Browsing by Author "Sabancilar, Ilhan"

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Citation - Scopus: 5
Does oxidative status affect serum sclerostin levels in patients with type 2 diabetes mellitus?
(Folia Medica, 2023) Unsal, Velid; Ilhan Sabancilar, Fidel Demir, Gulten Toprak, Zafer Pekkolay; Pekkolay, Zafer; Demir, Fidel; Sabancilar, Ilhan; Toprak, Gulten
Introduction: Sclerostin is a glycoprotein known as a negative regulator of bone formation, predominantly expressed by mature osteocytes. There is no causative evidence information on the role of sclerostin in the pathogenesis of type 2 diabetes mellitus (T2DM) in humans. Aim: This study aimed to investigate the relationship between serum sclerostin levels and oxidative status and biochemical parameters in T2DM patients and healthy people. Materials and methods: This cross-sectional study, conducted in a clinical trial center, included 45 subjects with T2DM and 45 subjects as controls. Results: Serum sclerostin, total oxidative status (TOS), albumin, and ferritin levels were significantly higher in T2DM patients than in the control group (p<0.05). Total antioxidant status (TAS) was significantly higher in the control group (p<0.05). There was a weak positive correlation between sclerostin and TOS (r=0.23, p=0.03) and a weak negative correlation between sclerostin and TAS (r=-0.28, p=0.03). Conclusions: We have demonstrated that serum sclerostin levels increase in patients with T2DM and that the increased sclerostin levels are associated with oxidative stress.
Citation - WoS: 1
In Vitro Cytotoxicity and Antioxidant Evaluation of Oak (Quercus sp.) and Hazelnut Shell (Corylus avellana L.) Pyrolized Wood Vinegar: MTT Assay and CUPRAC Results
(Springer, 2025) Koc, Ibrahim; Sabancilar, Ilhan; Kaya, Seckin; Mendes, Mehmet; Keskin, Cumali; Eftekhari, Aziz
Wood vinegar, also known as pyroligneous acid, is an organic liquid byproduct of wood pyrolysis, rich in compounds such as acetic acid, phenols, and ketones. This study aimed to evaluate the antioxidant and anticancer properties of wood vinegar derived from oak (Quercus sp.) residues (WVO) and hazelnut shells (Corylus avellana L.) (WVH) under in vitro conditions. Method: Wood vinegars obtained from oak trash and hazelnut shell waste were tested for biological activity. The antioxidant capacity was assessed using the copper(II) reducing antioxidant capacity (CUPRAC) method. Cytotoxic effects were evaluated using the MTT assay on healthy human endothelial cells (HUVEC) and two cancer cell lines (HT29-colon adenocarcinoma and U2OS-osteosarcoma) at concentrations ranging from 1.25% to 20% (v/v) over 24, 48, and 72-hour exposure periods. The results revealed significant interactions between vinegar type, concentration, and exposure duration. Notably, the 10% and 20% (v/v) concentrations produced significantly different absorbance values compared to lower concentrations across all time points. A dose-dependent decrease in cell viability was observed, particularly in the cancer cell lines, while healthy cells exhibited relatively higher resistance. The study demonstrates that wood vinegar exhibits promising antioxidant and selective anticancer properties, with cytotoxic effects being more pronounced at higher concentrations. These findings suggest that wood vinegar may offer potential as a natural anticancer agent. Further research is warranted to elucidate the underlying mechanisms and to explore their possible therapeutic applications.
Role of vitamin D, folic acid, ferritin, inflammation and oxidative stress in the pathogenesis of COVID-19
(Progress in Nutrition, 2022) Unsal, Velid; Ilhan Sabancilar, Erdal Ozbek, Cigdem Mermutluoglu, Hakan Temiz; Ozbek, Erdal; Mermutluoglu, Cigdem; Temiz, Hakan; Sabancilar, Ilhan
Abstract. The COVID-19 pandemic is one of the most devastating and significant events of recent times. COVID-19 has so far become one of the worst infectious disease outbreaks of recent times, with more than 635 million cases and more than 6.6 million deaths. Viruses cause an explosion of inflammatory cytokines and reactive oxygen types. Oxidative stress is thought to have a key role in COVID-19. vitamin D, folic acid, calcium (Ca), magnesium (Mg) and ferritin levels are thought to be associated with COVID-19. This study aims to investigate the role of oxidative stress, inflammation, vitamin D and folic acid, ferritin, Ca and Mg in the pathogenesis of COVID-19. Materials and Methods: 45 patients diagnosed with COVID-19 and 45 healthy persons (control group) were included in the study. Vitamin D, ferritin, folic acid, CRP, Ca, Mg and Phosphorus were measured in an autoanalyzer, and SOD, GSH-Px and MDA were spectrophotometrically measured in the serum of the participants. TNF-α, IL-1β and IL6 levels were studied by the ELISA method. Results: The activity of SOD, GSH-px, antioxidant enzymes, Serum vitamin D, folic acid, Ca and Mg of the COVID-19 group was found to be significantly lower than the control group (p<0.05). Conclusion: Again, the levels of MDA, TNF-α, IL-1β, IL-6, CRP and ferritin in the Covid-19 group were found to be significantly higher than in the control group (p<0.05).Antioxidant enzyme activities were low and oxidative stress was high in patients with COVID-19. At the same time, the levels of serum ferritin, CRP, TNF-α, IL-1β and IL6 were high, and levels of Ca and Mg were low in patients with COVID-19.According to these results, we hypothesize think that the level of oxidative stress, inflammation, vitamin D, and serum ferritin, Ca, and Mg levels play a role in the pathogenesis of COVID-19. Future clinical trials should be conducted to further clarify the pathogenesis in patients with COVID-19.
Citation - WoS: 1
Citation - Scopus: 1
Synthesis, Spectroscopic Characterization, and Biological Evaluation of a Novel Acyclic Heterocyclic Compound: Anticancer, Antioxidant, Antifungal, and Molecular Docking Studies
(MDPI, 2025) Alhilal, Mohammad; Alhilal, Suzan; Sabancilar, Ilhan; Gomha, Sobhi M.; Elhenawy, Ahmed A.; Ouf, Salama A.
Background/Objectives: This study aimed to synthesize a novel, high-molecular-weight acyclic heterocyclic compound, compound 5, via a one-pot reaction between Trichloroisocyanuric acid (TCCA) and ethanolamine, and evaluate its anticancer, antioxidant, and antifungal activities. Methods: Its complex tetrameric structure, assembled through N-N linkages, was unequivocally confirmed by a full suite of spectroscopic techniques including IR, 1H & 13C NMR, 2D-NMR, and high-resolution mass spectrometry (LC/Q-TOF/MS). The MTT assay was used to assess the anticancer activity of compound 5 against four different human cancer cell lines. Results: The findings indicate that human colon (HT29) and ovarian (OVCAR3) cancer cells were sensitive to the treatment, whereas brain (glioblastoma) (T98G) cancer cells were resistant. The most pronounced cytotoxic effect was observed in pancreatic (MiaPaCa2) cancer cells. Notably, compound 5 exhibited potent antifungal properties, achieving 100% inhibition of the pathogenic water mould Saprolegnia parasitica zoospores at 100 mu M after 10 min. Molecular docking studies corroborated the biological data, revealing a high binding affinity for key cancer and fungal targets (Thymidylate Synthase and CYP51), providing a strong mechanistic basis for its observed activities. Conclusions: These findings establish compound 5 as a promising dual-action agent with significant potential as both a targeted anticancer lead and an eco-friendly antifungal for applications in aquaculture.