Browsing by Author "Mermutluoğlu, Çiğdem"

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Investigation of Anti-Neutrophil Cytoplasmic Antibody by Indirect Immunofluorescence Assay in COVID-19 Patients
(Galenos Publ House, 2025) Ayaydın, Zeynep; Özcan, Nida; Atmaca, Selahattin; Ayaydin, Zeynep; Mermutluoğlu, Çiğdem; Tekin, Ali Cem; Department of Basic Medical Sciences / Temel Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Aim: Coronavirus disease-2019 (COVID-19) has been suggested to trigger the production of autoimmune antibodies and contribute to the development of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. This study aims to investigate the presence of ANCA among COVID-19 patients. Methods: This cross-sectional, prospective analysis included 200 COVID-19 patients with positive polymerase chain reaction test results for severe acute respiratory syndrome-coronavirus-2 and no history of autoimmune disease, recruited between June 2021 and November 2022. The control group included 50 age-matched healthy blood donors. The ANCA profile was assessed using the indirect immunofluorescence assay method with the EUROPLUS Granulocyte Mosaic EUROPattern test kit (EUROIMMUN, Germany) on sera samples of the patient and control groups. Results: Perinuclear ANCA (p-ANCA) was detected in 12 of 200 COVID-19 patients (6.0%) and cytoplasmic ANCA (c-ANCA) was detected in 15 of 200 patients (7.5%). No ANCA positivity was observed in the control group (0/50). ANCA positivity among COVID-19 patients (27 of 200, 13.5%) was statistically significantly higher than in the control group (p<0.05). ANCA positivity was significantly higher in intensive care unit (ICU) patients (21 of 77, 27.3%) compared to non-ICU patients (6 of 123, 4.9%) (p<0.05). Conclusion: ANCA presence in ICU patients supports the hypothesis that COVID-19 triggers ANCA synthesis and contributes to disease severity.
An Investigation of the Role of Trace Elements and Biochemical Parameters in Patients With COVID-19
(2025) Erkan, Revşa Evin Canpolat; Unsal, Velıd; Özbek, Erdal; Sabancılar, İlhan; Temız, Hakan; Mermutluoğlu, Çiğdem
Aim: The COVID-19 pandemic is an emergent viral respiratory disease characterized by high fever and shortness of breath, and it was declared a pandemic by the World Health Organization in March 2020. Early assessment of patients’ biochemical tests is important for accelerating diagnosis, allowing effective treatment, and controlling the further spread of the disease. The present study aimed to investigate the association between the disease, trace elements -including copper (Cu), zinc (Zn), selenium (Se), manganese (Mn), and cobalt (Co) vitamin D, Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) biochemical levels, and the correlation between the parameters tested in patients with COVID-19. Methods: In our study, 40 patients (case group) who were hospitalized with a diagnosis of COVID-19 based on chest X-ray images and RT-PCR results evaluated by an infectious diseases specialist were included, along with 40 healthy individuals (control group) over the age of 18 who had no prior symptoms of COVID-19, no visits to a medical doctor for COVID-19, and no history of hospitalization due to the disease. Beckman Coulter AU5800 (Beckman Coulter, Brea, CA, USA) autoanalyzer was used for spectrophotometric analyses of clinical biochemistry tests, and vitamin D levels were examined using the HPLC method with the Shimadzu SIL-20A HT autosampler. Levels of trace elements-including Cu, Zn, Se, Mn, and Co-were measured by inductively coupled plasma mass spectrometry (ICP-MS) on an ICP-MS Bruker Aurora M90 analytical complex. The normal distribution hypothesis for the variables in question was tested using the Kolmogorov–Smirnov test. Student’s t-test was used for intergroup comparisons of variables meeting the normal distribution hypothesis, whereas Mann–Whitney U test was used for variables that did not meet the hypothesis. Results: Vitamin D levels were much lower in the case group (12.05 ng/mL ± 6.27) compared to the control group (23.54 ng/mL ± 10.54), and the difference was statistically significant (p Conclusion: Decreased levels of vitamin D and trace elements (Se, Zn, Mg and Cu) are associated with the development of viral pathogens, including COVID-19, as well as increased ALT and AST parameters. It was concluded that a diet rich in vitamins and trace elements would strengthen the immune system, reduce the rate of virus spread, and slow down the disease aggravation.