Browsing by Author "Caglayan, Mehmet Veysi"

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The Detailed Chemical and Biological Analysis Of Ziziphora Clinopodioides Lam. Species Growing in Cultural and Natural Environments
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Cavusoglu, Mehmet; Yigitkan, Serkan; Yener, Ismail; Caglayan, Mehmet Veysi; Resitoglu, Baris; Akdeniz, Mehmet; Ertas, Abdulselam
The Lamiaceae family is an important resource for the pharmaceutical, food, cosmetic, and perfumery sectors. The species Ziziphora clinopodioides Lam., belonging to the Lamiaceae family, has been used in folk medicine since ancient times. The aim of the present study was to determine the total phenolic and flavonoid contents, antioxidant, cytotoxic and enzyme (AChE, BChE, tyrosinase, urease, elastase, collagenase and ACE) inhibition activities of ethanol extracts of the aerial parts of natural and cultivated samples of the species Z. clinopodioides. In addition, the aroma contents of the species were determined by GC-MS/FID, and the phytochemical composition of the ethanol extracts was determined by LC-MS/MS. According to the aroma analysis results, the major components of the culture sample of the Z. clinopodioides species were determined as pulegone (39.83%), cis- menthone (21.36%), trans-menthone (16.64%), while the major components of the natural sample were determined as pulegone (62.42%), neoisomenthol (5.93%) and cis-pulegone oxide (5.47%). According to LC-MS/MS results, quinic acid (25.841, 15.694 mg analyte g-1 extract, respectively), rosmarinic acid (6.804, 25.523), and acacetin (6.115, 10.764) were detected as major compounds in cultured and natural samples of the species. Also, hesperidin (5.725) was higher in culture sample. According to the enzyme activity results, it was determined that cultured and natural samples of the species showed moderate butyrylcholinesterase enzyme inhibition activity (inhibition %: 45.14 +/- 1.40; 43.57 +/- 0.73, respectively). In urease enzyme inhibition activity, it was determined that culture sample showed moderate activity (inhibition %: 43.64 +/- 0.39) but natural sample did not show activity, and culture sample showed high antihypertensive activity (inhibition %: 81.6 +/- 1.19). When the results were evaluated in general, it was determined that the cultured sample of the species showed higher antioxidant, butyrylcholinesterase, urease, and antihypertensive activity compared to natural sample.
Lc-ms/Ms Analysis and Biological Activities of Different Parts Of Ziziphora Capitata L.
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Yigitkan, Serkan; Cavusoglu, Mehmet; Caglayan, Mehmet Veysi; Yener, Ismail; Firat, Mehmet; Cavus Kaya, Eda; Ertas, Abdulselam
The Ziziphora species, classified under the Lamiaceae family, have a strong aromatic property. Ziziphora species have been used in folk medicine as sedative, gastric, aphrodisiac, bloating, and degassing. In the current study, the phenolic and flavanoid content of ethanol extracts of Ziziphora capitata L. species of flower, leaf, branch, mixed, and root parts was determined by the LC-MS/MS device. In addition, the antioxidant and cytotoxic activities of the extracts, as well as their inhibitory effects on enzymes (antihypertensive, AchE (acetylcholinesterase), BchE ( butyrylcholinesterase), elastase, tyrosinase, collagenase and urease), were determined. The LC-MS/MS results showed that quinic acid (25578, 5842, 25171, 14055, 10597 mu g g-1, respectively) was found in higher amounts in flower, leaf, branch, mixed, and root extracts of Z. capitata species compared to other components. Additionally, rosmarinic acid (17097 mu g g-1), cynaroside (8432), and hesperidin (8067) were found to be major components. It was observed that the flower extract of the species exhibited strong antioxidant activity (IC50: 37.18 +/- 1.36 mu g mL-1, 9.89 +/- 0.45, A0.5:16.27 +/- 0.02, respectively) in DPPH, ABTS and CUPRAC methods. It was concluded that the leaf extract of Z. capitata species had a strong cytotoxic effect on HT-29 (colon cancer cell line) (viability %: 9.26 +/- 0.69). It was observed that the root part of the species exhibited higher activity in butyrylcholinesterase (BChE) enzyme inhibition activity (inhibition %: 40.56 +/- 0.88) than other parts. It was determined that Z. capitata extracts did not show acetylcholinesterase, urease, tyrosinase, elastase, collagenase, and antihypertensive enzyme activity or showed low activity. As a result, it is thought that the flower extract of the Z. capitata species has better results in terms of the examined parameters, whereas the leaf extract needs to be subjected to more detailed in vitro and in vivo research conducted to be used in the pharmaceutical industry as a result of its cytotoxic effect against colon cancer cell lines.