Browsing by Author "Kandemir, Sevgi Irtegun"

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Citation - WoS: 0
Citation - Scopus: 0
Determination of chemical composition and antioxidant, cytotoxic, antimicrobial, and enzyme inhibition activities of Rumex acetosella L. plant extract
(Springer int Publ Ag, 2024) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Atalar, Mehmet Nuri; Keskin, Cumali; Khalilov, Rovshan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
Purpose The phenolic composition, antioxidant, antimicrobial activity, enzyme inhibition activity, and cytotoxic activity potentials of the plant Rumex acetosella L. (R. acetosella) were examined in this study. Materials and Methods: The chemical composition of R. acetosella methanol extract was identified by the LC-MS/MS method. The antioxidant activity was tested using beta-carotene/linoleic acid, DPPH free radical scavenging, ABTS cation radical scavenging, CUPRAC reducing power, and metal chelating activity methods. The cytotoxic activity was determined by the MTT assay using human ovarian adenocarcinoma (Skov-3), glioblastoma (U87), human dermal fibroblasts (HDF), and human colorectal adenocarcinoma (CaCo-2) cell lines. The antimicrobial activity of methanolic extracts was tested on gram-negative (Escherichia coli and Pseudomonas aeuriginosa) and gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) using the in vitro minimum inhibition concentration method (MIC). Enzyme inhibition activity of R. acetosella methanol extract was measured spectrophotometrically against acetylcholinesterase (AChE) and glutathione S-transferase (GST) enzymes. Results: The findings showed that the major components of the methanol extract content were luteolin-7-O-glucoside (1.599 m/L), polydatin (91,024 m/L), and shikimic acid (0.773 m/L). It was determined that the extract and standard antioxidant (a-tocopherol) results in DPPH center dot, and ABTS center dot + tests performed to determine the antioxidant activity were close to each other, and this value was more effective than the standard antioxidant (alpha-tocopherol) in the CUPRAC test. These results suggested that the plant's antioxidant potential was higher when compared with reference antioxidant compounds. It was determined that the methanol extract of R. acetosella had a weaker effect on the growth of the tested microorganisms than the antibiotics used as standard. The activity of the GST and AChE enzymes was found to be severely inhibited by the methanol extract of R. acetosella. Conclusion: Based on these findings, R. acetosella L. is a medicinal and commercially beneficial plant that warrants further investigation.
Citation - WoS: 6
Rapid Biosynthesis of Silver Nanoparticles By Celtis Tournefortii Lam. Leaf Extract; Investigation of Antimicrobial and Anticancer Activities
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Keskin, Cumali; Baran, Ayşe; Kandemir, Sevgi Irtegun; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
The usage of metallic nanoparticles are very common. Environmentally friendly approaches in obtaining nanoparticles attract a lot of attention because of their advantages. In this study, an easy and economical biosynthesis of silver nanoparticles (AgNPs) was made with the extract of Celtis tournefortii LAM. leaf. For the characterization of synthesized nanoparticles, Spectrophotometer (UV-vis), Transmission Electron Microscope (TEM), Field Emission Scan Electron Microscopy (FE-SEM), Atomic Power Microscopy (AFM), Electron Disperse X-ray (EDX) Fourier Transformation Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), Thermogravimetric and Differential Thermal Analysis (TGA-DTA), Zeta Sizer and Zeta Potential Analysis data were used. As a result of the data analysis, it was determined that the AgNPs had a spherical appearance, an average size distribution of 4.8 nm, had a maximum absorbance at a wavelength of 482.13 nm, a crystal nanosize of 10.95 nm, and a surface charge of-21.6 mV. Inhibition activities of AgNPs on the growth of pathogenic strains were determined by the microdilution method. The results showed that the nanoparticles were effective even at low concentrations. The Minimum Inhibitory Concentration (MIC) value of the tested materials on the growth of the strains was found between 0.03-1.00 mu g mL-1. Anticancer activity of AgNPs was investigated on CaCo-2, U118, Skov3 cancer cell lines and healthy cell line HDF by the MTT method.