Browsing by Author "Demirtaş, Selim"

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Diyabetik Rat Testis Dokusunda Ku70, Sırt1 ve Sırt6 Proteinlerinin Rolü
(2024) Demirtaş, Selim; Ersoy, Onur; Kızılay, Gülnur
Diyabetik erkek infertilite/subfertilitesi, günümüzde diyabetin önemli komplikasyonları arasında yerini almıştır. Diyabetik erkek infertilitesinin altında yatan moleküler mekanizmalar henüz tam olarak aydınlatılamamıştır. Çalışmamızda; KU70, SIRT1 ve SIRT6 proteinlerinin bu mekanizmalar içerisindeki rolünün aydınlatılmasına katkı sağlanması amaçlanmıştır. Stabil laboratuvar koşulları altında tuttuğumuz Sprague Dawley erkek sıçanlardan, kontrol grubu (n=8) ve tek doz 50 mg/kg streptozotosin uygulanan diyabet grubu (n=8) olmak üzere iki grup oluşturulmuştur. Diyabet grubunda; ışık ve elektron mikroskobik incelemelerimizde diyabetik testis dokusunda şimdiye kadar literatürde yer alan tüm histopatolojik bulgular gözlenmiş, morfometrik olarak da seminifer tübül çaplarının azaldığı tespit edilmiştir. Çalışmamızda, KU70, SIRT1 ve SIRT6 ifadeleri incelendiğinde KU70 diyabet grubunda istatistiksel anlamlılıkla artarken, SIRT1 ve SIRT6 ifadelerinin diyabetik grupta, kontrol grubuna göre anlamlı şekilde düştüğü belirlenmiştir. Sonuç olarak, diyabetik erkek hastalarda karşılaşılan subfertilite/infertilite olgularında, KU70, SIRT1 ve SIRT6 proteinlerinin önemli bir rolü olabileceği ve bu proteinlerin, kantitatif başka çalışmalarla da desteklenerek öneminin vurgulanması gerektiği kanısındayız.
Effects of Gilaburu Fruit Extract on Cardiomyopathy in Diabetic Rats
(2025) Seker, Ugur; Gokdemir, Gul Sahika; Demirtaş, Selim
Objective: The effects of diabetes on cardiomyopathy have been well documented in the literature. However, the effects of gilaburu fruit, which possesses antioxidant and anti-inflammatory properties, on diabetic cardiomyopathy (DCM) have not been adequately investigated. This study aimed to investigate the effects of gilaburu extract on DCM in diabetic rats. Method: Twenty-one male Wistar rats were divided into three groups. Diabetes was induced in the diabetic group with a single intraperitoneal (i.p.) injection of streptozotocin (STZ) at a dose of 50 mg/kg. The treatment group received gilaburu extract (200 mg/kg, i.p.) dissolved in phosphate buffer saline (PBS) for 37 days, starting one week before diabetes induction. Serum CK-MB and CRP levels, cardiac tissue MDA, SOD, and IL-1ß levels, as well as histopathological changes such as hypertrophy, vacuolization, and fiber disarray, were evaluated. Results: Serum CK-MB and CRP levels were significantly higher in the diabetic group compared to the control group. The diabetic group also exhibited marked myocardial hypertrophy, vacuolization, and fiber disarray, along with increased MDA and IL-1ß levels and decreased SODactivity. In the treatment group, CK-MB and CRP levels were significantly reduced, histopathological alterations were markedly attenuated, and MDA, IL-1ß, and SOD levels improved to values close to those of the control group. Conclusion: Gilaburu extract exhibits both biochemical and morphological protective effects against diabetic cardiomyopathy. These findings suggest that gilaburu may be a promising therapeutic agent for the management of diabetic cardiomyopathy.
Tartrazine-Induced Nephrotoxicity via Oxidative and Genotoxic Pathways in Rats: Regulatory Insights and the Nephroprotective Role of Curcumin
(Academic Press Inc Elsevier Science, 2026) Demirtaş, Selim; Tan, Fazile Canturk; Keskin, Cumali; Varol, Salih; Yavaş, Mehmet Cihan; Cantürk Tan, Fazile
This study evaluated tartrazine-induced nephrotoxicity and the protective effects of curcumin in rats. Thirty-five male Wistar albino rats were assigned to five groups (n = 7): control; tartrazine 10 mg/kg/day; tartrazine 100 mg/kg/day; tartrazine 10 mg/kg/day + curcumin 20 mg/kg/day; and tartrazine 100 mg/kg/day + curcumin 20 mg/kg/day. After 21 days, blood and kidney samples were analyzed for biochemical, oxidative, genotoxic, and histopathological changes. High-dose tartrazine significantly elevated serum urea and creatinine levels compared with controls (urea, p = 0.033; creatinine, p < 0.001), indicating renal dysfunction. Curcumin co-treatment mitigated these elevations. Total antioxidant status (TAS) was elevated by tartrazine exposure but decreased with curcumin supplementation (p < 0.001), total oxidant status (TOS) showed a non-significant increasing trend and was reduced by curcumin. Compared to the control group, MDA levels decreased with low-dose tartrazine and increased with high-dose tartrazine, while curcumin supplementation increased levels (p < 0.05). Comet assay and histopathological analyses confirmed dose-dependent DNA and tissue damage, both of which were alleviated by curcumin. Overall, short-term tartrazine exposure may induce renal biochemical, oxidative, and genotoxic alterations in rats under experimental conditions, particularly at doses exceeding the ADI level. The antioxidant properties of curcumin may mitigate the negative effects of food dyes.