Browsing by Author "Savas, Hasan Basri"

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The Ameliorative Effects of Hesperidin in Rats Developed Hepatotoxicity With Deltamethrin
(Mashhad Univ Med Sciences, 2025) Karabekir, Seda Cetinkaya; Sozen, Mehmet Enes; Ayan, Ilknur Cinar; Savas, Hasan Basri; Cuce, Gokhan; Kalkan, Serpil
Objective(s): Deltamethrin (DLM) is a widely used insecticide in agriculture; however, exposure to it can lead to serious health problems. This study aimed to evaluate the protective effects of hesperidin (HSP), a natural antioxidant, against DLM-induced liver toxicity. Materials and Methods: Thirty-two male Wistar Albino rats (250-300 g, 4 months old) were divided into four groups. The control group received 1 ml of corn oil via oral gavage for 30 days. The DLM group received 1.28 mg/kg DLM in corn oil for 30 days. The DLM+HSP 100 mg/kg and DLM+HSP 300 mg/kg groups received 1.28 mg/kg DLM followed by 100 mg/kg or 300 mg/kg HSP in distilled water, respectively, 30 min after DLM administration for 30 days. Liver tissues were examined histopathologically. Masson's trichrome staining and PCR assessed fibrosis. Caspase 3 and 9 expressions in liver tissues were determined by immunohistochemistry and PCR. Biochemical analyses were conducted on serum samples. Results: HSP supplementation led to a dose-dependent decrease in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. DLM exposure decreased antioxidant capacity, while HSP supplementation increased it dose-dependently. Histopathological evaluations showed increased liver damage in the DLM group, while HSP administration reduced liver toxicity. Masson's trichrome staining and analysis of collagen I (COL1A1) and collagen III (COL3A1) gene expression revealed increased fibrosis in the DLM group, which was attenuated with HSP treatment. Conclusion: The potential prevention of DLM-induced liver toxicity and apoptosis by HSP may be an alternative protective strategy.
Comparison of growth factor levels in injectable platelet-rich fibrin obtained from healthy individuals and patients with chronic periodontitis: a pilot study
(Bmc, 2024) Karci, Bilge; Savaş, Hasan Basri; Savas, Hasan Basri
Background This study aimed to assess and compare the concentrations of growth factors, white blood cells (WBCs), and platelets in injectable platelet-rich fibrin (i-PRF) derived from people with healthy periodontal conditions and those with chronic periodontitis.Methods Venous blood samples were obtained from 30 patients diagnosed with chronic periodontitis (test group) and 30 participants with healthy periodontal conditions (control group). The i-PRF was then acquired from centrifuged blood. The growth factors (VEGF, IGF-1, TGF-beta 1, PDGF-BB and EGF) released from the i-PRF samples were compared between groups with ELISA testing. The amounts of WBCs and platelets were also compared.Results No significant differences in the concentrations of growth factors were found between the groups (the mean values for the control and test groups were, respectively: IGF: 38.82, 42.46; PDGF: 414.25, 466.28; VEGF: 375.69, 412.18; TGF-beta 1: 21.50, 26.21; EGF: 138.62, 154.82). The test group exhibited a significantly higher WBC count than the control group (8.80 vs. 6.60, respectively). However, the platelet count did not show a statistically significant difference between the groups (control group 242.0 vs. test group 262.50). No significant correlation was observed between WBC count and growth factor level in either group.Conclusions The growth factor levels in i-PRFs did not exhibit significant difference between the two groups. This suggests that the levels of these growth factors may be unaffected by the periodontal disease.
Effects of Trimetazidine on Oxidant-Antioxidant Balance and Angiogenesis; an in Vivo Experimental Study
(BMC, 2025) Kayan, Fethullah; Savas, Hasan Basri
Background We evaluated the effects of trimetazidine (TMZ) on the oxidative-antioxidative balance and angiogenesis in an in vivo experimental model. This study aims to evaluate the effects of trimetazidine on angiogenesis through histological analysis and to assess its impact on oxidative-antioxidative balance through biochemical measurements. Methods In this study, Ross 308 breed chicken eggs (n = 40) were used, and embryos were divided into four distinct groups. On the eighth day of incubation, the vascular density of the embryos was examined. Following the assessment of vascular development, 4-5 mL of albumin was collected via syringe to measure oxidative stress markers. Each group consisted of 10 embryos, with a total of 40 embryos used in the study. The groups were organized as follows: Control Group (CG), Bevacizumab Group (BC), Trimetazidine 10(-)(4) Group, and Trimetazidine 10(-)(5) Group. Results When the total oxidative capacity (TOC) levels were compared among the groups, the bevacizumab group exhibited significantly higher values than the control group (p < 0.05). In oxidative stress index (OSI) measurements, the bevacizumab group also showed significantly higher values compared to the control group (p < 0.05). In contrast, when the total antioxidant capacity (TAC) levels were compared, both the Trimetazidine 10(-)(4) and Trimetazidine 10(-)(5) groups demonstrated significantly higher values than the control group (p < 0.05). Regarding angiogenesis scoring, the bevacizumab group exhibited a significant anti-angiogenic effect compared to the control group. However, no statistically significant difference was observed between the Trimetazidine 10(-)(4) and Trimetazidine 10(-)(5) groups and the control group (p > 0.05). Conclusion Trimetazidine demonstrated significant antioxidant activity in an in vivo Chorioallantoic Membrane (CAM) model at both 10(-)(4) M and 10(-)(5) M concentrations. However, no positive or negative effects on angiogenesis were detected. We believe that the real-time observation of angiogenesis in our study provided significant value to our research.
Evaluation of Thiol/Disulfide Homeostasis and Ischemia Modified Albumin as Potential Markers for Periodontitis
(BMC, 2025) Karci, Bilge; Savas, Hasan Basri
Background The current study aimed to assess the impact of periodontitis on oxidative stress parameters by examining serum total antioxidant capacity (TAS), total oxidant status (TOS), oxidative stress index (OSI), thiol/ disulfide homeostasis and ischemia modified albumin (IMA). Methods The study had 90 participants, categorized into 3 groups: Group 1: Periodontally healthy; Group 2: Stage II Grade B periodontitis; Group 3: Stage III and IV Grade B periodontitis. Demographic and periodontal variables were assessed. The levels of serum TAS, TOS, OSI, IMA, and thiol/disulfide were assessed. Results No significant differences in sex and age were detected among the groups (p > 0.05). When compared to Group 1, all clinical measurements were statistically significantly greater in Group 3 (p < 0.05). Statistical analysis revealed no significant differences in serum TAS, TOS, and OSI levels among the groups (p > 0.05). The highest serum IMA value was observed in Group 3 (p = 0.037), whereas native thiol (p = 0.00), total thiol (p = 0.00) and disulfide values (p = 0.023) were highest in Group 1. Conclusions These findings indicate that thiol/disulfide homeostasis and IMA could hold promise as a potential biomarker of inflammation in periodontitis.
Protective Effect of Astaxanthin on Histopathologic Changes Induced by Bisphenol a in the Liver of Rats
(Univ Agriculture, Fac veterinary Science, 2024) Karabekir, Seda Cetinkaya; Gultekin, Burcu; Ayan, Ilknur Cinar; Savas, Hasan Basri; Cuce, Gokhan; Kalkan, Serpil
Bisphenol A (BPA) has several potential uses, including in polycarbonate plastics and epoxy resins, which could expose humans to it. Recognized for its hepatotoxicity and ability to accumulate in organs. We prompted this study to explore the hepatoprotective potential of astaxanthin (ASTX), an antioxidant against BPA toxicity. We used 32 male Wistar Albino rats and randomly assigned them as: Control, Sham (olive oil), BPA, and BPA+ASTX. At the end of the experiment, Native Thiol, Total Thiol, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured in serum samples. Histopathological scoring was performed to evaluate the changes caused by ASTX in the liver. Caspase 3 and caspase 9 expression in liver tissues was demonstrated immunohistochemically and by PCR. Collagen I (COL1A1) and collagen III (COL3A1) mRNA levels were measured by PCR in the tissue samples. The BPA group showed elevated AST and ALT with decreased Thiol levels. ASTX administration reversed these changes as observed by reduced AST and ALT levels and increased Thiol levels. Histopathology indicated increased liver damage and fibrosis in the BPA group which were alleviated in the BPA+ASTX group. Gene expression analyses revealed upregulated COL1A1 and COL3A1 in BPA, which was downregulated with ASTX. Immunohistochemistry and PCR confirmed BPA-induced caspase 3 and caspase 9 expression, which were attenuated by ASTX. This study underscores ASTX's hepatoprotective efficacy against BPA-induced hepatotoxicity which ultimately attributed to its antioxidant and antiapoptotic properties. Consequently, ASTX emerges as a promising therapeutic agent for preventing and treating BPA-related liver diseases.
Protective Effects of Selenium Against Acrylamide-Induced Hepatotoxicity in Rats
(Univ Agriculture, Fac veterinary Science, 2024) Sozen, Mehmet Enes; Savas, Hasan Basri; Cuce, Gokhan
Acrylamide (ACR) is an organic chemical widely consumed worldwide, depending on the diet. ACR has toxic effects on the liver and other organs due to oxidative damage. The research is aimed to determine the effects of Selenium (Se) against ACR toxicity. 32 Wistar albino male rats were divided into Control, ACR, Se, and ACR+Se groups. After slaughter on the 28 th day, the blood samples taken from the animals were tested for total oxidant status (TOS) and total antioxidant status (TAS) to assess oxidative stress. The liver tissue sections were evaluated for lymphocyte infiltration, hepatocyte degeneration, sinusoid dilatation, and congestion. IL-6, Bax, and Bcl-2 expression were evaluated with immunohistochemistry. While the ACR group's TOS and oxidative stress index (OSI) values were significantly higher than the control group's, there was no significant difference in the ACR+Se group's TOS and OSI values. The ACR group had a considerably higher histopathological score than the other groups. ACR increased IL-6, and Bax levels and decreased Bcl-2 levels compared to the control, Se, and ACR+Se groups. ACR increased oxidative stress significantly caused toxic effects, inflammation, and cell death in the liver. On the other hand, Se oral supplementation may protect against oxidative stress, toxic effects, inflammation, and cell death induced by ACR in the liver.
Thiol–disulfide balance and trace element levels in patients with seasonal allergic rhinitis
(African Health Sciences, 2022) Savas, Hasan Basri; Gunizi, Huseyin
Abstract Background: The prevalence of allergic diseases is gradually increasing worldwide. The most common such allergic disease is allergic rhinitis (AR). Objective: The present study investigated the possible relationship between seasonal AR and the thiol-disulfide balance and zinc and copper levels in adult individuals. Study Design and Methods: 130 male and female adults were included in the study. The participants’ serum thiol-disulfide balance and zinc and copper levels were measured spectrophotometrically using commercial kits. Statistical significance was accepted as p < 0.05 between the groups. Results: The serum copper (p = 0.001), native thiol (p = 0.006), reduced thiol (p < 0.001), and thiol oxidation reduction ratio (p < 0.001) levels were significantly lower in the seasonal AR group than in the control group. Conclusion: In AR patients, the low level of copper, which is an important trace element, the deterioration of the thiol-disulfide balance, which represents a unique indicator of the oxidant-antioxidant balance, the increased disulfide level caused by oxidative stress, and the decreased native thiol level can all serve as important biochemical markers.