Nephroprotective Effects of Visnagin through Modulation of Macrophage Polarization, Oxidative Stress, Inflammation and Apoptosis in Renal I/R Injury

Pekince-Ozoner, MerveSeker, UgurKaya, SevalYuksel, MeralDemir, MehmetSagir, SuleymanGokdemir, Gul Sahika2026-04-162026-04-1620262391-5463https://hdl.handle.net/20.500.12514/10776https://doi.org/10.1515/med-2026-1399Objectives: The study aimed to investigate the nephroprotective effects of visnagin on renal ischemia-reperfusion (I/R) injury and the role of M1/M2 macrophage polarization in this process. Methods: Forty-two adult rats were divided into six groups: Control, Visnagin30 mg/kg, Visnagin60 mg/kg, I/R, I/R + Visnagin30 mg/kg, I/R + Visnagin60 mg/kg (n=7). Bilateral renal ischemia was induced by clamping for 25 min, followed by 2 h of reperfusion. Visnagin or vehicle was administered to the animals intraperitoneally 2 h before reperfusion. At the end of the study, kidney samples were collected for analysis of oxidative stress, inflammatory cytokines, apoptotic protein expression, and M1/M2 macrophage polarization. Results: I/R injury increased malondialdehyde (MDA), chemiluminescence (CL), IL-1 beta, and IL-6 levels while decreasing glutathione (GSH) in renal tissue, indicating enhanced oxidative stress (p<0.001) and inflammation (p<0.05). Histopathological examination showed glomerular atrophy, tubular degeneration, and intertubular hemorrhage. Visnagin treatment at 60 mg/kg significantly reduced MDA, CL, and IL-1 beta levels, and increased GSH (p<0.05). Immunohistochemically, visnagin decreased Bax (p<0.001), caspase-3 (p<0.01), and TNF-alpha (p<0.01) expressions elevated by I/R injury. Furthermore, visnagin reversed I/R induced M1/M2 macrophage polarization (CD86 up arrow, CD163 down arrow), decreasing CD86 (p<0.05) and increasing CD163 immunodensity (p<0.05). Conclusions: Visnagin treatment (60 mg/kg) exerts promising nephroprotective effects in renal I/R injury by reducing oxidative stress, inflammation, apoptosis, and modulating M1/M2 macrophage polarization.en10.1515/med-2026-1399info:eu-repo/semantics/openAccessIschemia-Reperfusion InjuryApoptosisMacrophage PolarizationVisnaginInflammationOxidative StressNephroprotective Effects of Visnagin through Modulation of Macrophage Polarization, Oxidative Stress, Inflammation and Apoptosis in Renal I/R InjuryArticle2-s2.0-105032717773