Browsing by Author "Ercan, L."

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Citation - Scopus: 7
Bioactive Components, Antioxidant Capacity, and Antimicrobial Activity of Berberis Crataegina Fruit
(Elsevier B.V., 2024) Ercan, L.
Research highlighting the benefits of plants provides possibilities for their application as food preservatives and in complementary medicine. The fruits of the wild shrub species Berberis crataegina, which humans eat, were examined in this study. The objective has been to ascertain the fruit's bioactive constituents, including minerals, phenolic compounds, fatty acids, and volatile components, in addition to its antioxidant and antibacterial properties. The antioxidant capacity of B. crataegina fruit was investigated in vitro by DPPH, DMPD, ABTS, FRAP, CUPRAC, and Total antioxidant methods. Additionally, the disk diffusion technique was used to assess its antibacterial impact on fungus and bacteria. It was determined that the fruit of B. crataegina is rich in Ca (27399 ppm), K (9634 ppm) Mg (5052 ppm), and Fe (470 ppm), contains essential oils such as alpha-linolenic acid (37%), and oleic acid (14%). Strong antioxidant qualities were demonstrated by B. crataegina, particularly in the ABTS and CUPRAC assays. In addition to 47 volatile compounds consisting mostly of aldehydes, fatty acids, and terpenes, 52 phenolic compounds were detected, including chlorogenic acid, rutin hydrate, and procyanidin B2. It also showed antimicrobial properties on S. aureus, P. aeruginosa, and K. pneumoniae. © 2024 Elsevier Ltd
Cytotoxic Effects of Verbascum Andrusii on Lung Cancer: Anti-Elastase, Anti-Xanthine Oxidase, Antimicrobial, Antioxidant Properties, Bioactive Profile, and Integrated in Vitro and in Silico Assessments
(Elsevier Ltd, 2026) Ercan, L.; Calıskan, C.G.
Verbascum andrusii is an endemic species that grows in the Southeastern Anatolia Region. This study sought to figure out the total amount of phenolic molecules, the total amount of carotenoids, phenolic compounds, volatile compounds, antioxidant and antimicrobial activity, the inhibition effect on xanthine oxidase and elastase enzymes of V. andrusii, and to examine its cytotoxic effect on A549 (lung carcinoma) cells. For this, V. andrusii's volatile and phenolic components were analyzed employing the HS-SPME technique and UHPLC-Orbitrap®-HRMS, respectively. Additionally, the antibacterial behavior by the disc diffusion method and the in vitro antioxidant capacity by four distinct techniques (DPPH, ABTS, CUPRAC, and DMPD) were assessed. Furthermore, the inhibitory effect of its on the enzymes elastase and xanthine oxidase was assessed both in vitro and in silico. Pharmacokinetic features and activities of bioactive components of V. andrusii were assessed in silico by ADME/T, molecular docking, and density functional theory (DFT). As a result, it was revealed that V. andrusii showed a cytotoxic effect on A549 cells (IC50: 130.40 μg/mL) and had antioxidant, antimicrobial (on the microorganisms E. coli, K. aerogenes, P. aeruginosa, C. albicans, S. aureus, and K.pneumoniae), antiaging (antielastase IC50: 450.10 μg/mL), and antihyperuricaemia (antixanthinoxidase IC50: 239.02 μg/mL) properties. In addition, the effect of the components of this plant on the biological properties of the plant was evaluated by in silico studies. It was concluded that in silico investigations of diosmetin, luteolin, luteolin 7-rutinoside, apigenin, quinic acid, benzoic acid, anethole, and D-limonene, which are the main components of V. andrusii, confirmed the experimental studies. © 2026 Elsevier Ltd