Browsing by Author "Yeken, Mehmet Zahit"

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    Development of an LSU rRNA-Targeted qPCR Assay and Transcriptional Profiling of Defense-Related Genes to Elucidate Barley Resistance to Bipolaris Sorokiniana
    (Academic Press Ltd- Elsevier Science Ltd, 2025) Alkan, Mehtap; Bozoglu, Tugba; Yeken, Muberra; Yeken, Mehmet Zahit; Emiralioglu, Orkun; Tekin, Fatih; Ozer, Goksel
    Spot blotch, caused by Bipolaris sorokiniana, severely limits global barley production. This study characterized five isolates from Bolu, T & uuml;rkiye, and screened 95 barley cultivars for resistance to the most aggressive isolate (B_BS01) using a 1-9 disease severity scale. Cultivars Gazda, Dara, Hilal, Nonius, and Bravo exhibited the highest resistance (Disease severity index: 35.00 %-36.67 %), forming a statistically distinct group. A quantitative PCR assay targeting the LSU rRNA locus of B. sorokiniana was developed, detecting pathogen DNA down to 0.1 pg with high specificity. This assay quantified starkly different colonization dynamics: pathogen DNA was effectively suppressed in resistant cultivars (Dara, Gazda), while it proliferated rapidly in susceptible ones (Merit, B & uuml;lb & uuml;l), resulting in up to 15-fold higher pathogen loads by 4 days post-inoculation. Temporal expression profiling of defense-related genes (PR1, PR2, PR3, PR5, PR10, CSD, LOX, PAL) was conducted in Dara and Merit. Notably, PR1 and PR10 were more strongly induced in Merit (17.06- and 10.56-fold at 72 h post-inoculation), whereas PR3 was preferentially upregulated in Dara. PR5 and LOX were downregulated in both cultivars; CSD showed moderate induction, and PR2 remained relatively stable. The combination of a sensitive qPCR assay and gene expression profiling provides robust tools for resistance screening and supports targeted breeding for spot blotch resistance in barley.
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    Citation - WoS: 35
    Citation - Scopus: 36
    Phylogenetic and Taxonomic Relationship of Turkish Wild and Cultivated Emmer (Triticum Turgidum Ssp. Dicoccoides) Revealed by Ipbs-Retrotransposons Markers
    (Friends Science Publishers, 2019) Aktaş, Hüsnü; Nadeem, Muhammad Azhar; Aktas, Husnu; Yeken, Mehmet Zahit; Zencirci, Nusret; Nawaz, Muhammad Amjad; Baloch, Faheem Shehzad; 16.02. Department of Seed Production / Tohumculuk Teknolojisi Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
    Wild emmer (Triticum turgidum ssp. dicoccoides) is the progenitor of cultivated wheat. Turkey is the main center of wheat and plays a vital role in the spread of various crops among the continents. Karacadag region is considered as the domestication center of wheat and still, hundreds of landraces are prevalent. A total of 29 wild emmer landraces, 4 cultivated emmer wheat (T. turgidum ssp. dicoccum) and five durum wheat (T. turgidum ssp. durum) cultivars were investigated for the genetic diversity and phylogenetic relationship using the iPBS-retrotransposons markers. Mean polymorphism and polymorphic information contents (PIC) were 87.85% and 0.660, respectively. Mean effective numbers of alleles (1.961), Shannon's Information Index (0.682) and gene diversity (0.489) reflected the occurrence of a great level of variations. T17 and Chermik-1 genotypes were found much distinct and breeding valuable genotypes for wheat breeding. The unweighted pair-group method with arithmetic means (UPGMA) divided all genotypes by their genetic makeup and geographical locations. Among 3 species, UPGMA based clustering clearly separated the durum wheat from wild emmer and cultivated emmer wheat. Results are clearly supported by the principal coordinate analysis (PCoA) and model-based structure algorithm. Information provided herein comprehensively reflected the power of iPBS-retrotransposons for the diversity and phylogenetic relationship investigation and reflected that this marker system can be effectively applied to investigate phylogenetic and taxonomic relationship in any crop due to its universal nature. (C) 2019 Friends Science Publishers