An Endo-1,5-.ALPHA.-L-Arabinase, Which Can Disintegrate Potato Tissue.

Abstract

An endo-1, 5-α-L-arabinase [EC 3.2.1.99] has been highly purified from the culture fluid of Bacillus subtilis IFO 3022 grown on a medium containing beet pulp extract. The molecular weight of the enzyme was 33, 000 by SDS polyacrylamide gel electrophoresis. The purified enzyme was active on 1, 5-arabinan, and inactive on p-nitrophenyl α-L-arabinofuranoside. The optimum pH was 6.0. The enzyme was inhibited by Hg2+, but not by L-arabonic-γ-lactone. The purified enzyme was able to loosen potato tissue.