Browsing by Author "Şeker, U."

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Comparison of Different Fixatives Effects in Histochemical Stainings of Peripheral Nerve Tissue
(Cellular and Molecular Biology Association, 2024) Dörtbudak, M.B.; Demircioğlu, M.; Şeker, U.; Demircioğlu, İ.; Department of Basic Medical Sciences / Temel Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
A pathological condition in the peripheral nerve tissue, which provides the connection between the organism and the external environment, negatively affects the standard of living. The nerve tissue histotechnology is of serious importance both for scientific studies and for clinical diagnosis. The fixation, which is one of the leading procedures for histological examination of tissues, aims to preserve tissue morphology. Another essential part of the histological examination is staining process. This study, it was aimed to determine the fixative that provides optimal histological appearance in peripheral nerve tissue. Therefore, various histochemical stainings of tissues fixed with some fixatives used in practice were compared. Sciatic nerves from each rat (n=7) used in the study were fixed with different fixatives and histochemical staining was performed. In histological examination, cellular (nucleus-cytoplasm) and intercellular morphological details, staining intensity and distribution were evaluated. At the end of the study, formaldehyde was found to be the most ideal fixing agent for all stains. Although Bouin and Carnoy fixatives differed according to the staining type, their fixation quality was similar in general. Glutaraldehyde did not give as good results as other fixatives in all stainings. This study is an important technical reference for clinical and experimental studies. © 2024 Cellular and Molecular Biology Association. All rights reserved.
Histological Evaluation of Algan Hemostatic Agent's Effect in a Rat Experimental Spleen Injury Model
(Ondokuz Mayis Universitesi, 2024) Şener Akçora, D.; Mavuş, D.; Şeker, U.; Yildiz, G.; Gökçe, K.; Department of Basic Medical Sciences / Temel Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Uncontrolled hemorrhage may result from injuries to the parenchym and splenic capsule. Hemostatic material applications are among the methods used to prevent spleen parenchymal hemorrhage. Algan Hemostatic Agent (AHA) is a standardized mixture of six distinct herbs that are capable of hemostasis, either individually or in combination. Aim of this study was to investigate efficiency of AHA in bleeding control in experimental spleen injury model, and to evaluate its histopathological effects and IL-1β, TNF-α, and Bax expressions in tissue. Twenty-four Wistar albino female rats, aged 10-12 weeks, were randomly separated into three groups (n=8); control, AHA-liquid, and AHA-powder. After surgery physiological saline, liquid and powder were applied to the injured area for twenty seconds. On the tenth postoperative day all rats’ spleens were removed for histopathological and immunohistochemical analysis. The AHA-liquid group demonstrated more efficacy in controlling hemorrhage than the AHA powder group after both the initial and subsequent applications. Parenchyma of the spleen was intact, and a thin capsule was detected in the liquid group. In the powder group, thick granulation tissue was observed along with acute lymphocyte and neutrophil infiltration. Expressions of IL-1 β and TNF-α were mild in control and AHA-liquid groups and intense in AHA-powder groups. Similar Bax protein expression was detected in all groups. Current study demonstrated that liquid form of AHA was more effective in reducing local bleeding and inflammation in spleen tissue. Therefore, liquid form could be preferred in animal experiments and clinics as a rapid, safe, and effective agent for organ injury. © 2024 Ondokuz Mayis Universitesi. All rights reserved.