Browsing by Author "Caliskan, Cemile Gunbegi"

Now showing 1 - 4 of 4

Bioactive Profile, Anticarcinogenic, Antimicrobial, Antidiabetic Effects, and in Silico Pharmacokinetic Properties of Astragalus Elatus
(Academic Press inc Elsevier Science, 2025) Ercan, Leyla; Akan, Hasan; Caliskan, Cemile Gunbegi
Because of the numerous useful components they contain, plants are significant resources that are being explored for their varied purposes in industry, food, and medicine. Recently, particularly endemic species have gained attention with the identification of their different ingredients and characteristics and the exploration of their precious features. This research assessed the endemic species Astragalus elatus's bioactive components utilizing UHPLC-Orbitrap (R)-HRMS, its antibacterial qualities utilizing the disc diffusion method, its cytotoxic impact on cervical cancer utilizing the MTT method, and its apoptosis effect by annexin V methods. Additionally, Astragalus elatus's antidiabetic activity was revealed by in silico and in vitro examinations of its inhibitory effect on the enzymes alpha-Amylase and alpha-Glucosidase. Consequently, it was discovered that A. elatus can be utilized in the treatment of cervical cancer and has effective cytotoxic and apoptotic effects on HeLa cells. Additionally, it was detected that A. elatus possesses antibacterial and anti-diabetic properties. Compared to gram-negative bacteria, A. elatus had a greater inhibitory effect on gram-positive bacteria. The pharmacokinetically significant characteristics and their tendency for chemical reactions of this plant's primary phenolic compounds were identified by utilizing DFT, molecular docking, and ADME/T.
Citation - WoS: 4
Citation - Scopus: 3
Comparison of chemical and antimicrobial properties of different nanoparticles synthesized from Verbascum x calcicolum Hub.-Mor. Hybrid
(Elsevier, 2024) Günbegi Çalışkan, Cemile; Caliskan, Cemile Gunbegi; Kilic, Murat; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Recent advances in nanoparticle synthesis have led to an increase in the use of environmentally acceptable plantderived nanoparticles, given their wide range of applications. Due to their stability and several advantageous characteristics, selenium nanoparticles also stand out in these investigations in addition to metallic nanoparticles like silver, gold, iron, and zinc. These nanoparticles' favorable qualities for human health, such as antioxidant and antibacterial activities, are being studied in addition to their chemical characteristics. This investigation aimed to produce zinc oxide nanoparticles (VcZnO) and selenium nanoparticles (VcSe) from hybrid Verbascum x calcicolum Hub.-Mor. And to examine their antioxidant and antimicrobial properties. Utilizing the disk diffusion method and well methods, its antibacterial capabilities were studied. In vitro antioxidant capabilities were also assessed utilizing the DPPH, FRAP, and CUPRAC techniques. As a result, it emerged that zinc oxide nanoparticles synthesized from hybrid Verbascum x calcicolum were effective on gram-positive bacteria and gram-negative bacteria such as Staphylococcus aureus (MIC = 160 mg/ml for VcZnO), Pseudomonas aeruginosa (MIC = 80 mg/ml for VcZnO), Klebsiella pneumoniae (MIC = 40 mg/ml for VcZnO and, MIC = 120 mg/ml for VcSe), Escherichia coli (MIC = 20 mg/ml for VcZnO). While selenium nanoparticles are more favorable in terms of antioxidant capabilities, zinc nanoparticles demonstrate stronger antibacterial properties than selenium nanoparticles. Thus, it has been discovered that the use of different nanoparticles of various plants can be more efficient in different applications. It has also been found that Verbascum x calcicolum is a material that can be used successfully in nanoparticle synthesis.
Citation - WoS: 4
Citation - Scopus: 6
Evaluation of Extra Virgin Olive Oil Compounds Using Computational Methods: in Vitro, Admet, Dft, Molecular Docking and Human Gene Network Analysis Study
(Bmc, 2025) Unsal, Velid; Yildiz, Resit; Korkmaz, Aziz; Mert, Basak Dogru; Caliskan, Cemile Gunbegi; Oner, Erkan; Yıldız, Reşit; Unsal, Velid; Korkmaz, Aziz; Günbegi Çalışkan, Cemile; 09.03. Department of Nutrition and Dietetics/ Beslenme ve Diyetetik Bölümü; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
This study investigates the phenolic compounds (PC), volatile compounds (VC), and fatty acids (FA) of extra virgin olive oil (EVOO) derived from the Turkish olive variety "Sar & imath; Ulak", along with ADMET, DFT, molecular docking, and gene network analyses of significant molecules identified within the EVOO. Chromatographic methods (GC-FID, HPLC) were employed to characterize FA, PC, and VC profiles, while quality parameters, antioxidant activities (TAC, ABTS, DPPH) were assessed via spectrophotometry. The analysis revealed a complex composition of 40 volatile compounds, with estragole, 7-hydroxyheptene-1, and 3-methoxycinnamaldehyde as the primary components. Hydroxytyrosol, tyrosol, oleuropein, apigenin, ferulic acid, and vanillic acid emerged as main phenolic constituents, with hydroxytyrosol and apigenin exhibiting high bioavailability. Molecular docking highlighted oleuropein and pinoresinol as compounds with strong binding affinities, though only hydroxytyrosol, apigenin, and pinoresinol fully met Lipinski and other drug-likeness criteria. DFT analysis showed that oleuropein and pinoresinol have notable dipole moments, reflecting polar and asymmetrical structures. KEGG enrichment analysis further linked key molecules like oleuropein and apigenin with pathways related to lipid metabolism and atherosclerosis, underscoring their potential bioactivity and relevance in health-related applications.
Citation - WoS: 1
Citation - Scopus: 1
Phytochemical Profile, Antimicrobial, Antioxidant, Anti-Xanthine Oxidase, and Anti-Elastase Activities of Centaurea Hyalolepis: an in Silico and in Vitro Analysis
(Wiley-v C H verlag Gmbh, 2025) Günbegi Çalışkan, Cemile; Caliskan, Cemile Gunbegi; Akan, Hasan; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
This study aimed to examine the antibacterial, antioxidant, and enzyme activity of Centaurea hyalolepis, as well as the total phenolic and carotenoid contents, and volatile and phenolic components. Gas chromatography-mass spectrometry was used for the volatile component analysis of C. hyalolepis, while liquid chromatography-mass spectrometry was used for the phenolic component analysis. Furthermore, the antimicrobial activity was evaluated using disc diffusion and microdilution techniques. The antioxidant capacity was investigated in vitro using four distinct methods (2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, cupric ion-reducing antioxidant capacity, and N,N-dimethyl-p-phenylene diamine). Additionally, both in vitro and in silico studies were carried out to determine the inhibitory effect on xanthine oxidase and elastase. Consequently, it was determined that C. hyalolepis has antioxidant properties and is a highly efficient antibacterial agent against Bacillus megaterium, Pseudomonas aeruginosa, Bacillus subtilis subsp. spizizenii, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, and Candida albicans. Additionally, C. hyalolepis contains many volatile compounds, such as 2-methyl propanoic acid, 3,5-octadien-2-one, 2,6-dimethyl cyclohexanol, hexanal, pentanal, anethole, and beta-cyclocitral. It also has a large number of phenolic components, including chlorogenic acid, apigenin 7-glucuronide, quinic acid, and 4-hydroxybenzoic acid. Moreover, C. hyalolepis was shown to inhibit xanthine oxidase and elastase.