PubMed İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/3597

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Attitudes of Health Sciences Students in Turkey Towards Individuals With Different Sexual Orientations
(Womens Health & Action Research Centre, 2025) Deger, Vasfiye B.; Kacan, Havva
Study was planned in a descriptive design to examine how the empathic tendencies of students in the Faculty of Health Sciences affect their attitudes toward individuals who have different sexual orientations and was conducted with seven hundred and six students. The data collected online using the Student Information Form, Empathic Tendency Scale (ETS) and Attitudes Toward Lesbian and Gay Men Scale (ATLGS). Participants' mean scores were 62.98 +/- 18.79 on the ESS and 29.02 +/- 10.75 on the ATLGS. When the correlation between ETS and ATLGS scores was analyzed, a positive and moderate (p<0.05) correlation (r = 0.62) was found between ETS and ATLGS scores. These scores were moderate and the correlation between ETS and ATLGS scores was significant (F=438.585; p<0.05). Empathic disposition explained 38.3% of the total change in ATLGS level (R2=0.383) and increased ATLGS level (B=0.620). It was found that as students' empathic skills improved, their homophobic attitudes decreased.
Gynaecological Cancer Awareness and Healthy Lifestyle Behaviors of Women Aged 20-65 Years: a Descriptive Cross-Sectional Study
(Womens Health & Action Research Centre, 2025) Yasar, Beril Nisa; Seker, Sevda Arslan
For both individual and community health, women's health is vital. In particular, gynecological cancers can be prevented or treated by adopting healthy lifestyles, raising awareness, and detecting them early. This study aimed to identify levels of gynecological cancer awareness and healthy lifestyle behaviors among women aged 20-65 years. There were 251 women in the descriptive cross-sectional study. The Gynecological Cancers Awareness Scale (GCAS), the Descriptive Characteristics Form, and the Healthy Lifestyle Behavior Scale-II (HLBS-II) were used to gather data. The data was analyzed using the Pearson's correlation test, multiple linear regression, and descriptive statistics. The average HLBS-II score was 123.53 +/- 20.75, while the average GCAS score was 149.64 +/- 21.30. The HLBS-II and GCAS scores showed a statistically significant positive correlation. Women knowledgeable about early diagnosis methods scored 10.758 times higher on the GCAS, while women familiar with vulvar self-examination scored 11.016 times higher. Employed women had a mean HLBS-II score 6.124 times higher than non-employed women (p<0.05). Women's awareness of gynecological cancer was high, but they also had moderately good lifestyle choices. To raise awareness and promote healthy lifestyle choices, healthcare professionals are advised to take part in health-promoting initiatives.
The Performance of the Naples Prognostic Score in Predicting One-Year Mortality and Major Adverse Cardiovascular Events After Transcatheter Aortic Valve Implantation in Patients With Severe Aortic Stenosis
(Polish Cardiac Soc, 2025) Gitmez, Mesut; Guzel, Tuncay; Kis, Mehmet; Coskun, Ferhat; Isik, Mehmet Ali; Aktan, Adem; Ertas, Faruk
Background: Existing risk scores for transcatheter aortic valve implantation (TAVI) may not fully capture patient complexity. Combining nutritional and inflammatory markers, the NPS (the NAPLES prognostic score) might improve outcome prediction. Aims: This study investigated the associations of the NPS with one-year mortality and major adverse cardiovascular events (MACEs) in TAVI patients. Material and methods:This retrospective analysis included 222 patients with severe aortic stenosis who underwent TAVI. The NPS was calculated based on the serum alb & uuml;min concentration, cholesterol concentration, lymphocyte/monocyte ratio, and neutrophil/lymphocyte ratio. The patients were subsequently categorized into two groups: the low-NPS group (NPS 0-2) and the high-NPS group (NPS 3-4). Results: A high NPS was significantly associated with increased one-year mortality (4.8% vs. 23.7%; P <0.001) and MACE rates (7.2% vs. 35.9%; P <0.001). Cox regression analysis demonstrated that a high NPS was an independent predictor of both mortality (HR, 5.94; 95% CI, 2.03-17.37; P = 0.001) and MACEs (HR, 5.09; 95% CI, 2.15-12.02; P <0.001). Conclusions: The NPS emerged as a potential predictor of long-term mortality and MACEs in TAVI patients. Further validation through larger, multicenter, studies is warranted.This research contributes valuable data on the role of the NPS in TAVI risk stratification.
Green Synthesis and Characterization of Silver Nanoparticles Using Anchusa Officinalis: Antimicrobial and Cytotoxic Potential
(Dove Medical Press Ltd, 2025) Keskin, Cumali; Aslan, Seyhan; Baran, Mehmet Firat; Baran, Ayse; Eftekhari, Aziz; Adican, Mehmet Tevfik; Mohamed, Ali Jimale
Objective: Anchusa officinalis L. (A. officinalis) is a herbaceous traditional medicinal plant used in the treatment of some diseases. The presence of its medicinal properties suggested that A. officinalis (AO) leaf extract could be used as a coating agent for the environmentally friendly production of silver nanoparticles (AgNPs). Methods: The synthesized biogenic silver nanoparticles (AO-AgNPs) were characterized using different techniques. The antimicrobial activity of AgNPs against common bacterial pathogenic strains was determined by the minimum inhibitory concentration (MIC) method. The presence of phytochemicals was determined by LSMS/MS. The MTT assay was used to investigate AO-AgNPs' cytotoxic activity in malignant (LnCap, Caco2, MDA-MB2, A549) and healthy (HEK-293) cell lines. Results: LC-MS/MS analysis detected the presence of rich phytochemicals that may be responsible for reduction reactions. Biogenic AO-AgNPs exhibited effective inhibition of the growth of pathogenic microorganisms at low concentrations. The most effective antimicrobial activity was measured as 0.5 mu g/mL MIC against S. aureus, E. coli, and C. albicans. Moreover, AO-AgNPs showed significant inhibition on the growth of cancerous cell lines, especially at a concentration of 25 mu g/mL. On the contrary, it was determined that the inhibition rate decreased in the growth of healthy cell lines due to the increase in concentration. The lowest EC50 values were determined as 15.15 mu g/mL in A549 cells. Conclusion: The obtained results showed that AO could be an important source for the synthesis of AgNPs. Especially their ability to inhibit the growth of antibiotic-resistant pathogenic bacteria at low concentrations compared to common antibiotics indicates that AOAgNPs can be used as biomedical agents in various areas. Moreover, their suppressive effect on cancerous cell lines showed that they have the potential to be used as an anticancer agent, but due to their proliferative effect on healthy cell lines, care should be taken in determining the appropriate dose.
Low-Intensity Extracorporeal Shock Wave Therapy and Platelet-Rich Plasma: Effective Combination Treatment of Chronic-Phase Peyronie's Disease
(Iniestares, S.a., 2025) Ergun, Muslum; Sagir, Suleyman
Objective: This study aimed to investigate the effectiveness, safety and outcomes of the combination therapy of low-intensity extracorporeal shockwave therapy (Li-ESWT) and platelet-rich plasma (PRP) for the treatment of chronic-phase Peyronie's Methods: The clinical outcomes of patients diagnosed with Peyronie's disease and treated with Li-ESWT combined with PRP at our clinic between January 2018 and January 2024 were retrospectively reviewed and analysed. Twenty-three patients were excluded based on inclusion criteria, leaving 26 patients for the retrospective analysis. Each patient received three sessions of PRP and six sessions of Li-ESWT. The treatment regimen involved sessions administered twice weekly over a period of 3 weeks, followed by 1-week post-treatment follow-up. The patients were then monitored for 24 weeks. Result: After treatment, no significant reduction in average plaque size was observed. However, a statistically significant average improvement of 10 degrees was noted for penile curvature. Among the patients, 14 (53.8%) reported satisfaction with the treatment outcome, and 12 (46.2%) expressed dissatisfaction. No significant adverse effects were observed at the injection sites or in the areas subjected to Li-ESWT post-procedure. Conclusions: The combination of Li-ESWT and PRP is effective and safe for the treatment of chronic-phase Peyronie's disease.
The Predictive Value of the Cha2ds2-Vasc Score in the Development of Contrast-Induced Nephropathy After Endovascular Intervention in Peripheral Artery Disease
(Elsevier Science inc, 2025) Evsen, Ali; Aktan, Adem; Kilic, Raif; Isik, Mehmet Ali; Yalcin, Abdulaziz; Guzel, Tuncay; Ozbek, Mehmet
Background: Contrast-induced nephropathy (CIN) is a frequent complication of endovascular interventions for peripheral artery disease (PAD). It is linked to renal dysfunction, extended hospital stays, increased cardiovascular events, and higher mortality rates. The CHA2DS2-VASc score, widely utilized for assessing cardioembolic risk and guiding anticoagulation therapy in nonvalvular atrial fibrillation, encompasses risk factors that overlap with those of CIN. This study investigates whether the CHA2DS2-VASc score can predict CIN in PAD patients undergoing endovascular interventions. Methods: The study included 754 consecutive PAD patients who underwent endovascular procedures at 2 centers. Each patient's CHA2DS2-VASc score was calculated and categorized into low (<3) and high (>= 3) groups. Patients were retrospectively monitored for CIN development and divided into CIN-positive (CIN+) and CIN-negative (CIN-) groups. Univariate and multivariate regression analyses were performed to identify independent predictors of CIN, and a significance level of P < 0.05 was used for all statistical analyses. Results: Of the 754 patients, 178 (23.6%) developed CIN, with 151 (84.8%) occurring in the high CHA2DS2-VASc score group (P < 0.001). The CHA2DS2-VASc score was significantly higher in the CIN(+) group compared to the CIN(-) group (P < 0.001). Regression analysis identified the CHA2DS2-VASc score (odds ratio [OR]: 1.574, 95% confidence interval [CI]: 1.2981.907, P < 0.001), baseline creatinine (OR: 2.296, 95% CI: 1.580-3.335, P < 0.001), and hemoglobin (OR: 0.915, 95% CI: 0.844-0.992, P < 0.001) as independent risk factors. A CHA2DS2-VASc score cutoff of 2.5 predicted CIN with 85% sensitivity and 42% specificity. Conclusion: The CHA2DS2-VASc score is an independent predictor of the development of CIN in patients with PAD undergoing endovascular intervention.
Myricetin Promotes Migration and Prevents Palmitate-Induced Apoptosis in Cultured Tenocytes Through Ampk-Dependent Pathways
(Academic Press inc Elsevier Science, 2025) Lim, Do Su; Ahn, Sung Ho; Cho, Wonjun; Gwon, Hyeon Ji; Ko, Jun Hwi; Pyo, Min Kyung; Jung, Tae Woo
Myricetin (Myr), a flavonoid present in vegetables and fruits, has been shown to ameliorate inflammation and oxidative stress in various disease models. However, the effects of Myr on hyperlipidemic tenocytes have not been studied. Herein, we aimed to investigate the effects of Myr on the features of tendinopathy in cultured tenocytes under hyperlipidemic conditions. Reactive oxygen species (ROS) were detected by DCFDA. Hydrogen peroxide (H2O2), malondialdehyde (MDA), and caspase 3 activity were quantified via matched assay kits. Apoptotic cells were detected via TUNEL staining. Proteins investigated in this study were evaluated through Western blotting. Treatment with Myr enhanced tenocyte migration and prevented apoptosis, inflammation and oxidative stress in palmitate-treated tenocytes. Myr treatment increased the phosphorylation of AMPK, and the expression of PGC1 alpha and FGF2. siRNA targeting AMPK abrogated the effects of Myr on palmitate-treated tenocytes. However, FGF2 siRNA reduced the impacts of Myr on only cell migration and ECM signaling. These in vitro results suggest that Myr promotes tenocyte migration and ECM signaling via AMPK/FGF2 signaling and attenuates apoptosis through the AMPK-mediated suppression of inflammation and oxidative stress in hyperlipidemic tenocytes. This study sheds light on therapeutic strategies for treating obesity-related tendinopathy.
Traditional Care Practices Known And/Or Used by Different Ethnic Groups for Newborns During the Postpartum Period
(BMC, 2025) Deger, Vasfiye Bayram; Butun, Ahmet
BackgroundTraditional care practices play a significant role in shaping care provided to newborns across different ethnic groups. Understanding traditional care practices is crucial for integrating culturally sensitive approaches into modern healthcare systems. This study aims to fill the gap in the literature regarding the different traditional care practices across ethnic groups. The aim of this study was to determine the traditional beliefs and practices of women from different ethnic groups living in Mardin regarding newborn care.MethodsThis study is a descriptive cross-sectional study. The sample consisted of women from four ethnic groups who volunteered to participate in the study with at least one child living in Mardin, Turkey. Snowball and convenience sampling methods were used to recruit participants. The study was completed with 188 Assyrian, 197 Turkish, 181 Arab, and 175 Kurdish women. In total, 741 women participated in this study. Data were collected between 18 December 2023 and 14 June 2024. The data were collected face-to-face using a questionnaire. The data were analysed using SPSS for Windows 22.0.ResultsThis study identified significant differences in traditional care practices across four ethnic groups (Assyrian, Turkish, Arab, and Kurdish). Traditional care practices regarding umbilical cord care, swaddling the baby, alleviating gas pain, preventing neonatal jaundice, care for canker sores, and alleviating diaper rash were identified. Assyrian participants were more likely to apply salt to the umbilical cord (14.9%), while Arab participants rarely used this practice (0.6%). Traditional practices for relieving gas pain included drinking herbal teas such as anise, fennel, linden, and cumin. Rubbing and rubbing breast milk on the abdomen, patting on the back, and massaging were other practices for alleviating gas pain. Arab participants were more likely to use anise tea to alleviate gas pain (75.1%), while Kurdish participants preferred fennel tea (22.3%). Practices regarding alleviating diaper rash included applying saturated fat, olive oil, and powder. Arab participants were more likely to apply olive oil for diaper rash (45.9%), while Assyrian participants commonly used dry earth ('H & ouml;ll & uuml;k'). These results highlight the cultural diversity in newborn care practices and underscore the importance of culturally sensitive healthcare interventions.ConclusionThis study highlighted the significance of traditional care practices in newborn care across four ethnic groups. There are many differences between ethnic groups regarding traditional care practices during the postpartum period. Understanding such differences is crucial for developing culturally sensitive interventions that support maternal well-being and enhance health outcomes for both mothers and infants.
The Role of Mid-Trimester Bun and Creatinine Assessment in Predicting Preeclampsia: Retrospective Case-Control Study
(Mdpi, 2025) Kavak, Ebru Celik; Akcabay, Cigdem; Demircan, Meryem; Batmaz, Ibrahim; Sanli, Cengiz; Senocak, Ahmet; Kavak, Salih Burcin
Background and Objectives: Preeclampsia (PE) is a major cause of adverse perinatal outcomes. Early diagnosis of pregnant women at risk of PE can facilitate disease prevention and management. However, the presence of different phenotypes of the disease complicates its prediction. In particular, the challenges in the early diagnosis of term PE cases necessitate research on PE prediction in the second and third trimesters. This study aims to examine the association between PE development and mid-trimester blood urea nitrogen (BUN), serum creatinine, and the BUN/creatinine ratio in pregnant women. Materials and Methods: This retrospective case-control study was conducted on women diagnosed with PE. Pregnant women who underwent routine biochemical blood tests between the 18th and 24th weeks of gestation and subsequently gave birth at our hospital between January 2022 and May 2023 were categorized into three groups. Accordingly, healthy women who had term deliveries were classified as Group 1 (150 cases), women diagnosed with PE were classified as Group 2 (58 cases), and those diagnosed with severe PE were classified as Group 3 (44 cases). Results: There were no significant differences in age, gravidity, parity, body mass index, or gestational week at blood sampling between the patient and control groups (p > 0.05). When comparing the mean blood urea nitrogen, serum creatinine, and BUN/creatinine ratios, a significant difference was observed between the control group and those who developed PE (p = 0.001, p < 0.001, and p = 0.031, respectively). Univariate analysis revealed a significant association between BUN levels and PE development (OR 1.083; 95% CI, 1.031-1.139; p = 0.002). A stronger association was observed between serum creatinine levels and PE development (OR 112.344; 95% CI, 11.649-1083.416; p < 0.001). However, no significant association was found between the BUN/creatinine ratio and PE in univariate analysis (OR 1.003; 95% CI, 0.979-1.028; p > 0.05). Mid-trimester BUN and serum creatinine levels were significantly higher in patients who developed PE and severe PE. The AUC value for the BUN parameter in predicting PE was 0.614 (AUC 0.614; 95% CI, 0.539-0.689; p = 0.002). A BUN cut-off value of 16.2 mg/dL predicted disease development with a sensitivity of 52.9% and a specificity of 74%. Similarly, the AUC value for the serum creatinine parameter in predicting PE was 0.644 (AUC 0.644; 95% CI, 0.574-0.751; p < 0.001). A serum creatinine cut-off value of 0.58 mg/dL was able to predict disease development with 37.2% sensitivity and 88% specificity. No significant AUC value was obtained for the BUN/creatinine ratio (p > 0.05). Conclusions: Our findings indicate that elevated BUN and serum creatinine levels measured during the mid-trimester (18-24 weeks) are associated with an increased risk of developing preeclampsia.
Evaluation of Thiol/Disulfide Homeostasis and Ischemia Modified Albumin as Potential Markers for Periodontitis
(BMC, 2025) Karci, Bilge; Savas, Hasan Basri
Background The current study aimed to assess the impact of periodontitis on oxidative stress parameters by examining serum total antioxidant capacity (TAS), total oxidant status (TOS), oxidative stress index (OSI), thiol/ disulfide homeostasis and ischemia modified albumin (IMA). Methods The study had 90 participants, categorized into 3 groups: Group 1: Periodontally healthy; Group 2: Stage II Grade B periodontitis; Group 3: Stage III and IV Grade B periodontitis. Demographic and periodontal variables were assessed. The levels of serum TAS, TOS, OSI, IMA, and thiol/disulfide were assessed. Results No significant differences in sex and age were detected among the groups (p > 0.05). When compared to Group 1, all clinical measurements were statistically significantly greater in Group 3 (p < 0.05). Statistical analysis revealed no significant differences in serum TAS, TOS, and OSI levels among the groups (p > 0.05). The highest serum IMA value was observed in Group 3 (p = 0.037), whereas native thiol (p = 0.00), total thiol (p = 0.00) and disulfide values (p = 0.023) were highest in Group 1. Conclusions These findings indicate that thiol/disulfide homeostasis and IMA could hold promise as a potential biomarker of inflammation in periodontitis.
Effects of Trimetazidine on Oxidant-Antioxidant Balance and Angiogenesis; an in Vivo Experimental Study
(BMC, 2025) Kayan, Fethullah; Savas, Hasan Basri
Background We evaluated the effects of trimetazidine (TMZ) on the oxidative-antioxidative balance and angiogenesis in an in vivo experimental model. This study aims to evaluate the effects of trimetazidine on angiogenesis through histological analysis and to assess its impact on oxidative-antioxidative balance through biochemical measurements. Methods In this study, Ross 308 breed chicken eggs (n = 40) were used, and embryos were divided into four distinct groups. On the eighth day of incubation, the vascular density of the embryos was examined. Following the assessment of vascular development, 4-5 mL of albumin was collected via syringe to measure oxidative stress markers. Each group consisted of 10 embryos, with a total of 40 embryos used in the study. The groups were organized as follows: Control Group (CG), Bevacizumab Group (BC), Trimetazidine 10(-)(4) Group, and Trimetazidine 10(-)(5) Group. Results When the total oxidative capacity (TOC) levels were compared among the groups, the bevacizumab group exhibited significantly higher values than the control group (p < 0.05). In oxidative stress index (OSI) measurements, the bevacizumab group also showed significantly higher values compared to the control group (p < 0.05). In contrast, when the total antioxidant capacity (TAC) levels were compared, both the Trimetazidine 10(-)(4) and Trimetazidine 10(-)(5) groups demonstrated significantly higher values than the control group (p < 0.05). Regarding angiogenesis scoring, the bevacizumab group exhibited a significant anti-angiogenic effect compared to the control group. However, no statistically significant difference was observed between the Trimetazidine 10(-)(4) and Trimetazidine 10(-)(5) groups and the control group (p > 0.05). Conclusion Trimetazidine demonstrated significant antioxidant activity in an in vivo Chorioallantoic Membrane (CAM) model at both 10(-)(4) M and 10(-)(5) M concentrations. However, no positive or negative effects on angiogenesis were detected. We believe that the real-time observation of angiogenesis in our study provided significant value to our research.
Long-Term Impact of Self-Mobilization Via Telerehabilitation Vs. Manual Therapy and Home Exercise on Pain and Function in Cervical Degenerative Disease
(Taylor & Francis Ltd, 2025) Aygul, Gokhan; Tuncer, Aysenur; Ozaltin, Gulfem Ezgi; Celik, Erman Berk; Aygul, Bedriye Ilkay
Purpose: Cervical Degenerative Disease (CDD) commonly leads to neck pain, functional impairment, and reduced quality of life. This study aimed to compare the long-term effects of home exercise, manual therapy, and telerehabilitation-assisted treatment on pain, functionality, and patient satisfaction in individuals with CDD. Patients and methods: Sixty-six patients diagnosed with CDD were randomly assigned to three groups: home exercise (n = 23), manual therapy (n = 22), and telerehabilitation (n = 21). All groups participated in an 8-week exercise program, with the manual therapy and telerehabilitation groups receiving additional sessions twice a week. Pain was measured using the Visual Analogue Scale (VAS), pain threshold with an algometer, neck function with the Neck Disability Index and range of motion (ROM), and patient satisfaction with the Patient Satisfaction Questionnaire-18. Results: All groups significantly improved pain, function, and ROM over time (p < 0.05). But there were no significant differences between groups at the 6-month follow-up. Manual therapy and telerehabilitation significantly enhanced patient satisfaction, particularly in communication and technical quality (p < 0.05). Conclusion: Home exercise, manual therapy, and telerehabilitation improve long-term outcomes in CDD. Manual therapy and telerehabilitation provide greater patient satisfaction, making them viable options for long-term management. Telerehabilitation can be used as an alternative when necessary.
Serum Selenium Levels and Subacute Thyroiditis: Associations With Disease Course and Long-Term Outcomes in a Case-Control Study
(BMC, 2025) Sakiz, Davut; Calapkulu, Murat; Sencar, Muhammed Erkam; Unsal, Ilknur Ozturk; Hepsen, Sema; Bostan, Hayri; Cakal, Erman
BackgroundSubacute thyroiditis (SAT) is an inflammatory disease that induces thyrotoxicosis. Selenium is an essential trace element in thyroid physiology, which has anti-inflammatory and antioxidant properties. However, the relationship between serum selenium levels and SAT has not been well studied. The objective of this study was to evaluate serum selenium levels in patients with SAT compared to healthy controls and to investigate potential correlations between selenium status and clinical outcomes, including disease severity, delayed remission, recurrence, and the development of permanent hypothyroidism.MethodsThis case-control study included 59 patients with SAT and 50 healthy control subjects. Serum selenium levels were analysed using inductively coupled plasma mass spectrometry.ResultsThe serum selenium levels of patients with SAT were 69.10 (24.60-130.20) mu g/L, while those of the control group were 64.20 (39.21-106.80) mu g/L (p = 0.121). A negative correlation was detected between serum selenium levels and erythrocyte sedimentation rate, C-reactive protein, neutrophil-to-lymphocyte ratio, free thyroxine, and pain severity. Serum selenium levels did not significantly differ in terms of the response to initial treatment, recurrence, and permanent hypothyroidism.ConclusionThe study results showed no significant difference in serum selenium levels between patients with SAT and the control group. These results suggest that although lower serum selenium levels may be associated with a more severe and painful SAT course, there is no impact on the long-term prognosis.
Evaluation of Children and Adolescents With Thalassemia Major in Terms of Osteoporosis: a Single-Centre Experience
(Mdpi, 2025) Demir, Hasan; Talay, Mehmet Nur; Talay, Mehmet Nur; Ozgun, Nezir; Ozbek, Mehmet Nuri
Background/Objectives: This study aimed to determine the frequency of osteoporosis in children and adolescents with thalassemia major (TM) and to identify risk factors for the early development of osteoporosis. Methods: This retrospective study included 27 patients under 18 years of age receiving regular blood transfusions and chelation therapy for TM at our hospital. Bone mineral density (BMD) was measured by dual-energy x-ray absorptiometry, and a lumbar spine Z-score <-2 was considered osteoporotic. Patients with osteoporosis were classified as Group 1 and those without osteoporosis as Group 2. Results: Osteoporosis was detected in 22.2% of the study population. The mean age was 13.83 +/- 2.85 years in Group 1 and 7.95 +/- 5.05 years in Group 2 (p = 0.012). Body weight and height were significantly lower in Group 1 (p = 0.012 and p = 0.004). Ferritin levels were 5306 +/- 1506 ng/mL in Group 1 and 2020 +/- 1205 ng/mL in Group 2, and the difference was significant (p = 0.001). Group 1 had significantly lower Ca and P levels (p < 0.001, p = 0.038). BMD was negatively correlated with ferritin (r = -0.791, p < 0.001) and positively correlated with calcium (r = 0.499, p = 0.008). Conclusions: Osteoporosis is a common condition in TM patients. Patients with risk factors should be followed more closely. These patients should be identified before BMD decreases. To prevent osteoporosis, regular BMD scans should be performed, calcium and vitamin D supplementation should be provided, and physical activity should be encouraged.
Comparison of Pde-5 Inhibitors Used in Erectile Dysfunction With Some Candidate Molecules: a Study Involving Molecular Docking, Admet, Dft, Biological Target, and Activity
(Bmc, 2025) Sağır, Süleyman; Unsal, Velid; Oner, Erkan; Yildiz, Resit; Mert, Basak Dogru
Erectile dysfunction (ED) is a urological condition defined as the inability of a man to achieve or maintain an erection. This condition negatively affects his sexual performance and the performance of his partner. Phosphodiesterase type 5 (PDE5) inhibitors are commonly used to treat ED. Arginase II plays an important role in regulating L-arginine to NO synthase in the smooth muscle of the human corpus cavernosum of the penis. NO is a molecule essential for regulating a variety of functions, including arterial blood pressure, penile erection, and energy balance. Substances such as vardenafil, alprostadil, papaverine, and resveratrol increase NO production, thereby supporting sexual function and vascular health. Additionally, NO donors such as L-arginine, L-citrulline, and alpha-lipoic acid provide effective alternatives when used in combination with PDE5 inhibitors. Medications used in the treatment of ED include vardenafil, alprostadil, and papaverine. In addition, although molecules such as L-arginine, citrulline, resveratrol, alpha-lipoic acid, and rutin are thought to play a role in ED, their pharmacological and molecular effects have not been sufficiently elucidated. The aim of this study was to investigate the effects of these molecules in the treatment of ED by computer-based calculations, to obtain new information about them and to inspire new treatment strategies for ED. The physicochemical, molecular and pharmacokinetic properties of the compounds were determined by SwissADME software, and ADMET (absorption, distribution, metabolism, excretion and toxicity) data were determined by ADMETlab 3.0 software. Biological target and activity data were obtained by MolPredictX and PASS Online software. While the Gaussian 09 program was used for DFT calculations, PyMOL, AutodockTools 4.2.6, AutoDock Vina, and Biovia Discovery programs were used for molecular docking studies. It was found that L-arginine, citrulline, resveratrol and alpha-lipoic acid were well absorbed from the intestine, while rutin showed limited absorption. When their metabolic risks were evaluated, L-arginine and citrulline were found to have lower toxicity. Molecular docking results of rutin and resveratrol were remarkable. The electronic properties of the compounds were explained by DFT calculations. L-arginine and citrulline were found to have low toxicity and positive therapeutic effects. L-arginine and citrulline stand out as promising candidates for future research. Although resveratrol data are promising, unfortunately their potential toxicity and metabolic interactions require further investigation. It is important to learn more about these compounds or conduct research to improve their therapeutic efficacy. Although computer-based calculations play an important role in toxicity predictions, drug interactions, pharmacokinetics and toxicity properties should be carefully evaluated.
Antibiotic Resistance and Mortality in Icu Patients: a Retrospective Analysis of First Culture Growth Results
(Mdpi, 2025) Kilinc, Metin
Objectives: This study aimed to analyze the antibiotic resistance patterns of microorganisms isolated from intensive care unit (ICU) patients and evaluate their impact on mortality and length of ICU stay. Given the increasing prevalence of multidrug-resistant (MDR) pathogens in critically ill patients, understanding their resistance profiles is crucial for optimizing empirical antibiotic therapy and improving patient outcomes. Methods: This retrospective study included 237 ICU patients admitted between 1 July 2022, and 1 January 2024. The initial culture growth results from blood and urine samples were analyzed. Microorganism identification was performed using VITEK 2 Compact and conventional bacteriological methods, while antibiotic susceptibility testing followed CLSI 2022 and EUCAST 2022 guidelines. Results: A total of 237 ICU patients were included in this study. The most frequently isolated microorganisms were Escherichia coli (E. coli) (44.3%), Klebsiella pneumoniae (K. pneumoniae) (35.0%), and Pseudomonas aeruginosa (P. aeruginosa) (25.3%), with Acinetobacter baumannii (A. baumannii) (31.2%) being the most resistant pathogen. Among Gram-positive bacteria, methicillin-resistant Staphylococcus aureus (MRSA) (12.2%) and vancomycin-resistant enterococci (VRE) (21.5%) were the most frequently identified multidrug-resistant (MDR) pathogens. Regarding antimicrobial resistance, carbapenem resistance was highest in A. baumannii (55%), followed by P. aeruginosa (40%) and K. pneumoniae (30%). Additionally, ESBL-producing E. coli (43.2%) and K. pneumoniae (38.5%), as well as carbapenemase-producing K. pneumoniae (18.6%) and E. coli (9.2%), were identified as key resistance mechanisms impacting clinical outcomes. Patients with MDR infections had significantly longer ICU stays (p < 0.05) and higher mortality rates. The Kaplan-Meier survival analysis revealed that A. baumannii infections were associated with the highest mortality risk (HR: 4.6, p < 0.001), followed by MRSA (HR: 3.5, p = 0.005) and P. aeruginosa (HR: 2.8, p = 0.01). Among laboratory biomarkers, elevated procalcitonin (>= 2 ng/mL, OR: 2.8, p = 0.008) and CRP (>= 100 mg/L, OR: 2.2, p = 0.01) were significantly associated with ICU mortality. Additionally, patients who remained in the ICU for more than seven days had a 1.4-fold increased risk of mortality (p = 0.02), further emphasizing the impact of prolonged hospitalization on adverse outcomes. Conclusions: MDR pathogens, particularly A. baumannii, MRSA, P. aeruginosa, and K. pneumoniae, are associated with longer ICU stays and higher mortality rates. Carbapenem, cephalosporin, fluoroquinolone, and aminoglycoside resistance significantly impact clinical outcomes, emphasizing the urgent need for antimicrobial stewardship programs. ESBL, p-AmpC, and carbapenemase-producing Enterobacterales further worsen patient outcomes, highlighting the need for early infection control strategies and optimized empirical antibiotic selection. Biomarkers such as procalcitonin and CRP, alongside clinical severity scores, serve as valuable prognostic tools for ICU mortality.
Phytochemical Profile, Antimicrobial, Antioxidant, Anti-Xanthine Oxidase, and Anti-Elastase Activities of Centaurea Hyalolepis: an in Silico and in Vitro Analysis
(Wiley-v C H verlag Gmbh, 2025) Günbegi Çalışkan, Cemile; Caliskan, Cemile Gunbegi; Akan, Hasan
This study aimed to examine the antibacterial, antioxidant, and enzyme activity of Centaurea hyalolepis, as well as the total phenolic and carotenoid contents, and volatile and phenolic components. Gas chromatography-mass spectrometry was used for the volatile component analysis of C. hyalolepis, while liquid chromatography-mass spectrometry was used for the phenolic component analysis. Furthermore, the antimicrobial activity was evaluated using disc diffusion and microdilution techniques. The antioxidant capacity was investigated in vitro using four distinct methods (2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, cupric ion-reducing antioxidant capacity, and N,N-dimethyl-p-phenylene diamine). Additionally, both in vitro and in silico studies were carried out to determine the inhibitory effect on xanthine oxidase and elastase. Consequently, it was determined that C. hyalolepis has antioxidant properties and is a highly efficient antibacterial agent against Bacillus megaterium, Pseudomonas aeruginosa, Bacillus subtilis subsp. spizizenii, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, and Candida albicans. Additionally, C. hyalolepis contains many volatile compounds, such as 2-methyl propanoic acid, 3,5-octadien-2-one, 2,6-dimethyl cyclohexanol, hexanal, pentanal, anethole, and beta-cyclocitral. It also has a large number of phenolic components, including chlorogenic acid, apigenin 7-glucuronide, quinic acid, and 4-hydroxybenzoic acid. Moreover, C. hyalolepis was shown to inhibit xanthine oxidase and elastase.
Assessing the Prognostic Value of Halp Score in Peripheral Artery Disease: Correlation With Lesion Severity and Long-Term Mortality
(Sage Publications Ltd, 2025) Aktan, Adem; Aktan, Adem; Kilic, Raif; Yalcin, Abdulaziz; Ozbek, Mehmet
Introduction Peripheral artery disease (PAD) poses a growing clinical challenge due to an aging population, despite advances in treatment methods. Various scoring systems have emerged to predict high-risk patients, including the HALP (hemoglobin, albumin, lymphocyte, and platelet) score, known for predicting prognosis in cancers and stroke. This study assesses the HALP score's relation to lesion severity and long-term mortality in PAD patients. Methods We retrospectively analyzed 305 symptomatic PAD patients undergoing endovascular intervention. The following formula was used to calculate the HALP score: hemoglobin (g/L) x albumin (g/L) x lymphocyte count (/L) / platelet count (/L). Lesion severity was classified by TASC-II: TASC AB and TASC CD. Mortality data were obtained from hospital and social security records. Results The study involved 305 patients (mean age 64.4 +/- 11.8 years; 72.1% male), divided into survivors (208) and non-survivors (97). ROC analysis identified HALP score as the strongest predictor of long-term mortality (AUC: 0.736; 95% CI: 0.679-0.793; p < .001). HALP score (HR, 0.087; 95% CI, 0.025-1.300; p < .001), age (p < .001), DM (p = .007), and CRP (p = .013) independently predicted mortality. Kaplan-Meier analysis showed higher HALP scores linked to lower long-term mortality (Log-rank: 20.102, p < .001), with an average follow-up of 48 +/- 18 months. Conclusion The HALP score emerged as a robust predictor of PAD prognosis, surpassing individual components and other parameters. Lower HALP scores correlated with more severe lesions and reduced life expectancy.
Confounding Factors in the Tear Break-Up Time Test. Influence of Sequential Measurements
(Taylor & Francis Ltd, 2025) Acet, Yakup; Dag, Yasar
Clinical relevance The tear break-up time (BUT) test is important in diagnosing and monitoring tear film stability, and understanding potential changes in tear film dynamics during repeated measurements could improve the accuracy and reliability of the test in clinical practice. Background The study aimed to examine the changes in BUT value caused by subsequent measurements made in BUT test. Methods A total of 120 female participants were divided into groups (30 individuals each). Participants were divided into four groups based on the interval between two non-invasive tear break-up time (NI-BUT) tests: Group 1 (1-minute interval), Group 5 (5-minute interval), Group 10 (10-minute interval), and Group 15 (15-minute interval). The first NI-BUT test was called Initial NI-BUT (NI-BUT(I)), and the second test was called Subsequent NI-BUT (NI-BUT(S)). The first BUTvalue (BUT1st), the second BUT value (BUT2nd), the third BUT value (BUT3rd), the fourth BUT value (BUT4th), the fifth BUT value (BUT5th), and the average value of the first three BUTs (BUTA3) were compared between the NI-BUT(1) and NI-BUT(S) tests. Results Participant ages ranged from 18 to 36 years, with a mean of 25.1 +/- 3.2 years. The mean values of BUT1st, BUT2(nd), BUT3(rd), BUT4(th), BUT5(th), and BUTA3 in the NI-BUT(I) test were statistically significantly shorter than those in the NI-BUT(S) test for all groups (p < 0.05). The changes in the parameters were statistically significantly greater in Group 1 compared to the other groups (p < 0.05). Conclusions Subsequent measurements cause increased BUT values. The longer the interval between two measurements, the smaller the differences between the subsequent measurements. When performing the BUT test, a single measurement should be preferred if possible. If the clinician finds the results of a single measurement unsatisfactory and wishes to consider the average of multiple measurements, the interval between tests should be longer than 15 minutes.
Association of Inflammatory and Metabolic Markers With Mortality in Patients With Postoperative Femur Fractures in the Intensive Care Unit
(Mdpi, 2025) Akelma, Hakan; Celik, Enes; Demir, Ibrahim; Aydemir, Semih; Akelma, Hakan
Background and Objectives: Postoperative femur fracture in elderly patients is associated with high morbidity and mortality, especially in the intensive care unit (ICU). Various factors, including demographic and laboratory parameters, may influence mortality in this population. The aim of this study was to evaluate the association of inflammatory and metabolic markers with mortality in ICU patients with postoperative femur fractures and to identify key predictors to enhance risk stratification and improve patient outcomes. Materials and Methods: In this retrospective single-center study, we analyzed 121 patients aged over 65 years with postoperative femur fractures who were admitted to the ICU between January 2023 and January 2024. Demographic and clinical data, including comorbidities, Charlson Comorbidity Index (CCI), and Acute Physiology and Chronic Health Evaluation (APACHE II) score, were collected. Laboratory parameters such as white blood cell count (WBC), albumin, C-reactive protein (CRP), D-dimer, Pan-Immune-Inflammation Value (PIV), CRP-to-albumin ratio (CAR), neutrophil-to-lymphocyte ratio (NLR), and others were analyzed. Linear regression, logistic regression, and Receiver Operating Characteristic (ROC) analyses were performed to determine the predictive value of these markers for ICU mortality. Results: The mean age of the patients was 76.3 +/- 9.6 years, and 52.1% were female. The most common comorbidities were hypertension (67.8%) and diabetes (49.6%). ICU mortality occurred in 24 patients (20%). Significant predictors of mortality included higher CRP (>62.8 mg/L), NLR (>10.0), PIV (>450), and APACHE II scores (>23) (p < 0.001 for all). Lower albumin levels (<2.5 g/dL) were strongly associated with increased mortality (p < 0.001). ROC analysis demonstrated that the APACHE II score had the highest predictive accuracy for mortality (AUC = 0.83), followed by albumin (AUC = 0.79) and PIV (AUC = 0.76). Extended ICU stay (>10 days) was also significantly correlated with increased mortality (p < 0.001). Conclusions: This study successfully demonstrates the utility of combining traditional clinical markers, such as APACHE II score, with novel inflammatory markers, such as PIV, CAR, and NLR, in predicting mortality in ICU patients following femur fracture surgery. The integration of emerging biomarkers with well-established scoring systems offers enhanced predictive accuracy and provides valuable insights into patient management.

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