Browsing by Author "Kaya, S."

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Comparison of the Gonadoprotective Functions of Nigella Sativa Oil, Metformin and Vitamin E on Streptozotocin-Induced Diabetic Testis
(Edizioni Minerva Medica, 2025) Kaya, S.; Seker, U.; Ozmen, M.F.; Ayaz, H.; Erdogan, G.; Nergiz, Y.
BACKGROUND: Changes in blood glucose levels in DM cause many complications. We aimed to make a detailed comparison study on the protective effects of Nigella sativa oil, metformin, and vitamin E on the histopathologic effects of diabetes on the male reproductive system. METHODS: In the experiment, 30 rats were divided equally into 5 groups: Control, DM, DM+NS, DM+M and DM+E. To induce diabetes in the experimental groups, a one-time injection of 45 mg/kg Streptozotocin was administered. Rats with blood glucose levels above 250 mg/dl were included in the experiment. The experiment period lasted 8 weeks. Weekly body weight of the rats, reproductive organ weights at the end of the experiment, spermatologic data, immunohistochemical staining, TUNEL assay staining and morphometric evaluation were performed. RESULTS: According to Johnsen Biopsy Score and Seminiferous Tubule diameter data, the DM+NS group had similar results to the control group, indicating that Nigella sativa oil preserved the seminiferous tubule diameter structure better than vitamin E and metformin. There were head, neck and tail defects in the diabetes group. Based on the results of the applications carried out to evaluate apoptosis and proliferation in testicular tissue, it can be concluded that the treated diabetic groups, especially the Nigella sativa oil, improved the structure of spermatogenic tubules and also reduced the number of apoptotic cells, as shown by the results of the TUNEL test (P<0.05). CONCLUSIONS: We think that Nigella sativa oil is more effective on the mechanism of diabetes than other active substances and regulates metabolic changes and may be an effective therapeutic agent for diabetes treatment. © 2024 EDIZIONI MINERVA MEDICA.
In Vitro Cytotoxicity and Antioxidant Evaluation of Oak (Quercus sp.) and Hazelnut Shell (Corylus avellana L.) Pyrolized Wood Vinegar: MTT Assay and CUPRAC Results
(Springer Science and Business Media B.V., 2025) Koç, İ.; Sabancilar, İ.; Kaya, S.; Mendeş, M.; Keskin, C.; Eftekhari, A.
Wood vinegar, also known as pyroligneous acid, is an organic liquid byproduct of wood pyrolysis, rich in compounds such as acetic acid, phenols, and ketones. This study aimed to evaluate the antioxidant and anticancer properties of wood vinegar derived from oak (Quercus sp.) residues (WVO) and hazelnut shells (Corylus avellana L.) (WVH) under in vitro conditions. Method: Wood vinegars obtained from oak trash and hazelnut shell waste were tested for biological activity. The antioxidant capacity was assessed using the copper(II) reducing antioxidant capacity (CUPRAC) method. Cytotoxic effects were evaluated using the MTT assay on healthy human endothelial cells (HUVEC) and two cancer cell lines (HT29—colon adenocarcinoma and U2OS—osteosarcoma) at concentrations ranging from 1.25% to 20% (v/v) over 24, 48, and 72-hour exposure periods. The results revealed significant interactions between vinegar type, concentration, and exposure duration. Notably, the 10% and 20% (v/v) concentrations produced significantly different absorbance values compared to lower concentrations across all time points. A dose-dependent decrease in cell viability was observed, particularly in the cancer cell lines, while healthy cells exhibited relatively higher resistance. The study demonstrates that wood vinegar exhibits promising antioxidant and selective anticancer properties, with cytotoxic effects being more pronounced at higher concentrations. These findings suggest that wood vinegar may offer potential as a natural anticancer agent. Further research is warranted to elucidate the underlying mechanisms and to explore their possible therapeutic applications. © The Author(s), under exclusive licence to Springer Nature B.V. 2025.