Browsing by Author "Baran, M. F."

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Biosynthesis and Characterization of Silver Nanoparticles Using King Oyster (pleurotus Eryngii) Extract: Effect on Some Microorganisms
(Corvinus Univ Budapest, 2019) Acay, H.; Baran, M. F.
The integration of the principles of green chemistry into nanotechnology has become one of the key issues in nanotechnology research. Metal nanoparticle production, which does not contain toxic chemicals and does not harm the environment, needs to be developed to avoid adverse effects on medical applications. In this study, Pleurotus eryngii (PE) extract was used for preparation of silver nanoparticles (AgNPs). The presence of AgNP was understood that after adding 1 mM silver nitrate (AgNO3) to the fungus extract, the reaction turned from the open yellow to reddish brown. The analysis of samples taken at different times with the UV-Visible Spectrophotometer (UV-Vis) confirms the formation of PE-AgNPs. The Scanning electron microscopy-Energy Dispersive X-Ray Spectrum (SEM-EDX) analysis showed that spherical nanoparticles were formed. X-ray crystallography (XRD), analysis is calculated from Debye-Sherers inequality, in which PE-AgNP synthesized in the study was 18.45 nm in size. It has been demonstrated by using the minimum Inhibitory Concentration (MX) method in which AgNPs have strong antimicrobial activity.
Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles (AGNPS) From Maize (Zea Mays L)
(Aloki Applied Ecological Research and Forensic inst Ltd, 2019) Eren, A.; Baran, M. F.
In recent years, the biosynthesis (green synthesis) of metal nanoparticles such as silver nanoparticles (AgNPs) have become one of the safest, most cost-effective and environmentally friendly approaches. In this study, AgNPs were synthesized using maize (Zea mays L.) leaves. For the characterization of synthesized AgNPs different techniques were used, such as X-ray diffraction spectroscopy (XRD), Ultraviolet visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), Energy dispersive X-ray spectroscopy (EDX), Fourier-transformed infrared spectroscopy (FT-IR) and Thermal gravimetric and Differential thermal analysis (TGA-DTA). The XRD results showed that AgNPs had a mean diameter of 12.63 nm and a crystal-like appearance. In addition, antimicrobial activities of synthesized AgNPs were evaluated using 3 different antibiotics against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria and Candida albicans yeast. Antifungal activity of AgNPs with antibiotics has been observed to be better than the antibiotics against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentrations were found to be 0.084, 0.337 and 0.021 mg mL(-1) for Escherichia coli, Staphylococcus aureus, and Candida albicans, respectively. The results revealed that AgNPs synthesized from maize leaf extract have antibacterial activity against Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus and antifungal activity against Candida albicans yeast, and that the produced AgNPs could be used in the production of biomedical products and in the pharmaceutical industry.
Investigating Antimicrobial Activity of Silver Nanoparticles Produced Through Green Synthesis Using Leaf Extract of Common Grape (Vitis Vinifera)
(Aloki Applied Ecological Research and Forensic inst Ltd, 2019) Acay, H.; Baran, M. F.; Eren, A.
In this study, a direct approach to fabricating silver nanoparticles (AgNPs) via the leaf extract of common grape (Vitis vinifera) has been demonstrated. The produced particles were found with a maximum wavelength of 452.47 nm, spherical shape and the crystal size of 18.53 nm through UV-Visible spectrophotometry, XRD (X-ray diffraction) and SEM (Scanning electron microscopy) characterization methods. Furthermore, the functional groups involved in the reduction were specified with FTIR (Fourier transform infrared spectroscopy), the elemental compounds were identified with EDX (Energy dispersive X-Ray spectroscopy) and the degradation points were determined with TGA-DTA (Thermal gravimetric analysis) methods. AgNPs were found to be effective against hospital pathogens, namely Gram-negative Escherichia coli ATCC 25922, Gram-positive Staphylococcus aureus ATCC 29213 and Candida albicans fungus at the concentrations of 0.314, 0.078 and 0.334 mu g mL(-1), respectively.