Browsing by Author "Savas, Hasan Basri"

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Protective Effect of Astaxanthin on Histopathologic Changes Induced by Bisphenol a in the Liver of Rats
(Univ Agriculture, Fac veterinary Science, 2024) Karabekir, Seda Cetinkaya; Gultekin, Burcu; Ayan, Ilknur Cinar; Savas, Hasan Basri; Cuce, Gokhan; Kalkan, Serpil
Bisphenol A (BPA) has several potential uses, including in polycarbonate plastics and epoxy resins, which could expose humans to it. Recognized for its hepatotoxicity and ability to accumulate in organs. We prompted this study to explore the hepatoprotective potential of astaxanthin (ASTX), an antioxidant against BPA toxicity. We used 32 male Wistar Albino rats and randomly assigned them as: Control, Sham (olive oil), BPA, and BPA+ASTX. At the end of the experiment, Native Thiol, Total Thiol, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured in serum samples. Histopathological scoring was performed to evaluate the changes caused by ASTX in the liver. Caspase 3 and caspase 9 expression in liver tissues was demonstrated immunohistochemically and by PCR. Collagen I (COL1A1) and collagen III (COL3A1) mRNA levels were measured by PCR in the tissue samples. The BPA group showed elevated AST and ALT with decreased Thiol levels. ASTX administration reversed these changes as observed by reduced AST and ALT levels and increased Thiol levels. Histopathology indicated increased liver damage and fibrosis in the BPA group which were alleviated in the BPA+ASTX group. Gene expression analyses revealed upregulated COL1A1 and COL3A1 in BPA, which was downregulated with ASTX. Immunohistochemistry and PCR confirmed BPA-induced caspase 3 and caspase 9 expression, which were attenuated by ASTX. This study underscores ASTX's hepatoprotective efficacy against BPA-induced hepatotoxicity which ultimately attributed to its antioxidant and antiapoptotic properties. Consequently, ASTX emerges as a promising therapeutic agent for preventing and treating BPA-related liver diseases.
Protective Effects of Selenium Against Acrylamide-Induced Hepatotoxicity in Rats
(Univ Agriculture, Fac veterinary Science, 2024) Sozen, Mehmet Enes; Savas, Hasan Basri; Cuce, Gokhan
Acrylamide (ACR) is an organic chemical widely consumed worldwide, depending on the diet. ACR has toxic effects on the liver and other organs due to oxidative damage. The research is aimed to determine the effects of Selenium (Se) against ACR toxicity. 32 Wistar albino male rats were divided into Control, ACR, Se, and ACR+Se groups. After slaughter on the 28 th day, the blood samples taken from the animals were tested for total oxidant status (TOS) and total antioxidant status (TAS) to assess oxidative stress. The liver tissue sections were evaluated for lymphocyte infiltration, hepatocyte degeneration, sinusoid dilatation, and congestion. IL-6, Bax, and Bcl-2 expression were evaluated with immunohistochemistry. While the ACR group's TOS and oxidative stress index (OSI) values were significantly higher than the control group's, there was no significant difference in the ACR+Se group's TOS and OSI values. The ACR group had a considerably higher histopathological score than the other groups. ACR increased IL-6, and Bax levels and decreased Bcl-2 levels compared to the control, Se, and ACR+Se groups. ACR increased oxidative stress significantly caused toxic effects, inflammation, and cell death in the liver. On the other hand, Se oral supplementation may protect against oxidative stress, toxic effects, inflammation, and cell death induced by ACR in the liver.
Thiol–disulfide balance and trace element levels in patients with seasonal allergic rhinitis
(African Health Sciences, 2022) Savas, Hasan Basri; Gunizi, Huseyin
Abstract Background: The prevalence of allergic diseases is gradually increasing worldwide. The most common such allergic disease is allergic rhinitis (AR). Objective: The present study investigated the possible relationship between seasonal AR and the thiol-disulfide balance and zinc and copper levels in adult individuals. Study Design and Methods: 130 male and female adults were included in the study. The participants’ serum thiol-disulfide balance and zinc and copper levels were measured spectrophotometrically using commercial kits. Statistical significance was accepted as p < 0.05 between the groups. Results: The serum copper (p = 0.001), native thiol (p = 0.006), reduced thiol (p < 0.001), and thiol oxidation reduction ratio (p < 0.001) levels were significantly lower in the seasonal AR group than in the control group. Conclusion: In AR patients, the low level of copper, which is an important trace element, the deterioration of the thiol-disulfide balance, which represents a unique indicator of the oxidant-antioxidant balance, the increased disulfide level caused by oxidative stress, and the decreased native thiol level can all serve as important biochemical markers.