Determination of fatty acid compositions of total lipid, phospholipid and triacylglycerol fractions of the wild edible mushroom Pleurotus ostreatus and Russula delica with cytotoxic activities on pc-3 cell lines

Abstract

Fatty acids (FAs) of Pleurotus ostreatus and Russula delica were identified in TL (Total Lipid), TG (Triacylglycerol ) and PL (phospholipid) fractions. The major FAs of TL, TG, PL in both species were Cl6:0 (palmitic açid, PA), Cl8:1 n-9 (oleic acid, OLA) and Cl8:2 n-6 (linoleic acid, LA). In both species, total PUFA amounts were found to be higher than total MUFA and total SFA in TL, TG and PL fractions. The efficient production of the fatty acids especially linolenic and oleic acids which are majorly needed in building blocks of dietary human has confirmed these species as good source of nutrition. On the other hand, insufficiently studied cytotoxic activity (using pC-3 cell lines) of these mushrooms were investigated by using various solvent systems Ethyl acetate extract of Russula delica and, Pleurltus ostreatus showed significant inhibitory value at the concentrations of 520-530 µg/ml (99,45%- 92,82%) against PC-3 cell lines with IC50; 274,53-297,77 µg /mL respectively. Methanol extracts did not show any cytotoxic activity. lt has been found that the potential of cytotoxic activity is depended on concentration and solvent type of extracts. As a result, the present study is a guide for biochemiçal and nutritional values of the both species and can be useful for further investigation on plıarnıacological applications.