Browsing by Author "Bilge, Ugur"

Now showing 1 - 3 of 3

Mechanistic Insights on Physiological, Biochemical, and Metabolite Profiling of Oryza Sativa Grown Under Drought Stress: A Strategic Coping Mechanism of Silicon Oxide Nanoparticles Treatment
(Springer, 2025) Bilge, Ugur; Dogan, Serap; Eren, Abdullah; Habib, Yawar; Faizan, Mohammad
Climate change is increasing the frequency of droughts, posing a significant threat to crop yields, particularly for drought-sensitive species like rice (Oryza sativa). Drought stress adversely impacts the physiological and biochemical functions of plants, ultimately resulting in lower productivity. This study aims to fill the knowledge gap concerning effective approaches to alleviate drought-induced damage and boost productivity in rice. We hypothesize that the application of silicon oxide nanoparticles (SiO2-NPs) can enhance drought tolerance in rice by influencing its physiological and biochemical responses. To evaluate this hypothesis, rice plants were cultivated under well-watered and drought-stressed 20% of polyethylene glycol (PEG; MW 6000) conditions using a completely randomized design (CRD) with four replications. The results revealed that applying various concentrations of SiO2-NPs (50 mg/L, 100 mg/L, and 200 mg/L) significantly improved growth and enhanced key physiological and biochemical traits such as protein content, photosynthetic rate, stomatal conductance, chlorophyll content, and antioxidant activity. It also significantly elevated Fv/Fm levels by 29%, 22%, and 16% respectively by 50 mg/L, 100 mg/L, and 200 mg/L concentrations of SiO2-NPs. Activity of several key antioxidant enzymes: superoxide dismutase (SOD) by 41%, 35%, and 33%, catalase (CAT) by 38%, 33%, and 31%, peroxidase (POD) by 47%, 41%, and 37%, and proline content by 29%, 26%, and 24%, under drought stress, respectively. Moreover, SiO2-NPs (50 mg/L, 100 mg/L, and 200 mg/L) alleviated drought-induced oxidative stress by reducing hydrogen peroxide (H2O2) level by 28%, 21%, and 15% and malondialdehyde (MDA) level by 31%, 23%, and 18%, respectively, compared to control plants. Furthermore, SiO2-NPs increased the concentration of total free amino acid (TFAA) and total soluble sugar (TSS) along with nitrogen (N), phosphorus (P), and potassium (K). The results of this study indicated that different concentrations (50 mg/L, 100 mg/L, and 200 mg/L) of SiO2-NPs could improve plant resistance and lessen the negative effects of drought stress. The post-harvest performance and reproductive stage of drought-stressed rice treated with SiO2-NPs require more research.
Diyarbakır Yöresindeki Bazı Tarım Topraklarının Hümik Asit İçerikleri ile Eser Elementler Arasındaki İlişkinin Çok Değişkenli İstatistiksel Yöntemlerle Değerlendirilmesi
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Duzgun, Mehmet; Eren, Abdullah; Bilge, Ugur; Ceylan, Ramazan; Selcuk, Ramazan; Duz, M. Zahir
There are important relationships between humic acid (HA) and the bioavailability, reactions and mobility of trace elements in the soil. For this reason, soils are tried to be improved chemically, biologically and physically with HA applications. In this study, the relationship of humic acid contents of 118 agricultural soil samples from Diyarbakir region with some trace elements (Al, As, Ba, Be, Cd, Fe, Mn, Pb, Sb, Sn, Se, V and P) was evaluated by multivariate statistical analysis. After the soil samples were solubilized by the microwave wet digestion method, the element contents were determined with the ICP OES (Inductively Coupled Plasma Optical Emission Spectrometer) device. SRM NIST 2586 was used as SRM (Standard Reference Material) for the accuracy of the method. Recovery values were found between 91.6% and 105.9% as a result of the analysis. Humic acid was extracted from soils by the International Society for Humic Substances (IHSS) method and determined using a shaker and centrifuge device. For the accuracy of the method, it was tested with Humic Acid Sodium Salt (HA-Na). Pearson correlation and partial correlation analysis were applied to the obtained data set. In addition, multivariate statistical analyses such as multiple regression HCA (Hierarchical Cluster Analysis) and PCA (Principal Component Analysis) were applied. Multiple regression analysis was performed according to the Step-wise method. Manganese and P (p< 0.01) were significant when HA was taken as the dependent variable. According to the Pearson correlation coefficient, the correlation between HA and As (r = -0.282**) in soil was negative and significant, while Fe (r = 0.185*), Mn (r = 0.273**)), Sn (r = 0.242*), Se (r = 0.325**) and P (r = 0.315**) were determined as positive and significant. In clustering and PCA analysis, HA, P Mn and Fe were found to be in the same group. The analyses have shown that HA has a positive effect on the plant nutrients in the soil.
Citation - WoS: 1
Citation - Scopus: 1
Unveiling the Ameliorative Effects of Soil-Mediated Nano-Biochar and Calcium Oxide Nanoparticles on Drought Tolerance in Oryza Sativa: Insights into Biochemical Responses, Reactive Oxygen Species, Photosynthetic Pigments and Nutrient Homeostasis
(Springer, 2025) Bilge, Ugur; Rajput, Vishnu D.; Eren, Abdullah; Yalcin, Mehmet; Habib, Yawar; Faizan, Mohammad
This study investigates the synergistic effects of calcium oxide nanoparticles (CaO NPs) and nano-biochar (nano-BC) on drought-stressed rice (Oryza sativa), a combination that has not been extensively explored in previous research. While individual applications of NPs or BC have been studied, the concurrent use of CaO NPs (as foliar spray) and nano-BC (as soil amendment) offers a novel integrative approach for enhancing drought resilience. The study demonstrates that, this combined application significantly mitigates drought-induced damage, as evidenced by improvements in physiological and biochemical traits. Notably, the treatment enhanced net photosynthetic rate (P-N) by 96.46%, stomatal conductance (gs) by 93.75%, and total soluble sugar (TSS) by 95.22% compared to drought-stressed plants. It also improved protein content, nitrogen accumulation, and transpiration rate. Additionally, reductions of 56% in malondialdehyde (MDA) and 59% in hydrogen peroxide (H2O2) indicate alleviation of oxidative stress. These findings provide new insights into the potential of nanomaterial-based interventions for sustainable rice cultivation under water-limited conditions, offering a promising strategy to improve crop resilience in the face of climate change.