Browsing by Author "Yeken, Muberra"

Now showing 1 - 2 of 2

Development of an LSU rRNA-Targeted qPCR Assay and Transcriptional Profiling of Defense-Related Genes to Elucidate Barley Resistance to Bipolaris Sorokiniana
(Academic Press Ltd- Elsevier Science Ltd, 2025) Alkan, Mehtap; Bozoglu, Tugba; Yeken, Muberra; Yeken, Mehmet Zahit; Emiralioglu, Orkun; Tekin, Fatih; Ozer, Goksel
Spot blotch, caused by Bipolaris sorokiniana, severely limits global barley production. This study characterized five isolates from Bolu, T & uuml;rkiye, and screened 95 barley cultivars for resistance to the most aggressive isolate (B_BS01) using a 1-9 disease severity scale. Cultivars Gazda, Dara, Hilal, Nonius, and Bravo exhibited the highest resistance (Disease severity index: 35.00 %-36.67 %), forming a statistically distinct group. A quantitative PCR assay targeting the LSU rRNA locus of B. sorokiniana was developed, detecting pathogen DNA down to 0.1 pg with high specificity. This assay quantified starkly different colonization dynamics: pathogen DNA was effectively suppressed in resistant cultivars (Dara, Gazda), while it proliferated rapidly in susceptible ones (Merit, B & uuml;lb & uuml;l), resulting in up to 15-fold higher pathogen loads by 4 days post-inoculation. Temporal expression profiling of defense-related genes (PR1, PR2, PR3, PR5, PR10, CSD, LOX, PAL) was conducted in Dara and Merit. Notably, PR1 and PR10 were more strongly induced in Merit (17.06- and 10.56-fold at 72 h post-inoculation), whereas PR3 was preferentially upregulated in Dara. PR5 and LOX were downregulated in both cultivars; CSD showed moderate induction, and PR2 remained relatively stable. The combination of a sensitive qPCR assay and gene expression profiling provides robust tools for resistance screening and supports targeted breeding for spot blotch resistance in barley.
Neoscytalidium Dimidiatum as a Postharvest Pathogen Affecting Solanaceous Vegetables
(Wiley, 2025) Derviş, Sibel; Ozer, Goksel; Turkolmez, Sahimerdan; Turkkan, Muharrem; Dervis, Sibel; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Neoscytalidium dimidiatum, a member of the Botryosphaeriaceae family, is an emerging global plant pathogen. Although recently reported on various hosts in T & uuml;rkiye, its impact on commercially available vegetables remained undocumented. This study provides the first report of N. dimidiatum causing postharvest decay in the eggplant (Solanum melongena L.), pepper (Capsicum annuum), potato (Solanum tuberosum), and tomato (Solanum lycopersicum) obtained from local Turkish markets in 2023. The pathogen was identified through morphological characterisation and molecular analysis targeting the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (tef1), and beta-tubulin (tub2) gene sequences. The pathogenicity of N. dimidiatum was confirmed through Koch's postulates at 28 degrees C, and its temperature-dependent effects were assessed on solanaceous vegetables. Disease progression, measured by the area under the disease progress curve (AUDPC), was strongly influenced by temperature. No disease was observed at 12 degrees C. At 18 degrees C, limited disease development occurred in the eggplant and potato. AUDPC values increased significantly at 25 degrees C, generally peaking at 30 degrees C, with some hosts showing similar levels of disease severity at 35 degrees C. These findings highlight the critical role of postharvest temperature control, particularly rapid cooling and cold storage, in minimising losses caused by N. dimidiatum. This is the first report of N. dimidiatum as a postharvest pathogen affecting the eggplant, pepper, tomato fruits, and potato tubers, and the first record of this pathogen on the eggplant and pepper globally, expanding its known host range and reinforcing its significance as an emerging threat to global agriculture.