Browsing by Author "Tumer, Mehmet Kemal"

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Citation - WoS: 3
Citation - Scopus: 5
A study of chewing muscles: Age-related changes in type I collagen and matrix metalloproteinase-2 expression
(ScienceDirect, 2020) Çiçek, Mustafa; Tumer, Mehmet Kemal; Unsal, Velid
Objective: In this study, the aim was to investigate the biochemical, physiological and histological changes that occur in masticatory muscles of the masticatory system with aging. Design: In this study, 14 BALB/c mice were used. Animals were divided into two equal groups of seven. Group I was organized as the group of young animals (n = 7) and Group II as the group of adult animals (n = 7). After routine histological follow-up was performed, the tissues were embedded in paraffin. 4-5 μm thick cross-sections were taken from paraffin-embedded tissues and they were stained with Haemotoxylin and Eosin Type I collagen and Matrix metalloproteinase-2 (MMP-2) immunohistochemically. Results: It was observed that there was a decrease and shrinking in blood vessels due to aging. In young mice, Type I collagen and MMP-2 immunoreactivity in the masseter muscle tissue showed low staining, while Type I collagen and MMP-2 immunoreactivity in the temporal muscle tissue showed moderate staining. Type I collagen and MMP-2 immunoreactivity were significantly higher in the masseter and temporal muscles of elderly mice (p = 0.001). In the H-score evaluation, MMP-2 immune reactivity was significantly lower in young mice than in older mice (p = 0.001). Conclusion: It was determined that severe pain complications and functional losses are likely to occur with the increase of degeneration due to aging of masticator muscles.
Citation - WoS: 1
Citation - Scopus: 1
Investigation of the Relationship Cellular and Physiological Degeneration in the Mandible with AQP1 and AQP3Membrane Proteins
(Walter de Gruyter GmbH, 2020) Cicek, Mustafa; Unsal, Velid; Tumer, Mehmet Kemal
Objective: In this study, we aimed to investigate the changes in the levels of oxidative stress and antioxidant enzymes on the mandibular bone caused by the expression of aquaporin-1 and aquaporin-3 proteins. Material and method: 14 Balb/C white mice were divided into two groups of seven, based on whether they are young or old. Mandibular tissue samples were taken for biochemical and histological analysis. Results: Findings of our study has shown that, AQP-1 and AQP-3 immunoreactivity significantly decreased in mandibular bone tissues of aged mice in comparison to younger mice (p < 0.05). MDA and AOPP levels, which are the indicators of oxidative stress, increased in elderly mice and antioxidant defense system SOD enzyme activity was decreased (p < 0.05). The TNF-alpha cytokine level, which is the indicator for inflammations, was found to be higher in older mice than in young mice (p < 0.05). Conclusion: As a result, it was observed that cellular damage, disruption in water - electrolyte balance and increased inflammation that occur during the natural process of aging had caused serious and irreversible disturbances.