PubMed İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/3597

Browse

Now showing 1 - 3 of 3

Anticancer, Antioxidant and Antimicrobial Activity of Elaeagnus Angustifolia L. Leaf Extract
(Dove Medical Press Ltd, 2025) Do Gan, Serap; Mese, Ahmet; Baran, Mehmet Firat; Baran, Ayse; Aktepe, Necmettin; Ahmadian, Elham; Aktas, Husnu
Objective: This study was conducted to determine and compare the antioxidant, cytotoxic, and antimicrobial effects of spindle leaves of Elaeagnus angustifolia L. (E. angustifolia) (oleaster) leaves. Methods: Total phenolic content was measured using the Folin-Ciocalteu method, phenolic compound analysis by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) and antimicrobial effect by the minimum inhibition concentration (MIC) method. The free radical scavenging activity was determined by the (2,2-Diphenyl-1-picrylhydrazyl) DPPH method, the free radical scavenging activity was determined by the ABTS method, and cytotoxicity assays were performed by the MTT method in human retinal epithelium cells (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer cells (DU-145) cell lines. Results: High amounts of gallic acid, protocatechuic acid, and o-coumaric acid were identified as phenolic compounds. E. angustifolia was found to have a good antioxidant capacity and high free radical scavenging capacity. In this study, for the first time, E. angustifolia leaf extract was used to investigate cytotoxic effects on human retinal epithelium (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer (DU-145) cells and antimicrobial effects on Listeria monocytogenes American Type Culture Collection (ATTC) 7644, Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 11774, and Candida albicans ATCC 10231 microorganisms. The highest cytotoxic effect was observed in the DU-145 cell line, and the highest antimicrobial effect was observed in Listeria monocytogenes ATTC 7644 and Candida albicans ATCC 10231. The leaf extract of the plant contains some important phenolic compounds and has high free radical scavenging capacity, a good anticancer effect, and effective antimicrobial activity on yeast species such as C. albicans. Conclusion: Our study will contribute greatly to the search for anticancer and antimicrobial agents, especially from a pharmacological perspective, by examining biological activity using three different methods.
Citation - WoS: 1
Citation - Scopus: 1
Antioxidant Properties of allium Turcicum Özhatay & Cowley Plant Extract, Its Effects on the Proliferation and Migration of Cancer Cells
(Frontiers Media Sa, 2024) Ipek, Polat; Baran, Ayse; Cebe, Deniz Baris; Ahmadian, Elham; Eftekhari, Aziz; Baran, Mehmet Firat; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Cancer is a type of non-communicable disease that is responsible for numerous deaths worldwide. Cancer incidence and mortality rates are on the rise due to a combination of factors, such as a growing population, aging, and poor dietary habits. The Allium turcicum & Ouml;zhatay & Cowley plant is an endemic plant in the area where it grows and is consumed by the public due to its various benefits. This endemic plant, which generally grows in high-altitude regions, is sold in bunches because it is costly, mixed with rock salt, crushed into powder, and consumed as a spice. The cytotoxic and growth-inhibitory effects of A. turcicum & Ouml;zhatay & Cowley herb extract on human glioblastoma U373 cells, human colorectal carcinoma cell HCT-116, and healthy HUVEC cell lines were determined by the MTT method. After 24 and 48 h of application, logIC50 values in HUVEC, HCT-116, and U373 cells were defined as 3.737, 3.765; 3.513, 3.696, 4.476, and 4.104 mu g/mL, respectively. We conducted a cell migration experiment to study the A. turcicum & Ouml;zhatay & Cowley Extract (AT & Ouml;CE) impact on cancer cells' metastatic behavior. Our findings indicate that AT & Ouml;CE has an inhibitory effect on the migration potential of the cells used in the study. We conducted experiments using DPPH, ABTS, CUPRAC, and total phenolic content to assess the antioxidant properties of AT & Ouml;CE. The findings from the antioxidant activity experiments revealed an activity level of 0.20 +/- 0.046 at IC50. Additionally, the total phenolic content was measured to be 0.26 +/- 0.044 mg GAE/g.
Citation - WoS: 4
Citation - Scopus: 8
Synthesis and Characterization of Activated Carbon-Supported Magnetic Nanocomposite (mnps-Olac) Obtained From Okra Leaves as a Nanocarrier for Targeted Delivery of Morin Hydrate
(Frontiers Media Sa, 2024) Ozic, Cem; Ertas, Erdal; Baran, Mehmet Firat; Baran, Ayse; Ahmadian, Elham; Eftekhari, Aziz; Yildiztekin, Mahmut; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Introduction The method of encapsulating the drug molecule in a carrier, such as a magnetic nanoparticle, is a promising development that has the potential to deliver the medicine to the site where it is intended to be administered. Morin is a pentahydroxyflavone obtained from the leaves, stems, and fruits of various plantsmainly from the Moraceae family exhibiting diverse pharmacological activities such as anti-inflammatory, anti-oxidant, and free radical scavenging and helps treat diseases such as diabetes, myocardial infarction and cancer.Methods In this study, we conducted the synthesis of a nanocomposite with magnetic properties by coating biocompatible activated carbon obtained from okra plant leaves with magnetic nanoparticles.Results Characterization of the synthesized activated carbon-coated magnetic nanocomposite was confirmed by Fourier transform infrared, scanning electron microscopy, dynamic light scattering, and zeta potential. The cytotoxic effects of the drug-loaded magnetic nanocomposite were examined in HT-29 (Colorectal), MCF-7 (breast), U373 (brain), T98-G (Glioblastoma) cancer cell lines, and human umbilical vein endothelial cells healthy cell line.Discussion We studied the loading and release behavior of morin hydrate in the activated carbon-coated magnetic nanocomposite. Activated carbon-coated magnetic nanocomposite carriers can show promising results for the delivery of Morin hydrate drugs to the targeted site.

Browse

Browsing PubMed İndeksli Yayınlar Koleksiyonu by Author "Ahmadian, Elham"