Browsing by Author "Kolukcu, Vildan"

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An Animal Model of Reperfusion in Ischemic Corporal Tissue and the Effect of Sugammadex on Oxidative Injury Parameters
(MRE Press, 2025) Kolukcu, Vildan; Yalcin, Kenan; Eroglu, Askin; Gevrek, Fikret; Gurpinar, Ahmet Burak; Unsal, Velid
Background: The aim of this study was to analyze the effect of sugammadex on ischemia-reperfusion injury in corporal tissue. Methods: Eighteen male Wistar Albino strain rats were divided into three groups. Group 1 served as the control group. A priapism model was induced in rats in Group 2 and 3. Rats in Group 3 were additionally administered 4 mg/kg sugammadex intravenously immediately after reperfusion. All rats underwent penectomy for histopathological and biochemical evaluations, and blood samples were collected. Results: In Group 2, total oxidant status (TOS) and malondialdehyde (MDA) levels were significantly higher compared to other groups (p < 0.001 and p = 0.009, respectively). A substantial reduction in both TOS and MDA levels was found in Group 3 (p <0.001 and p = 0.043, respectively). Group 2 exhibited significantly lower activities of a glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) compared to Group 1 (p = 0.043 and p = 0.024, respectively). In contrast, Group 3 showed significant increases in both antioxidant enzyme activities (p = 0.036 and p = 0.034, respectively). Similarly, the total antioxidant status (TAS) was dramatically higher in Group 3 compared to Group 2 (p < 0.001). Microscopic examinations revealed improvements in vasocongestion, edema, desquamation and inflammation scores in Group 3 compared to Group 2 (p < 0.001). Conclusions: Biochemical and histopathological findings suggest that, sugammadex significantly mitigates oxidative damage parameters in corporal tissue affected by ischemia-reperfusion injury.
Citation - WoS: 2
Citation - Scopus: 1
Effects of Ketamine on Penile Tissues in an Experimental Priapism Model in Rats
(Turkish Assoc Trauma Emergency Surgery, 2024) Gevrek, Fikret; Fırat, Fatih; Unsal, Velid; Tapar, Hakan; Karaman, Tugba; Karaman, Serkan; Yalçın, Kenan; Kolukcu, Vildan; Balta, Mehtap Gurler
BACKGROUND: This study aimed to evaluate the histopathological and biochemical effects of ketamine on penile tissues following ischemia-reperfusion injury induced by priapism. METHODS: Twenty-four male rats were randomized into three groups. Group 1 served as the control group. Group 2 underwent the priapism model to induce ischemia-reperfusion injury. Group 3, the treatment group, experienced a similar ischemia-reperfusion model as Group 2; additionally, 50 mg/kg of ketamine was administered intraperitoneally just before reperfusion. Blood biochemical analyses and penile histopathological evaluations were performed. RESULTS: In Group 3, significant improvements were observed in all histopathological scores, including desquamation, edema, inflammation, and vasocongestion compared to Group 2 (p<0.001). Blood biochemical analyses showed that the malondialdehyde (MDA) levels were recorded as 10 in Group 2, with a significant decrease in Group 3 (p=0.013). Similarly, proinflammatory cytokine levels, including interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), were found to be suppressed in Group 3 compared to Group 2 (p=0.003, p=0.022, and p=0.028, respectively). Antioxidant enzyme activities, such as glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), were higher in Group 3 compared to Group 2 (p=0.016 and p=0.024, respec- tively). CONCLUSION: Ketamine is an effective anesthetic agent in alleviating the effects of penile ischemia-reperfusion injury.
Citation - WoS: 6
Citation - Scopus: 6
Etomidate Alleviates Ovarian Ischemia-Reperfusion Injury in Rats
(Turkish Assoc Trauma Emergency Surgery, 2024) Katar, Muzaffer; Gevrek, Fikret; Unsal, Velid; Tapar, Hakan; Karaman, Tugba; Karaman, Serkan; Balta, Mehtap; Kolukcu, Vildan
BACKGROUND: This study investigates the protective effects of etomidate against oxidative damage in an experimental model of ovarian ischemia-reperfusion injury. METHODS: A total of 24 female rats were randomized into three groups. Group 1 served as the control. Group 2 underwent an ovarian torsion/detorsion procedure. Group 3 underwent similar procedures as Group 2; additionally, 4 mg/kg of etomidate was administered intraperitoneally 30 minutes before ovarian detorsion. Blood samples were analyzed for lipid peroxidation, pro-inflamma- tory cytokine levels, and antioxidant enzyme activity.Furthermore, histopathological scoring was performed to evaluate tissue damage in the ovaries. RESULTS: Biochemical analysis of blood samples revealed reductions in pro-inflammatory cytokines, including interleukin-1 Beta (IL- 1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), in Group 3 compared to Group 2 (p=0.005, p=0.016, and p<0.001, respectively). Additionally, a decrease in malondialdehyde (MDA) levels was observed in Group 3 compared to Group 2 (p<0.001). In contrast, activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), were signifi- cantly increased in Group 3 compared to Group 2 (p=0.031 and p=0.001, respectively). Furthermore, Group 3 demonstrated notable reductions in histopathological scores for follicular degeneration, vascular occlusion, bleeding, and inflammation compared to Group 2 (p<0.001, p<0.001, p<0.001, and p=0.001, respectively). CONCLUSION: Etomidate alleviates ischemia-reperfusion injury in a rat ovarian torsion-detorsion model by improving both histo- pathological and biochemical outcomes.
Citation - WoS: 1
Citation - Scopus: 1
Is There Any Effect of Lidocaine on Ischemia/Reperfusion Injury in Testicular Torsion? An Experimental Study
(Turkish Assoc Trauma Emergency Surgery, 2024) Gevrek, Fikret; Fırat, Fatih; Unsal, Velıd; Kılınç, Fatih; Tapar, Hakan; Karaman, Serkan; Yalçın, Kenan; Kolukcu, Vildan; Karaman, Tugba; Balta, Mehtap Gurler
BACKGROUND: This experimental study aimed to evaluate the potential protective effects of lidocaine on ischemia-reperfusion injury resulting from testicular torsion/detorsion in rats. METHODS: A total of 18 male rats were randomized into three groups. Group 1 served as the control group. Group 2 was designed to evaluate testicular ischemia-reperfusion injury using a torsion/detorsion model. In Group 3, the treatment group, a similar ischemia- reperfusion model was used as in Group 2. Additionally, lidocaine at a dose of 15 mg/kg was administered intraperitoneally five minutes before reperfusion. Blood biochemical analyses and testicular histopathological evaluations were conducted. RESULTS: Blood biochemical analysis showed that malondialdehyde (MDA) and protein carbonyl (PC) levels were significantly higher in Group 2 compared to the other groups (p<0.001 and p=0.008, respectively). Proinflammatory cytokine levels, including interleu- kin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha), were lower in Group 3 than in Group 2 (p<0.001, p=0.007, and p=0.026, respectively). Antioxidant enzyme activities, including glutathione peroxidase (GSH-Px) and superoxide dis- mutase (SOD), were higher in Group 3 compared to Group 2 (p=0.005 and p=0.025, respectively). Histopathological evaluations revealed significant improvements in all testicular damage scores, including hemorrhage, edema, vasocongestion, and inflammation in Group 3 compared to Group 2 (p=0.015, p=0.035, p=0.015, and p=0.034, respectively). Additionally, there was a notable improvement in the Johnsen score in Group 3 compared to Group 2 (p=0.034). CONCLUSION: Lidocaine, an effective local anesthetic, significantly alleviates the effects of testicular ischemia-reperfusion injury.
Citation - WoS: 1
Citation - Scopus: 1
Possible Protective Effect of Remifentanil Against Testicular Ischemia-Reperfusion Injury
(Walter de Gruyter GmbH, 2024) Katar, Muzaffer; Gevrek, Fikret; Fırat, Fatih; Unsal, Velıd; Tapar, Hakan; Karaman, Tugba; Kuyucu, Yunus Emre; Kolukcu, Vildan; Balta, Mehtap Guerler
Objectives: This study aims to evaluate the protective effi- cacy of remifentanil against testicular ischemia-reperfusion injury. Methods: The study included 24 male rats. The rats were randomized into three groups: Group 1 was the control group. Group 2 was subjected to a testicular torsion/ detorsion model. Group 3 underwent similar procedures and additionally received remifentanil (0.6 μg/kg/min) intrave- nously for the first 20 min of reperfusion. Blood samples were taken for biochemical analyses, and orchiectomy was performed for histopathologic examination. Results: Biochemical analysis of blood samples showed a significant increase in antioxidant enzyme activity, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in Group 3 compared to Group 2 (p:0.004 and p:0.002, respectively). There was a dramatic decrease in the levels of proinflammatory cytokines, including interleukin-1 beta (IL-1 Beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) in Group 3 compared to Group 2 (p:0.001, p:0.046, and p:0.004, respectively). Similarly, malondialdehyde (MDA) levels decreased in Group 3 compared to Group 2 (p:0.004). Histopathologic examination of Group 3 rats showed positive changes in inflammation, hemorrhage, edema, and conges- tion levels compared to Group 2 (p<0.001). Similarly, there was a positive effect on the Johnsen and Cosentino score in Group 3 compared to Group 2 (p:0.001 and p<0.001, respectively). Conclusions: In our study, it has been documented that remifentanil protects against testicular ischemia-reperfusion injury.
The Potential Renoprotective Effect of Sugammadex in Renal Ischemia-Reperfusion Injury
(Galenos Publishing House, 2025) Gevrek, Fikret; Fırat, Fatih; Unsal, Velid; Kolukcu, Vildan; Sahin, Ahmet Tugrul; Balta, Mehtap; Yancı, Asiye; Genc, Ali; Bala, Mehtap Gurler; Gurpinar, Ahmer Burak
Objective: We aimed to evaluate the effectiveness of sugammadex on renal tissue for against ischemia-reperfusion injury. Methods: Twenty-one Wistar albino strain female rats were divided into three groups. The first group functioned as the control cohort for comparison. In Groups 2 and 3, a renal ischemia-reperfusion model was established. Moreover, following the cessation of ischemia, the rats in Group 3 were intravenously administered sugammadex at a dose of 4 mg kg-1. Blood and tissue samples were subsequently collected for analysis. Results: Biochemical analyses revealed a notable increase in the enzymatic activities of glutathione peroxidase and superoxide dismutase in Group 3 relative to Group 2 (P < 0.001 and P=0.015, respectively). Additionally, the concentration of malondialdehyde was found to be significantly reduced in Group 3 relative to Group 2 (P=0.004). Group 3 exhibited a substantial decrease in tumor necrosis factor-alpha, interleukin 6, and interleukin 1 beta levels when compared to Group 2 (P=0.021, P=0.006, and P=0.016 respectively). Group 2 exhibited the highest concentrations of neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 (P < 0.001 and P=0.015, respectively). Similarly, the histopathologic tissue damage was the most prominent in Group 2 (P < 0.001). Conclusion: Sugammadex plays a protective role against ischaemia-reperfusion injury in renal tissue.