Browsing by Author "Gevrek, Fikret"

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Effects of ketamine on penile tissues in an experimental priapism model in rats
(Ali Cangül, 2024) Kölükçü, Vildan; Gürler Balta, Mehtap; Tapar, Hakan; Karaman, Tuğba; Karaman, Serkan; Ünsal, Velid; Gevrek, Fikret; Yalçın, Kenan; Fırat, Fatih
Background: This study aimed to evaluate the histopathological and biochemical effects of ketamine on penile tissues following ischemia-reperfusion injury induced by priapism. Methods: Twenty-four male rats were randomized into three groups. Group 1 served as the control group. Group 2 underwent the priapism model to induce ischemia-reperfusion injury. Group 3, the treatment group, experienced a similar ischemia-reperfusion model as Group 2; additionally, 50 mg/kg of ketamine was administered intraperitoneally just before reperfusion. Blood biochemical analyses and penile histopathological evaluations were performed. Results: In Group 3, significant improvements were observed in all histopathological scores, including desquamation, edema, inflammation, and vasocongestion compared to Group 2 (p<0.001). Blood biochemical analyses showed that the malondialdehyde (MDA) levels were recorded as 10 in Group 2, with a significant decrease in Group 3 (p=0.013). Similarly, proinflammatory cytokine levels, including interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), were found to be suppressed in Group 3 compared to Group 2 (p=0.003, p=0.022, and p=0.028, respectively). Antioxidant enzyme activities, such as glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), were higher in Group 3 compared to Group 2 (p=0.016 and p=0.024, respec-tively). Conclusion: Ketamine is an effective anesthetic agent in alleviating the effects of penile ischemia-reperfusion injury.
The protective effects of sinapic acid on acute renal ischemia/reperfusion injury
(Turkish Journal of Biochemistry, 2021) Unsal, Velid; kolukcu, Engin; Firat, Fatih; Gevrek, Fikret
Objectives: The aim of this research was to investigate whether sinapic acid (SA) can alleviate oxidative damage, apoptosis, and inflammation in I/R induced renal injury. Methods: A total of 24 male rats were randomly separated into four groups as six rats in each group. Group 1 (Sham), Group 2 (I/R), Group 3 (I/R + SA, 10mg/kg), Group 4 (I/R + SA, 20 mg/kg). In order to evaluate kidney function serum BUN, Cr, and AST were measured in an autoanalyzer. SOD, GSH-Px, MDA, PC and NO oxidative stress parameters were measured with spectrophotometric methods and TNF-α, IL-1β, IL-6, KIM-1 and NGAL parameters were measured with the ELISA method. In addition, H&E method and immunohistochemical examinations were performed for histological evaluations of kidney tissue. Results: SA significantly decreases the increase in kidney damage, inflammation, oxidative stress, cell death and restore the decrease in antioxidant enzyme activities (p<0.05). Pre-treatment of the rats with SA reduces kidney dysfunction and morphological changes. Conclusions: The development of oxidative stress and lipid peroxidation seems to be the leading factors that accelerate inflammation and cell death during renal IRI. The antioxidant, anti-inflammatory, and anti-apoptotic features of SA displayed a renoprotective effect.
Renoprotective effect of diacerein in rats with partial unilateral ureteral obstruction model
(WALTER DE GRUYTER, 2024) Kölükçü, Engin; Unsal, Velid; Fırat, Fatih; Gevrek, Fikret; Katar, Muzaffer
Objectives: We aimed to analyze the effects of diacerein in a rat model of partial unilateral ureteral obstruction (PUUO). Methods: We randomly divided 24 female rats into three groups. Control group, PUUO group and PUUO + diacerein group. The PUUO group was subjected to the PUUO model for seven days. The PUUO + diacerein group received oral diacerein (80 mg/kg) for seven days. Spectrophotometric methods were employed to measure oxidative stress parameters, including malondialdehyde (MDA), protein carbonyl (PC) and antioxidant enzyme levels, including glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD), while indicators of renal function, such as kidney injury molecule-1 (KIM-1) and neutrophil gelatinous-associated lipocalin (NGAL), along with inflammatory parameters interleukin-1 beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6), were assessed using the ELISA method. Inflammatory parameters were measured in blood samples, and other parameters were analyzed in kidney tissue. Hematoxylin-eosin method examinations were used for histological analyses. Results: IL-1beta, TNF-alpha, and IL-6 levels were found to be significantly decreased in the PUUO + diacerein group compared to the PUUO group (p=0.006, p=0.002 and p=0.001, respectively). In the PUUO + diacerein group, GSH-Px and SOD activities increased compared with the PUUO group (p=0.031 and p=0.037, respectively). We also observed a significant improvement in renal function parameters, such as KIM-1 and NGAL levels in the PUUO + diacerein group compared to PUUO (p=0.002 and p=0.012, respectively). The PC and MDA levels were highest in the PUUO group (p<0.001). Similarly, the histopathologic tissue damage was the most prominent PUUO group (p<0.001). Conclusions: Our study found that diacerein is a highly effective pharmacologic agent in alleviating oxidative damage in PUUO model rats.
Sinapic acid reduces ischemia/reperfusion injury due to testicular torsion/detorsion in rats
(Andrologia, 2021) Unsal, Velid; Kölükçü, Engin; Gevrek, Fikret; Fırat, Fatih
This study aimed to investigate the protective effect of sinapic acid (SA) on biochemical and histopathological changes in an experimental testicular torsion-detorsion rat model. Twenty-four rats were randomised into four groups: sham group, ischemia/reperfusion (IR) group subjected to testicular torsion for 2 hr and then detorsion for 4 hr, and two groups treated with SA1 and SA2 (10 mg/kg and 20 mg/kg, by single intraperitoneal injection, 30 min before reperfusion). Serum testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were measured by an autoanalyzer, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), protein carbonyl (PC), and nitric oxide (NO) oxidative stress parameters by spectrophotometric methods, and tumour necrosis factor (TNF-α), interleukin-1 beta (IL-1β), and interleukin 6 (IL-6) parameters by the Elisa method. In addition, immunohistochemical and histopathological examinations were performed on testicular tissues. There was no significant difference between the groups in terms of serum testosterone, FSH and LH levels (p >.05). SA significantly reduced increased testicular damage, oxidative stress, inflammation, cell death and also restored decreased antioxidant enzyme activities (p <.05). Pre-treatment of rats with SA reduced testicular dysfunction and morphological changes IRI. SA's antioxidant, anti-inflammatory, and antiapoptotic properties were found to be protective against testicular IR
Sinapik Asidin Akut Renal İskemi/reperfüzyon Hasarı Üzerine Koruyucu Etkileri
(2021) Unsal, Velid; Kolukcu, Engin; Fırat, Fatih; Gevrek, Fikret
Giriş: Bu araştırmanın amacı, I/R kaynaklı böbrek hasa- rında sinapik asidin (SA) oksidatif hasarı, apoptozu ve inflamasyonu hafifletip hafifletemeyeceğini araştırmaktır. Yöntemler: Toplam 24 erkek sıçan her grupta 6 adet sıçan olacak şekilde rastgele 4 gruba ayrıldı. Grup 1 (Sham), Grup 2 (I/R), Grup 3 (I/R + SA, 10 mg/kg), Grup 4 (I/R + SA, 20 mg/kg). Böbrek fonksiyonunu değerlendirmek için serum BUN, Cr ve AST otoanalizörde ölçüldü. SOD, GSH-Px, MDA, PC ve NO oksidatif stres parametreleri spektrofotometrik yöntemlerle, TNF-α, IL-1β, IL-6, KIM-1 ve NGAL parametreleri ELISA yöntemiyle ölçüldü. Ayrıca böbrek dokusunun histolojik değerlendirmeleri için H&E yöntemi ve immünohistokimyasal incelemeler yapıldı. Bulgular: SA, böbrek hasarı, inflamasyon, oksidatif stres, hücre ölümündeki artışı önemli ölçüde azaltır ve antiok- sidan enzim aktivitelerindeki azalmayı geri kazandırır (p<0.05). Sıçanların SA ile ön tedavisi böbrek fonksiyon bozukluğunu ve morfolojik değişiklikleri azaltır. Sonuç: Oksidatif stres ve lipid peroksidasyonunun geli- şimi, renal IRI sırasında inflamasyonu ve hücre ölümünü hızlandıran önde gelen faktörler gibi görünmektedir. SA’nın antioksidan, anti-inflamatuar ve anti-apoptotik özellikleri, renoprotektif bir etki göstermiştir.