blood biochemical analyses and testicular histopathological evaluations were conducted. RESULTS: Blood biochemical analysis showed that malondialdehyde (MDA) and protein carbonyl (PC) levels were significantly higher in Group 2 compared to the other groups (p<0.001 and p=0.008, respectively). Proinflammatory cytokine levels, including interleu- kin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha), were lower in Group 3 than in Group 2 (p<0.001, p=0.007, and p=0.026, respectively). Antioxidant enzyme activities, including glutathione peroxidase (GSH-Px) and superoxide dis- mutase (SOD), were higher in Group 3 compared to Group 2 (p=0.005 and p=0.025, respectively). Histopathological evaluations revealed significant improvements in all testicular damage scores, including hemorrhage, edema, vasocongestion, and inflammation in Group 3 compared to Group 2 (p=0.015, p=0.035, p=0.015, and p=0.034, respectively). Additionally, there was a notable improvement in the Johnsen score in Group 3 compared to Group 2 (p=0.034). CONCLUSION: Lidocaine, an effective local anesthetic, significantly alleviates the effects of testicular ischemia-reperfusion injury.
Citation - WoS: 1
Citation - Scopus: 1
Possible Protective Effect of Remifentanil Against Testicular Ischemia-Reperfusion Injury
(Walter de Gruyter GmbH, 2024) Katar, Muzaffer; Gevrek, Fikret; Fırat, Fatih; Unsal, Velıd; Tapar, Hakan; Karaman, Tugba; Kuyucu, Yunus Emre
Objectives: This study aims to evaluate the protective effi- cacy of remifentanil against testicular ischemia-reperfusion injury. Methods: The study included 24 male rats. The rats were randomized into three groups: Group 1 was the control group. Group 2 was subjected to a testicular torsion/ detorsion model. Group 3 underwent similar procedures and additionally received remifentanil (0.6 μg/kg/min) intrave- nously for the first 20 min of reperfusion. Blood samples were taken for biochemical analyses, and orchiectomy was performed for histopathologic examination. Results: Biochemical analysis of blood samples showed a significant increase in antioxidant enzyme activity, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in Group 3 compared to Group 2 (p:0.004 and p:0.002, respectively). There was a dramatic decrease in the levels of proinflammatory cytokines, including interleukin-1 beta (IL-1 Beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) in Group 3 compared to Group 2 (p:0.001, p:0.046, and p:0.004, respectively). Similarly, malondialdehyde (MDA) levels decreased in Group 3 compared to Group 2 (p:0.004). Histopathologic examination of Group 3 rats showed positive changes in inflammation, hemorrhage, edema, and conges- tion levels compared to Group 2 (p<0.001). Similarly, there was a positive effect on the Johnsen and Cosentino score in Group 3 compared to Group 2 (p:0.001 and p<0.001, respectively). Conclusions: In our study, it has been documented that remifentanil protects against testicular ischemia-reperfusion injury.
The Potential Renoprotective Effect of Sugammadex in Renal Ischemia-Reperfusion Injury
(Galenos Publishing House, 2025) Gevrek, Fikret; Fırat, Fatih; Unsal, Velid; Kolukcu, Vildan; Sahin, Ahmet Tugrul; Balta, Mehtap; Yancı, Asiye
Objective: We aimed to evaluate the effectiveness of sugammadex on renal tissue for against ischemia-reperfusion injury. Methods: Twenty-one Wistar albino strain female rats were divided into three groups. The first group functioned as the control cohort for comparison. In Groups 2 and 3, a renal ischemia-reperfusion model was established. Moreover, following the cessation of ischemia, the rats in Group 3 were intravenously administered sugammadex at a dose of 4 mg kg-1. Blood and tissue samples were subsequently collected for analysis. Results: Biochemical analyses revealed a notable increase in the enzymatic activities of glutathione peroxidase and superoxide dismutase in Group 3 relative to Group 2 (P < 0.001 and P=0.015, respectively). Additionally, the concentration of malondialdehyde was found to be significantly reduced in Group 3 relative to Group 2 (P=0.004). Group 3 exhibited a substantial decrease in tumor necrosis factor-alpha, interleukin 6, and interleukin 1 beta levels when compared to Group 2 (P=0.021, P=0.006, and P=0.016 respectively). Group 2 exhibited the highest concentrations of neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 (P < 0.001 and P=0.015, respectively). Similarly, the histopathologic tissue damage was the most prominent in Group 2 (P < 0.001). Conclusion: Sugammadex plays a protective role against ischaemia-reperfusion injury in renal tissue.
Citation - WoS: 6
Citation - Scopus: 5
The protective effects of sinapic acid on acute renal ischemia/reperfusion injury
(Turkish Journal of Biochemistry, 2021) Unsal, Velid; kolukcu, Engin; Firat, Fatih; Gevrek, Fikret
Objectives: The aim of this research was to investigate whether sinapic acid (SA) can alleviate oxidative damage, apoptosis, and inflammation in I/R induced renal injury. Methods: A total of 24 male rats were randomly separated into four groups as six rats in each group. Group 1 (Sham), Group 2 (I/R), Group 3 (I/R + SA, 10mg/kg), Group 4 (I/R + SA, 20 mg/kg). In order to evaluate kidney function serum BUN, Cr, and AST were measured in an autoanalyzer. SOD, GSH-Px, MDA, PC and NO oxidative stress parameters were measured with spectrophotometric methods and TNF-α, IL-1β, IL-6, KIM-1 and NGAL parameters were measured with the ELISA method. In addition, H&E method and immunohistochemical examinations were performed for histological evaluations of kidney tissue. Results: SA significantly decreases the increase in kidney damage, inflammation, oxidative stress, cell death and restore the decrease in antioxidant enzyme activities (p<0.05). Pre-treatment of the rats with SA reduces kidney dysfunction and morphological changes. Conclusions: The development of oxidative stress and lipid peroxidation seems to be the leading factors that accelerate inflammation and cell death during renal IRI. The antioxidant, anti-inflammatory, and anti-apoptotic features of SA displayed a renoprotective effect.
Citation - WoS: 1
Renoprotective effect of diacerein in rats with partial unilateral ureteral obstruction model
(Walter de Gruyter, 2024) Katar, Muzaffer; Gevrek, Fikret; Fırat, Fatih; Kölükçü, Engin; Unsal, Velıd
Objectives: We aimed to analyze the effects of diacerein in a rat model of partial unilateral ureteral obstruction (PUUO). Methods: We randomly divided 24 female rats into three groups. Control group, PUUO group and PUUO + diacerein group. The PUUO group was subjected to the PUUO model for seven days. The PUUO + diacerein group received oral diacerein (80 mg/kg) for seven days. Spectrophotometric methods were employed to measure oxidative stress parameters, including malondialdehyde (MDA), protein carbonyl (PC) and antioxidant enzyme levels, including glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD), while indicators of renal function, such as kidney injury molecule-1 (KIM-1) and neutrophil gelatinous- associated lipocalin (NGAL), along with inflammatory parameters interleukin-1 beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6), were assessed using the ELISA method. Inflammatory parameters were measured in blood samples, and other parameters were analyzed in kidney tissue. Hematoxylin-eosin method examinations were used for histological analyses. Results: IL-1beta, TNF-alpha, and IL-6 levels were found to be significantly decreased in the PUUO + diacerein group compared to the PUUO group (p=0.006, p=0.002 and p=0.001, respectively). In the PUUO + diacerein group, GSH-Px and SOD activities increased compared with the PUUO group (p=0.031 and p=0.037, respectively). We also observed a significant improvement in renal function parameters, such as KIM-1 and NGAL levels in the PUUO + diacerein group compared to PUUO (p=0.002 and p=0.012, respectively). The PC and MDA levels were highest in the PUUO group (p<0.001). Similarly, the histopathologic tissue damage was the most prominent PUUO group (p<0.001). Conclusions: Our study found that diacerein is a highly effective pharmacologic agent in alleviating oxidative dam- age in PUUO model rats.
Citation - WoS: 25
Citation - Scopus: 27
Sinapic acid reduces ischemia/reperfusion injury due to testicular torsion/detorsion in rats
(Andrologia, 2021) Unsal, Velid; Kölükçü, Engin; Gevrek, Fikret; Fırat, Fatih
This study aimed to investigate the protective effect of sinapic acid (SA) on biochemical and histopathological changes in an experimental testicular torsion-detorsion rat model. Twenty-four rats were randomised into four groups: sham group, ischemia/reperfusion (IR) group subjected to testicular torsion for 2 hr and then detorsion for 4 hr, and two groups treated with SA1 and SA2 (10 mg/kg and 20 mg/kg, by single intraperitoneal injection, 30 min before reperfusion). Serum testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were measured by an autoanalyzer, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), protein carbonyl (PC), and nitric oxide (NO) oxidative stress parameters by spectrophotometric methods, and tumour necrosis factor (TNF-α), interleukin-1 beta (IL-1β), and interleukin 6 (IL-6) parameters by the Elisa method. In addition, immunohistochemical and histopathological examinations were performed on testicular tissues. There was no significant difference between the groups in terms of serum testosterone, FSH and LH levels (p >.05). SA significantly reduced increased testicular damage, oxidative stress, inflammation, cell death and also restored decreased antioxidant enzyme activities (p <.05). Pre-treatment of rats with SA reduced testicular dysfunction and morphological changes IRI. SA's antioxidant, anti-inflammatory, and antiapoptotic properties were found to be protective against testicular IR
Sinapik Asidin Akut Renal İskemi/reperfüzyon Hasarı Üzerine Koruyucu Etkileri
(2021) Unsal, Velid; Kolukcu, Engin; Fırat, Fatih; Gevrek, Fikret
Giriş: Bu araştırmanın amacı, I/R kaynaklı böbrek hasa- rında sinapik asidin (SA) oksidatif hasarı, apoptozu ve inflamasyonu hafifletip hafifletemeyeceğini araştırmaktır. Yöntemler: Toplam 24 erkek sıçan her grupta 6 adet sıçan olacak şekilde rastgele 4 gruba ayrıldı. Grup 1 (Sham), Grup 2 (I/R), Grup 3 (I/R + SA, 10 mg/kg), Grup 4 (I/R + SA, 20 mg/kg). Böbrek fonksiyonunu değerlendirmek için serum BUN, Cr ve AST otoanalizörde ölçüldü. SOD, GSH-Px, MDA, PC ve NO oksidatif stres parametreleri spektrofotometrik yöntemlerle, TNF-α, IL-1β, IL-6, KIM-1 ve NGAL parametreleri ELISA yöntemiyle ölçüldü. Ayrıca böbrek dokusunun histolojik değerlendirmeleri için H&E yöntemi ve immünohistokimyasal incelemeler yapıldı. Bulgular: SA, böbrek hasarı, inflamasyon, oksidatif stres, hücre ölümündeki artışı önemli ölçüde azaltır ve antiok- sidan enzim aktivitelerindeki azalmayı geri kazandırır (p<0.05). Sıçanların SA ile ön tedavisi böbrek fonksiyon bozukluğunu ve morfolojik değişiklikleri azaltır. Sonuç: Oksidatif stres ve lipid peroksidasyonunun geli- şimi, renal IRI sırasında inflamasyonu ve hücre ölümünü hızlandıran önde gelen faktörler gibi görünmektedir. SA’nın antioksidan, anti-inflamatuar ve anti-apoptotik özellikleri, renoprotektif bir etki göstermiştir.