Browsing by Author "Keskin, C."

Now showing 1 - 7 of 7

Advances in Nano Vaccines: Covid-19
(Bentham Science Publishers, 2023) Asefy, Z.; Nasibova, A.; Hoseinnejhad, S.; Selimoğlu, A.; Baran, M.F.; Keskin, C.
Nanovaccines are considered a new approach in vaccination methodology specially for Covid-19 infection. Nanovaccines are more effective than conventional vaccines; Because ofhumoral and cellular immune responses which are simultaneously induced. Nano vaccines are assumed to upregulate the immune system as well as infection prevention. They are probably promising candidates for chronic autoimmune diseases such as multiple sclerosis, rheumatoid arthritis, AIDS, and COVID-19 infectious. Based on this, we will describe the different working mechanisms of nanoparticles. In addition, applicable nano vaccines which have been approved for COVID-19 therapy Covid 19 are described. Antigen-carrying nanoparticles can affect the immune response and significantly enhance cell-T cytotoxic response. Nanoscale particles can improve vaccine efficiency because of their biomedical benefits. These properties include Small size, which allows better penetration into tumors and more half-life tumor cells. Current vaccines, however, are required to re-formulate almost because of gradual antigen modifications. More ever these vaccines do not protect against mutations and the low half-life of current vaccines due to limitations of current technologies. Nano vaccine formulation improvements have been required to induce a widespread and potent immune response. In this review, we provide an overview ofthe types and applications of nanoparticles in vaccines and their outstanding properties that made them alternatives for Covid-19 treatment. © 2023, Bentham Books imprint.
Green-Synthesized Nanoparticles for Biomedical Sensor Technology
(Elsevier, 2024) Baran, A.; Baran, M.F.; Ipek, P.; Eftekhari, A.; Keskin, C.; Atalar, M.N.; Alma, M.H.
Sensor technology is an integral part of the many cost-effective and efficient factors possible in modern medical devices. Biosensors have good potential as they are easy, scalable, and effective in manufacturing processes. Nanotechnology has become one of the promising technologies applied in all fields of science. Biotechnologically produced metallic nanoparticles (NP) attract attention in scientific applications and technology platforms due to their extensive applications in biomedical and physiochemical fields. In recent years, the side effects caused by the use of synthetic drugs and the medical and economic problems caused by them have made the use of plants popular again. Ecofriendly, nontoxic metal-based NPs (such as gold, silver, palladium, manganese, and zinc) smaller than 100nm in size can be synthesized with extracts obtained from plants by different methods. As the sizes and shapes of NPs change, the physical, chemical, bioactive, optical, electrical, catalytic, and toxicity properties of the particles also change. Biological synthesis, also known as green synthesis, is a practical method to obtain NPs easily and ecologically without the need for high pressure, high-temperature values, and toxic chemicals. Green synthesis of NPs is carried out using different biomaterials such as bacteria, fungi, yeast, viruses, microalgae, and plant biomass/essence. Plant-mediated biosynthesis of metallic NPs occurs through biomolecules containing organic functional groups in the plant. Nanobiosensors, analytical devices combining a biologically sensitive element with a nanostructured transducer, are widely used for the molecular detection of biomarkers associated with the diagnosis of diseases and the detection of infectious organisms. Nanobiosensors show certain advantages over laboratory and many field methods due to their inherent specificity, simplicity, and rapid response. In this study, advancements in the development of nanobiosensors are illuminated. Considering all these aspects, it can be said that nanobiosensors enable diagnostic tools with increased sensitivity, specificity, and reliability for medical applications. © 2025 Elsevier Inc. All rights reserved.
Hydrogen peroxide-induced oxidative damage in human mononuclear leukocyte: the anti-genotoxic effects of H. Perforatum extract on DNA damage
(WILEY-BLACKWELL, 2015) Aktepe, N.; Keskin, C.; Yukselten, Y.; Sunguroglu, A.
…
In-Vitro Antioxidant, Cytotoxic, Cholinesterase Inhibitory Activities and Anti-Genotoxic Effects of Hypericum Retusum Aucher Flowers, Fruits and Seeds Methanol Extracts in Human Mononuclear Leukocytes
(Iranian Journal of Pharmaceutical Research, 2017) Keskin, C.; Aktepe, N.; Yükselten, Y.; Asuman,; Boğa, M.
The present study investigates the antioxidant, anticancer, anticholinesterase, anti-genotoxic activities and phenolic contents of flower, fruit and seed methanol extracts of Hypericum retusum AUCHER. The amounts of protocatechuic acid, catechin, caffeic acid and syringic acid in methanol extracts were determined by HPLC. Total phenolic content of H. retusum seed extract was found more than fruit and flower extracts. The DPPH free radical scavenging activity of flower and seed methanol extracts showed close activity versus BHT as control. Among three extracts of H. retusum only flower methanol extract was exhibited considerable cytotoxic activities against to HeLa and NRK-52E cell lines. Moreover, seed methanol extract showed both acetyl and butyrl-cholinesterase inhibitory activity. The highest anti-genotoxic effects were seen 25 and 50 μg/mL concentrations. In this study, the extracts showed a strong antioxidant and anti-genotoxic effect. The seed extract was more efficient- than extracts of fruit and flowers. Our results suggest that the antioxidant and anti-genotoxic effects of extracts depend on their phenolic contents. Further studies should evaluate the in-vitro and in-vivo the benefits of H. retusum seed methanol extracts. © 2017 by School of Pharmacy.
Interactions of Nanoparticles and Biological Systems
(Jomard Publishing, 2024) Khalilov, R.; Nasibova, A.; Kavetskyy, T.; Kazımlı, L.; Keskin, C.; Bayramova, M.
This article investigates the effects of licorice root (Glycyrrhiza L.) syrup on laboratory rats exposed to ionizing gamma radiation, focusing on its potential radioprotective properties. Ionizing gamma radiation induces oxidative stress, characterized by an increase in free radicals and subsequent tissue damage, which can contribute to the development of various diseases such as neurodegenerative, cardiovascular, diabetes and kidney diseases. The body’s antioxidant defense mechanisms, including superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA), play crucial roles in combating oxidative stress. Historically recognized for its medicinal properties, licorice root may mitigate the harmful effects of radiation. The research employed Electron Paramagnetic Resonance (EPR) spectroscopy to analyze structural and functional changes in various biological systems under the influence of radiation and other stressors. This investigation aims to provide insights into the protective effects of licorice syrup, contributing to the understanding of potential therapeutic strategies against radiation-induced oxidative damage. As a continuation of the experiments, the levels of antioxidant enzymes in the rat’s erythrocytes were measured using various methods. Biochemical analyses conducted on the erythrocytes of the rats indicated positive effects of licorice syrup on the levels of SOD, CAT and MDA. © 2024, Jomard Publishing. All rights reserved.
Study of Endogenous Paramagnetic Centers in Biological Systems From Different Areas
(John Wiley and Sons Inc, 2021) Nasibova, A.; Khalilov, R.; Abiyev, H.; Kavetskyy, T.; Trubitsin, B.; Keskin, C.; Eftekhari, A.
Plant leaves (Eldar pine (Pinus eldarica M.), fig (Ficus carica L.), and olive (Olea europaea L.)), collected in territories with different ecological conditions, of the Absheron Peninsula (Azerbaijan Republic) were studied by electron paramagnetic resonance spectroscopy (EPR). The generation of nanophase iron oxide magnetic particles in biological systems under the influence of stress factors was revealed. It was found that the process of biomineralization plays a role in the formation of biogenic iron oxide magnetic nanoparticles in plants and the generation of magnetite crystals in biological tissues, and stress factors have a stimulating effect on this phenomenon. Copyright © 2021 Aygun Nasibova et al.
Trace Metal Determination in the Medicinal Plant Hyoscyamus (Solanaceae) by Inductively Coupled Plasma Optical Emission Spectrometry
(Perkin - Elmer Corp., 2014) Keskin, C.; Kisin, E.; Yavuz, M.
The concentrations of Cd, Ni, Cu, Pb, Cr, Fe, B, and A1 were determined in four different medicinal plants of the Hyoscyamus species: Hyoscyamus reticulums L, Hyoscyamus leptocalyx STAPF, Hyoscyamus aureus L, and Hyoscyamus albus L by ICP-OES. By considering the sensitivity of ICP-OES with respect to the trace level determination of Cd, Ni, Cu, Pb, Cr, Fe, B, and Al in the studied plant samples and the limitations of sample amount possible in a microwave digestion procedure, the ashing method was applied before analysis and proved the quantitative measurements of the elements.The accuracy of the method was verified by comparison to the certified reference sample NCSZC 73014 Tea Leaves. The RSD values were between 0.14-10.4%, while the error values were in the 90-107% range. The results show that with the proposed method higher concentrations of Ni, Cr, Pb, Fe, and B were determined in H. aureus. Interestingly, the concentration of Cu in H. reticulatus was approximately 48.5 times higher than the mean Cu values of the other three Hyoscyamus species, while the Pb concentration levels in H. aureus were 17.8 times higher than in the H. reticulatus L, H. leptocalyx STAPF, and H. albus L species.