Browsing by Author "Guney, Inci Guler"

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First Report Of Colletotrichum Perseae Causing Anthracnose Disease of Avocado ( Persea Americana) in Türkiye
(Academic Press Ltd- Elsevier Science Ltd, 2024) Bozoglu, Tugba; Turkkan, Muharrem; Ozer, Goksel; Guney, Inci Guler; Dervis, Sibel
Colletotrichum-induced anthracnose is a major disease of avocado (Persea americana), causing significant losses in production worldwide. In 2023, a survey of avocado fruits in markets within the K & imath;z & imath;ltepe district of Mardin, T & uuml;rkiye, revealed symptoms of anthracnose, characterized by brown-black lesions and internal necrosis. To identify the causal agent, 20 symptomatic avocado fruits were collected and fungal isolates resembling Colletotrichum species were obtained. Morphological characterization, multilocus sequence analysis of the internal transcribed spacer (ITS), actin (act), the apn2-Mat1-2 intergenic spacer (ApMat), and calmodulin (cal) loci, and pathogenicity testing were performed for only two representative isolates (Pa01 and Pa02). These analyses identified the pathogen as Colletotrichum perseae. The isolates readily formed ascomata on all culture media tested, suggesting a high capacity for sexual reproduction. Pathogenicity tests confirmed that C. perseae caused characteristic dark, sunken anthracnose lesions on avocado fruits. This is the first report of C. perseae causing anthracnose in avocados in T & uuml;rkiye, expanding the known geographical distribution of this pathogen. The findings highlight the need for effective disease management strategies and further research to understand the pathogen's lifecycle, environmental conditions favoring infection, and potential for seedborne transmission.
First report of Neoscytalidium novaehollandiae on common sage (Salvia officinalis)
(Springer link, 2021) Dervis, Sibel; Guney, Inci Guler; Kosar, Islim; Bozoglu, Tugba; Ozer, Goksel
In June 2020, many plants exhibited symptoms of root rot and foliar blight in the experimental field of common sage in Sanliurfa province, Turkey. The pathogen was identified as Neoscytalidium novaehollandiae based on morphological characteristics and phylogenetic analysis of partial sequence of the transcription elongation factor 1-alpha gene and the internal transcribed spacer of rDNA. Koch's postulates were fulfilled by successful re-isolation of the pathogen from inoculated plants in the pathogenicity assay. To our best knowledge, this is the first report of N. novaehollandiae causing root rot and foliar blight of common sage worldwide.
Green Synthesis of pleurotus Eryngii-derived Nanomaterials for Phytopathogen Control
(Wiley-v C H verlag Gmbh, 2024) Acay, Hilal; Guney, Inci Guler; Yildirim, Ayfer; Dervis, Sibel; Dereli, Elif
Growing concerns over the human health and environmental impacts of conventional fungicides, coupled with the escalating challenge of microbial resistance, have fueled the search for sustainable biocontrol strategies against plant pathogens. This study reports, for the first time, the green synthesis and characterization of a novel, eco-friendly nanomaterial, designated Pleurotus eryngii-Lecithin-Chitosan Nanomaterial (PEELCN), derived from P. eryngii extract (PEE), lecithin (L), and chitosan (C). The structural attributes of PEELCN were elucidated using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA), X-ray Diffraction (XRD), and zeta potential measurements, confirming the successful formation of a stable and uniform nanostructure. The antifungal activity of PEELCN, and PEE, was assessed against five economically important phytopathogenic fungi: Neoscytalidium dimidiatum, Alternaria alternata, Verticillium dahliae, Bipolaris sorokiniana, and Fusarium oxysporum. Both PEE and PEELCN exhibited significant inhibitory effects on the mycelial growth of V. dahliae, B. sorokiniana, and N. dimidiatum, with varying degrees of efficacy. The differential antifungal activity suggests a species-specific mode of action. The findings highlight the promising potential of PEELCN as a sustainable, biocompatible, and cost-effective nanofungicide for the management of plant diseases, with the potential for development into a commercially viable biofungicide for sustainable agriculture.
Neoscytalidium dimidiatum: A newly identified postharvest pathogen of pears and its implications for pome fruits
(Wiley, 2024) Dervis, Sibel; Zholdoshbekova, Sezim; Guney, Inci Guler; Ozer, Goksel
T & uuml;rkiye is a prominent contributor to pear and diverse pome fruit production. Pear fruit with unusual brown to black spots and rot symptoms observed in public marketplaces in Mardin province have raised concerns regarding postharvest fruit health. The consistent isolation of a fungus from these fruits revealed morphological features indicative of Neoscytalidium dimidiatum. Phylogenetic confirmation of its identity ensued through BLASTn searches targeting, the internal transcribed spacer (ITS) of ribosomal DNA, the partial translation elongation factor 1-alpha gene (tef1), and the partial beta-tubulin gene (tub2). Pathogenicity evaluations were conducted on common pome fruits, namely pears, apples, and quinces, unveiling the susceptibility of all examined fruits to postharvest infection by this emergent pathogen. Furthermore, an investigation was carried out to discern the pathogen's response to varying temperature ranges on pear fruits, revealing that the most pronounced lesions occurred at 30 degrees C, followed by 25 degrees C, 35 degrees C, and 20 degrees C. Conversely, no lesion development was observed at 10 degrees C, 15 degrees C, or 40 degrees C. To the best of our knowledge, this study represents the first report of N. dimidiatum as the etiological agent responsible for postharvest rot in pear fruit. The implications of these findings highlight the potential threat posed by this pathogen to pome fruits postharvest, especially in regions where cold storage facilities are not widely utilized, warranting increased vigilance and preventive measures.