Browsing by Author "Keskin, Oktay"

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Development and Implementation of a Sandwich ELISA Prototype for the Detection of Brucella Antigens in Cheese Samples
(Elsevier Sci Ltd, 2026) Dellal, Ozden; Gurbuz, Semra; Tel, Osman Yasar; Demir, Muzeyyen; Gurbilek, Sevil Erdenlig; Yucetepe, Ayfer Gullu; Keskin, Oktay
Brucellosis remains one of the most important zoonotic diseases worldwide, posing a serious threat to both public health and animal production. Unpasteurized dairy products represent a major route of transmission. Furthermore, even in regions where the disease has been eradicated, individuals may remain at risk due to travel or the illegal importation of contaminated products. Effective control therefore relies on rapid, accurate, and practical diagnostic methods. In this study, a sandwich enzyme-linked immunosorbent assay (ELISA) prototype was developed to detect Brucella antigens in cheese irrespective of bacterial viability. The assay was evaluated using 150 fresh cheese samples collected from the Central and Siverek districts of S & cedil;anlıurfa province, Türkiye. The developed test demonstrated 100% specificity and 66% sensitivity. Overall, 17.3% of the samples were positive for Brucella antigens, with similar positivity rates observed across both districts. These findings indicate a considerable public health risk associated with contaminated fresh cheese. The newly developed sandwich ELISA may serve as a practical tool for antigen detection and support brucellosis surveillance and control efforts.
Citation - WoS: 12
An Enzyme-Linked Immunosorbent Assay for Brucella Specific Antibody and Real-Time PCR for Detecting Brucella Spp. in Milk and Cheese in Sanliurfa, Turkey
(UNIV AGRICULTURE, FAC VETERINARY SCIENCE, 2017) Altun, Serap Kilic; Yigin, Akin; Gurbilek, Sevil Erdenlig; Gurbuz, Semra; Demirci, Mehmet; Keskin, Oktay; Tel, Osman Yasar
The objective of this study was to investigate the presence of anti-Brucella antibody and Brucella spp. DNA in cow, sheep and goat milk and in Urfa cheese collected from markets and bazaars in Sanliurfa, located in southeast of Turkey. A total of 258 samples consisting of 178 raw milk (48 cow milk, 65 sheep milk and 65 goat milk) samples and 80 Urfa cheese samples were investigated. Anti-Brucella antibody was detected by indirect ELISA (i-ELISA), and the presence of Brucella spp. DNA was screened by real time Polymerase Chain Reaction (RTPCR). 16.6% of the cow, 6.1% of the goat and 6.1% of the sheep milk and 16.25% of the cheese samples were found as positive for brucella antibodies by i-ELISA. The RT-PCR assay amplified Brucella DNA from 18.75, 7.6 and 6.1% cow, goat and sheep milk samples respectively. Brucella DNA was amplified from 22.5% cheese samples. The 11.2% and 13.9% of the samples were found as positive by i-ELISA and RT-PCR respectively. This study indicates that milk and milk products consumed in Sanliurfa poses a risk to public health in terms of brucellosis. The combining usage of both i-ELISA and RT-PCR methods could lead to more reliable results to detect anti-Brucella antibody and Brucella spp. DNA from milk and cheese samples. (C) 2016 PVJ. All rights reserved
Citation - WoS: 2
Citation - Scopus: 2
Presence of Staphylococcus Aureus, Staphylococcal Enterotoxins and Antimicrobial Resistance in Traditionally Produced Raw Milk Cheeses
(M H Schaper GmbH CO KG, 2018) Gurbuz, Semra; Keskin, Oktay; Gurbilek, Sevil Erdenlig; Tel, Osman Yasar; Yigin, Akin; Demirci, Mehmet; Hassan, Hala
The objectives of this study was to investigate the presence of Staphylococcus aureus, distribution of classical staphylococcal enterotoxin (SE) SEA to SEE, relevant gene/s and antimicrobial resistance pattern of S. aureus isolated from traditionally produced raw milk cheeses. A total of 106 fresh white cheese samples were examined. The 25 (23.6 %) of 106 cheese samples were found to be contaminated with coagulase positive staphylococci (CPS). From 52 isolates identified as S. aureus, one or more SEs was detected in 38.4 % of the isolates by ELISA whereas one or more se genes were detected in 50 % of the isolates by RT PCR. SEE (75 %) and see gene (61.5 %) were detected most frequently, whereas SED and sed gene were not detected in any isolates. Overall, 63.5 % of isolates were resistant to antimicrobial agents with 59.6 %, 13.5 %, 5.8 %, 5.8 % and 3.8 % of the isolates were resistant to penicillin, erythromycin, tetracycline, cefoxitin and kanamycin, respectively. The results of this study have revealed that cheeses made from raw milk were highly contaminated with S. aureus, therefore, creates a risk for public health due to the presence of enterotoxins as well as resistant strains against antimicrobial agents.