Browsing by Author "Yildiz, Songul Cetik"

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Citation - WoS: 0
Citation - Scopus: 0
Examination of the Effects of Kefir on Healing Factors in a Mice Burn Model Infected With E. Coli, S. Aureus and P. Aeruginosa Using Qrt-Pcr (Vol 49, Pg 425, 2023)
(Elsevier Sci Ltd, 2025) Demir, Cemil; Demir, Cemil; Ayhanci, Adnan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Citation - WoS: 4
Citation - Scopus: 3
A Histopathological, Immunohistochemical and Biochemical Investigation on the in Vitro Antioxidant, Myeloprotective, Hematoprotective and Hepatoprotective Effects of Hypericum Triquetrifolium Seed Extract Against Cyclophosphamide-Induced Toxicity
(Inst Tecnologia Parana, 2019) Keskin, Cumali; Yildiz, Songul Cetik; Keskin, Cumali; Çetik Yıldız, Songül; Sahinturk, Varol; Ayhanci, Adnan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
The aim of this study was to investigate in vitro antioxidant properties and in vivo protective effects of the methanol extract of the Hypericum triquetrifolium Turra (HT) seed against acute hepatotoxicity, myelotoxicity and hematotoxicity in rats induced by cyclophosphamide (CP). In order to investigate in vivo protective effects of the HT extract on rat tissues, the rats were divided into nine groups. The toxic effects of CP and the protective effects of HT extract on nucleated cells that are produced by bone marrow, serum alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and oxidative stress index (OSI) levels were investigated biochemically. Additionally, liver tissue samples were examined for histopathological changes and apoptosis by Bcl-2, Bax and caspase-3 immunohistochemistry. The results of this study show that HT seed methanol extract has high total phenolic content (179.52 mu g GAE/mg) and antioxidant activity (87.48% in 500 mu g/mL concentration). CP administration caused hepatotoxicity, myelotoxicity and hematotoxicity in the rats. Whereas, the groups of rats that were injected with different concentrations of HT (25, 50 and 100 mg/kg) and CP (150 mg/kg) showed significant protective effects on bone marrow nucleated cells and important decreases on serum ALT, ALP, LDH and OSI levels were observed when compared with the CP injected group.
Citation - WoS: 3
Citation - Scopus: 4
In Vitro Antitumor and Antioxidant Capacity as Well as Ameliorative Effects of Fermented Kefir on Cyclophosphamide-Induced Toxicity on Cardiac and Hepatic Tissues in Rats
(Mdpi, 2024) Demir, Cemil; Irmak, Halit; Cengiz, Mustafa; Irmak, Halit; Cengiz, Betul Peker; Ayhanci, Adnan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Computer Engineering / Bilgisayar Mühendisliği Bölümü
Fermented prebiotic and probiotic products with kefir are very important to slow down and prevent the growth of tumors and to treat cancer by stimulating the immune response against tumor cells. Cyclophosphamide (CPx) is widely preferred in cancer treatment but its effectiveness in high doses is restricted because of its side effects. The aim of this study was to investigate the protective effects of kefir against CPx-induced heart and liver toxicity. In an experiment, 42 Wistar albino rats were divided into six treatment groups: the control (Group 1), the group receiving 150 mg/kg CPx (Group 2), the groups receiving 5 and 10 mg/kg kefir (Groups 3 and 4) and the groups receiving 5 and 10 mg/kg kefir + CPx (Group 5 and 6). Fermented kefirs obtained on different days by traditional methods were mixed and given by gavage for 12 days, while a single dose of CPx was administered intraperitoneally (i.p.) on the 12th day of the experiment. It was observed that alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatinine kinase-MB (CK-MB), ischemia modified albumin (IMA) and Troponin I values, which indicate oxidative stress, increased in the CPx-administered group, and this level approached that of the control in the CPx + kefir groups. Likewise, as a result of the kefir, the rats' CPx-induced histopathological symptoms were reduced, and their heart and liver tissue were significantly improved. In conclusion, it was observed that kefir had a cytoprotective effect against CPx-induced oxidative stress, hepatotoxicity and cardiotoxicity, bringing their biochemical parameters closer to those of the control by suppressing oxidative stress and reducing tissue damage.
Citation - WoS: 3
Investigation of Uroprotective Effects of Seed Methanol Extracts of Hypericum Triquetrifolium Turra. on Cyclophosphamide-Induced Bladder Hemorrhagic Cystitis and Nephrotoxicity in Wistar Albino Rats
(Cukurova Univ, Fac Medicine, 2020) Keskin, Cumali; Çetik Yıldız, Songül; Sahinturk, Varol; Ayhanci, Adnan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Purpose: This study investigated the possible uroprotective effects of Hypericum triquetrifolium Turra. (HT) seed methanol extracts (25,50,100 mg/kg, i.p., for 6 days) against cyclophosphamide (CYP)-induced (150 mg/kg, single dose, i.p.) acute bladder hemorrhagic cystitis (HC) and nephrotoxicity in rats. Materials and Methods: Wistar albino rats used in this study were divided into nine groups, each including seven rats. Group 1 (control) was treated with 0.5ml saline (SF) and Group 2 was treated with CYP (150 mg/kg). Groups 3, 4, 5 were treated with 25, 50, 100 mg/kg HT, respectively while groups 6, 7, 8 were treated with 25, 50, 100 mg/kg CYP + HT, respectively. Finally, Group 9 (control-2) was treated with 0.5ml-%0.2 dimethyl sulfoxide (DMSO). The serum creatinine, blood urea nitrogen (BUN), superoxide dismutase (SOD) and catalase (CAT) levels were measured in blood serum. Results: The CYP-treated rats histopathologically had mild-moderate bladder and renal injuries. The serum creatinine and BUN levels, which are the biochemical markers of renal injury, significantly increased compared to the control group. Conclusion: HT showed a protective effect on CYPrelated bladder HC and nephrotoxicity in rats by inhibiting inflammation and apoptosis.
Citation - WoS: 2
Citation - Scopus: 2
The Protection Afforded by Kefir Against Cyclophosphamide Induced Testicular Toxicity in Rats by Oxidant Antioxidant and Histopathological Evaluations
(Nature Portfolio, 2024) Demir, Cemil; Irmak, Halit; Cengiz, Mustafa; Irmak, Halit; Cengiz, Betul Peker; Ayhanci, Adnan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Computer Engineering / Bilgisayar Mühendisliği Bölümü
Cyclophosphamide (CTX) is the most commonly used effective alkylating drug in cancer treatment, but its use is restricted because its toxic side effect causes testicular toxicity. CTX disrupts the tissue redox and antioxidant balance and the resulting tissue damage causes oxidative stress. In our study based on this problem, kefir against CTX-induced oxidative stress and testicular toxicity were investigated. Rats were divided into 6 groups: control, 150 mg/kg CTX, 5 and 10 mg/kg kefir, 5 and 10 mg/kg kefir + 150 CTX. While the fermented kefirs were mixed and given to the rats for 12 days, CTX was given as a single dose on the 12th day of the experiment. Testis was scored according to spermatid density, giant cell formation, cells shed into tubules, maturation disorder, and atrophy. According to our biochemical findings, the high levels of total oxidant status (TOS), and the low levels of total antioxidant status (TAS) in the CTX group, which are oxidative stress markers, indicate the toxic effect of CTX, while the decrease in TOS levels and the increase in TAS levels in the kefir groups indicate the protective effect of kefir. In the CTX-administered group, tubules with impaired maturation and no spermatids were observed in the transverse section of the testicle, while in the kefir groups, the presence of near-normal tubule structures and tubule lumens despite CTX showed the protective effect of kefir. In our study, it was observed that kefir had a protective and curative effect on CTX-induced toxicity and oxidative stress and could be a strong protector.