Browsing by Author "Evcil, Murat"

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Development, Characterization and Application of Chitosan-Based Formulation Incorporating Crataegus Orientalis Extract for Food Conservation
(Jomard Publishing, 2025) Evcil, Murat; Kurt, Barış; Baran, Ayse; Mouhoub, Anouar; Karakaplan, Mehmet
In this investigation, chitosan-based films loaded with plant extracts from Crataegus orientalis (CR) were elaborated and evaluated in terms of structural, physicochemical and antimicrobial properties. Firstly, the CR extract was characterized by LC-MS/MS showing an abundance of Protocatechuic acid (56.82 μg/g) and Chlorogenic acid (67.13 μg/g). Then, the extract was incorporated into chitosan-based films at different concentrations (CHCR1-3). Findings revealed modifications in FTIR and XRD graphs as well as SEM micrographs following the incorporation of CR extract confirming the changes in the matrix structure and texture. Moreover, the addition of the extract reduced the transparency, swelling ratio, water solubility and moisture content potencies while increasing considerably the material thickness reaching 2.5-fold as regards CHCR3. Furthermore, Thermogravimetric findings showed three stages of degradation for CH control and CHCR2, with mass loss due to water evaporation and glycerol breakdown. Ultimately, CHCR bioformulations showed significant antimicrobial activity against Escherichia coli, Staphylococcus aureus and Candida albicans, especially in the case of the CHCR3, which was verified by the application as a preservative coating for lemon fruit. These encouraging results highlight the potential utilization of CHCR bioformulation as coating/packaging for perishable food products. © 2025 Elsevier B.V., All rights reserved.
In Silico Exploration of Plant Extracts as Ache Inhibitors: Insights From Molecular Dynamics and MM/GBSA Analysis for Alzheimer's Drug Development
(2025) Kurt, Barıs; Baran, Ayşe; Evcil, Murat
Alzheimer's disease is a long-term neurological disorder that affects memory and other cognitive abilities. Physostigmine is a drug still used in treating symptoms associated with this disease, with its primary mechanism of action being AChE inhibition. AChE plays a crucial role in cholinergic neurotransmission, and its inhibition has been linked to the improvement of symptoms in Alzheimer's disease. In this study, 34 phytochemicals detected through LC-MS/MS analysis of 13 plant species were investigated as potential alternative drug candidates to physostigmine. For this purpose, docking studies followed by molecular dynamics simulations and MM/GBSA energy calculations were performed. The results revealed that 24 out of 34 phytochemicals were either very close to physostigmine (MM/GBSA binding affinity: -26.102 kcal/mol) or better AChE inhibitors. Additionally, it was determined that physostigmine increased the flexibility of the molecule when bound to the AChE enzyme, a unique result compared to our drug candidates. Our research emphasizes the potential of plant-derived compounds as AChE inhibitors and presents promising candidates for future drug development studies. Furthermore, physostigmine's property of increasing enzyme flexibility offers a new perspective in drug design and indicates that the role of this feature in therapeutic efficacy needs to be examined in more detail.
Preparation and Characterization of Silver-Loaded Magnetic Activated Carbon Produced From Crataegus Monogyna for Antimicrobial and Antioxidant Applications
(Wiley-v C H verlag Gmbh, 2025) Ertas, Erdal; Dogan, Serap; Baran, Ayse; Baran, Mehmet Firat; Evcil, Murat; Kurt, Baris; Aslan, Kadir Sinan; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Secondary metabolites from several plant species have been used to cure various illnesses. Current advances allowed green synthesis nanoparticle manufacturing of metal salts from plant sources. This study involves binding activated carbon obtained from the Crataegus monogyna plant to magnetic nanoparticles and coating the resulting magnetic activated carbon nanocomposite with Ag ions (CMAC/MNPs-Ag) to produce a biomedical nanobiological material. Various techniques such as SEM, EDX, XRD, FTIR, UV-Vis, VSM, DLS, and zeta potential were used to characterize synthesized nanocomposites. CMAC/MNPs-Ag nanocomposite demonstrated activity in several processes of antioxidant activity tests. DPPH and CUPRAC activities of CMAC/MNPs-Ag nanocomposite were measured as 90.21 +/- 0.42 and 46.73 +/- 0.108 mg TE/g, respectively, while total phenolic content was measured as 27.15 +/- 0.381 mg GAE/g. Finally, the antibacterial activity of CMAC/MNPs-Ag nanocomposite was evaluated against Escherichia coli and Staphylococcus aureus by microdilution and disk diffusion techniques. The antimicrobial activity of CMAC/MNPs-Ag nanocomposite was determined using microdilution and disk diffusion techniques. For Escherichia coli, microdilution and disk diffusion were measured as 1.17 mu g mL-1 and 12 mm, respectively, while for Staphylococcus aureus, microdilution and disk diffusion were measured as 2.34 mu g mL-1 and 10 mm, respectively.
Synthesis of Plant-Derived Selenium Nanoparticles from Lankaran-Astara Tea (Camellia sinensis L.) Plant and Evaluation of Their Activities on Different Enzymes
(Jomard Publishing, 2025) Alasgarova, Narmina; Baran, Ayse; Yıldıztekın, Mahmut; Ganbarov, Dashgin Sh; Babayeva, Safura; Güneş, Zubeyir; Evcil, Murat
In this study, selenium nanoparticles (SeNPs) were produced utilizing a green technique with Camellia sinensis (green tea) extract, which is high in polyphenols, flavonoids and catechins, all of which are known antioxidants and bioactive. For this reason, the tea plant can be used in the treatment and prevention of many diseases. Selenium nanoparticles (SeNPs) produced by green synthesis method with plant extracts provide more advantages compared to traditional physical and chemical methods. In this study, Camellia sinensis L. (tea) plant was used as a reducing agent in the synthesis of selenium nanoparticles (SeNPs). The obtained tea extract selenium nanoparticles (CS-SeNPs) were tested on various enzymatic activities such as anticholinesterase (AChE), butyrylcholinesterase (BChE), urease, α-amylase, α-glucosidase and tyrosinase inhibitor activities. The results revealed that CS-SeNPs exhibited strong inhibitory effects especially on AChE and BChE activities. (AChE IC50: 25.33 ± 0.78 µg/mL, BChE IC50: 17.53 ± 0.27 µg/mL). Additionally, CS-SeNPs exhibited the strongest inhibitory activity against urease enzyme with an IC50 value of 13.72 ± 0.18 µg/mL. CS-SeNPs also showed strong inhibition on α-amylase enzyme with an IC50 value of 25.98 ± 0.83 µg/mL, while the inhibitory effect on tyrosinase was determined as 31.40 ± 0.51 µg/mL. In compared to conventional inhibitors such as galantamine, acarbose, thiourea and kojic acid, the CS-SeNPs showed weaker but still significant inhibitory action in all enzyme models. The current study is significant because it investigates the environmentally friendly synthesis of selenium nanoparticles using Camellia sinensis and assesses their potential as multifunctional enzyme inhibitors, which could help to develop alternative therapeutic agents for neurodegenerative and metabolic diseases. © 2025 Elsevier B.V., All rights reserved.