Biosynthesis and Characterization of Silver Nanoparticles Using King Oyster (pleurotus Eryngii) Extract: Effect on Some Microorganisms

Abstract

The integration of the principles of green chemistry into nanotechnology has become one of the key issues in nanotechnology research. Metal nanoparticle production, which does not contain toxic chemicals and does not harm the environment, needs to be developed to avoid adverse effects on medical applications. In this study, Pleurotus eryngii (PE) extract was used for preparation of silver nanoparticles (AgNPs). The presence of AgNP was understood that after adding 1 mM silver nitrate (AgNO3) to the fungus extract, the reaction turned from the open yellow to reddish brown. The analysis of samples taken at different times with the UV-Visible Spectrophotometer (UV-Vis) confirms the formation of PE-AgNPs. The Scanning electron microscopy-Energy Dispersive X-Ray Spectrum (SEM-EDX) analysis showed that spherical nanoparticles were formed. X-ray crystallography (XRD), analysis is calculated from Debye-Sherers inequality, in which PE-AgNP synthesized in the study was 18.45 nm in size. It has been demonstrated by using the minimum Inhibitory Concentration (MX) method in which AgNPs have strong antimicrobial activity.