Browsing by Author "Koc, Ibrahim"

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In Vitro Cytotoxicity and Antioxidant Evaluation of Oak (Quercus sp.) and Hazelnut Shell (Corylus avellana L.) Pyrolized Wood Vinegar: MTT Assay and CUPRAC Results
(Springer, 2025) Koc, Ibrahim; Sabancilar, Ilhan; Kaya, Seckin; Mendes, Mehmet; Keskin, Cumali; Eftekhari, Aziz
Wood vinegar, also known as pyroligneous acid, is an organic liquid byproduct of wood pyrolysis, rich in compounds such as acetic acid, phenols, and ketones. This study aimed to evaluate the antioxidant and anticancer properties of wood vinegar derived from oak (Quercus sp.) residues (WVO) and hazelnut shells (Corylus avellana L.) (WVH) under in vitro conditions. Method: Wood vinegars obtained from oak trash and hazelnut shell waste were tested for biological activity. The antioxidant capacity was assessed using the copper(II) reducing antioxidant capacity (CUPRAC) method. Cytotoxic effects were evaluated using the MTT assay on healthy human endothelial cells (HUVEC) and two cancer cell lines (HT29-colon adenocarcinoma and U2OS-osteosarcoma) at concentrations ranging from 1.25% to 20% (v/v) over 24, 48, and 72-hour exposure periods. The results revealed significant interactions between vinegar type, concentration, and exposure duration. Notably, the 10% and 20% (v/v) concentrations produced significantly different absorbance values compared to lower concentrations across all time points. A dose-dependent decrease in cell viability was observed, particularly in the cancer cell lines, while healthy cells exhibited relatively higher resistance. The study demonstrates that wood vinegar exhibits promising antioxidant and selective anticancer properties, with cytotoxic effects being more pronounced at higher concentrations. These findings suggest that wood vinegar may offer potential as a natural anticancer agent. Further research is warranted to elucidate the underlying mechanisms and to explore their possible therapeutic applications.
Effects of Pesticide and Wood Vinegar Applications on Surface-Dwelling Arthropods in the Wheat
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2026) Koc, Ibrahim; Yardim, Erdal Necip
This study was conducted to determine the effects of certain pesticides (herbicides, fungicides) used in the wheat agro-ecosystem and wood vinegar (WV) produced from Broiler chicken bedding on soil-dwelling arthropods. The research was carried out under the ecological conditions of Mu & scedil; province during the 2014-2016 production seasons. Pesticide and WV applications were carried out using a backpack sprayer as follows: First, the application of pesticides (fungicides and herbicides); Secondly, corresponding to each pesticide application, WV applications were made at concentrations of 0.5%, 1%, 2%, 3%, 4%, and 5% (v/v). Finally, a control application was conducted using only tap water in response to the pesticide and WV applications. Arthropod sampling was conducted using pitfall traps. The arthropods captured in the traps were considered in terms of their family (Carabidae, Gryllidae, Araneidae, and Formicidae), Order (Opiliones), and the number of individuals of other arthropods. Simple Correspondence Analysis Technique was used for data analysis. As a result, it was observed that the applications affected the number of arthropods considered. Additionally, it was determined that there was an increase in the number of arthropods captured in traps in the 2016 season compared to 2015. In comparison to the control application, the average number of arthropods captured in the traps for the Carabidae family was highest in the 1% WV application (42.49 individuals) and lowest in the pesticide application (18.44 individuals); for the Araneidae family, it was highest in the 5% WV application (23.97 individuals) and lowest in the pesticide application (14.66 individuals); for the Formicidae family, it was highest in the 1% WV application (38.41 individuals) and lowest in the 0.5% WV application (17.69 individuals); for the Opiliones order, it was highest in the 4% WV application (24.55 individuals) and lowest in the pesticide application (10.69 individuals); and for other arthropods, it was highest in the pesticide application (35.66 individuals) and lowest in the 1% WV application (23.05 individuals). According to statistical analyses, significant relationships were identified between the applications and the number of arthropods captured in the pitfall traps.