Browsing by Author "Jahan, Israt"

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Advances and applications of nanomaterial-assisted phytoremediation
(CRC Press, 2023) Jahan, Israt
Nanotechnology has the potential to drastically transform the agri-food sector with its significant applications to improve agricultural productivity and the efficiency of agrochemicals. The food sector has benefitted from the inclusion of nanoparticles in food matrixes and the nanoencapsulation of nutraceuticals. Smart packaging mate rials designed with the help of nanotechnology have been used for increasing the shelf life of stored food products. Nanomaterials have been extensively used for the delivery of important agrochemicals to enhance their bioefficacy, prevent their deg radation, and control their release. Various nanomaterials have been explored for remediation of arising environmental issues. Nanotechnology has also made a useful contribution to the utilization of huge agricultural and food wastes for production of valuable products. The existing and emerging applications of nanotechnology will contribute to environmental sustainability
Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
(Wiley Online Library, 2022) Aktepe, Necmettin; Baran, Ayşe; Atalar, Mehmet Nuri; Baran, Mehmet Fırat; Keskin, Cumali; Taşkin, Abdullah; Yavuz, Ömer; Demirtaş, İbrahim; Oğuz, Ercan; Jahan, Israt
Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography–electrospray ionization– tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.
Anatolian Medicinal Plants as Potential Antiviral Agents: Bridging Traditional Knowledge and Modern Science in the Fight Against Covid-19 and Related Viral Infections
(Tubitak Scientific & Technological Research Council Turkey, 2024) Tilkat, Engin; Jahan, Israt; Hoser, Ayse; Kaplan, Alevcan; Ozdemir, Oguzhan; Onay, Ahmet
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was the cause of the coronavirus 2019 (COVID-19), commonly known as the coronavirus pandemic. Since December 2020, COVID-19 vaccines have been extensively administered in numerous countries. In addition to new antiviral medications, the treatment regimen encompasses symptom management. Despite sustained research efforts, the outbreak remains uncontrolled, with affected patients still lacking proper treatment. This review is a valuable asset for researchers and practitioners aiming to delve into the yet unexplored potential of Anatolian flora in the fight against COVID-19 and other viral infections. Numerous medicinal plants in Anatolia, such as thyme, sage, cannabis, oregano, licorice root, and Origanum sp., contain bioactive compounds with proven antiviral properties that have been used in the region for centuries. The rich legacy of traditional Anatolian medicine (TAM), has significantly influenced modern medicine; thus, the profusion of medicinal plants native to Anatolia holds promise for antiviral drug development, making this review essential for researchers and practitioners.
The Effects of Silver Nanoparticles (AgNPs) on Thermophilic Bacteria: Antibacterial, Morphological, Physiological and Biochemical Investigations
(MDPI, 2024) Jahan, Israt; Matpan Bekler, Fatma; Tunç, Ahmed; Güven, Kemal
Since thermophilic microorganisms are valuable sources of thermostable enzymes, it is essential to recognize the potential toxicity of silver nanoparticles used in diverse industrial sectors. Thermophilic bacteria Geobacillus vulcani 2Cx, Bacillus licheniformis 3CA, Paenibacillus macerans 3CA1, Anoxybacillus ayderensis FMB1, and Bacillus paralicheniformis FMB2-1 were selected, and their MIC and MBC values were assessed by treatment with AgNPs in a range of 62.5-1500 μg mL-1. The growth inhibition curves showed that the G. vulcani 2Cx, and B. paralicheniformis FMB2-1 strains were more sensitive to AgNPs, demonstrating a reduction in population by 71.1% and 31.7% at 62.5 μg mL-1 and by 82.9% and 72.8% at 250 μg mL-1, respectively. TEM and FT-IR analysis revealed that AgNPs caused structural damage, cytoplasmic leakage, and disruption of cellular integrity. Furthermore, cell viability showed a significant decrease alongside an increase in superoxide radical (SOR; O2-) production. β-galactosidase biosynthesis decreased to 28.8% level at 500 μg mL-1 AgNPs for G. vulcani 2Cx, 32.2% at 250 μg mL-1 for A. ayderensis FMB1, and 38.8% only at 62.5 μg mL-1, but it was completely inhibited at 500 μg mL-1 for B. licheniformis 3CA. Moreover, B. paralicheniformis FMB2-1 showed a significant decrease to 11.2% at 125 μg mL-1. This study is the first to reveal the toxic effects of AgNPs on thermophilic bacteria.
Kitre Sakızının Farklı Kanser Hücreleri Üzerine Proliferatif Etkisi
(2024) Kandemir, Sevgi İrtegün; Jahan, Israt; Teke, Fatma
Kitre sakızı (GT) Astragalus cinsinin Asyatik türlerinin dallarından ve gövdelerinden boşaltılan doğal bitki eksüdasıdır. Heterojen bir polisakkarit olan GT büyük biyouyumluluk, termal kararlılık, biyolojik olarak parçalanabilirlik, hidrofiliklik ve antioksidan aktivite gibi ayırt edici fizikokimyasal ve biyolojik özellikleri nedeniyle çeşitli biyomedikal alanlarda ve geleneksel olarak etnotıpta kullanılmaktadır. Bu çalışmada, GT’nin çeşitli kanser hücre dizileri üzerinde sitotoksik etkilerinin olup olmadığını araştırmayı amaçladık. Bu amaçla insan kolorektal adenokarsinomu (CACO-2), glioblastoma multiforma tümörü (T98G), yumurtalık sarkomu (SKOV-3) ve meme kanseri (MCF-7) hücreleri gibi dört farklı kanser hücre dizisi kullanıldı. GT, solvent olarak hem %5 DMSO hem de dH20 kullanılarak 200 µg/mL, 100 µg/mL, 50 µg/mL, 25 µg/mL ve 12.5 µg/mL konsantrasyonunda hazırlandı. İn vitro sitotoksisite çalışması için MTT (3-(4, 5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür) kolorimetrik deney kullanıldı. Hücre canlılığı yüzdeleri tüm uygulamalar için %80’in üzerinde bulunduğundan, GT’nin bu kanser hücreleri üzerinde sitotoksik etkisi olmadığı bulundu. Ancak, belirli konsantrasyonlarda GT’nin doza bağlı olarak dikkate değer hücre proliferasyonu etkinliği, MCF-7 dışındaki tüm kanser hücrelerinde gözlendi. Sonuç olarak, bu çalışma GT’nin kanser hücrelerinin proliferasyonunu arttırıcı etkisinden dolayı kanser hastalarının GT veya GT içeren ürünlerin kullanımı açısından dikkatli olunması gerektiğini önermektedir.